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AKTIVITAS ANTIBAKTERI DAUN KENTUT DAN EKSTRAK BUAH BELIMBING TERHADAP ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS Melati Yulia Kusumastuti; Samran Samran; Debi Meilani
Collaborative Medical Journal Vol 5 No 1 (2022): Januari
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/cmj.v5i1.2100

Abstract

Bacteria is one of the factors that cause infection. These infections can be treat with antibacterial compounds. Plants are one of the greatest sources of antibacterial compounds. Leaves of skunk vine (Paederia foetida L.) and sweet star fruit (Avverhoa carambola L.) are plant parts that commonly used traditionally as wound healing, anti-inflammatory, anti-diarrhea, etc. The purposes of this study were to determine the antibacterial activity of the ethanol extract of skunk vine leaves and sweet starfruit leaves and to determine minimum inhibitory concentration (MIC) against E. coli and S. aureus. Leaves of skunk vine and sweet star fruit were extracted with 96% ethanol. The ethanol extract of each plant was screened for their phytochemicals compound and tested for antibacterial activity. The antibacterial activity test was carried out by using the agar disc diffusion method. The results of the study showed that 500 mg/ml of ethanolic extract of skunk vine leaves and sweet star fruit leaves could inhibited the growth of E. coli bacteria around 28.29 mm and 21.50 mm. Meanwhile, both extracts in the same concentration showed inhibition zones against S. aureus around 24.45 mm and 22.53 mm. It showed that ethanol extract of skunk vine leaves has better antibacterial activities than ethanol extract of start fruit leaves. The MIC of skunk vine leaves extract on both bacteria was 200 mg/ml, while the MIC of sweet starfruit leaves ethanol extract for E. coli was 12.5 mg/ml and S. aureus was 25 mg/ml. This indicates that both ethanol extracts have antibacterial activity against E. coli and S. aureus bacteria.
Aktivitas Antibakteri Ekstrak, Fraksi Kloroform dan Fraksi n-Heksan Daun Kemangi terhadap Staphylococcus aureus dan Eschericia coli Melati Yulia Kusumastuti; Debi Meilani; Suhendra Tawarnate
Jurnal Indah Sains dan Klinis Vol 2 No 1 (2021): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/jisk.v2i1.11

Abstract

Basil (Ocimum citriodorumVis.) is a plant which is their leaves are commonly eaten as fresh vegetables and have many benefits. Basil leaves contain secondary metabolite compounds that have antibacterial activity such as flavonoids, tannins, saponins, alkaloids, and steroids. The purpose of this study was to known the antibacterial activity of some fractions of basil leaves against Escherichia coli and Staphylococcus aureus and see which fraction was more active. This research included extraction by maceration method using ethanol 96%, then ethanol extract was fractionated using n-hexane and chloroform, phytochemical screening, then tested for antibacterial activity against E. coli and S. aureus by used agar diffusion method. Phytochemical screening showed ethanol extractcontained alkaloids, saponins, flavonoids, tannins, and steroids; chloroform fraction contained alkaloids, flavonoids, saponins, and tannins; and n-hexane fraction contained steroids. The results of the antibacterial activity test showed ethanol extract and chloroform fraction had antibacterial activity. Meanwhile, the n-hexane fraction did not have antibacterial activity. The concentration of 500 mg/ml of chloroform fraction and ethanol extract on S. aureus resulted from an inhibition zone around 24.67-26.90 mm. Furthermore, E. coli produced 22.67-23.84 mm. Ethanol extract had higher antibacterial activity than the chloroform fraction against E. coli and S. aureus
Evaluasi Stabilisator Agar-Agar dan CMC Sediaan Sirup Markisa Berastagi Suprianto; Muhammad Gunawan; Melati Yulia Kusumastuti; Cut Fatimah; Debi Meilani
Jurnal Indah Sains dan Klinis Vol 3 No 1 (2022): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/b1pzvs47

Abstract

Fruit syrup is made from a sugar solution, the taste and aroma are determined by the freshness of the fruit. Fruit commonly used in making syrup has an attractive color, aroma and distinctive taste. Passion fruit (Passiflora edulis Sims.) has a distinctive taste and aroma, so it is widely processed into syrup. This study was to evaluate CMC and Agar-Agar stabilizers in the passion fruit syrup formula. Evaluation includes color, appearance, texture, taste and aroma and hedonic tests are carried out. The results showed that there were significant differences in the color, appearance, texture, taste and aroma of passion fruit syrup. Stabilizers CMC and Agar-Agar make the color, texture and appearance of the syrup better and more attractive. The result of hedonic test for ideal syrup concentration is S3 with 4 g of Agar and 6 g of CMC. The higher the concentration of CMC and Agar, the greater the viscosity of the syrup. The concentration of CMC and Agar-agar in a mixture of fruit juices gave significantly different effects on color, texture, appearance, taste and aroma
Evaluasi Konsentrasi Karboksimetilselulosa Alginat Formula Pasta Gigi Antiseptik Ekstrak Etanol Daun Seledri (Apium graveolens L.) Samran; Suprianto; Muhammad Gunawan; Melati Yulia Kusumastuti
Jurnal Indah Sains dan Klinis Vol 3 No 1 (2022): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/jisk.v3i1.45

Abstract

Maceration of Celery leaves using 96% ethanol solvent was used to make the extract. Toothpaste is formulated with carboxymethylcellulose and alginate binders. The active ingredient in the form of ethanol extract from Celery leaves has the potential for antibacterial. This study was to evaluate the concentration of carboxymethylcellulose alginate in the preparation of antiseptic toothpaste of ethanol extract from Celery leaves. Evaluation of production results consisted of organoleptic, homogeneity, spreadability, foam formation, acidity and viscosity. The higher the concentration of carboxymethylcellulose, the lower the foam and spreadability and the higher the viscosity, while the alginate concentration is the opposite. Carboxymethylcellulose alginate affects the evaluation parameters of antiseptic toothpaste preparations ethanol extract of Celery leaves
Analisis Biaya Efektif Terapi Diare Pasien Rawat Inap RS Putri Hijau Medan Muhammad Gunawan; Suprianto; Melati Yulia Kusumastuti; Sumardi; Debi Meilani
Jurnal Indah Sains dan Klinis Vol 3 No 1 (2022): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/jisk.v3i1.46

Abstract

Diarrhea is characterized by the excretion of liquid stools with a frequency of three or more times a day, due to gastrointestinal infections due to food and water contaminated with microorganisms, or directly from humans due to poor sanitation. This study was to determine the characteristics of diarrhea patients, treatment and cost-effectiveness of treatment at Putri Hijau Hospital, Medan. The non-experimental research is descriptive and retrospective in nature of financial patient medical records from July to December 2018. Cost Effectiveness Analysis as a method of cost analysis. The characteristics of the patients obtained were 14 women (46.670%) and 16 men (53.33%). The effectiveness of New Diatabs (92.30%) and New Diatabs Metronidazole (94.11%). The cost of therapy with New Diatabs is Rp. 1,547,771/patient and New Diatabs Metronidazole is Rp. 1,562,590/patient. Average cost-effectiveness New Diatabs Rp. 190,345 and New Diatabs Metronidazole Rp. 183,060, so it is concluded that the cost-effective therapy is the use of the combination of New Diatabs Metronidazole compared to New Diatabs.
Total Plate Numbers (ALT) Check Andyeast Mold Numbers (YM) In Kunyit Asam Jamu Melati Yulia Kusumastuti; Safriana Safriana; Siti Aisyah Tanjung; Ainul Mardhiah
International Journal of Health and Social Behavior Vol. 2 No. 1 (2025): February: International Journal of Health and Social Behavior
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/ijhsb.v2i1.257

Abstract

Turmeric and tamarind herbal medicineis a medicinal herb in liquid form that is made from turmeric (Curcuma domestica Val.), and tamarind fruit (Tamarindus indica L.) produced by the home industry. Jamu Gendong does not require a license to distribute, but the quality of herbal medicine must be so that it is safe for consumption. The purpose of this study was to determine the presence or absence of microbial contamination in the form of bacteria and molds/hamirs in the preparation of Jamu Gendong Kunyit Asam circulated in Harjosari I Village, Medan Amplas Subdistrict. The samples were taken from four locations: Jalan Garu II, Garu III, Garu IV, and Garu VI. The total plate count (PCA) test was carried out by dilution of stratified samples using Lactose Broth (LB) media, for bacterial tests 10 -1 to 10-5 dilutions, and for fungal tests 10-1 to 10-3 dilutions. The diluted samples were placed in medium and incubated at 45o±1o in an incubator. The bacterial total plate count was performed using Plate Count Agar (PCA) as the medium. It was incubated at 35-37oC for 24 h, and the mold/fungal test used Potato Dekstro Agar (PDA) as media and incubated at 20-25oC for 48 h. For comparison, we used packaging for herbal medicines purchased from supermarkets. Furthermore, the number of bacteria and fungi in the colon was observed and counted. The results showed fungal contamination in the home industry at Jamu Kunyit Asam. It was a sample collected from Garu II, a sample of 86×103. The samples had many colonists that exceeded the regularity set up in BPOM RI No. 32 of 2019 and SNI 19-2879-1992.
Penyuluhan Pengenalan Makanan Bergizi Dan Bernutrisi Melati Yulia Kusumastuti; Cut Fatimah; Muhammad Gunawan; Pebrinawanti Br Saragih; Dyan Imelda; Sellyana Sitio; Sri Wahyuningsih
Sevaka : Hasil Kegiatan Layanan Masyarakat Vol. 3 No. 2 (2025): Mei : Sevaka : Hasil Kegiatan Layanan Masyarakat 
Publisher : STIKES Columbia Asia Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62027/sevaka.v3i2.352

Abstract

Bingkat Village is one of the villages located in Pegajahan District, Serdang Bedagai Regency, North Sumatra Province, with an area of 5.95 km2 or 595.96 hectares divided into 10 hamlets. Most of the land area in Bingkat Village is used as rice fields, which is 60.740% of the total land area and is used as fields and plantations of 26.830% of the total land area, so that the majority of heads of families in Bingkat Village are farmers. Healthy food is food that contains balanced nutrition, contains fiber and substances needed by the body for the child's growth and development process. Balanced nutrition can be useful as an energy substance, a regulator, and a building substance. Food also greatly affects children's digestion, so parents must be able to provide healthy food that can maintain children's digestion. So that it does not interfere with children's growth. Healthy food for digestion should be rich in nutritional elements such as carbohydrates, proteins, minerals, vitamins, and a little unsaturated fat, or more precisely abbreviated as the 4 healthy 5 perfect menu. Through this program by conveying about consuming Good and Healthy Nutritional and Nutritious Food. So that the community will avoid mistakes in choosing food and the negative impacts that may arise. The type of research used is quantitative descriptive research on survey methods through interviews.. Based on the results of research that has been conducted in Bingkat Village with a total of 19 respondents, 72% know about choosing nutritious and nutritious food and 28% know about food additives (BTM), then the level of community knowledge about nutritious and nutritious food is higher compared to the level of community knowledge about food additives (BTM).
Pembuatan Sabun Cair Cuci Tangan Antiseptik Menggunakan Bahan Alami Tumbuhan Serai Safriana; Melati Yulia Kusumastuti; Enny Fitriani; Abro; Asmaida Nasution; Muhammad Razan; Olivia Anggrella; Windi Utami
Sevaka : Hasil Kegiatan Layanan Masyarakat Vol. 3 No. 2 (2025): Mei : Sevaka : Hasil Kegiatan Layanan Masyarakat 
Publisher : STIKES Columbia Asia Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62027/sevaka.v3i2.366

Abstract

Antiseptic hand wash liquid soap is a soap that contains certain chemicals to reduce and prevent bacterial infections. Hand washing liquid soap that uses natural ingredients from lemongrass plants (Cymbopogon nardus) has a variety of significant benefits and functions. Lemongrass (Cymbopogon nardus) contains essential oils with antibacterial and antimicrobial properties, making it effective in killing germs and bacterial on hands. This is very important in preventing the spread of disease, especially in vulnerable environments. This hand wash liquid soap is also safe for the skin, reducing the risk of irritation that often occurs due to the use of chemical hand wash liquid soap, and is suitable for all skin types including sensitive skin. The use of lemongrass (Cymbopogon nardus) based hand washing liquid soap for the community is very broad. In addition to serving as an educational tool in public health campaigns, this product also supports the local economy by utilizing raw materials from local farmers, thus promoting sustainable agriculture and creating jobs. With the increasing awareness of the importance of hand hygiene, this liquid hand soap is a healthy alternative for consumers who care about health and the environment. Overall, the use of lemongrass (Cymbopogon nardus) based hand wash liquid soap not only contributes to individual health, but also improves the quality of life of the community through disease prevention and support to the local economy.