Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Agrotrop : Journal on Agriculture Science

Optimasi Isolasi Protoplas Mesofil Daun Anggrek Paraphalaenopsis laycockii EDY SETITI WIDA UTAMI; SUCIPTO HARIYANTO
Agrotrop : Journal on Agriculture Science Vol 5 No 1 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.546 KB)

Abstract

Optimalization protoplast isolation of the leaves mesophyll orchid Paraphalaenopsis layockii. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii. The factorial experiment consisted of two factors that was arranged by completely randomized design (CRD) with ten replications. The first factor was concentrations of sucrose which consists of three levels namely 0.4 M, 0.5 M and 0.6 M. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. The results showed that there was effect of treatment for all variables observed. The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.6 M sucrose and 4 hours of incubation time.
Pengaruh Pemberian Ekstrak Pisang pada Media VW terhadap Induksi Akar dan Pertumbuhan Tunas Dendrobium lasianthera J.J.Sm EDY SETITI WIDA UTAMI; SUCIPTO HARIYANTO; Y. SRI WULAN MANUHARA
Agrotrop : Journal on Agriculture Science Vol 6 No 1 (2016)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.135 KB)

Abstract

The Effect of Banana Extract at The VW Media on Root Induction and Growth of ShootDendrobium Lasianthera J.J.Sm. Orchid has economic potential as a non-oil exportcommodities which can increase foreign exchange. In addition to supporting industry as cutflowers and potted plants, Dendrobium orchid in particular has been widely recognized as atraditional medicine. Orchids can be propagated in vitro using seed. The main problem in thepropagation of orchid through seed culture is difficulty in root formation. In this study, weevaluated the effect of organic compounds of the banana extract at Vacin and Went mediumcontaining peptone 2 gL-1 to induce growth of root and shoot of the Dendrobium lasianthera.The shoot measuring about 0.3-1.0 cm contain of 1-2 leaves obtained from the seedgermination was cultured on VW medium containing 2 gL-1 peptone additives with 50 gL-1,100 gL-1 and 150 gL-1 banana extract, and without banana extract was used as control. After16 weeks of culture, number of roots, number of leaves, root length, leaf length, the shootheight, root and leaf widest diameters were recorded. Data were statistically analyzed usingANOVA and continued by honestly significance difference (HSD). The results showed thatthe extract of banana statistically gave a significant effect on root induction and growth ofshoot D. lasianthera.