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Biodiversity of Endophytic Bacteria from Egg Plant in Lowland Purnawati, Arika; Nirwanto, Herry
Nusantara Science and Technology Proceedings 5th International Seminar of Research Month 2020
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0934

Endophytic bacteria are a group of microbes that live in plant tissues without causing negative effects and don’t cause sick on plants, are found in various plants, and can be isolated from all parts of the plant: roots, stems, and seeds. Endophytic bacteria in nature, have high diversity because in a single plant can be found more than one kind of endophytic bacteria, so many plants found many kinds of endophytic bacteria. Endophytic bacteria that have been isolated and identified from eggplants. This study aimed to isolate endophytic bacteria from eggplants in lowland Kandat Kediri. Crop sampling was conducted using a systematic random method. The research stages are surveys and microbial isolation of endophytes. Isolation is done from the stems of a healthy eggplant around the sick eggplant. The crop samples were then washed with running water and cut 1-3 cm. The cutting was sterilized by ethanol 70% (30 sec), sodium hypochlorite 5% (5 min), ethanol 70% (30 sec) and rinsed with sterile water (3 minutes). The results are from the stem get 10 isolates of endophytic bacteria that have different characteristics.

Effect of Formula Endophytic Bacteria in Organic Fertilizer to Wilt Disease Fusarium in Chili Plants Laras Setyowati; Arika Purnawati; Tri Mujoko
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3103

Chili (Capsicum annum) is one of the horticultural commodities that has an important role in human life and Indonesian chili plants have important economic value and occupy second place after beans. Meanwhile, in East Java, chili commodity production in 2013-2017 reached 440 tons and one of the highest contributors was Ringinsari, Kandat, Kediri. However, the increase in chili commodities in the village has so far been strongly influenced by the presence of the fungi pathogen is Fusarium oxysporum f.sp.capsici that cause wilt disease, can reduce production on average up to 75 % and cause the plant to die. The control of that pathogen generally uses chemical control that hurts humans and the environment so until now it have not been satisfactory. Depend of that it needs alternative control using endophytic bacteria that are formulated in organic fertilizer as biological control. Endophytic bacteria are group of bacteria that live in plant tissues without causing negative effects, including not causing plants to get sick and having a mutual relationship with their host plants. Endophytic bacteria can be found in various plants including agricultural crops and can be isolated from all parts of the plant, namely roots, stems, leaves, and seeds. Endophytic bacteria produce secondary metabolites and compounds that can be used to induce plant health and growth. Endophytic bacteria produce fenol, flavonoid that increase plant resistance to diseases and pests, improve plant health, phytohormones increase plant growth. The method stages of research are propagation of endophytic bacteria, manufacture of suspension of endophytic bacteria, mixing of suspension of endophytic bacteria into organic fertilizer, data analysis using analysis of variance and 5% BNT test. The results are Incubation period is slower, disease intensity decreases, and plant height increases.

Endophytic Bacteria from Wet Soil of South Kalimantan as Biological Control Agent for Root Nematodes (NPA) in Celery (Apium graveolens) Marcellino Althaf; Wilujeng Widayati; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3203

Endophytic bacteria are microorganisms that are associated with plants, don’t cause disease, and mutualism associated with plants. One of the uses of endophytic bacteria in agriculture is as biological agent in overcoming the attack of the root-knot nematode Meloidogyne sp. It is a polyphagous parasite that can attack plants by forming galls on the roots of celery plants (A. graveolens), can lack nutrients, and in chronic conditions causes death of host plants. It’s control is important to reduce crop yield loss. Control of the nematode Meloidogyne sp., at this time use many chemical pesticides because they quickly kill nematodes, but the continuous use of chemical nematicides will potentially pollute the environment. So, it is necessary to study alternative control using endophytic bacteria. The purpose of this study was to determine the potential of endophytic bacteria from wetlands of South Kalimantan as biological agents of it in celery plants. This research was conducted in Sumber Glagah, Pacet, Mojokerto, using Completely Randomized Design (CRD). The treatment was carried out by immersing the roots of celery plants in a suspension of endophytic bacteria (24 hours) and concentration (108 cfu/ml), then planted in polybags measuring 30 x 30. The polybags were filled with soil that had been divested with 50 juvenile Meloidogyne sp. Observation parameters were the number of galls per g of roots and juvenile population per g of root and per 10 g of soil. The results showed that the treatment of endophytic bacteria reduce: (1) number gall per g celery roots was 19.67–31.00 and control (46.67); (2) population juvenile population per g root was 9.33-25.67 and control 50.67; (3) juvenile population per 10 g of soil was 11.33-17.00 and control 29.33.

Effect of Formula Endophytic Bacteria in Organic Fertilizer to Wilt Disease Fusarium in Chili Plants Laras Setyowati; Arika Purnawati; Tri Mujoko
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3103

Chili (Capsicum annum) is one of the horticultural commodities that has an important role in human life and Indonesian chili plants have important economic value and occupy second place after beans. Meanwhile, in East Java, chili commodity production in 2013-2017 reached 440 tons and one of the highest contributors was Ringinsari, Kandat, Kediri. However, the increase in chili commodities in the village has so far been strongly influenced by the presence of the fungi pathogen is Fusarium oxysporum f.sp.capsici that cause wilt disease, can reduce production on average up to 75 % and cause the plant to die. The control of that pathogen generally uses chemical control that hurts humans and the environment so until now it have not been satisfactory. Depend of that it needs alternative control using endophytic bacteria that are formulated in organic fertilizer as biological control. Endophytic bacteria are group of bacteria that live in plant tissues without causing negative effects, including not causing plants to get sick and having a mutual relationship with their host plants. Endophytic bacteria can be found in various plants including agricultural crops and can be isolated from all parts of the plant, namely roots, stems, leaves, and seeds. Endophytic bacteria produce secondary metabolites and compounds that can be used to induce plant health and growth. Endophytic bacteria produce fenol, flavonoid that increase plant resistance to diseases and pests, improve plant health, phytohormones increase plant growth. The method stages of research are propagation of endophytic bacteria, manufacture of suspension of endophytic bacteria, mixing of suspension of endophytic bacteria into organic fertilizer, data analysis using analysis of variance and 5% BNT test. The results are Incubation period is slower, disease intensity decreases, and plant height increases.

Endophytic Bacteria from Wet Soil of South Kalimantan as Biological Control Agent for Root Nematodes (NPA) in Celery (Apium graveolens) Marcellino Althaf; Wilujeng Widayati; Arika Purnawati
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3203

Endophytic bacteria are microorganisms that are associated with plants, don’t cause disease, and mutualism associated with plants. One of the uses of endophytic bacteria in agriculture is as biological agent in overcoming the attack of the root-knot nematode Meloidogyne sp. It is a polyphagous parasite that can attack plants by forming galls on the roots of celery plants (A. graveolens), can lack nutrients, and in chronic conditions causes death of host plants. It’s control is important to reduce crop yield loss. Control of the nematode Meloidogyne sp., at this time use many chemical pesticides because they quickly kill nematodes, but the continuous use of chemical nematicides will potentially pollute the environment. So, it is necessary to study alternative control using endophytic bacteria. The purpose of this study was to determine the potential of endophytic bacteria from wetlands of South Kalimantan as biological agents of it in celery plants. This research was conducted in Sumber Glagah, Pacet, Mojokerto, using Completely Randomized Design (CRD). The treatment was carried out by immersing the roots of celery plants in a suspension of endophytic bacteria (24 hours) and concentration (108 cfu/ml), then planted in polybags measuring 30 x 30. The polybags were filled with soil that had been divested with 50 juvenile Meloidogyne sp. Observation parameters were the number of galls per g of roots and juvenile population per g of root and per 10 g of soil. The results showed that the treatment of endophytic bacteria reduce: (1) number gall per g celery roots was 19.67–31.00 and control (46.67); (2) population juvenile population per g root was 9.33-25.67 and control 50.67; (3) juvenile population per 10 g of soil was 11.33-17.00 and control 29.33.

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