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Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS) Ni Ketut Suwiti; I Wayan Masa Tenaya; I Nengah Kerta Besung
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this research was to compare the levels of growth hormone in bali cattle reared in the purification of Nusa Penida with those from other regions in Bali Province. A total of 320 sera samples were collected from bali cattle reared in Nusa Penida, Klungkung, Tabanan, Buleleng and Bangli. The levels of growth hormone was determined by using sandwich Enzime Linked Immunosorbent Assay (ELISA). The results showed that a diverse levels of growth hormone in Bali cattle were observed in Bali cattle. The lowest growth hormone levels (576.4 pg/mL) was found in Bali cattle reared in Nusa Penida and the highest levels (5044.08 pg/mL) were found in the Buleleng regency. It was concluded that bovine growth hormone levels varies, depending on the regions of rared of Bali cattle. ABSTRAK Penelitian ini bertujuan untuk mendeteksi kadar hormon pertumbuhan sapi bali yang dipelihara di wilayah pemurnian Nusa Penida dan wilayah lainnya di Provinsi Bali. Sebanyak 320 sampel serum sapi bali betina dewasa dikumpulkan dari peternakan rakyat di Nusa Penida Klungkung, Tabanan, Buleleng, dan Kabupaten Bangli. Kadar hormon pertumbuhan dideteksi dengan metode Enzime Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan, kadar hormon pertumbuhan sapi bali terendah (576,4 pg/mL) ditemukan pada sapi bali yang dipelihara di Nusa Penida, sedangkan kadar hormon tertinggi (5044,08 pg/mL) ditemukan pada sapi bali yang dipelihara di Kabupaten Buleleng. Disimpulkan bahwa kadar hormon pertumbuhan sapi bali berbeda-beda, tergantung wilayah tempat pemeliharaan sapi bali.

Kualitas Kimiawi dan Orgnanoleptik Daging Sapi Bali yang di Injeksi Growth Promotor Ni Wayan Ayu Rukmini; Ida Bagus Ngurah Swacita; Ni Ketut Suwiti; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 14 No. 4 August 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Telah dilakukan penelitian pengaruh pemberian growth promotor terhadap kualitas kimiawi dan organoleptik daging sapi bali yang diberkan perlakuan penyuntikan growth promotor. Penelitian eksperimental ini menggunakan Rancangan Acak Lengkap dengan dua perlakuan yaitu sapi yang diberi growth promotor dan sapi kontrol (tanpa diberi growth promotor) serta dua jenis daging yaitu sirloin dan shank. Penelitian diulang sebanyak enam kali. Sebanyak 24 sampel yang berasal dari 12 ekor sapi bali jantan umur 18 bulan yang diberi growth promotor dan tanpa diberi growth promotor. Pengujian dilakukan terhadap kualitas kimia (kadar protein, lemak, kadar air) dan organoleptik (warna, aroma, tekstur dan konsistensi). Hasil penelitian menunjukkan, injeksi growth promotor meningkatkan secara nyata kadar lemak dan protein kasar sedangkan kadar air daging sirloin dan shank menurun. Injeksi growth promotor memberikan warna dan tekstur yang lebih baik, namun tidak berpengaruh terhadap konsistensi dan aroma daging sapi bali.

Angka Lempeng Total Bakteri dan pH pada Cairan Rumen Sapi Bali Jantan yang Dipotong di Rumah Pemotongan Hewan Pesanggaran Ni Putu Nicky Mirahsanti; I Gusti Ketut Suarjana; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p01

Tujuan penelitian ini adalah untuk mengetahui pH dan angka lempeng total bakteri (ALTB) pada rumen sapi bali dengan materi penelitian berupa cairan rumen sapi bali jantan sebanyak 17 sampel. Penelitian ini merupakan penelitian observasional analitik pendekatan Cross Sectional dengan pengamatan terhadap angka lempeng total bakteri (ALTB) dan pH di dalam rumen. Data yang diperoleh selanjutnya disajikan secara deskriptif. Kemudian, perbedaan ALTB pada pH tertentu dianalisis dengan Independent Sample T- test. Hasil penelitian menunjukkan bahwa kisaran pH rumen sapi bali adalah 6,8 sampai 7 dengan rerata 6,4 ± 0,1 dan kisaran ALTB yaitu 32 x 103 sampai 171 x 103 CFU/ml dengan rerata 74 x 103 ± 47 x 103 CFU/ml. Tidak ada perbedaan yang signifikan antara ALTB pada pH 6,8 dan ALTB pada pH 7.

Respon Imun Terhadap Escherichia coli Pada Anak Babi yang Diberi Vaksin Rekombinan Escherichia coli – Avian Influenza Pieter Mbolo Maranata; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p15

Escherichia coli as a cause of colibacillosis is an important disease in pig farms because it causes weight loss, death in pigs, and significant economic losses. Handling colibacillosis by using antibiotics can cause a resistance effect, so we need an alternative to using a vaccine. The latest vaccine development by Udayana University using E. coli isolates from the Bali area was carried out to use this problem. Research on immunization against E. coli in piglets who have been given a recombinant vaccine E. coli - Avian Influenza. The sample used in this study was serum from 12 piglets. In this study, the sample was divided into two groups, namely the treatment group with vaccines and the control group without vaccine assistance. The group was given the vaccine at age of 1 week and repeated at age of 3 weeks, and the serum from both of groups was taken when the pigs were 4 weeks old. The serum is then tested using an Enzyme-Linked Immunosorbent Assay (ELISA) serological test. The results showed a varying optical density figures, the study group had an average optical density of 1.010 and the control group had an average of 0.418. Analysis statistic using the unpaired t-test, the two groups had significant differences. The level of antibodies against E. coli in vaccinated piglets is higher than the level of antibodies against E. coli in unvaccinated piglets. Diarrhea case data is used as supporting data that shows significant differences in the group given the booster vaccine in the third week. In the fourth week of the vaccination group, no piglets were diarrhea, while in the unvaccinated group 4 diarrhea was taken. The conclusion that were an increase in the immune response in pig that given the recombinant vaccine E. coli rather than control group.

Jumlah Khamir pada Rumen Sapi Bali Ditinjau dari pH dan Bobot Badan Ike Siwi Widyaningtyas; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p03

This study aims to determine the amount of yeast, rumen pH, and body weight of bali cattle. 17 samples were taken from the rumen fluid of bali cattle. This study was an analytic observational study with a cross sectional approach with observations on the number of yeast on rumen pH and body weight of bali cattle. The data obtained are then presented descriptively followed by regression correlation analysis. The results showed the average number of yeast in the rumen of bali cattle 21x103 ± 6.9x103 cells/ml, the average pH of rumen fluid of bali cattle 6.9 ± 0.07, and the average body weight of bali cattle 251 ± 47.8 kg. There is a significant difference between the number of yeast with the pH and body weight of bali cattle.

Faktor yang Mempengaruhi Perilaku dan Persepsi Dokter Hewan Bali Terhadap Penggunaan Antimikroba dan Resistensi Antimikroba Vera Paulina Sitanggang; I Nngah Kerta Besung; Hapsari Mahatmi
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p13

Antimicrobial resistance (AMR) is the ability of bacteria to withstand the effects of drugs so that bacteria do not die after the administration of antimicrobials and the function of the drug does not work at all. This incident is caused by the uncontrolled use of antimicrobials which can cause a global crisis on human and animal health in the future. This study aims to determine the factors that influence the behavior and perceptions of veterinarians in the province of Bali on the use of antimicrobials and antimicrobial resistance. A total of 204 veterinarians were used as research samples through a survey using a Likert system and then the results of differences in veterinary responses to the frequency of prescribing were analyzed by Kruskal-Wallis. The results showed that antimicrobials were given to half of the patients treated weekly, and the factors that influenced the administration of antimicrobials were the history of antimicrobial use, level of safety, experience, potency, side effects, and the risk of increasing antimicrobial resistance. The cost of culture testing and the price of antimicrobials are the biggest barriers for small animal veterinarians but not for livestock veterinarians. All respondents acknowledged that most sources of information on AMR were easy to obtain and useful for them. The group of veterinarians who practice small animals and livestock practitioners agree that AMR is a serious common threat in the future. This study provides important insights into the application of veterinary medical procedures in the province of Bali and will later become a reference in increasing regulation control over the use of antimicrobials.

Titer Antibodi Sekunder Terhadap Vaksin Streptococcus suis dengan Adjuvant Berbeda pada Tikus Putih Sumanna Rumapea; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p10

Streptococcus suis is a pathogenic bacteria which can cause an infection in animals nor humans. This study aimed to determine the difference in secondary antibody from the administration of S. suis vaccine with different adjuvants. This research is experimental with complete randomized design. Total of 15 rats was divided into 3 groups. Each groups was given S. suis vaccine with adjuvant MontanideTM ISA 78; MontanideTM ISA 201; MontanideTM Gel 01 by IM. The serum were taken from sinus orbitalis on the week 0 (pravacination), 1st week, 2nd week and 3rd week after booster. The secondary antibody titer were measured by indirect ELISA method. Data were analyzed using analysis of variance, then continued by LSD test. The result showed that S. suis vaccine with different adjuvants had a significant effect (P<0,05). S. S. suis vaccine with MontanideTM ISA 78 (w/o) produced the highest secondary antibody (OD = 0,331), then S. suis vaccine with MontanideTM Gel 01 (OD = 0,256). The lowest titer antibody was produced by S. suis vaccine with MontanideTM ISA 201 (w/o/w) adjuvant (OD = 0,208). From the results of this study, it can be concluded that the S.suis vaccine formulation with MontanideTM ISA 78 (w/o) is the best vaccine formulation in production of secondary antibody.

Deteksi Residu Antibiotik pada Daging Ayam Broiler dan Itik Serta Tingkat Kesadaran Peternak di Wilayah Selemadeg Timur Tabanan Bali Fitri Indah Permatasari; I Nengah Kerta Besung; Hapsari Mahatmi
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p17

Public consumption of chicken meat, especially broiler and duck, continues to increase. Various efforts have been taken by farmers to increase their production by increasing the quantity of their livestock and improving livestock management. The government has banned the use of antibiotics under Law no. 41 of 2014. The existence of this law needs to be evaluated on livestock products produced. This study aims to determine the presence of antibiotic residues and also to determine the level of awareness of farmers to antibiotic residues in broiler chicken and duck meat in the Selemadeg Timur Tabanan of ??Bali. This research is a descriptive study by detecting samples of chicken and duck meat using bioassays at BBVet Denpasar and by giving questionnaires to farmer. The number of samples was 10 samples of chicken meat, 2 samples of duck meat, and 15 samples kuesioner. Based on the results of the bioassay test, it was found that all samples were negative for residues of tetracycline antibiotics, aminoglycosides, macrolides, and penicillin. In addition, based on the results of questionnaire data, the level of public awareness of the use of antibiotics and the dangers of antibiotic residues in meat is very high. So that chicken meat in Selemadeg Timur ??Tabanan Bali is still said to be safe for consumption. However, there is still a need for supervision from the government and veterinarians regarding the use of antibiotics so that the quality of livestock products is maintained.

Sejumlah Faktor yang Melandasi Persepsi dan Perilaku Dokter Hewan terhadap Resistansi Antimikrob dan Penggunaan Antimikrob Vera Paulina Sitanggang; I Gede Hendra Prasetya Wicaksana; I Nengah Kerta Besung; Hapsari Mahatmi
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.424

Antimicrobial resistance (AMR) is the ability of bacteria to withstand the effects of drugs so that bacteria do not die after the administration of antimicrobials and the function of the drug does not work at all. This incident is caused by the uncontrolled use of antimicrobials which can cause a global crisis on human and animal health in the future. This study aims to determine the factors that influence the behavior and perceptions of veterinarians in the province of Bali on the use of antimicrobials and antimicrobial resistance. A total of 204 veterinarians were used as research samples through a survey using a Likert system and then the results of differences in veterinary responses to the frequency of prescribing were analyzed by Kruskal-Wallis. The results showed that antimicrobials were given to half of the patients treated weekly, and the factors that influenced the administration of antimicrobials were the history of antimicrobial use, level of safety, experience, potency, side effects, and the risk of increasing antimicrobial resistance. The cost of culture testing and the price of antimicrobials are the biggest barriers for small animal veterinarians but not for livestock veterinarians. All respondents acknowledged that most sources of information on AMR were easy to obtain and useful for them. The group of veterinarians who practice small animals and livestock practitioners agree that AMR is a serious common threat in the future. This study provides important insights into the application of veterinary medical procedures in the province of Bali and will later become a reference in increasing regulation control over the use of antimicrobials.

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