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All Journal Jurnal Hortikultura Indonesia (JHI) JURNAL TEKNOLOGI LINGKUNGAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Journal of Tropical Crop Science

Diyah Martanti, Diyah

Pusat Penelitian Biologi-LIPI, Cibinong Science Centre, Jl. Raya Bogor Km. 46, Cibinong 16911

Author-ID : 507484

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

Published : 11 Documents Claim Missing Document

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ANALISA KESTABILAN GENETIK PISANG KEPOK âUNTI SAYANGâ HASIL MIKROPROGASI DENGAN MARKA RAPD DAN ISSR [Genetic Stability Analyses of Micropropagated Pisang Kepok âUnti Sayangâ by RAPD and ISSR Markers] Poerba, Yuyu Suryasari; Imelda, Maria; Martanti, Diyah
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) markers were used to evaluate the genetic stability of micropropagated plants of âPisang Kepok Unti Sayangâ at various stage of in vitro sub-cultures and in vivo plant material.All RAPD and ISSR profiles from micropropagated plants were monomorphic and similar to those of field grown control plants until stage tenth of sub cultures (V1S10). No variation was detected within the micropropagated plants, except for C12 (V1S44),G7 (V1S48 ), I11 and I12 (V1S10).RAPD and ISSR marker were both could be used to test the genetic stability of micropropagated bananas using the developed protocol.

ANALISA GENETIK PISANG HIBRID DIPLOID BERDASARKAN MARKA RAPD (Random Amplified Polymorphic DNA) [Genetic Analysis of Diploid Banana Hybrids Based on RAPD Markers] Martanti, Diyah; Poerba, Yuyu Suryasari; Herlina, Herlina
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Banana breeding by crosses is one way to get new cultivars with a better quality. This study aims to verify molecular characteristic of diploid hibrid bananas i.e MDRK, SNMT and MDMT using Random Amplified Polymorphic DNA (RAPD) markers. Ten RAPD primers have been selected to detect the differences in molecular pattern from all three diploid hybrid bananas. The results showed that the hybrid combination of MDRK, SNMT and MDMT produced bands that shared some similarities with each parents by 43.83%, 51.25% and 47.21% respectively as the number of bands from type I, III and IV markers. Type III marker is the important marker for identification of male parents. Type VII marker (non-parent bands) was detected from hybrid MDRK, SNMT and MDMT by 18 (20.22%), 6 (7.5%) and 11 (12.64%) respectively. Marker of type VII can be used to identified hybrid because they are present only in offspring. The highest polymorphic bands was obtained by SNMT (83.9%) and the lowest obtained was performed by MDMT (71.25%). From this result, RAPD markers can be used as a tool to detect the differences of molecular pattern of diploid hybrid bananas.

PENGARUH MUTAGEN ETIL METAN SULFONAT (EMS) TERHADAP PERTUMBUHAN KULTUR IN VITRO ILES-ILES {Amorphophallus muelleri Blume) Poerba, Yuyu S; Leksonowati, Aryani; Martanti, Diyah
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Amorphophallus muelleri Blume (Araceae) is one of 27 Amorphophallus species occur wild in Indonesia (Sumatera, Java, Floresand Timor). The species is valued for its glucoman content for use in food industry (heathy diet food), paper industry, pharmacyand cosmetics. The cultivation of A. muelleri is hampered by limited genetic quality of seed. The species is triploid (2n=3x=39),the seed is developed apomictically. and pollen production is low. The species is only propagated vegetatively. This may explainthat the species is difficult to breed conventionally and genetic variabillity in the exiting landaraces cultivars is rather limited.Induced mutation using ethyl methan sulfonate is one of techniques to increase genetic variation. The present research is aimed todetermine Lethal Dosage (LD) 50% and 75% of EMS and to study effects of EMS on growth of A, muelleri in vitro cultures for usein induced mutation program. Results of the experiment showed that LD-50 and LD-75 was observed at 0.875% EMS and 0.5%EMS. respectively. Number of shoot, and percentage of rooting culture were decreasing as EMS level concentration increases.

ANALISIS KERAGAMAN GENETIK KENTANG HITAM Yulita, Kusumadewi Sri; Ahmad, Fajarudin; Martanti, Diyah; Poerba, Yuyu S.; Herlina, Herlina
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.

Co-Authors ,, Witjaksono Awang Maharijaya Eka Fatmawati Tihurua FAJARUDIN AHMAD Herlina Herlina Imelda, Maria IMELDA, MARIA KUSUMADEWI SRI YULITA Leksonowati, Aryani Prawestri, Apriliana Dyah S. Poerba, Yuyu Sobir Sobir Tri Handayani Witjaksono Witjaksono Witjaksono Wulansari, Aida Yudiwanti Wahyu E. Kusumo Yuyu S Poerba Yuyu S. Poerba, Yuyu S. Yuyu Suryasari Poerba

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