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Preparation of boron-doped diamond microelectrodes to determine the distribution size of platinum nanoparticles using current transient method Aliyah; Nurhidayat , Reza Rizqi; Afiten Rahmin Sanjaya; Rahmat Wibowo; Einaga, Yasuaki; Saepudin, Endang; Ivandini, Tribidasari Anggraningrum
Environmental and Materials Vol. 1 No. 1: (June) 2023
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/eam.v1i1.2023.117

Abstract

Boron-doped diamond (BDD) microelectrodes were prepared to investigate the correlation of hydrazine oxidation current responses with Pt nanoparticle (Pt NP) size distribution. The BDD film was grown on the surface of a tungsten needle with a diameter of 25 µm. An average particle size of around 5 µm BDD crystalline was successfully synthesized using a microwave plasma-assisted chemical vapor deposition technique. The Raman spectrum confirmed the presence of diamond formation as indicated by peaks corresponding to C-C sp3 bonds, while X-ray photoelectron spectroscopy spectrum showed the presence of C-H and C-OH bonds on the surface of the BDD microelectrode. Meanwhile the Pt nanoparticles was synthesized through reduction reaction of  PtCl62- solution using NaBH4 with citric acid as the capping agent. Particles size between 4.46 to 4.78 nm were observed by using TEM measurements. The BDD microelectrodes were utilized to investigate the relationship between Pt nanoparticle size distribution and the current generated from the oxidation reaction of 15 mM hydrazine in a 50 mM phosphate buffer solution pH 7.4 in the presence of 1.0 mL nanoparticle solutions. A current range of 5 and 6 nA with a noise level of 0.15 nA was observed showing a good correlation with the particle sizes of Pt NPs. Comparison was also performed with the measurements using Au microelectrodes, indicated that the prepared BDD microelectrodes is promising for the measurements of nanoparticle sizes distribution, especially Pt NPs.
UTILIZATION OF FISH PROTEIN HYDROLYSATE AS A NITROGEN SOURCE IN FERMENTED MEDIA OF CEPHALOSPORIN C PRODUCTION BY ACREMONIUM CHRYSOGENUM Biomcc 00141 Fatonah, Ani Fatonah; Saepudin, Endang; Suyanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8237

Abstract

Optimizing Indonesia's maritime resources as an effort to reduce reliance on imported raw materials for the pharmaceutical industry. This study investigates the use of fish protein hydrolysate, derived from trash fish flour, as a supplement in the fermentation process for producing cephalosporin C. This key raw material is used to create cephalosporin antibiotics through fermentation with the fungus Acremonium chrysogenum Biomcc 0141. Hydrolysate from trash fish flour is obtained through an enzymatic hydrolysis process using a protease found in pineapple hump extract which was optimized. The results of hydrolysis optimization on hydrolysate from trash fish flour produce a protein content of 3.37%. The protein hydrolysate obtained is then used in the cephalosporin C fermentation process. The addition of trash fish protein hydrolysate to cephalosporin C fermentation media showed a significant increase in the productivity of cephalosporin C, which is up to 85.39% higher than the fermentation process without the addition of fish protein hydrolysates.
PELATIHAN PEMOGRAMAN PLC PADA SMKN 1 DI CILEUNGSI Darwin, Darwin; Syaprudin, Syaprudin; Saepudin, Endang
Mitra Akademia: Jurnal Pengabdian Masyarakat Vol 1 No 2 (2018): Mitra Akademia: Jurnal Pengabdian Masyarakat
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat (P3M) Politeknik Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32722/mapnj.v1i2.1353

Abstract

Kegiatan Pengabdian Kepada Masyarakat sebagai salah satu Tri Dharma Perguruan Tinggi ini bertujuan tersedianya perangkat praktik pemograman PLC dan sarana penunjang lainnya di Sekolah Menengah Kejuruan Negeri 1 Cileungsi Bogor. Salah satu program keahlian yang terdapat di sekolah ini adalah Program Keahlian Otomasi. Tujuan Kompetensi Keahlian teknik Otomasi secara umum mengacu pada isi undangUndang Sistem Pendidikan Nasional (UU SPN) pasal 3 mengenai Tujuan Pendidikan Nasional dan penjelasan pasal 15 yang menyebutkan bahwa  pendidikan  kejuruan  merupakan  pendidikan  menengah  yang mempersiapkan peserta didik terutama untuk bekerja dalam bidang tertentu. Dengan tersedianya sarana praktik Pemograman PLC di SMK Negeri 1 Cileungsi, banyak manfaat yang dapat diperoleh terutama dalam meningkatkan kompetensi siswa Program Keahlian Teknik Otomasi. Diantara kompetensi dasar adalah Pemograman PLC. Dengan adanya pelatihan ini diharapkan siswa dapat mempraktikkan merancang bangun teknik Otomasi dengan menggunakan PLC sehingga dapat menjadi warga negara yang kompetitif, produktif, adaptif, selektif, dan kreatif. Human   Machine   Interface   (HMI)   merupakan   perangkat   antarmuka   antar pengguna dengan sistem kendali. Dengan perangkat ini suatu sistem dapat dimonitor, diatur, dan diambil datanya sesuai kebutuhan pengguna.  Pada Pengabdian Masyarakat ini dibuatlah sebuah modul latih PLC  dan HMI yang menggunakan   perangkat   HMI   Touchscreen Weintek MT8070IH5.  HMI Touchscreen berfungsi untuk memonitor, mengoperasikan dan mengontrol mesin atau proses di suatu plant dengan layar sentuh. Pada perangkat tersebut diatur sejalan dengan Proggramable Logic Controller (PLC) sehingga menghasilkan suatu sistem yang dapat dimonitor dan diatur secara baik. Untuk membuat perangkat ini bekerja secara bersama-sama perlu dilakukan pengaturan komunikasi pada alat dan dihubungkan oleh Ethernet.
Implementasi Solar Charging Controller Pada Penggerak Sirkulator Air Kolam Ikan di Kampung Setaman, Kota Depok Nuralam, Nuralam; Auditia, Ihsan; Purwanti, B. S. R.; Saepudin, Endang; Lestari K., Sri; Adiwinata, Syan Rosyid
Mitra Akademia: Jurnal Pengabdian Masyarakat Vol 7 No 2 (2024): Mitra Akademia: Jurnal Pengabdian Masyarakat
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat (P3M) Politeknik Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32722/mapnj.v7i2.6477

Abstract

The community needs-based community service activity of the Industrial Electronics Study Program, Department of Electrical Engineering (JTE) in Kampung Setaman, is an effort to solve the problems of the residents starting from the Covid-19-2020 period, where the residents of Kampung Setaman have around 100 pools, currently the remaining ones have decreased. In the 80s, most homeowners objected to paying for electricity to drive pump motors in fish ponds. As a result, some of the fish ponds died due to inactivity, ironically fish became a source of protein for the residents. Based on this, a solution emerged to create a Solar Power Generation System (PLTS). This is to provide solutions to residents' problems, so that the burden of electricity payments is reduced. Based on the objections to the electricity payment costs that emerged, the design of the PLTS included planning and empowering the pool as a healthy facility and business plan. This PLTS design refers to the concept of electricity distribution from one solar cell house (consisting of 4 cells) to 8 battery units. The aim of this PPIBM is to build a small-scale PLTS of 100 WP (400 WP), with the target of being able to activate 8 units of DC water pump motors for fish pond water circulation. The indicator of PPIBM's success is ensuring the process of storing unused electrical energy from solar cells for each battery. The solar cells are installed on a holo stand at a certain angle according to the direction of the sun, the position can be adjusted. Technical considerations get maximum solar intensity and excess energy can be stored in batteries. PLTS installation refers to the solar cell manual book, the service provider's contribution to the electronic system is so that the electrical energy activates the motor and some of it is stored in the battery (30%). The performance of the PLTS electrical system activates 8 motors during the day, 3 batteries supply electricity at night. The results of this installation are 4260 W (100 Ah) of power.
PENURUNAN TINGKAT KEMATIAN IKAN GURAMI MELALUI PENURUNAN KEKERUHAN AIR KOLAM DENGAN PROSES ELEKTROKOAGULASI   Widjajanto, Danang; Saepudin, Endang
Jurnal Pepadu Vol 6 No 3 (2025): Jurnal Pepadu
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v6i3.7928

Abstract

The turbidity of the water used for cultivating gourami fish by the Mina Lestari Fish Farmers group reaches 68 NTU and oxygen solubility is about 2.5 mg/L, while the maximum turbidity permitted for fish farming is 50 NTU and oxygen solubility more than 5 mg/L. Insufficient oxygen can cause the death of fish. To reduce water turbidity can be treating water using electrocoagulation. This community service activity aims to implement aelectrocoagulation process to reduce water turbidity, thereby increasing dissolved oxygen levels and reducing gourami mortality in RW 05, Duren Mekar, Depok. The methods used include site surveys, problem identification, partner coordination, program outreach, water reservoir for electrocoagulation process construction, coagulant producer construction, elctrocoagulation processing and evaluation. The electrocoagulation water reservoir for electrocoagulation process is made from concrete with 3m long, 1.5 m wide, and 1.2 m high. It is equipped with one 4-inch PVC pipe for the inlet and three 3-inch PVC pipes for the outlet. The electrocoagulation process is carried out by flowing 5 liters per minute of coagulant from coagulant storage to the water in reservoir. The electrocoagulation process was able to reduce water turbidity from 68 NTU to 43 NTU and increase dissolved oxygen from 2.5 mg/L to 5.6 mg/L as well as reduce the gourami fish deaths from 20 to 5 from a cultivation of 100 fish for 5 months. The conclusion is that the electrocoagulation process of pond water is able to produce water with turbidity that meets the requirements for cultivating gourami fish.
Biodegradation Potential of Cyanide and Nitrile using Bacteria of The Genus Rhodococcus Afianisa, Salma; Saepudin, Endang; Sunarko, Bambang; Sulistinah, Nunik
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v9i6.16689

Abstract

Cyanides and nitriles, characterized by their R-CN chains, are known for their toxicity, mutagenicity, and carcinogenicity, posing significant threats to environmental and human health. This study aims to explore the biodegradation capabilities of Rhodococcus sp. in breaking down cyanide and nitrile bonds. Rhodococcus pyridinivorans strain I-benzo was isolated from tanning waste and cultured in mineral media with a 20 mM benzonitrile substrate. The activity of this strain was tested using substrates such as benzonitrile, acetonitrile, acrylonitrile, benzamide, acetamide, and acrylamide, revealing positive reactions of nitrile hydratase and amidase enzymes through the Nessler measurement method, which indicated the production of ammonia and carboxylic acids. Furthermore, the degradation tests showed that the Vmax values for the biodegradation of potassium cyanide and sodium cyanide were 0.56 ppm/minute and 0.21 ppm/minute, respectively. These findings highlight the potential application of Rhodococcus pyridinivorans strain I-benzo in mitigating the environmental impact of cyanide and nitrile pollutants through efficient biodegradation.
UTILIZATION OF FISH PROTEIN HYDROLYSATE AS A NITROGEN SOURCE IN FERMENTED MEDIA OF CEPHALOSPORIN C PRODUCTION BY ACREMONIUM CHRYSOGENUM Biomcc 00141 Fatonah, Ani Fatonah; Saepudin, Endang; Suyanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8237

Abstract

Optimizing Indonesia's maritime resources as an effort to reduce reliance on imported raw materials for the pharmaceutical industry. This study investigates the use of fish protein hydrolysate, derived from trash fish flour, as a supplement in the fermentation process for producing cephalosporin C. This key raw material is used to create cephalosporin antibiotics through fermentation with the fungus Acremonium chrysogenum Biomcc 0141. Hydrolysate from trash fish flour is obtained through an enzymatic hydrolysis process using a protease found in pineapple hump extract which was optimized. The results of hydrolysis optimization on hydrolysate from trash fish flour produce a protein content of 3.37%. The protein hydrolysate obtained is then used in the cephalosporin C fermentation process. The addition of trash fish protein hydrolysate to cephalosporin C fermentation media showed a significant increase in the productivity of cephalosporin C, which is up to 85.39% higher than the fermentation process without the addition of fish protein hydrolysates.