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All Journal Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) The Indonesian Biomedical Journal Jurnal Abdimas Kesehatan
Senny Yesery Esar
Faculty Of Pharmacy, Universitas Katolik Widya Mandala Surabaya, Jl. Kalisari Selatan 1, Surabaya 60237
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Nursing Public Health

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Deteksi populasi Cd3+Cd4+Cd25+Foxp3+ T-regulator pada Limpa Mencit Galur Balb/c dan Swiss-webster yang Mudah dan Cepat dengan Metode Flow Cytometry Yudy Tjahjono; Hendy Wijaya; Senny Yesery Esar; Caroline Caroline; Nico Jafet; I Made Andika Bara Kusuma; Oryza Chrisantia; Maria Theresia Primadewy Bhendy; Sindi Siska Palpialy; Shellin Soehadi; Yufita Ratnasari Wilianto
Jurnal Farmasi Sains dan Terapan Vol 9, No 2 (2022): October
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v9i2.4079

Abstract

Sel T-regulator (Treg) memainkan peran kunci dalam mengendalikan autoimunitas, respon alergi, peradangan, dan respons terhadap infeksi. Sel T Cd4+ yang secara konstitutif mengekspresikan Cd25 dan protein forkhead 3 (Foxp3; yaitu, Cd3+Cd4+Cd25+Foxp3+) atau disebut juga sebagai Treg fungsional, telah terbukti memainkan peran utama dalam pemeliharaan toleransi dan homeostasis imun, sehingga deteksi Treg fungsional sangat penting dilakukan sebagai salah satu parameter imunomodulasi. Penelitian ini bertujuan untuk mendeteksi populasi sel Treg (Cd3+Cd4+Cd25+Foxp3+) pada organ limpa mencit galur Balb/c dan Swiss-webster dengan menggunakan metode yang mudah, cepat, serta terjangkau. Metode deteksi sel Treg dilakukan dengan cara isolasi organ limpa mencit Balb/c dan Swiss-webster kemudian dilakukan preparasi dan deteksi sel menggunakan instrumen flow cytometry. Hasil populasi sel Treg yang didapatkan berturut-turut pada mencit Balb/c dan Swiss-webster adalah 2,02 ± 0,36% dan 3,14 ± 1,64% dari total populasi yang dideteksi dengan hanya membutuhkan waktu 145 menit sejak pembedahan. Berdasarkan hasil tersebut dapat disimpulkan bahwa pengamatan populasi sel Treg pada splenosit mencit galur Balb/c dan Swiss-webster dengan flow cytometry dapat dilakukan dengan mudah dan praktis dengan pewarnaan kombinasi antibodi yang tertera pada manuskrip ini. Selain itu, metode pewarnaan yang tertera dapat digunakan sebagai salah satu acuan untuk mendeteksi imunomodulator pada berbagai model hewan coba yang menggunakan mencit galur Balb/c dan Swiss-webster, khususnya yang berhubungan dengan populasi sel Treg.
2-(3-(chloromethyl)benzoyloxy)benzoic Acid Increases CD4+ Regulatory T-Cell Population and FoxP3 Expression in Lipopolysaccharide-induced Mice Yudy Tjahjono; Caroline Caroline; Jusak Nugraha; Kuncoro Foe; Srikanth Karnati; Süleyman Ergün; Nico Jafet; Oryza Chrisantia; I Made Andika Bara Kusuma; Hendy Wijaya; Wuryanto Hadinugroho; Dwi Aris Agung Nugrahaningsih; Dwi Liliek Kusindarta; Yufita Ratnasari Wilianto; Senny Yesery Esar; Hevi Wihadmadyatami
The Indonesian Biomedical Journal Vol 15, No 4 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i4.2429

Abstract

BACKGROUND: Lipopolysaccharide (LPS) has been reported to increase CD4+ regulatory T-cell (CD4+ Treg) populations. Acetylsalicylic acid (ASA) has been reported to have immunomodulatory activity, but it may induce chronic gastric ulceration. Another salicylic acid-bearing compound, 2-(3-(chloromethyl)benzoyloxy)benzoic acid (3-CH2Cl), has been reported to have less gastric mucosal damage. However, the effect of 3-CH2Cl on CD4+ Tregs in LPS-induced mice is still unknown. Therefore, the present study was conducted to investigate the immunomodulatory effect of 3-CH2Cl on CD4+ T-cell and CD4+ Treg populations as well as FoxP3 expression in LPS-induced mice.METHODS: Synthesis of 3-CH2Cl was performed by mixing salicylic acid and chloromethylbenzoylchloride with the catalyzation of pyridine, acetone and heat. The 3-CH2Cl tablets were prepared using direct compression method. After intraperitoneal injection of 1 mg/kg BW LPS to mice, 60 mg/kg BW ASA or 60 mg/kg BW 3-CH2Cl was given orally for 3 days. The splenocyte was obtained through splenectomy and collagenase digestion. The population of CD4+ T-cells and CD4+ Tregs, as well as the splenic FoxP3 expression were determined using flow cytometry technique.RESULTS: CD4+ T-cell populations in mice treated with LPS and 3-CH2Cl or ASA were lower than those treated with LPS merely. Meanwhile, CD4+ Treg populations and FoxP3 expression levels in mice treated with LPS and 3-CH2Cl or ASA were higher than those treated with LPS merely.CONCLUSION: Since 3-CH2Cl could decrease CD4+ T-cell population and increase CD4+ Treg population mediated by the increase of FoxP3 expression in LPS-induced inflammation, it may act as a potential therapeutic drug to reduce inflammatory conditions.KEYWORDS: 2-(3-(chloromethyl)benzoyloxy)benzoic acid, acetylsalicylic acid, CD4, T-regulatory cells, FoxP3, LPS
Layanan Telemedicine Terintegratif Dokter dan Apoteker bagi Penderita Covid-19 di Lingkungan Paroki Kristus Raja Surabaya Caroline Caroline; Senny Yesery Esar; Martha Ervina; Wuryanto Hadinugroho; Steven Steven; Yudhikuari Sincihu; Laura Wihanto
Jurnal Abdimas Kesehatan (JAK) Vol 4, No 3 (2022): November
Publisher : Sekolah Tinggi Ilmu Kesehatan Baiturrahim Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36565/jak.v4i3.388

Abstract

The global pandemic related to the coronavirus (2019-nCoV) continues to hit the world, including Indonesia until the end of 2021. The efforts to improve health services were carried out by various parties, including by members of the King of Christ Parish church as part of the Surabaya community. From this parish in July 2021, 301 people were sick with COVID-19 and almost 11% of them died. The public and health services were panicked by the occurrence of a wave of viral mutations, with the increasing of sufferers and high mortality rates. Integrated telemedicine services between doctors and pharmacists are one of the best options to prevent transmission and severity to support patient recovery rates. With telemedicine, health care needs are individualized and patient development is more controlled. The method was carried out through direct consultation between doctors, pharmacist, and patients, using the whatsapp call platform. Monitoring of patients was continued by pharmacist using the whatsapp message platform until the patient was cured. If necessary, consultation with the doctor could be carried out again during the patient’s healing process.  The research show that there is an integration of doctor and pharmacist services in telemedicine for COVID-19 patients in diagnosing, determining the best supporting drugs and therapies for the patients. Quantitatively, there was a decrease in the mortality rate, an increase in patient compliance and recovery. As an additional indicator, the residents welcome the integrated services that are carried out and can be developed for other health services.
Co-Authors Caroline Caroline Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Foe, Kuncoro Hendy Wijaya Hendy Wijaya Hevi Wihadmadyatami I Made Andika Bara Kusuma I Made Andika Bara Kusuma Jusak Nugraha Laura Wihanto Maria Theresia Primadewy Bhendy Martha Ervina Nico Jafet Nico Jafet Oryza Chrisantia Oryza Chrisantia Shellin Soehadi Sindi Siska Palpialy Srikanth Karnati Steven, . Süleyman Ergün Wuryanto Hadinugroho Wuryanto Hadinugroho Yudhikuari Sincihu Yudy Tjahjono Yudy Tjahjono Yufita Ratnasari Wilianto Yufita Ratnasari Wilianto