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All Journal Indonesian Journal of Tropical and Infectious Disease Journal Of Widya Medika Junior Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Jurnal Penyakit Dalam Indonesia Jurnal Abdimas Kesehatan Jurnal Widya Medika
Laura Wihanto
Department Of Microbiology And Parasitology, Faculty Of Medicine, Universitas Katolik Widya Mandala Surabaya, Surabaya, Indonesia
Earth & Planetary Sciences Health Professions Medicine & Pharmacology Nursing Public Health

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Antibacterial Effect Of Curcuma Domestica Ethanolic Extract On Group A Streptococcus Î’ Hemoyticus Halim, Stefan W; Wihanto, Laura; Sutandhio, Silvia
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 1, No 3 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (674.081 KB) | DOI: 10.33508/jwmj.v1i3.2098

Abstract

Introduction : Pharyngitis is one of the most common infection that general practitioners handled. It is estimated in one year there are 15 million people with pharyngitis will come to the doctor. American Society of Microbiology said that 94,3 % from 402 patient who were suspected pharyngitis received antibiotic therapy even without indication of antibiotic therapy. Antibiotic resistance is becoming a great threat for the world today. World Health Organization state that one of the plan to fight antibiotic resistance is to research and develop new drugs. Curcuma domestica is a plant that is widely used in Indonesia and some research said that it has anti-inflammatory, antioxidant, and antimicrobial effect. Aim: The aim of this study is to find the antibacterial effect of Curcuma domestica ethanolic extract on Group A Streptococcus ? hemolyticus and find the Minimum Bactericidal Concentration (MBC) value. Methods : This was in vitro experimental study with broth microdilution method and inoculation on agar blood media to find the MBC. Treatment group consisting of, media (Mueller Hinton Blood Broth), Group A Streptococcus ? hemolyticus bacteria, and 5 concentrations (312.5 ?g/ml, 625 ?g/ml, 1250 ?g/ml, 2500 ?g/ml, 5000 ?g/ml) of Curcuma domestica extract. Result : There is no bacterial growth at 5000 ?g/ml concentration on solid media, but there are less bacterial growth at 2500 ?g/ml than at concentration 312.5 ?g/ml, 625 ?g/ml, 1250 ?g/ml. Conclusion: There is an antibacterial effect of Curcuma domestica ethanolic extract on Group A Streptococcus ? hemolyticus with MBC value in range 2500 ? 5000 ?g/ml.
Fever Duration, Hepatomegaly And Overweight With The Dengue Shock Syndrome In Kupang, East Nusa Tenggara Cundawan, Robertus S; Wihanto, Laura; Wijono, Steven
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 4 (2020): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v2i4.2735

Abstract

Introduction: Dengue infection is an infection caused by the dengue virus. Community-widespread cases of morbidity and mortality are often found in Extraordinary Events (KLB)in Indonesia, especially in children. Purpose: The purpose of this study was to prove the correlation between the duration of fever, hepatomegaly, and overweight with dengue shock syndrome in Kupang, East Nusa Tenggara. Methods: This research is an observational analytic study with a case-control approach—the subject in this study was data of dengue infection patients aged 5-14 years. The research instrument used was the patient's medical record data for the period June 2018-June 2019. Bivariate analysis was done using chi-square statistical test. Results: The results showed that there was a significant correlation (p<0.05) between the duration of fever (OR=0,35;p=0,04), hepatomegaly (OR=3,34;p=0,022), and overweight (OR=2,88;p=0,033) with dengue shock syndrome in Kupang, East Nusa Tenggara. Conclusion: There is a significant relationship between the duration of fever, hepatomegaly, overweight with dengue shock syndrome.
Behavior of Eradicating Mosquito Nests and Larva Index With Incidence Rate of Dengue Infection at Kupang City de Fatima, Julio CA; Steven, Steven; Wihanto, Laura
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 2, No 3 (2020): July
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v2i3.2643

Abstract

Introduction : Dengue infection occurs at a large scale in Indonesia, especially in Kupang City. The incidence rate of dengue infection in January 2019 was 245 cases and caused 15 deaths. The number of cases and mortality at Kupang City increased in 2019. In 2018, dengue infection attacked 210 persons, and one person died due the infection. Thus Kupang City was determined to have extraordinary incidents of dengue infection by the government. Aim : This research was conducted to determine whether there is a relationship between larvae index and the behavior of eradicating mosquito nest with the incidence of dengue infection in the city of Kupang. Method: This study used a cross-sectional design and data collection was carried out by random sampling with a total sample of 60 samples divided into three working areas of the health center and secondary data in the form of the incidence of dengue infections obtained from the health center in January-June 2019 period. Results: there was a significant relationship between the behavior of eradicating mosquito nest with the incidence rate of dengue infection in Kupang City (p=0,006). A significant correlation between larva existence with the incidence rate of dengue infection (p=0,006) and significant correlation between the behavior of eradicating mosquito with larva existence (p=0,000). Conclusion: there is a significant relationship between the behavior of eradicating mosquito nests and larva existence with the incidence rate of dengue infection at Kupang City.
Efektivitas Ekstrak Strobilanthes crispus terhadap Kadar Glukosa Darah Puasa dan Pasca Tes Toleransi Glukosa Oral pada Rattus norvegicus Diabetes Melitus Gunawan, Alvin; Wihanto, Laura; Muliono, Ari Christy
Jurnal Penyakit Dalam Indonesia Vol. 10, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Introduction. The 2018 Riskesdas data shows that the prevalence of diabetes mellitus is increasing each year. Strobilanthes crispus leaves are known for lowering blood glucose levels. This study aimed to determine the difference in the effectiveness of S. crispus leaves ethanolic extract on fasting blood glucose (FBG) and post-oral glucose tolerance test (OGTT). Methods. True experimental study using post-test only control group design and Rattus norvegicus as experimental animals. Rattus norvegicus were selected by random sampling which were then divided into 4 groups (K, P1, P2, P3), each group consisted of 5 animals. The animals were subsequently induced with diabetes mellitus via a single injection of 160 mg/kgBW/IP alloxan. Group K was given 0.5% NaCMC solution at a dose of 1 ml/200 g BW/day/oral/day. Group P1, P2, and P3 received extract at a dose of 100, 400, 600 mg/kg BW/oral/day for 21 days using oral gavage. Statistical analysis was performed using ANOVA, Tukey, Brown-Forsythe, and Kruskal-Wallis tests. Results. Mean FBG level in group K, P1, P2, P3 were 338.40 mg/dL, 270.80 mg/dL, 200.00 mg/dL, and 138.60 mg/dL, respectively. Mean value of 60 minutes OGTT blood glucose level in group K, P1, P2, P3 were 355.60 mg/dL, 289.00 mg/dL, 188.00 mg/dL, and 164.40 mg/dL, respectively. Meanwhile, mean value of 120 minutes OGTT blood glucose level in group K, P1, P2, P3 were 346.60 mg/dL, 236.20 mg/dL, 175.40 mg/dL, and 117.20 mg/dL, respectively. The hypothesis test results showed a significant difference in 120 minutes OGTT blood glucose level (p= 0.031). Conclusion. The use of ethanol extract from S. crispus leaves is more beneficial in reducing blood glucose levels in 120 minutes OGTT compared to FBG level and 60 minutes OGTT level, with the highest reduction observed in the group given a dose of 600 mg/kgBW/oral/day compared to control. Keywords: DM, fasting blood glucose, OGTT, Rattus norvegicus, Strobilanthes crispus
THE EFFECT OF DRY RED JUJUBE FRUIT EXTRACT ON RATE ALKALINE PHOSPHATASE INDUCED WISTAR RATS ACETAMINOPHEN Maria Aloysia Praldinya Ere; Adi Pramono Hendrata; Laura Wihanto
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 5, No 3 (2023): July
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwmj.v5i3.4828

Abstract

Background: Many things can cause hepatotoxicity, including the use of chemicals such as acetaminophen consumed in toxic doses. Acetaminophen destroyed can damage liver tissue, block the biliary tract and end the release of ALP into the blood. Jujube fruit has antioxidants like flavonoids and phenolic, which can ward off free radicals due to excessive consumption of acetaminophen. Purpose: Knowing how giving dried red jujube fruit extract affects ALP levels of induced Wistar rats acetaminophen. Method: The method used is Posttest-Only Control Group Design. The technique used in examining ALP levels is using a colorimetric test essay which refers to the International Federation of Clinical Chemistry and Laboratory Medicine. The experimental animals used were Wistar rats which were given dried red jujube fruit extract doses of 70, 140, and 280 mg/kg BW for ten days. On the 9th day, the rats have induced with an acetaminophen dose of 3 g/kg BW, and on the 11th day, termination was carried out to examine ALP levels in the blood. Results: In this study, the normality test was generally distributed with a value of K(-) = 0.086, K(+) = 0.600, Kp1 = 0.156, Kp2 = 0.074, and Kp3 = 0.446. In the homogeneity test, the value of p = 0.0254 was obtained. On testone-way ANOVA, significant results were obtained with a p = 0.00. In the correlation test, a matter of 0.00 was obtained, which means a relationship exists between dried red jujube fruit extract and ALP levels. Conclusion: Giving dried red jujube fruit extract has an effect on ALP levels in induced rats' acetaminophen toxic dose.
Analisis Jalur: Gangguan Fungsi Nefron Tikus (Rattus Norvegicus) Akibat Mengkonsumsi Partikel Mikroplastik Polietilena: Pathway Analysis: Disorders of Nephron Function in Rats (Rattus Norvegicus) Due to Intake of Polyethylene Microplastic Particles Yudhiakuari Sincihu; Mujib Hannan; Niluh Suwasanti; Laura Wihanto; Nita Kurniawati; Dewa Ayu Liona Dewi; Steven; Sianty Dewi; Ari Christy Mulyono; Irene Lingkan Parengkuan; Henry Ricardo Handoyo; FX Himawan Jong; Andre Young; Alvin Julian; Angeline Rivia Simanjuntak; Marion Florentia
Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Vol. 7 No. 1: JANUARY 2024 - Media Publikasi Promosi Kesehatan Indonesia (MPPKI)
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/mppki.v7i1.4240

Abstract

Latar belakang: Pencemaran plastik menjadi isu kesehatan saat ini. Partikel plastik berukuran mikron (MPs) dapat mengganggu kesehatan karena terkonsumsi secara tidak sengaja oleh mahluk hidup dan masuk kedalam rantai makanan manusia. Polietilena salah satu polimer plastik yang banyak mengkontaminasi makanan manusia. Partikel plastik yang terabsorbsi akan mengalami persisten dan bioakumulasi dalam darah. Partikel ini mengandung bahan toksik sehingga menimbulkan reaksi oksidasi pada komponen biologi, salah satunya low-density lipoprotein (LDL). Ox-LDL menyebabkan kerusakan pertahanan nefron sehingga memicu kaskade kerusakan. Indikator kerusakan fungsi nefron ginjal berupa serum albumin, blood urea nitrogen, dan serum kreatinin. Tujuan: Menjelaskan mekanisme gangguan fungsi nefron akibat mengkonsumsi partikel MPs polietilena. Metode: Penelitian eksperimental murni pada 42 ekor tikus (Rattus norvegicus) yang dikelompok secara random menjadi 6 kelompok. Desain penelitian adalah post-test only control group. Dosis paparan yakni 0mg; 0,0375mg; 0,075mg; 0,15mg, 0,3mg, 0,6mg partikel MPs per hari diberikan secara berurutan pada setiap kelompok. Pemberian bahan MPs menggunakan sonde oral selama 90 hari. Data di analisis menggunakan program Smart-PLS untuk membuat model mekanisme gangguan fungsi nefron akibat mengkonsumsi partikel MPs. Hasil: Paparan peroral partikel MPs pada tikus secara signifikan meningkatkan kadar partikel MPs dalam darah dan Ox-LDL darah. Keberadaan MPs dalam darah juga akan meningkatkan Ox-LDL darah, selain itu menurunkan kadar serum albumin, meningkatkan kadar blood urea nitrogen dan serum kreatinin darah. Sedangkan peningkatan Ox-LDL hanya berkontribusi terhadap terjadinya penurunan kadar serum albumin dan peningkatan kadar blood urea nitrogen darah (semua nilai T-stat > 1,96). Kesimpulan: Mengkonsumsi partikel MPs dapat menyebabkan gangguan fungsi nefron pada tikus.
Moringa oleifera Leaf Ethanol Extract Inhibits Toxoplasma gondii Tachyzoites Replication Wihanto, Laura; Waworuntu, Gladdy Lysias; Tedyanto, Cecilia Putri; Puspitasari, Heni
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 1 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i1.42672

Abstract

The various infection routes of Toxoplasma gondii that are close to daily life strongly support the incidence of toxoplasmosis. The emergence of drug-resistant Toxoplasma gondii strains raises future concerns. Moringa leaf ethanol extract has been shown to have several anti-pathogen activities, which could have an anti-Toxoplasma effect. This research was conducted to analyze the anti-Toxoplasma effect of moringa leaf ethanol extract against tachyzoites replication in Toxoplasma gondii and the correlation between extract doses with the number of tachyzoites. Mice were divided into five groups. The negative control group (Group I) received CMC-Na solution. The positive control group (Group II) received spiramycin 100 mg/kg BW. The treatment groups received moringa leaf ethanol extract 250 mg/kg BW (group III), 500 mg/kg BW (group IV), and 1000 mg/kg BW (group V), respectively. Mice were injected with 1 x 105 tachyzoites/0.1 mL/mice intraperitoneally on the first day. Moringa leaf ethanol extract and spiramycin were given orally once daily for three days. The number of tachyzoites in the intraperitoneal fluid was calculated on the fifth day. The results have shown that there were significantly lower differences (P < 0.05) in group IV (P = 0.021) and group V (P = 0.022) compared to group I. There was also a significant negative correlation between the extract doses and the number of tachyzoites (P = 0.000; r = -0.781). Moringa oleifera leaf ethanol extract has an anti-Toxoplasma effect by inhibiting the tachyzoite replication at     500 mg/kg BW and 1000 mg/kg BW.
Layanan Telemedicine Terintegratif Dokter dan Apoteker bagi Penderita Covid-19 di Lingkungan Paroki Kristus Raja Surabaya Caroline Caroline; Senny Yesery Esar; Martha Ervina; Wuryanto Hadinugroho; Steven Steven; Yudhikuari Sincihu; Laura Wihanto
Jurnal Abdimas Kesehatan (JAK) Vol 4, No 3 (2022): November
Publisher : Sekolah Tinggi Ilmu Kesehatan Baiturrahim Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36565/jak.v4i3.388

Abstract

The global pandemic related to the coronavirus (2019-nCoV) continues to hit the world, including Indonesia until the end of 2021. The efforts to improve health services were carried out by various parties, including by members of the King of Christ Parish church as part of the Surabaya community. From this parish in July 2021, 301 people were sick with COVID-19 and almost 11% of them died. The public and health services were panicked by the occurrence of a wave of viral mutations, with the increasing of sufferers and high mortality rates. Integrated telemedicine services between doctors and pharmacists are one of the best options to prevent transmission and severity to support patient recovery rates. With telemedicine, health care needs are individualized and patient development is more controlled. The method was carried out through direct consultation between doctors, pharmacist, and patients, using the whatsapp call platform. Monitoring of patients was continued by pharmacist using the whatsapp message platform until the patient was cured. If necessary, consultation with the doctor could be carried out again during the patient’s healing process.  The research show that there is an integration of doctor and pharmacist services in telemedicine for COVID-19 patients in diagnosing, determining the best supporting drugs and therapies for the patients. Quantitatively, there was a decrease in the mortality rate, an increase in patient compliance and recovery. As an additional indicator, the residents welcome the integrated services that are carried out and can be developed for other health services.
Analisis Hambatan Perkembangan Stadium Plasmodium Falciparum Terhadap Pemberian Ekstrak Daun Salam Badak (Acmena Acuminatissima) In Vitro Laura Wihanto; Hilkatul Ilmi; Aty Widyawaruyanti; Achmad Fuad Hafid; Indah Tantular
JURNAL WIDYA MEDIKA Vol. 5 No. 2 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v5i2.2203

Abstract

Background: The urgency generated by drug-resistant strains of malaria parasites has accelerated the need to find a new anti-malarial drug. Recent studies have shown that 80% ethanol extract Acmena acuminatissima has suitable antimalarial activities. Aim: To analyze the inhibitory effect of A. acuminatissima extract at the developmental stage of P. falciparum in infected erythrocytes in vitro. Methods: the synchronized culture of P. falciparum was incubated along with A. acuminatissima extract, and compared with negative controls. The effect on parasite growth was determined by observing the level of parasitemia and accumulation of various infective stages seen on thin blood smears after the incubation period of 12, 24, 36, and 48 hours using a light microscope with 1000 times magnification. Results: Within 48 hours of observation the difference was mainly in the proportion of ring-stage which was 2.75% in culture with the test compound and 41.41% in the negative control which showed that ethanol extract of A. acuminatissima inhibited the growth of parasites at the schizont stage into the ring stage. In trophozoite and schizonts stages of culture with test compounds, enlargement of digestive vacuole and vesiculation is obtained. Conclusion: A. acuminatissima inhibits the development of intraerythrocyt parasites at the schizonts stage and induced morphological changes.
EFFECTS OF ETHANOL-BASED ANTISEPTIC SOLUTIONS AGAINST GRAM NEGATIVE BACTERIA Laura Wihanto; Handi Suyono
JURNAL WIDYA MEDIKA Vol. 7 No. 1 (2021): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v7i1.2833

Abstract

Introduction : Gram-negative bacteria infections cause diseases namely skin infection until sepsis including nosocomial infection. Prevention by antiseptic application is the way to inhibit infection. Some antiseptic compounds that have been used show resistance according to some reports. Aim: Determine the efficacy of ethanol-based antiseptic solutions against Gram-negative bacteria Methods : Discs saturated with ethanol-based antiseptic solutions were affixed to Muller Hinton agar which had been smeared by Acinetobacter baumannii ATCC BAA-747, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumonia ATCC BAA-1706, Escherichia coli ATCC 25922, Salmonella sp. , and Proteus vulgaris ATCC 6380. The diameter of the inhibition zone was read after 24 hours. Solutions are considered sensitive if the zone of inhibition of growth diameter is more than 6 millimeters (Kirby-Bauer disc diffusion method). Result : Acinetobacter baumannii and Salmonella sp is sensitive to solution consisting of ethanol 80 % and ethanol 80 %+ H2O2 0,15% . Pseudomonas aeruginosa and Eschericia coli is sensitive only to solution consisting of ethanol 80 %+ H2O2 0,15% As for Klebsiella pneumonia and Proteus vulgaris, they are resistance to both solutions. Conclusion: The use of ethanol-based antiseptic solutions with or without H2O2 0.15% addition may not effectively eliminate all gram-negative bacteria from the environment. The addition of 0.15% H2O2 to the antiseptic solution showed a better barrier effect than the solution containing only 80% ethanol. Adding other additives needs to be investigated further to formulate a better antiseptic solution against Gram-negative bacteria.
Co-Authors Achmad Fuad Hafid Alvin Julian Andre Young Angeline Rivia Simanjuntak Ari Christy Muliono Ari Christy Mulyono Aty Widyawaruyanti Cundawan, Robertus S de Fatima, Julio CA Dewa Ayu Liona Dewi FX Himawan Jong Gunawan, Alvin Halim, Stefan W Handi Suyono Handoyo, Henry Ricardo Hendrata, Adi Pramono Heni Puspitasari Hilkatul Ilmi Indah Tantular Irene Lingkan Parengkuan Maria Aloysia Praldinya Ere Marion Florentia Martha Ervina Mujib Hannan Niluh Suwasanti Nita Kurniawati Senny Yesery Esar Sentot Santoso Sianty Dewi Silvia Sutandhio Steven Steven Steven Steven Wijono Steven, . Tedyanto, Cecilia Putri Waworuntu, Gladdy L. Wuryanto Hadinugroho Yudhiakuari Sincihu Yudhikuari Sincihu