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Perbandingan Aktivitas Antibakteri 4-Metilpiperazin-N-Karbonil Ampisilin dengan Ampisilin Terhadap Micrococcus luteus dan Escherichia coli Pudjianto, Andreyanus Eko; Ervina, Martha; Caroline, Catherine; Soekardjo, Bambang; Foe, Kuncoro
Jurnal Farmasi Sains dan Terapan Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Sains dan Terapan

Piperacillin has antimicrobial action but it is not absorbed from gastrointestinal tract Therefore, it needs structure modification to increase its absorption while keeping its antimicrobial action. The 4-methylpiperazin-N-carbonyl ampicillin was the product of reaction between ampicillin and 4-methylpiperazin-N-carbonyl chloride using Schotten Baumann method. The aim of this research was to compare the antibacterial activity between 4-methylpiperazin-N-carbonyl ampicillin and ampicillin. The comparison test of antibacterial activity was done with Kirby-Bauer disk diffusion susceptibility method on Mueller-Hinton agar which had been inoculated with Micrococcus luteus and Escherichia coli. Based on orientation studies, concentration of 4-methylpiperazin-N-carbonyl ampicillin and ampiCillin were 0.5 ppm against Micrococcus luteus and 500 ppm against Escherichia collin order to get visible zone of inhibition. The results showed that 4-methylpiperazin-Ncarbonyl ampicillin had lower antibacterial activity than ampicillin which was showed by its zone of inhibition which was lower compared to ampicillin in inoculated agar media of Micrococcus luteus and Escherichia coli.

Pelatihan Pembuatan Produk Sambal Kemasan Siap Wirausaha bagi Tenaga Kerja Cleaning Service Ignatius Radix Astadi Pranoto Jati; Farida Lanawati Darsono; Lisa Soegianto; Kuncoro Foe
ABDINE: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2022): ABDINE : Jurnal Pengabdian Masyarakat
Publisher : Sekolah Tinggi Teknologi Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52072/abdine.v2i2.374

Tujuan pelatihan ini adalah memberikan bekal kepada para peserta mulai dari pemahaman terkait kualitas dan pemilihan jenis bahan baku serta proses pembersihan dan pengemasan untuk produk sambal kemasan Pelatihan diharapkan mampu membuat para peserta mampu membuat secara mandiri produk sambal kemasan dan siap untuk dipasarkan sebagai calon wirausaha. Pelatihan proses pembuatan dan pengemasan untuk produk sambal kemasan dilaksanakan pada hari sabtu tanggal 25 Mei 2022. Metode pelaksanaan dengan memberikan penyuluhan di awal dan dilanjutkan dengan praktek secara langsung. Hasil pengabdian ini menunjukkan bahwa pemahaman dan pengembangan wawasan peserta terkait dengan proses pembuatan sambal dalam kemasan meningkat, sebagaimana ditunjukkan dengan peningkatan nilai indikator pre dan post test meskipun belum memenuhi angka indikator target, yaitu seluruh peserta berhasil membuat sampai mengemas produk sambal yang telah dibuatnya

2-(3-(chloromethyl)benzoyloxy)benzoic Acid Increases CD4+ Regulatory T-Cell Population and FoxP3 Expression in Lipopolysaccharide-induced Mice Yudy Tjahjono; Caroline Caroline; Jusak Nugraha; Kuncoro Foe; Srikanth Karnati; Süleyman Ergün; Nico Jafet; Oryza Chrisantia; I Made Andika Bara Kusuma; Hendy Wijaya; Wuryanto Hadinugroho; Dwi Aris Agung Nugrahaningsih; Dwi Liliek Kusindarta; Yufita Ratnasari Wilianto; Senny Yesery Esar; Hevi Wihadmadyatami
The Indonesian Biomedical Journal Vol 15, No 4 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i4.2429

BACKGROUND: Lipopolysaccharide (LPS) has been reported to increase CD4+ regulatory T-cell (CD4+ Treg) populations. Acetylsalicylic acid (ASA) has been reported to have immunomodulatory activity, but it may induce chronic gastric ulceration. Another salicylic acid-bearing compound, 2-(3-(chloromethyl)benzoyloxy)benzoic acid (3-CH2Cl), has been reported to have less gastric mucosal damage. However, the effect of 3-CH2Cl on CD4+ Tregs in LPS-induced mice is still unknown. Therefore, the present study was conducted to investigate the immunomodulatory effect of 3-CH2Cl on CD4+ T-cell and CD4+ Treg populations as well as FoxP3 expression in LPS-induced mice.METHODS: Synthesis of 3-CH2Cl was performed by mixing salicylic acid and chloromethylbenzoylchloride with the catalyzation of pyridine, acetone and heat. The 3-CH2Cl tablets were prepared using direct compression method. After intraperitoneal injection of 1 mg/kg BW LPS to mice, 60 mg/kg BW ASA or 60 mg/kg BW 3-CH2Cl was given orally for 3 days. The splenocyte was obtained through splenectomy and collagenase digestion. The population of CD4+ T-cells and CD4+ Tregs, as well as the splenic FoxP3 expression were determined using flow cytometry technique.RESULTS: CD4+ T-cell populations in mice treated with LPS and 3-CH2Cl or ASA were lower than those treated with LPS merely. Meanwhile, CD4+ Treg populations and FoxP3 expression levels in mice treated with LPS and 3-CH2Cl or ASA were higher than those treated with LPS merely.CONCLUSION: Since 3-CH2Cl could decrease CD4+ T-cell population and increase CD4+ Treg population mediated by the increase of FoxP3 expression in LPS-induced inflammation, it may act as a potential therapeutic drug to reduce inflammatory conditions.KEYWORDS: 2-(3-(chloromethyl)benzoyloxy)benzoic acid, acetylsalicylic acid, CD4, T-regulatory cells, FoxP3, LPS

Development of standardized green coffee bean extract (Coffea canephora) into effervescent granules as an antioxidant supplement Wijaya, Andre; Darsono, Farida Lanawati; Foe, Kuncoro
Pharmaciana Vol. 14 No. 3 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i3.29237

This study aimed to obtain the optimum formula of effervescent granules of green coffee extract (EG-GCE) on its physical quality (flow speed, water content, and dissolving granule time test) and effectiveness as an antioxidant. The dried extract was obtained by percolation of green coffee with water and dried using a spray drier. The dry extract will be standardized for specific and non-specific. The dose of the dried extract of green coffee used in the granule effervescent was 250 mg pr sachet. EG-GCE was formulated using wet granulation method. Effervescent granules were tested for physical quality (organoleptic, pH, moisture content, flow properties, and dissolving effervescent granule time test) and effectiveness, consisting of antioxidant activity (IC50) with the DPPH method using the microplate reader. The optimum effervescent granule formula was obtained using the factorial design method. The factors used were citric acid monohydrate with a level of (-1) 8% and a level of (+1) 12%, and tartaric acid with a level of (-1) 16%and a level of (+1) 24%. The determination of the optimum formula (proportion of citric acid monohydrate and tartaric acid) was carried out by factorial design method using the following responses: moisture content, flow rate, and dissolving effervescent granule time test. Furthermore, the One Way Anova (Yate's Treatment) statistical method will analyze data from parametric experiments between batches and between formulas. If there is a significant difference in the statistical analysis between the formulas, then the test is continued using the Tukey post-hoc test method. The pH value of resulting EG-GCE products was within the range of 5.46-6.07, moisture content: 3.12-3.67%, flow rate: 25.78-28.53 g/s, angle of repose: 25.65-30.130, Hausner ratio: 1.14-1.22, Carr's index: 12.50–17.83%, dissolving effervescent granule time test: 1.00-1.33 min. This study demonstrated that citric acid monohydrate, tartaric acid, and their interaction affected the moisture content, flow rate, and effervescent time of EG-GCE. The proportion of citric acid monohydrate (9.94%) and tartaric acid (17.46%) was found to be the optimum formula of EG-GCE, with the following responses: moisture content 3.26%, flow rate 25.72 g/s, and dissolving effervescent granule time test 1.19 min. The optimum formula show strong antioxidant activity with IC50 free of radical scavenging 56.56 ± 0.97 µg/ml.

Uji Aktivitas Analgesik Tablet Asam 4-(Klorometil)Salisilat pada Mencit Putih dengan Metode Writhing Test dan Hotplate Tiffany, Angela; Ribka Abiati, Meidelin; Foe, Kuncoro; Tjahjono, Yudy; Caroline; Esar, Senny Yesery; Hadinugroho, Wuryanto
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol. 14 No. 1 (2022): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Acetylsalicylic acid is an analgesic drug that still popularly used. However, acetylsalicylic acid may result undesirable side effect of gastrointestinal as it can cause gastric ulcer to bleeding. Previous research has succeeded synthesizing new compound derived from salicylic acid, namely 4-(chloromethyl)salicylic acid which has the potential to reduce its side effects with a better pharmacological effects than acetylsalicylic acid. In its development, the formulation of the compound in tablet dosage form has been carried out. However, until now there has not been a pharmacological test to determine whether it still provides a greater effect than acetylsalicylic acid after being formulated. This study is to test the analgesic activity of 4-(chloromethyl)salicylic acid tablets in albino mice using writhing test and hotplate methods. Analgesic activity test using the hotplate method was carried out on albino mice which given the suspension of the tablet sample and the observations were made in a periodic of time. Analgesic activity test using writhing test method was carried out in 0.6% acetic acid-induced albino mice, where the suspension of the tablet was administered 30 minutes before administration of acetic acid. Analgesic activity was assessed by counting the number of writhings, and the results were statistically compared with one-way ANOVA. Tests were also performed on acetylsalicylic acid as a positive control and suspension as a negative control group. There were significant differences (P<0.05) of the analgesic activity observed among groups. Both methods show that 4-(chloromethyl)salicylic acid tablets provide stronger analgesic activity than acetylsalicylic acid.

Perbandingan Aktivitas Antibakteri 4-Metilpiperazin-N-Karbonil Ampisilin dengan Ampisilin Terhadap Micrococcus luteus dan Escherichia coli Pudjianto, Andreyanus Eko; Ervina, Martha; Caroline, Catherine; Soekardjo, Bambang; Foe, Kuncoro
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 1 No. 1 (2013)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v1i1.2342

Piperacillin has antimicrobial action but it is not absorbed from gastrointestinal tract Therefore, it needs structure modification to increase its absorption while keeping its antimicrobial action. The 4-methylpiperazin-N-carbonyl ampicillin was the product of reaction between ampicillin and 4-methylpiperazin-N-carbonyl chloride using Schotten Baumann method. The aim of this research was to compare the antibacterial activity between 4-methylpiperazin-N-carbonyl ampicillin and ampicillin. The comparison test of antibacterial activity was done with Kirby-Bauer disk diffusion susceptibility method on Mueller-Hinton agar which had been inoculated with Micrococcus luteus and Escherichia coli. Based on orientation studies, concentration of 4-methylpiperazin-N-carbonyl ampicillin and ampiCillin were 0.5 ppm against Micrococcus luteus and 500 ppm against Escherichia collin order to get visible zone of inhibition. The results showed that 4-methylpiperazin-Ncarbonyl ampicillin had lower antibacterial activity than ampicillin which was showed by its zone of inhibition which was lower compared to ampicillin in inoculated agar media of Micrococcus luteus and Escherichia coli.

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