Garuda - Garba Rujukan Digital

Biodiversity of Endophytic Bacteria from Egg Plant in Lowland Purnawati, Arika; Nirwanto, Herry
Nusantara Science and Technology Proceedings 5th International Seminar of Research Month 2020
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0934

Endophytic bacteria are a group of microbes that live in plant tissues without causing negative effects and don’t cause sick on plants, are found in various plants, and can be isolated from all parts of the plant: roots, stems, and seeds. Endophytic bacteria in nature, have high diversity because in a single plant can be found more than one kind of endophytic bacteria, so many plants found many kinds of endophytic bacteria. Endophytic bacteria that have been isolated and identified from eggplants. This study aimed to isolate endophytic bacteria from eggplants in lowland Kandat Kediri. Crop sampling was conducted using a systematic random method. The research stages are surveys and microbial isolation of endophytes. Isolation is done from the stems of a healthy eggplant around the sick eggplant. The crop samples were then washed with running water and cut 1-3 cm. The cutting was sterilized by ethanol 70% (30 sec), sodium hypochlorite 5% (5 min), ethanol 70% (30 sec) and rinsed with sterile water (3 minutes). The results are from the stem get 10 isolates of endophytic bacteria that have different characteristics.

Effect of Formula Endophytic Bacteria in Organic Fertilizer to Wilt Disease Fusarium in Chili Plants Laras Setyowati; Arika Purnawati; Tri Mujoko
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3103

Chili (Capsicum annum) is one of the horticultural commodities that has an important role in human life and Indonesian chili plants have important economic value and occupy second place after beans. Meanwhile, in East Java, chili commodity production in 2013-2017 reached 440 tons and one of the highest contributors was Ringinsari, Kandat, Kediri. However, the increase in chili commodities in the village has so far been strongly influenced by the presence of the fungi pathogen is Fusarium oxysporum f.sp.capsici that cause wilt disease, can reduce production on average up to 75 % and cause the plant to die. The control of that pathogen generally uses chemical control that hurts humans and the environment so until now it have not been satisfactory. Depend of that it needs alternative control using endophytic bacteria that are formulated in organic fertilizer as biological control. Endophytic bacteria are group of bacteria that live in plant tissues without causing negative effects, including not causing plants to get sick and having a mutual relationship with their host plants. Endophytic bacteria can be found in various plants including agricultural crops and can be isolated from all parts of the plant, namely roots, stems, leaves, and seeds. Endophytic bacteria produce secondary metabolites and compounds that can be used to induce plant health and growth. Endophytic bacteria produce fenol, flavonoid that increase plant resistance to diseases and pests, improve plant health, phytohormones increase plant growth. The method stages of research are propagation of endophytic bacteria, manufacture of suspension of endophytic bacteria, mixing of suspension of endophytic bacteria into organic fertilizer, data analysis using analysis of variance and 5% BNT test. The results are Incubation period is slower, disease intensity decreases, and plant height increases.

Endophytic Bacteria from Wet Soil of South Kalimantan as Biological Control Agent for Root Nematodes (NPA) in Celery (Apium graveolens) Marcellino Althaf; Wilujeng Widayati; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3203

Endophytic bacteria are microorganisms that are associated with plants, don’t cause disease, and mutualism associated with plants. One of the uses of endophytic bacteria in agriculture is as biological agent in overcoming the attack of the root-knot nematode Meloidogyne sp. It is a polyphagous parasite that can attack plants by forming galls on the roots of celery plants (A. graveolens), can lack nutrients, and in chronic conditions causes death of host plants. It’s control is important to reduce crop yield loss. Control of the nematode Meloidogyne sp., at this time use many chemical pesticides because they quickly kill nematodes, but the continuous use of chemical nematicides will potentially pollute the environment. So, it is necessary to study alternative control using endophytic bacteria. The purpose of this study was to determine the potential of endophytic bacteria from wetlands of South Kalimantan as biological agents of it in celery plants. This research was conducted in Sumber Glagah, Pacet, Mojokerto, using Completely Randomized Design (CRD). The treatment was carried out by immersing the roots of celery plants in a suspension of endophytic bacteria (24 hours) and concentration (108 cfu/ml), then planted in polybags measuring 30 x 30. The polybags were filled with soil that had been divested with 50 juvenile Meloidogyne sp. Observation parameters were the number of galls per g of roots and juvenile population per g of root and per 10 g of soil. The results showed that the treatment of endophytic bacteria reduce: (1) number gall per g celery roots was 19.67–31.00 and control (46.67); (2) population juvenile population per g root was 9.33-25.67 and control 50.67; (3) juvenile population per 10 g of soil was 11.33-17.00 and control 29.33.

Natural Ingredients of Beans as Growth Medium and Propagation of Endophytic Bacteria from Lowland Eggplant Arika Purnawati; Safira Rizka Lestari; Iis Purnamawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3308

Endophytic bacteria are defined as a group of microbes that live in plant tissues that do not cause disease and are mutually related to the host plant, can be isolated from all parts of the plant were roots, stems, leaves, and seeds. Endophytic bacteria have the potential to produce natural products for agriculture, increase plant growth, and support the sustainable development of agriculture. The growth of endophytic bacteria in vitro generally uses a medium that contains nutrients including macronutrients, namely elements (C, H, O, and N), micronutrients, namely non-metallic elements (S and P), metallic elements (Ca, Zn, Na, K, Cu). , Mn, Mg, and Fe), vitamins, water, and energy. To meet these growth requirements, currently the growth and propagation of endophytic bacteria generally still use synthetic media which are expensive and in the field, mainly by farmers, are not easy to obtain. The study aimed to obtain an alternative medium made from beans for the growth and propagation of endophytic bacteria from lowland eggplants. The types of beans were soybeans, green beans, red beans, cowpeas, peanuts, and Nutrient Agar (NA) as control. Inoculation into the medium was done using the spread plate technique. This technique is preceded by a dilution and at dilution of 10-7 was taken 0.1 ml then to be spread on the growing medium. Observations were growing and colony counting was done 24 hours after inoculation. The results were: (1) endophytic bacteria from lowland eggplant can grow well on all mediums with the best growth on NA medium, (2) all media can be used as the propagation of endophytic bacteria from lowland eggplant plants, (3) the assumed of the growth curve is logarithmic with 4 phases were lag phase, exponential phase, stationer phase, death phase.

Formulation of Endophytic Bacteria in Organic Fertilizer as Health Promoter for Corn Plants in Mentaos, Gudo, Jombang Arika Purnawati; Tri Mujoko; Endang Triwahyu Prasetyawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3309

Corn (Zea mays) has an important role in the food supply in Indonesia, as the second commodity after rice and the second source of carbohydrates after rice which is a derivative of rice and is still considered feasible to meet national needs. The increase in national corn production is strongly supported by several corn production center provinces such as East Java and one of the districts that have the potential to support corn production in East Java is Jombang, but the reality the production experiences several obstacles, one of which is the presence of plant pest organisms (OPT). namely the fungus Peronosclerospora maydis caused by downy mildew. In the control of disease so far in the field, many use fungicides that are harmful to humans and the environment. The solution to reduce the use of fungicides is to utilize endophytic bacteria from eggplant and it has been formulated with organic fertilizer so that it is easier to apply to corn plants, this solution is expected to control disease rates, reduce losses, and improve corn plant health. The method used in community service in Mentaos, Gudo, and Jombang were situation analysis, problem identification, socialization that was attended by 25 farmers, and application of endophytic bacteria from eggplant plants in organic fertilizer formulas. The results obtained are that the people of Mentaos, Gudo, and Jombang were able to apply the endophytic bacterial formula in organic fertilizer with the sprinkle technique. The results show that the community can apply the endophytic bacterial formula, corn plants become healthy, and disease intensity is low.

Potential of Endophytic Fusarium sp. Against Bacterial Wilt Disease in Tomato Plants Nur Aini Mahfud; Ahmad Adibul Akrom; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4008

Ralstonia solanacearum is a pathogen that causes bacterial wilt in tomato plants (Lycopersicum esculentum), can decrease production until 90%. The controls generally use chemical pesticides which are harmful to the environment and humans, so it is necessary alternative controls using endophytic Fusarium sp. The aim of the research was to determine the effect of endophytic Fusarium sp. to bacterial wilt. Endophytic Fusarium sp. was explored on tomato plant in Tlekung, Junrejo-Batu and taken systematically randomly then brought to the laboratory. Isolation of endophytic fungi was done by washing the plants with running water, cutting 1cm then was sterilized. The sample was planted on Potato Dextrose Agar (PDA) and incubated at 37°C for 48 hours, if its growth was purified on new media. Isolation of R. solanacearum was done by washing the stems, cutting 0.5 cm then sterilizing. The piece is put into a test tube, added 10 ml of sterile water until the bacterial mass comes out and dissolves in sterile water. The mass of bacteria in sterile water is shaken using vortex until suspension forms. The suspension was grown on TZC agar and incubated at 280C for 24 hours, if it grow was purified on new media. Application of endophytic Fusarium sp. carried out using immersing technique of the roots of tomato plant. Inoculation of R. solanacearum was done into sterile soil before the plant were planted. The study used completely randomized design (CRD) with control and isolate of endophytic Fusarium sp. as treatments with 3 repeat and conducted in the greenhouse of the Faculty of Agriculture UPN "Veteran" East Java. Observations are incubation period and disease intensity, data were analyzed using BNT 5%. The results show that endophytic Fusarium sp. delayed the period incubation and suppressed the intensity of bacterial wilt disease in tomato plants.

The Effect of Water Activity on the Growth of Aspergillus flavus Laras Setyowati; Nijma Nurfadila; Arika Purnawati
Nusantara Science and Technology Proceedings The 4th International Conference on Agriculture and Environmental Sciences (ICAES) 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.3904

Aspergillus flavus is recognized as one of the fungi responsible for inducing diseases and causing damage to both food and feed commodities. This fungus is capable of producing mycotoxins that possess toxic attributes to both humans and animals. The contamination by A. flavus spans a broad spectrum, commencing from field cultivation and extending to storage facilities. One alternative approach to manage this fungus involves the modification of its growth environment. Microorganisms inherently possess a minimum water activity (aw) value crucial for their metabolic processes. The objective of this study is to modify the aw value to inhibit the growth of A. flavus. This research was conducted in vitro using PDA media supplemented with glycerol and distilled water to establish aw conditions of 0.90, 0.92, 0.95, and 0.97. On the seventh day after incubation, the results demonstrated that for 0.90 aw exhibited a significant inhibition on A. flavus growth, with an average colony diameter is 1.34 mm, followed by 0.92 is 1.54 then 0.95 is 1.83 mm, and 0.97 is 1.84 mm. Conversely, treatments with 0.90 displayed the lowest inhibitions (1.34 mm) and 0.97 displayed the highest (1.84 mm). All modified water activity treatments exerted an impact on the growth of A. flavus. As the aw value decreases, the growth of A. flavus becomes increasingly constrained.

Effect of Formula Endophytic Bacteria in Organic Fertilizer to Wilt Disease Fusarium in Chili Plants Laras Setyowati; Arika Purnawati; Tri Mujoko
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3103

Chili (Capsicum annum) is one of the horticultural commodities that has an important role in human life and Indonesian chili plants have important economic value and occupy second place after beans. Meanwhile, in East Java, chili commodity production in 2013-2017 reached 440 tons and one of the highest contributors was Ringinsari, Kandat, Kediri. However, the increase in chili commodities in the village has so far been strongly influenced by the presence of the fungi pathogen is Fusarium oxysporum f.sp.capsici that cause wilt disease, can reduce production on average up to 75 % and cause the plant to die. The control of that pathogen generally uses chemical control that hurts humans and the environment so until now it have not been satisfactory. Depend of that it needs alternative control using endophytic bacteria that are formulated in organic fertilizer as biological control. Endophytic bacteria are group of bacteria that live in plant tissues without causing negative effects, including not causing plants to get sick and having a mutual relationship with their host plants. Endophytic bacteria can be found in various plants including agricultural crops and can be isolated from all parts of the plant, namely roots, stems, leaves, and seeds. Endophytic bacteria produce secondary metabolites and compounds that can be used to induce plant health and growth. Endophytic bacteria produce fenol, flavonoid that increase plant resistance to diseases and pests, improve plant health, phytohormones increase plant growth. The method stages of research are propagation of endophytic bacteria, manufacture of suspension of endophytic bacteria, mixing of suspension of endophytic bacteria into organic fertilizer, data analysis using analysis of variance and 5% BNT test. The results are Incubation period is slower, disease intensity decreases, and plant height increases.

Endophytic Bacteria from Wet Soil of South Kalimantan as Biological Control Agent for Root Nematodes (NPA) in Celery (Apium graveolens) Marcellino Althaf; Wilujeng Widayati; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3203

Endophytic bacteria are microorganisms that are associated with plants, don’t cause disease, and mutualism associated with plants. One of the uses of endophytic bacteria in agriculture is as biological agent in overcoming the attack of the root-knot nematode Meloidogyne sp. It is a polyphagous parasite that can attack plants by forming galls on the roots of celery plants (A. graveolens), can lack nutrients, and in chronic conditions causes death of host plants. It’s control is important to reduce crop yield loss. Control of the nematode Meloidogyne sp., at this time use many chemical pesticides because they quickly kill nematodes, but the continuous use of chemical nematicides will potentially pollute the environment. So, it is necessary to study alternative control using endophytic bacteria. The purpose of this study was to determine the potential of endophytic bacteria from wetlands of South Kalimantan as biological agents of it in celery plants. This research was conducted in Sumber Glagah, Pacet, Mojokerto, using Completely Randomized Design (CRD). The treatment was carried out by immersing the roots of celery plants in a suspension of endophytic bacteria (24 hours) and concentration (108 cfu/ml), then planted in polybags measuring 30 x 30. The polybags were filled with soil that had been divested with 50 juvenile Meloidogyne sp. Observation parameters were the number of galls per g of roots and juvenile population per g of root and per 10 g of soil. The results showed that the treatment of endophytic bacteria reduce: (1) number gall per g celery roots was 19.67–31.00 and control (46.67); (2) population juvenile population per g root was 9.33-25.67 and control 50.67; (3) juvenile population per 10 g of soil was 11.33-17.00 and control 29.33.

Natural Ingredients of Beans as Growth Medium and Propagation of Endophytic Bacteria from Lowland Eggplant Arika Purnawati; Safira Rizka Lestari; Iis Purnamawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3308

Endophytic bacteria are defined as a group of microbes that live in plant tissues that do not cause disease and are mutually related to the host plant, can be isolated from all parts of the plant were roots, stems, leaves, and seeds. Endophytic bacteria have the potential to produce natural products for agriculture, increase plant growth, and support the sustainable development of agriculture. The growth of endophytic bacteria in vitro generally uses a medium that contains nutrients including macronutrients, namely elements (C, H, O, and N), micronutrients, namely non-metallic elements (S and P), metallic elements (Ca, Zn, Na, K, Cu). , Mn, Mg, and Fe), vitamins, water, and energy. To meet these growth requirements, currently the growth and propagation of endophytic bacteria generally still use synthetic media which are expensive and in the field, mainly by farmers, are not easy to obtain. The study aimed to obtain an alternative medium made from beans for the growth and propagation of endophytic bacteria from lowland eggplants. The types of beans were soybeans, green beans, red beans, cowpeas, peanuts, and Nutrient Agar (NA) as control. Inoculation into the medium was done using the spread plate technique. This technique is preceded by a dilution and at dilution of 10-7 was taken 0.1 ml then to be spread on the growing medium. Observations were growing and colony counting was done 24 hours after inoculation. The results were: (1) endophytic bacteria from lowland eggplant can grow well on all mediums with the best growth on NA medium, (2) all media can be used as the propagation of endophytic bacteria from lowland eggplant plants, (3) the assumed of the growth curve is logarithmic with 4 phases were lag phase, exponential phase, stationer phase, death phase.

Title

Found 11 Documents
Search
Journal : Nusantara Science and Technology Proceedings

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 11 Documents Search Journal : Nusantara Science and Technology Proceedings

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 11 Documents
Search
Journal : Nusantara Science and Technology Proceedings