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All Journal Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Perikanan Universitas Gadjah Mada Jurnal Intek Akuakultur Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health Jurnal Penyuluhan Perikanan dan Kelautan Jurnal Abdi Insani Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Penelitian Belida Indonesia Journal of Fisheries & Marine Halal Studies and Society Journal of Vocational in Aquaculture (JAVA)

Dinamella Wahjuningrum

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

Author-ID : 254184

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Social Sciences Veterinary

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Potensi Bakteri Proteolitik Aeromonas caviae NU-4 dan Aeromonas sp. NU-8 sebagai Pengendali Pertumbuhan Microcystis aeruginosa BT-02 pada Ikan Mas (Cyprinus carpio) Mulyeti, Encah Ewi; Rachmania Mubarik, Nisa; Wahjuningrum, Dinamella
0853-8670
Publisher : PBI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.511 KB)

Sianobakteri merupakan kelompok fitoplankton yang umum dijumpai di perairan tawar di seluruh dunia. Sianobakteri menghasilkan toksin mikrosistin yang dihasilkan oleh Microcystis aeruginosa yang menyebabkan kematian ikan. Penelitian ini bertujuan mengetahui potensi bakteri proteolitik yang berasal dari saluran pencernaan ikan nila GIFT dalam menghambat pertumbuhan M. aeruginosa BT-02. Isolat bakteri proteolitik asal saluran pencernaan ikan nila yang menghambat pertumbuhan M. aeruginosa BT-02, yaitu isolat i.e Aeromonas caviae NU-4 and Aeromonas sp. NU-8. Indeks penghambatan bakteri NU-4 (1,71) terhadap M. aeruginosa lebih besar daripada NU-8 (1,34). Mekanisme penghambatan belum diketahui. Aplikasi Microcystis aeruginosa BT-02 pada ikan mas tidak menyebabkan kematian ikan, tetapi menimbulkan beberapa perubahan histopatologi pada hati dan usus ikan mas.Kata kunci: Bakteri proteolitik, Aeromonas sp., Microcystis aeruginosa, ikan mas, tes toksisitas

Aktivitas Antibakteri Ekstrak Kapang Laut Nodulisporium sp. KT29 terhadap Vibrio harveyi Sri Hariati; Dinamella Wahjuningrum; Munti Yuhana; Kustiariyah Tarman; Irzal Effendi; Fazril Saputra
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Nodulisporium sp. KT29 merupakan salah satu kapang endofit yang diisolasi dari alga merah Eucheuma edule, tidak bersifat toksik, dan bersifat antibakteri. Penelitian ini bertujuan mengevaluasi aktivitas antibakteri ekstrak kapang laut Nodulisporium sp. KT29 terhadap Vibrio harveyi. Penelitian dilakukan dengan beberapa tahapan yaitu kultivasi kapang, ekstraksi senyawa aktif, pengujian aktivitas antibakteri, kromatografi lapis tipis (KLT), bioautografi, serta pengamatan kerusakan sel bakteri Vibrio harveyi. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan dipekatkan dengan rotary evaporator. Ekstrak Nodulisporium sp. KT29 diuji terhadap bakteri Vibrio harveyi pada konsentrasi 0,125; 0,25; 0,5; 1 dan 2 mg/disc. Ekstrak Nodulisporium sp. KT29 membentuk zona hambat tertinggi pada konsentrasi 2 mg sebesar 45,33±0,71 mm. Hasil uji KLT ekstrak Nodulisporium sp. KT29 menunjukkan adanya 7 spot retardation factor (Rf) dengan tiga komponen warna yaitu cokelat, kuning dan ungu. Uji bioautografi menghasilkan 2 spot zona hambat yaitu 16 mm dan 13 mm pada Rf 0,94 dan 0,14. Pengamatan menggunakan SEM menunjukkan bahwa kerusakan pada morfologi sel Vibrio harveyi dengan pemberian ekstrak Nodulisporium sp. KT29 yang ditandai dengan lisis.

Pengaruh Pengunaan Probiotik Lactobacillus brevis dan Prebiotik Oligosakarida (Fructooligosakarida-Galaktoologosakarida) Terhadap Gambaran Darah Patin Siam (Pangasionodon Hypophtalmus) yang Diinfeksi Aeromonas Hydrophila Yuke Eliyani; Widanarni Widanarni; Dinamella Wahjuningrum
Jurnal Penyuluhan Perikanan dan Kelautan Vol 7, No 1 (2013)
Publisher : Program Studi Penyuluhan Perikanan Politeknik Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33378/jppik.v7i1.37

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian probiotik Lactobacillus brevis, prebiotik oligosakarida (Fructooligosakarida- Galaktooligosakarida) terhadap nilai hemoglobin, hematokrit serta diferensial leukosit patin siam (Pangasionodon hypophthalmus) yang diinfeksi bakteri Aeromonas hydrophila. Uji yang dilakukan meliputi persiapan bakteri probiotik dan A.hydrophila, analisis prebiotik (oligosakarida), uji in vitro, dan uji in vivo. Parameter uji yang diamati selama penelitian adalah hemoglobin, hematokrit, serta diferensial leukosit. Penelitian terdiri dari lima perlakuan dengan masing-masing tiga ulangan, yaitu kontrol (+), kontrol (-), pemberian probiotik (Pro), pemberian prebiotik (Pre), pemberian probiotik ditambah prebiotik (Sin). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor. Data dianalisis dengan sidik ragam (ANOVA) pada selang kepercayaan 95%. Perbedaan antar perlakuan dilanjutkan dengan uji Duncan menggunakan program Xl-stat. Hasil pengamatan pada parameter hemoglobin, hematokrit, serta diferensial leukosit, berbeda nyata (p<0.05) dengan kontrol pada beberapa waktu pengamatan.

UTILIZATION OF MARINE FUNGAL Nodulisporium sp. KT29 METABOLITES TO IMPROVE THE PRODUCTION PERFORMANCE OF MARINE CULTURE OF WHITE SHRIMP Fazril Saputra; Dinamella Wahjuningrum; Kustiariyah Tarman; Irzal Effendi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

This study aimed to determine the best dose of administration of marine fungal Nodulisporium sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp Litopenaeus vannamei post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of Nodulisporium sp. KT29 metabolites, dietary of feed containing Nodulisporium sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P<0.05). The administration of marine fungal Nodulisporium sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.

Potensi Bakteri Proteolitik Aeromonas caviae NU-4 dan Aeromonas sp. NU-8 sebagai Pengendali Pertumbuhan Microcystis aeruginosa BT-02 pada Ikan Mas (Cyprinus carpio) Encah Ewi Mulyeti; Nisa Rachmania Mubarik; Dinamella Wahjuningrum
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 3 (2012): October 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i3.148

RESISTANCE AGAINST Aeromonas hydrophila INFECTION AND GROWTH OF SECOND GENERATION (F2) AFRICAN CATFISH [Clarias gariepinus] USING SELECTED MOLECULAR MARKERS Alimuddin Alimuddin; Fadhila Maharani Putri; Dinamella Wahjuningrum; Dian Hardiantho; Ade Sunarma; Sri Nuryati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 2 (2018)
Publisher : SEAMEO BIOTROP

Aeromonas hydrophila is a pathogenic bacteria that causes mass mortality in catfish. In previous studies, specific pathogen resistant (SPR), A. hydrophila-resistant African catfish first generation (F1) has been cultivated by marker assisted selection using the major histocompatibility complex (MHC) 1 as a molecular marker. In this study, growth performance, inheritance of the MHC DNA marker in the second generation (F2) of catfish and disease resistance against A. hydrophila infection were observed.Â The F2 progenies were produced by crossing F1 fish between themselves. Nursery was performed in 80-L glass aquaria, 4 replications for each cross, at the same initial density, for 2 months of rearing. The results showed that daily growth rate of F2 progenies from the SPR broods was significantly higher than those from broods without the marker.Â Results of the PCR analysis showed that average number of F2 progenies from SPR broods carrying the MHC marker was about 91% higher than that of control.Â After the fish reached about 12 cm body length, they were challenged by intramuscularly injecting of 0.1 mL A. hydrophila (LD50: 106 CFU ml-1) for 7 days. Results of challenge test showed that survival of F2 offspring from the crosses of SPR broods (77.2%) was about two times higher than those from brood without MHC marker (38.3%).Â Differential leukocyte count supported the high resistance of F2 progenies from F1 broods having MHC I marker against A. hydrophila infection. In conclusion, African catfish farming carrying MHC marker potentially have higher productivity and reduces fish lose due to infection by A. hydrophila.

Aplikasi Bakteri Probiotik melalui Pakan Buatan untuk Meningkatkan Kinerja Pertumbuhan Udang Windu (Penaeus monodon) Widanarni Widanarni; Dinamella Wahjuningrum; Fiska Puspita
Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Vol. 2 No. 1 (2012): Jurnal Sains Terapan, Volume 2, Nomor 1, Tahun 2012
Publisher : IPB University

This research was conducted to study the effect of probiotic bacteria application through artificial feed on growth performance of tiger shrimp. The research was carried out for 42 days with four treatments and three replications, those were control, SKT-b (feed + SKT-b probiotic), 1 UB (feed + 1 UB probiotic) and commercial (feed + commercial probiotic). Feed and probiotic bacteria were mixed in ratio of 3:1 (weight : volume) by adding 3 % of white egg as a binder. Then the test feed was analyzed by proximate analyses and compared by using control feed. Tiger shrimps with initial weight of 0.04 ± 0.005 g were reared in 50 cm x 40 cm x 40 cm aquaria at the density of 15 shrimps per aquarium. Feeding was carried out four times a day at 08.00, 12.00, 16.00, and 22.00. The weight of tiger shrimp were observed once every 2 weeks, whereas the water quality and the total abundance of bacteria in media were monitored at the beginning, middle and end of the research.The result showed that theadministration of the probiotic bacteria through artificial feed produced better growth rate than control. Application of 1 UB probiotic gave the best results by producing 9.03% growth rate and 1.35feed conversion ratio. Whereas, survival rates for all treatments were not significantly different from the range of values between 91.11% - 97.78%.Keywords: probiotic bacteria, feed, tiger shrimp

Effect of cinnamon, Cinnamomun burmanii, leaves extract for non specific immune response in striped catfish Pangasianodon hypophthalmus (Sauvage, 1878) infected by Aeromonas hydrophila nFN Safratilofa; Dinamella Wahjuningrum; Dedi Jusadi; Mia Setiawati
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.58

Aeromonas hydrophila causes Motile Aeromonads Septicemia disease in catfish. Phytopharmaca can be performed as an alternative to increasing the nonspecific immune response of catfish. One of the potentially phytopharmaca is cinnamon, Cinnamomum burmanii. This study was conducted to evaluate the potential of cinnamon leaves to increase the nonspecific immune responses of infected catfish by A. hydrophila. Cinna-mon leaf extract was given through fish diet at doses 0.5% and 1% for fourteen days. The challenge test was performed on the day 15. The method used in this study was a completely randomized design with six treatments and three replications. The six treatments were the preventive treatment of 0.5% (A) and 1% (B), the controlling treatment of 0.5% (C) and 1% (D), negative control (K -) and positive control (K +). The results showed that the controlling treatment of 0.5% (C) was the best treatment with the phagocytic activi-ty of blood cells of 77.59±2.50% and respiratory burst activity of 0.072 ± 0.004 after challenge test. More-over, the survival rate of fish was 100% in the C treatment, whereas in the positive control (K+) only 63.33%. Abstrak Bakteri Aeromonas hydrophila dapat menyebabkan penyakit Motile Aeromonads Septicaemia pada ikan patin. Penggunaan fitofarmaka dilakukan sebagai alternatif peningkatan respon imun non spesifik ikan patin. Salah satu bahan fitofarmaka yang dapat dimanfaatkan adalah tumbuhan kayu manis Cinnamomum burmanii. Penelitian ini bertujuan untuk mengevaluasi potensi daun kayu manis dalam meningkatkan respon imun non spesifik ikan patin yang diinfeksi A. hydrophila. Daun kayu manis diberikan dalam bentuk ekstrak melalui pakan dengan dosis 0,5% dan 1% selama 14 hari. Uji tantang dilakukan pada hari ke-15. Metode dalam penelitian ini menggunakan rancangan acak lengkap (RAL) dengan enam perlakuan dan masing-masing tiga ulangan. Perlakuan pencegahan 0,5% (A) dan 1% (B), perlakuan pengendalian 0,5% (C) dan 1% (D), serta kontrol negatif (K-) dan positif (K+). Hasil penelitian menunjukkan bahwa perlakuan C merupakan hasil terbaik dalam meningkatkan respon imun non spesifik pada ikan patin pascauji tantang. Hal tersebut terlihat dari peningkatan aktifitas fagositik 77,59±2,50% dan aktifitas ledakan respiratori 0,072±0,004 ikan patin dan juga pada dosis ini tingkat kelangsungan hidup ikan mencapai 100% sementara pada kontrol positif tingkat kelangsungan hidup ikan hanya 63,33%.

Efektivitas pemberian zeolit, arang aktif, dan minyak cengkeh terhadap hormon kortisol dan gambaran darah benih ikan patin Pangasionodon hyppophthalmus pada pengangkutan dengan kepadatan tinggi [Effectivity of utilization of zeolite, activated charcoal, and clove oil to cortisol hormone on the high density transportation system of juvenile of Pangasionodon hyppophthalmus] Eddy Supriyono; Ruspindo Syahputra; M. Faisol Riza Ghozali; Dinamella Wahjuningrum; Kukuh Nirmala; Anang Hari Kristanto
Jurnal Iktiologi Indonesia Vol 11 No 1 (2011): Juni 2011
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v11i1.157

This study was aimed to determine the effect of combination of zeolite, activated charcoal and clove oil for maintaining water quality in the transport medium of the fish, so as to minimize the stress level which can be known through determination of cortisole hormone level and blood characteriestic of the fish and also to minimize mortality rate of the fish . The study was conducted with a completely randomized design. The fish used was juvenile iridescent shark catfish with an average weight of 2 g / fish. Dose zeolite, activated charcoal and salt were used that B (20 g zeolite + 10 g activated carbon), C (20 g zeolite + 10 g activated charcoal + 3 ppm clove oil), D (20 g zeolite + 10 g activated charcoal + 6 ppm clove oil), E (20g zeolite + 10 g activated charcoal + 9 ppm clove oil), F (20 g zeolite + 10 g activated charcoal + 12 ppm clove oil), and A(without zeolite, activated charcoal and oil cloves). The results showed the combination of 20 g zeolite + 10 g activated charcoal + 9 ppm clove oil in the sealed-transporatation system for 72 hours gave the best results, by reaching of the lowest levels of NH3 0.0389±0.004 mgl-1, CO2 50.42 mgl-1, highest survivale rate of 83.11% and had lower stress levels that presented by hormone cortisol level and blood characteristics as close to the normal fish. AbstrakPenelitian ini bertujuan untuk menentukan pengaruh pemberian zeolit, arang aktif dan minyak cengkeh dalam memper-tahankan kualitas air media pada pengangkutan ikan berkepadatan tinggi , sehingga dapat meminimalisasi tingkat stres yang dapat diketahui dari tingkat konsentrasi hormon kortisol dan gambaran darah ikan dan dapat meminimalisasi ting-kat kematian ikan. Penelitian dilakukan pada skala laboratorium dengan Rancangan Acak Lengkap. Ikan yang diguna-kan yaitu ikan patin dengan bobot rata-rata 2 g/ekor. Dosis zeolit, arang aktif dan garam yang digunakan yaitu B (20 g zeolit + 10 g arang aktif), C (20 g zeolit + 10 g arang aktif + 3 ppm minyak cengkeh), D (20 g zeolit + 10 g arang aktif + 6 ppm minyak cengkeh), E (20 g zeolit + 10 g arang aktif + 9 ppm minyak cengkeh), F (20 g zeolit + 10 g arang aktif + 12 ppm minyak cengkeh), dan A (tanpa zeolit, arang aktif dan minyak cengkeh). Hasil penelitian menunjukkan pem-berian 20 g zeolit + 10 g arang aktif + 9 ppm minyak cengkeh dalam pengepakan sistem tertutup selama 72 jam mem-berikan hasil terbaik, yaitu, kadar NH3 terendah mencapai 0,0389±0,004 mgl-1, CO2 50,42 mgl-1, SR tertinggi sebesar 83,11% dan memiliki tingkat stres yang lebih rendah baik dilihat dari konsentrasi hormon kortisol terendah dan gambaran darah mendekati ikan normal.

CONTROL OF Vibrio alginolyticus INFECTION IN ASIAN SEA BASS Lates calcarifer USING AMBON BANANA PLANT POWDER Musa paradisiacal THROUGH THE FEED Hamida Pattah; Dinamella Wahjuningrum; Munti Yuhana; Widanarni Widanarni
Indonesian Aquaculture Journal Vol 15, No 2 (2020): (December, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.2.2020.85-91

Asian sea bass is one of economically important commodities in aquaculture. However, its culture often challenged by vibriosis infection which resulted in lower production. The study aimed to evaluate the effectiveness of the ambon banana plant powder application in feed as an effort to control the disease caused by Vibrio alginolyticus infection. Asian sea bass with total length of 7.48 ± 0.45 cm. Five different treatments were applied in this experiment, i.e. positive control, negative control, prevention, curative, and antibiotic application. The challenge test is carried out on the 15th day. Challenge test was performed with intramuscularly injection of V. alginolyticus at the cell density of 106 CFU/fish. The results showed the amount of feed consumption after the challenge test was highest in the curative and antibiotic control. However, these results were not significantly different (P>0.05) among preventive and curative treatments but were significantly different from controls (P<0.05). Fish treated with supplemented feed showed the highest total erythrocytes, total leukocytes, hemoglobin, respiratory burst, and lysozyme activity compared to controls. IL-1b gene expression increased after the challenge test with the highest level of expression in the curative treatment. It can be concluded that the administration of ambon banana plant powder (3 g/100 g diet) could control of V. alginolyticus infection in Asian sea bass and resulted 70.00% survival rate on prevention and 83.33% on curative treatments.

Co-Authors , Rahman, , , Ranta, , Ade Sunarma Afiff , Usamah Agus Oman Sudrajat Aldy Mulyadin Alimuddin Aminatul Zahra Anang Hari Kristanto Angela Mariana Lusiastuti Ardana Kurniaji Astari, Belinda Bagus Ansani Takwin Bambang Riyanto Dadang Kurniawan Danella Austraningsih Puspa Nazar DEDI JUSADI Dendi Hidayatullah Dendi Hidayatullah, Dendi Dewi Nurhayati Diah Ayu Lestari, Diah Ayu Dian Eka Ramadhani Dian Hardiantho Eddy Supriyono Efianda, Teuku Reza Eka Hidayatus Solikhah Elizabeth Waturangi, Diana Encah Ewi Mulyeti Erni Susanti Fadhila Maharani Putri Fajar Maulana . Faoziyatunnisa, Nurul Fiska Puspita Gustilatov, Muhamad Hamida Pattah Harton Arfah Hasan Nasrullah Hasanah, Mulyati Hendriana, Andri Herawati Rasid Hidayat, Acep Muhamad Iis Diatin Iis Widiani Ikhsan Khasani Ikhsan Khasani Iman Rusmana Inem Ode Iqbal Kurniawinata, Mohamad Irawan, D Y Irzal Effendi Julie Ekasari Karno Setyotomo Kautsar, Badar Kukuh Nirmala Kustiariyah Tarman Laely Nuzullia M. Faisol Riza Ghozali M. Zairin Junior M.A. Lidaenni Mia Setiawati Muhamad Ali MUHAMMAD AGUS SUPRAYUDI Muhammad Arif Mulya Muhammad Umar Al-Faruqi Muharram Nur Ikhsan Mulyadin, Aldy MUNTI YUHANA Nasrullah, Hasan nFN Safratilofa NISA RACHMANIA MUBARIK Nurbariah Nurbariah Nuzullia, Laely Ode, Inem Pratama, Ahmad Trio Puguh Widagdo Puji Hastuti, Yuni Putra, The Best Akbar Esa Putri Shandra Ramhirez Putri Utami, Putri Ramhirez, Putri Shandra Retno Astrini Ririn Nurul Fauziah, Ririn Nurul Rudi, Mad Ruspindo Syahputra Sahrul Alim Sari Anggraeni, Sukma Sri Hariati Sri Nuryati Sri Nuryati Sukenda Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Tatag Budiardi Taufiq Abdullah Tiara Puspa Anjani Tri Rezeki, Nanda Vinasyam, Apriana Wahyu Ramadhan Wesly Pasaribu WIDANARNI WIDANARNI Widanarni Widanarni Wildan Nurussalam Wira H Saputra Y. Hadiroseyani Yan Evan Yan Evan Yani Aryati Yonvitner - Yuke Eliyani Yuke Eliyani Zulhelmi, Arif

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