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All Journal Hemera Zoa POROS TEKNIK The Indonesian Biomedical Journal Sari Pediatri Universa Medicina Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Al Qalam: Jurnal Ilmiah Keagamaan dan Kemasyarakatan Jurnal Kreativitas PKM Bioscientia Medicina : Journal of Biomedicine and Translational Research JURNAL KESEHATAN BHAKTI HUSADA Bioscientia Medicina : Journal of Biomedicine and Translational Research COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat eJournal Kedokteran Indonesia Makara Journal of Health Research

Ahmad Aulia

Department Of Histology, Faculty Of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia

Author-ID : 294050

Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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LOW VITAMIN B12 DIET INCREASES LIVER HOMOCYSTEINE LEVELS AND LEADS TO LIVER STEATOSIS IN RATS Sianipar, Imelda Rosalyn; Ujianti, Irena; Yolanda, Sophie; Jusuf, Ahmad Aulia; Kartinah, Neng Tine; Amani, Patwa; Murti, Krishna Aditya; Soeria Santoso, Dewi Irawati
Universa Medicina Vol 38, No 3 (2019)
Publisher : Faculty of Medicine, Trisakti University

Background Nonalcoholic fatty liver disease (NAFLD) is one of the most widespread chronic liver diseases, caused by the development of insulin resistance. One of the mechanisms involved is a disturbance in insulin signaling by certain toxic substances that interact with one of the proteins responsible for the insulin signaling pathway. Increased homocysteine level, upon disruption of the methionine pathway, is associated with insulin resistance. The aim of this study was to evaluate the effect of hyperhomocysteinemia and insulin resistance (HOMA-IR level) induced by dietary vitamin B12 restriction on liver steatosis. Methods A study of laboratory experimental design was conducted involving 18 male Sprague Dawley rats (age 36-40 weeks, BW 300-350 g), that were randomly divided into 3 groups: control, 8-week treatment, and 16-week treatment. Standard AIN-93 diet was administered to the control group, whereas rats in the treatment groups were fed vitamin B12 deficiency-AIN-93M. At the end of treatment, liver homocysteine levels were determined by ELISA, HOMA-IR values were calculated, and steatosis degree of the liver was determined histologically. Statistical analysis was performed using independent t-test. Results A significant increase in liver homocysteine levels was found between the control and both the 8- and 16-week treatment groups (p<0.001). HOMA-IR levels were significantly higher in both treatment groups compared to controls (p<0.001). The area of liver steatosis in both treatment groups was significantly larger than that of the control group (p<0.001). Conclusion Increased homocysteine levels due to dietary vitamin B12 deficiency induces liver steatosis due to insulin resistance in rats.

PCS-5 Nitric Oxide Induced Basal Cell Hyperplasia and Lamina Propria Elongation in Rat Gastroesophageal Junction Tena Djuartina; Bambang Pontjo Priosoeryanto; Ari Fahrial Syam; Ahmad Aulia; Tri Isyani Tungga Dewi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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NO (Nitric Oxide) is an inorganic compound composed of nitrogen and oxygen, NO is also produced in various places on various types of mammalian cells. NO as a radical compound is important in mediating physiological and pathological events in mammals including humans [1].GEJ (gastroesophageal junction) is a transition zone between the surface of esophagus which is covered by stratified squamous epithelium to the gastric mucosa which consists of simple columnar epithelium (z-line), where circular muscle of esophagus fuse with oblique muscle and lipid layer of the gaster. At the lower part of GEJ, there is the lower esophageal sphincter (LES) that not only allow food to move into stomach and works as an exit passage of the gas, but also inhibit reflux of any substances that potentially can cause harm to the esophagus [2].Petersson et al, found that chronic exposure to cytotoxic levels of NO can cause inflammation, intestinal metaplasia and neoplasia. Although it is known that gastric acid, pepsin and bile acids can cause adenocarcinoma of distal esophagus and GEJ, NO exposure and nitrosative stress role in this phenomenon is yet to be fully understood and further study is needed [3].The purpose of this was to identify and compare the histopathological changes occurring in GEJ in relation to administration of physiological concentration of nitrate dissolved in HCl and ascorbic acid. As such, the animal model used in this study can be used to study and represent the changes microscopically, because obtaining a full thickness biopsy from a human subject can be difficult to perform.

Efek Proteksi Ekstrak Air Tanaman Akar Kucing (Acalypha Indica Linn) Terhadap Perubahan Struktur Neuron Hipokampus Paska Hipoksia Serebri Nurhadi Ibrahim; Ahmad Aulia Jusuf; Lina Marlina
JURNAL KESEHATAN BHAKTI HUSADA Vol 1 No 01 (2015): Jurnal Kesehatan Bhakti Husada
Publisher : UP3M AKPER-AKBID BHAKTI HUSADA CIKARANG

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Latar Belakang : Beberapa penelitian telah membuktikan bahwa ekstrak air akar kucing memiliki kemampuan neuroterapi dan neuroprotektor baik secara in vivo maupun in vitro. Telah terbukti juga ekstrak akar kucing dapat mengatasi berbagai masalah kesehatan sebagai antidiuretik, anti emetik dan anti toksin. Akar kucing juga mengandung antioksidan sehingga pemberian ekstrak tanaman ini mampu mencegah kerusakan sel akibat hipoksia. Atas dasar penelitian – penelitian tersebut, akan dibuktikan bahwa pemberian ekstrak akar kucing prahipoksia akan mencegah kerusakan struktur neuron hipokampus . Tujuan : Menganalisis perubahan struktur neuron hipokampus yang mendapat ekstrak air tanaman akar kucing sebelum perlakuan hipoksia yang dilaksanakan selama 7 hari Metode : Lima kelompok tikus sprage dawley yang terdiri dari masing – masing 5 tikus dikelompokan menjadi kelompok kontrol negatif hanya diberi aqua, kontrol positif dengan diberikan vitamin B1 dan 3 kelompok yang diberikan ekstrak air akar kucing dengan dosis 300 mg/kgBB, 400 mg/kgBB dan 500 mg/kgBB selama 7 hari sebelum paparan hipoksia.Setelah perlakuan hipoksia diambil jaringan hipokampusnya dan dilihat struktur neuronnya dengan pewarnaan hematoksilin eosin dan sel yang masih normal dihitung dengan menggunakan optilab viewer dan image raster. Hasil : Ekstrak akar kucing dengan dosis 300 mg/kgBB, 400 mg/kgBB dan 500 mg/kgBB menunjukan struktur neuron dan jumlah sel neuron dengan kelompok kontrol negatif ( tanpa ekstrak akar kucing) secara signifikan di area CA3 dan lapisan dalam girus dentatus hipokampus (p= 0,000) Kesimpulan : Ekstrak akar kucing dengan dosis 300, 400 dan 500 mg/kgBB mempunyai efek protektif terhadap kerusakan neuron di hipokampus

Mitochondrial Adaptations of Obese Adult Rat Visceral Adipocytes which Differ in Nutritional Status during Childhood Lailan Safina Nasution; Ahmad Aulia; Sri Widia A. Jusman; Mohamad Sadikin
eJournal Kedokteran Indonesia Vol 9, No. 3 - Desember 2021
Publisher : Faculty of Medicine Universitas Indonesia

Mitochondria are highly dynamic organelles that can adapt to different environmental stimuli. This study aims to analyze differences in the function and biogenesis of mitochondria and anaerobic glycolysis activity between obese adult rats that were undernourished in childhood (LCD28) and those which were normal (SD28) or already fat (HFD28). Sprague-dawley rats were assigned groups and given diet treatments from December 2017 until August 2018 in the Laboratory Animal Management Unit, Faculty of Veterinary Medicine, Bogor Agricultural Institute. To observe mitochondrial function and biogenesis, MnSOD activity and PGC1α levels were analyzed using ELISA. To investigate the existence of anaerobic glycolysis, LDH activity was analyzed using a spectrophotometry method. The results showed that MnSOD activity and PGC1α levels of LCD28 increased compared to the other groups, signifying higher mitochondrial function and biogenesis. Meanwhile, no significant difference in LDH activity was found, signifying similar levels of anaerobic glycolysis.

CD34+ UCB stem cells attenuate TGF-β signaling and inhibit liver fibrosis: A new avenue for liver cirrhosis-carcinogenesis prevention Septiana, Wahyunia Likhayati; Antarianto, Radiana Dhewayani; Louisa, Melva; Jusuf, Ahmad Aulia; Barasila, Atikah Chalida; Pawitan, Jeanne Adiwinata; Fasha, Iqbal
Makara Journal of Health Research Vol. 24, No. 2
Publisher : UI Scholars Hub

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Background: The liver microenvironment plays a key role in liver fibrosis and carcinogenesis. This study aimed to fill the gap in knowledge on the interaction between hepatic stellate cells and endothelial progenitor cells with biomarkers of liver fibrosis and/or carcinogenesis, including Col1A1, TGF-β, and tenascin-C. Methods: CD34+ stem cells were isolated from umbilical-cord-blood mononuclear cells. 2D and 3D co-culture of CD34+ UCB SCs and LX2 was performed. The cells were incubated in a CO2 incubator for three days. Morphological observation, qRT-PCR of TGF-β1 and COL1A1, and immunocytochemistry of tenascin-C were performed. Results: CD34+ UCB SCs were viable in the 2D and 3D co-culture for 24 h. 3D co-culture of CD34+ UCB SCs and LX2 inhibited in vitro liver fibrosis by lowering Col 1A1 expression as compared to control. We observed lower TGF-β expression in 3D co-culture on days 1 and 2 followed by higher expression of TGF-β on day 3. 2D co-culture of CD34+ UCB SCs and LX2 showed a different level of COL1A1 and TGF- β expression compared with 3D co-culture. Spheroids from 2D co-culture of CD34+ UCB SCs and LX-2 showed immunoreactivity against tenascin-C. Conclusion: Interaction between LX-2 and CD34+ UCB SCs in 3D co-culture inhibits in vitro liver fibrosis. The viability of CD34+ UCB SCs is essential for attenuation of TGF-β signaling in LX-2.

Formulasi dan Karakterisasi Capsaicin Konsentrasi Tinggi dalam Pembawa Transfersom pada Sediaan Gel Lestari, Novi; Iskandarsyah, Iskandarsyah; Jusuf, Ahmad Aulia
Al Qalam: Jurnal Ilmiah Keagamaan dan Kemasyarakatan Vol. 18, No. 1 : Al Qalam (Januari 2024)
Publisher : Sekolah Tinggi Ilmu Al-Qur'an (STIQ) Amuntai Kalimantan Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35931/aq.v18i1.3025

Capsaicin topikal dengan konsentrasi tinggi (8%) efektif dalam pengobatan nyeri pada pasien Post-Herpetic Neuralgia (PHN) dan HIV-Associated Distal Sensory Polyneuropathy (HIV-DSP), namun penggunaan capsaicin ini masih menimbulkan reaksi yang tidak diinginkan pada tempat aplikasi. Banyak penelitian telah dilakukan untuk mengurangi efek samping yang terjadi karena pemakaian capsaicin. Penelitian ini bertujuan memformulasikan dan mengkarakterisasi capsaicin transfersom konsentrasi tinggi pada sediaan gel serta melihat penetrasinya ke dalam kulit dengan alat sel difusi Franz. Formula Capsaicin transfersom menggunakan campuran Phospholiphon 90G dan Tween 80 dengan rasio (90:10); (85:15); dan (80:20). Formula optimum transfersom dihasilkan oleh campuran fosfatidilkolin (Phospholiphon 90G®) dan Tween 80 (80:20) dengan ukuran partikel 183,03±25,95 nm, potential zeta -33,87±0,83 mV, dan efisiensi penjerapan 71,58+0,12 %. Formula terpilih Transfersom dibentuk ke dalam suatu sediaan gel kemudian dibandingkan dengan gel non-transfersom untuk melihat penetrasinya ke dalam kulit melalui uji sel difusi Franz. Selanjutnya, dianalisa menggunakan metode Spektrofotometri UV-Vis dengan panjang gelombang 281 nm. Berdasarkan uji sel difusi Franz, didapatkan jumlah kumulatif gel capsaicin transfersom sebesar 7572,19 ng/cm2 dan gel capsaicin non-transfersom sebesar 20326,66 ng/cm2. Nilai fluks Capsaicin transfersom adalah 329,12 ng.cm-2jam-1 and capsaicin non-transfersom adalah 760,08 ng.cm-2jam-1. Dapat disimpulkan bahwa komposisi Phospholiphon 90G® dan Tween 80 dengan rasio 80:20 menghasilkan formula optimum dari transfersom capsaicin konsentrasi tinggi. Gel capsaicin transfersom memiliki kemampuan penetrasi yang lebih lambat dibandingkan capsaicin non transfersom. Hasil ini diharapkan dapat mengurangi efek samping dari capsaicin yang bersifat iritan, namun, perlu dilakukan penelitian lebih lanjut terkait hal ini.

Pengendali pada Papan Skor Pertandingan Bulutangkis Menggunakan Smartphone Android Sarifudin, Sarifudin; Aulia, Ahmad; Hakim, Al Rasyid Maulana; Banifade, Fadlil Malik
POROS TEKNIK Vol 15 No 1 (2023)
Publisher : P3M Politeknik Negeri Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31961/porosteknik.v15i1.2601

Teknologi pada zaman sekarang sudah jauh lebih maju, berkembang, dan berbagai jenis teknologi telah menjadi bagian dari kehidupan manusia pada saat ini yang dapat membantu serta memudahkan kegiatan manusia. Tentunya teknologi juga bisa diterapkan dalam hal olahraga. Dengan alasan itulah maka untuk memberikan kemudahan dalam cabang olah raga maka dibuatlah projek rancang bangun papan skor panel p10. Papan skor yang mudah dilihat dan di kontrol menjadi hal terpenting dalam satu pertandingan olah raga. Pemain dan penonton akan mendapatan informasi yang jelas pada saat berlangsungnya pertandingan. Dengan menggunakan methode komunikasi bluetooth pada smartphone android untuk kendali papan skor, kemudian diproses arduino berfungsi sebagai basis atau otak dari projek ini, dan menggunakan panel P10 yang dibuat dengan dirangkai dari 12 panel yang dijadikan satu sehingga ukurannya cukup besar sebagai display atau peran utama pada papan skor yang menampilkan nama, skor, babak, jumlah kemenangan yang didapat.

Pengaruh Kombinasi Terapi Akupunktur dan Medikamentosa Terhadap Keberhasilan Terapi Nyeri Kanker pada Anak Tyas, Nike Estu Renaning; Jusuf, Ahmad Aulia; Andriastuti, Murti; Djaali, Wahyuningsih
Sari Pediatri Vol 26, No 4 (2024)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp26.4.2024.236-43

Latar belakang. Di Amerika Serikat, kanker anak mencakup 2% dari seluruh kasus kanker. Kemajuan protokol terapi dalam beberapa tahun terakhir telah meningkatkan prognosis pasien kanker anak secara signifikan, tetapi juga memunculkan masalah baru, seperti nyeri kanker. Nyeri yang tidak tertangani dapat menurunkan kualitas hidup, mengganggu tidur, meningkatkan sensitivitas nyeri, dan menyulitkan tindakan medis. Akupunktur terbukti efektif sebagai terapi tambahan bersama pengobatan farmakologis untuk mengatasi nyeri kanker, sekaligus mengurangi dosis analgetik dan efek sampingnya.Tujuan. Tujuan dari penelitian ini adalah untuk mengetahui penggunaan obat analgetik, perubahan skor Visual Analog Scale S intra kelompok, dan keberhasilan terapi pada kelompok akupunktur dan medikamentosa dan kelompok medikamentosa saja pada nyeri kanker anak. Metode. Penelitian ini menggunakan desain kohort retrospektif dengan mengambil data di Rumah Sakit Umum Pusat Nasional dr. Cipto Mangunkusumo. Populasi terjangkau penelitian ini adalah pasien anak dengan nyeri kanker yang dirawat di Gedung Pusat Kesehatan Ibu Anak RSCM Kiara pada bulan Januari 2022- Juli 2023. Hasil. Kedua kelompok dapat menurunkan skor VAS dan terdapat beda signifikan. Akupunktur dan medikamentosa mempunyai peluang untuk dapat mengurangi penggunaan jenis obat analgetik, penggunaan ekstra obat dan pengurangan dosis total morfin harian, namun diperlukan penelitian lebih lanjut. Kedua kelompok memberikan hasil yang baik pada luaran keberhasilan terapiKesimpulan. Akupunktur dan medikamentosa mempunyai peluang untuk dapat mengurangi obat analgetik, mengurangi skor VAS, dan memberikan hasil yang baik untuk keberhasilan terapi, namun diperlukan penelitian lebih lanjut.

Histodynamics of Natural Killer Cells from a Healthy Donor Exposed to Exosomes from the Blood of Hepatocellular Carcinoma Patients Asrinda, Indria; Antarianto, Radiana Dhewayani; Jusuf, Ahmad Aulia; Ahani, Ardhi Rahman; Jasirwan, Chyntia Olivia Maurine; Ritchie, Ni Ken; Nur Aditya, Robby; Irawan, Cosphiadi
Makara Journal of Health Research Vol. 28, No. 3
Publisher : UI Scholars Hub

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Background: Hepatocellular carcinoma (HCC) is the leading form of liver cancer and the second leading cause of cancer-related deaths globally. Exosomes in the HCC microenvironment can induce significant changes in natural killer (NK) cells during endocytosis. The present study aimed to distinguish exosomes in the blood of HCC patients, analyze changes in NK cell phenotype, and evaluate peroxidase and toluidine blue staining as alternative methods for observing the changes. Methods: NK cells were collected from healthy donors, and exosomes were extracted from the blood of HCC patients. The exosomes were characterized in accordance with MISEV 2018 guidelines, and NK cells were incubated with HCC-derived exosomes. NK cell phenotype changes were assessed using immunofluorescence, toluidine blue staining, and peroxidase staining. Results: The identified exosomes measured 34.7 nm, had a charge of −4.33 mV, and were positive for CD81+. Changes in NK cell receptor expression following exposure to HCC exosomes were not significant (p > 0.05). Immunofluorescence confirmed exosome endocytosis by NK cells, toluidine blue staining revealed negative metachromasia and peroxidase staining indicated morphological NK cell changes. Conclusions: This study demonstrates that peroxidase and toluidine blue staining are effective for observing exosome endocytosis in NK cells, enhancing our understanding of HCC exosome-NK cell interactions and beneficial in developing future therapeutic strategies targeting the HCC microenvironment.

Thermally Oxidized Cooking Palm Oil-Induced Histopathological Alterations in Brain, Heart, Liver, and Kidney: A Systematic Review of Lipid Peroxidation and Inflammatory Mechanisms Nimas Ayu Nirwanti; Ahmad Aulia Jusuf
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 5 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i5.1273

Background: Repeated heating of cooking palm oils at high temperatures generates various toxic compounds, including lipid peroxidation products. These compounds are implicated in various diseases through oxidative stress and inflammation. This systematic review aims to evaluate the histopathological effects of thermally oxidized cooking oil (TOCO) consumption on the brain, heart, liver, and kidney, focusing on the roles of lipid peroxidation and inflammation. Methods: A systematic search was conducted in PubMed, Scopus, and Web of Science databases using predefined keywords and inclusion/exclusion criteria. Studies published between 2013 and 2024 investigating the histopathological effects of TOCO on the specified organs were included. Data on histopathological changes, markers of lipid peroxidation (malondialdehyde [MDA], 4-hydroxynonenal [4-HNE]), and inflammatory markers (TNF-α, IL-1β, IL-6) were extracted. Results: Seven studies met the inclusion criteria. The data revealed consistent histopathological changes across all four organs. In the brain, neuronal degeneration, astrogliosis, and microglial activation were observed. The heart exhibited cardiomyocyte hypertrophy, fibrosis, and inflammatory cell infiltration. The liver showed hepatocyte necrosis, steatosis, and inflammation. The kidneys presented with tubular necrosis, glomerular damage, and interstitial fibrosis. Elevated levels of MDA and 4-HNE were consistently reported in all affected tissues, along with increased expression of TNF-α, IL-1β, and IL-6. Conclusion: Consumption of TOCO induces significant histopathological damage in the brain, heart, liver, and kidney. The observed damage is strongly associated with increased lipid peroxidation and inflammatory responses. These findings highlight the potential health risks associated with consuming repeatedly heated cooking oils and underscore the need for public health awareness and strategies to mitigate these risks.

Co-Authors . Iskandarsyah Adiningsih Srilestari Ahani, Ardhi Rahman Amirulloh , Muhamad Antarianto, Radiana Dhewayani Ari Fahrial Syam Aria Kekalih Asrinda, Indria Bambang Pontjo Priosoeryanto Banifade, Fadlil Malik Barasila, Atikah Chalida Budi Wiweko Chandra, Inria Chyntia Olivia Maurine Jasirwan, Chyntia Olivia Maurine Cosphiadi Irawan Fasha, Iqbal Fitriani, Elies Goenarjo, Roman Hakim, Al Rasyid Maulana Hasan Mihardja Imelda Rosalyn Sianipar Irena Ujianti Jusman, Sri Widia Azraki Lailan Safina Nasution Lestari, Novi Lina Marlina Makkiyah, Feda Anisah Melva Louisa Mila Maidarti Mohamad Sadikin Murti Andriastuti, Murti Murti, Krishna Aditya Natalia, Zellah Fransisca Neng Tine Kartinah Almuktabar Ni Ken Ritchie, Ni Ken Nimas Ayu Nirwanti Nur Aditya, Robby Nurhadi Ibrahim Patwa Amani, Patwa Pawitan, Jeanne Adiwinata Puspita Eka Wuyung Sabita, Rifqi Salim Harris, Salim Santoso, Dewi Irawati Soeria Sarifudin sarifudin, Sarifudin Simadibrata, Christina Lanny Sophie Yolanda Sudaryat , Sudaryat Suratih, Ni Made Desy Tena Djuartina, Tena Tri Isyani Tungga Dewi Tyas, Nike Estu Renaning Viventius, Yoshua Wahyunia Likhayati Septiana Wahyuningsih Djaali Widyaprastuti, Hermin Yani, Sri

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