Article Per Year (5 Year)
p-Index From 2021 - 2026
1.116
P-Index
This Author published in this journals
All Journal The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Mutiara Medika: Jurnal Kedokteran dan Kesehatan Dental Journal (Majalah Kedokteran Gigi) Jurnal Perikanan Universitas Gadjah Mada Media Medika Muda Odonto dental journal
Anne Handrini Dewi
Faculty Of Dentistry Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology

Published : 17 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 17 Documents
Search

 1   2 
Effect of Citrus aurantifolia swingle essential oils on methyl mercaptan production of Porphyromonas gingivalis Anindya Prima Yusinta; Ivan Arie Wahyudi; Anne Handrini Dewi
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 1 (2013): March 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.288 KB) | DOI: 10.20473/j.djmkg.v46.i1.p50-54

Abstract

Background: Halitosis is a term used to describe an unpleasant odors emanating timely from oral cavity. The unpleasant smell of breath most common caused from volatile sulphure compound (VSC). Methyl mercaptan is the major component of VSC. P. gingivalis produced large amount of methyl mercaptan. The essential oils of Citrus aurantifolia swingle contain antibacterial component. Purpose: The purpose of this study was to determine the effect of essential oil of Citrus aurantifolia swingle on the production of methyl mercaptan compounds in P. gingivalis. Methods: Bacterial suspension of P. gingivalis in TSB medium with 108 CFU/ml concentration cultured in a microplate and added by the essential oils of Citrus aurantifolia swingle with 1%, 2%, 3% and 4% concentration. Distilled water was used as negative control and 0.2% Chlorhexidine mouthwash was used as a positive control. Microplate was incubated anaerobically for 48 hours. After the periode of incubation, 0.6% methionine as the exogenous substrate and 0.06% DTNB as a reagen for determining methyl mercaptan concentration were added to each wells. The microplate was futher incubated for 12 hours. Concentration of methyl mercaptan produced by the P. gingivalis was measured spectrophotometrically using microplate reader at 415 nm. Results: One-way ANOVA showed that the essential oil of Citrus aurantifolia swingle take effect on the concentration of methyl mercaptan produced by P. gingivalis. LSD test results indicated that there was a significant difference of methyl mercaptan concentration between treatment groups of the essential oils of Citrus aurantifolia swingle and distilled water that used as negative control. Conclusion: The essential oil of Citrus aurantifolia swingle has decreased the production of methyl mercaptan produced by P. gingivalis.Latar belakang: Halitosis adalah istilah yang digunakan untuk menggambarkan bau tidak sedap yang berasal dari rongga mulut. Penyebab utama halitosis adalah senyawa volatile sulphur compound (VSC) dan metil merkaptan merupakan komponen VSC yang paling dominan menyebabkan halitosis. P. gingivalis dapat memproduksi metil merkaptan dalam jumlah banyak. Minyak atsiri kulit jeruk nipis (Citrus aurantifolia swingle) memiliki kandungan antibakteri di dalamnya. Tujuan: Untuk mengetahui pengaruh minyak atsiri kulit jeruk nipis terhadap produksi senyawa metil merkaptan pada bakteri P. gingivalis. Metode: Suspensi bakteri P. gingivalis dalam media TSB dengan konsentrasi 108 CFU/ml dibiakkan dalam microplate. Selanjutnya dilakukan penambahan minyak atsiri kulit jeruk nipis konsentrasi 1%, 2%, 3%, dan 4%. Obat kumur chlorhexidine 0,2% digunakan sebagai kontrol positif dan akuades sebagai kontrol negatif. Microplate diinkubasi selama 48 jam untuk selanjutnya dilakukan penambahan metionin 0,6% dan DTNB 0,06% dan diinkubasi kembali selama 12 jam. Konsentrasi senyawa metil merkaptan yang diproduksi oleh bakteri P. gingivalis dihitung dengan menggunakan microplate reader pada panjang gelombang 415 nm. Hasil: ANOVA satu jalur menunjukkan bahwa minyak atsiri kulit jeruk nipis berpengaruh terhadap konsentrasi metil merkaptan yang diproduksi oleh bakteri P. gingivalis. Hasil uji LSD menunjukkan adanya perbedaan yang signifikan antara kelompok perlakuan minyak atsiri kulit jeruk nipis dengan akuades sebagai kontrol negatif. Kesimpulan: Minyak atsiri kulit jeruk nipis dapat menurunkan produksi senyawa metil merkaptan yang dihasilkan oleh bakteri penyebab halitosis P. gingivalis.
PENGARUH SUBSTITUSI TULANG DENGAN HIDROKSIAPATIT (HAp) TERHADAP PROSES REMODELING TULANG Indah Lestari Vidyahayati; Anne Handrini Dewi; Ika Dewi Ana
Media Medika Muda Vol 1, No 3 (2016)
Publisher : Faculty of Medicine Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar belakang: Kerusakan jaringan oleh patah jaringan, trauma, atau penyakit memerlukan restorasi untuk memperbaiki fungsinya seperti sedia kala. Beberapa penelitian telah dilakukan untuk menemukan bahan-bahan substitusi jaringan, termasuk mengembangkan bahan hidroxyapatite (HAp). HAp merupakan bahan bioaktif yang memiliki kemampuan osteokonduktif, bioaktivitas, dan biokompatibilitas dalam proses pembentukan tulang. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh substitusi tulang dengan hidroksiapatit (HAp) pada gambaran histologis tulang pada tibia tikus (Rattus Sprague Dawley).Penelitian dilakukan dengan 15 tikus jantan Sprague Dawley, usia 3 bulan, dengan membuat defek di tibia kanan dan tibia kiri. Setiap defek berukuran 3 mm x 1,5 mm x 1 mm. Sebagai perlakuan diberikan implantasi hidroksiapatit pada defek tibia kanan dan tibia kiri sebagai situs kontrol tanpa implantasi. Masing-masing subjek didekapitasi setelah 1, 2, 3, 4, dan 8 minggu. Daerah defek diambil dan dibuat gambaran histologi, kemudian dilakukan pengamatan dan penghitungan jumlah sel osteoblas, dan osteoklas menggunakan mikroskop kontras fase.Data dianalisis oleh SPS 2000 dengan metode Two Way Anava. Hasil penelitian menunjukkan bahwa substitusi tulang dengan hidroksiapatit tidak signifikan pada aktivitas osteoblas atau osteoklas ditunjukkan dengan p>0,05, tapi analisis antar waktu menunjukkan signifikasi pada osteoklas (p<0,05). Investigasi pada gambaran histologi menunjukkan peningkatan pada aktivitas pembentukan tulang baru pada defek tulang. Kata kunci: substitusi tulang; hydroxyapatite; sel-sel tulang; gambar histologi
Kemampuan Pembasahan Saliva Buatan dengan Kandungan Ekstrak Mukus Lele (Clarias batrachus) Zipora Silka Yoretina; Anne Handrini Dewi; Hendri Susanto; Heribertus Dedy Kusuma Yulianto
Jurnal Perikanan Universitas Gadjah Mada Vol 23, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.62571

Abstract

Xerostomia merupakan keluhan subyektif mulut kering yang sering berhubungan dengan adanya penurunan fungsi kelenjar saliva. Penanganan pasien xerostomia dapat dilakukan dengan pemberian saliva buatan. Mukus lele memiliki karakteristik dan kemampuan dalam menggantikan fungsi saliva, sehingga dengan konsentrasi yang tepat mampu berperan sebagai lubrikan yang baik. Penelitian ini bertujuan untuk mengetahui ada tidaknya kemampuan pembasahan antara saliva manusia dengan saliva buatan yang mengandung mukus lele (Clarias batrachus) pada permukaan hidrofobik. Mukus lele (Clarias batrachus) diekstraksi kemudian diformulasikan menjadi saliva buatan dengan menambahkan 0,1% methylparaben, 0,05% EDTA, 4,69% dekstrosa, dan aqua pro-injection. Pengujian dilakukan dengan mengukur kemampuan pembasahan saliva buatan menggunakan indikator besarnya sudut kontak sampel pada permukaan hidrofobik selama 5 menit. Data yang digunakan merupakan rerata nilai sudut kontak pada kedua sisi tetesan di menit ke-0, 0,5, 1, 1,5, 2, dan 5 yang dianalisis menggunakan aplikasi ImageJ. Hasil independent sample t-test  menunjukkan sudut kontak saliva buatan mukus lele lebih kecil daripada saliva manusia (p<0,05) pada pengamatan menit ke-0, 0,5, 1, 1,5, 2, dan 5. Kemampuan pembasahan saliva buatan mukus lele memiliki perbedaan yang signifikan dibandingkan dengan saliva manusia, sehingga saliva buatan ini memberikan fungsi lubrikatif dan protektif yang tidak terlalu menyerupai saliva manusia.
In-vitro cytotoxicity activity of potato (Solanum tuberosum. L) peel extracts against human gingival fibroblasts Khong Mei Xuan; Anne Handrini Dewi; Ivan Arie Wahyudi
Majalah Kedokteran Gigi Indonesia Vol 7, No 2 (2021): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.40196

Abstract

Potato peel is often regarded as waste although it contains phenolic compounds, glycoalkaloids, and flavonoid. This study aimed to evaluate the effect of different concentrations of potato peel extracts on the viability of Human Gingival Fibroblasts (HGF). Potato peel extracts were prepared by a maceration technique. The 96-well tissue culture micro titre plates were seeded with HGF at a density of 2×104 cells/100 μL and incubated for 24 hours. Next, 100 μL of potato peel extracts at a concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL and a medium (control) were dispensed into the well of the cell culture. Each concentration was evaluated for its viability with 3 replicate samples. The results of the MTT test were analyzed statistically using one-way ANOVA and LSD test. The mean and standard deviation of the viable HGF after incubated with the potato peel extract at the concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL were 98.67% ± 3.56, 88.34% ± 0.79, 55.42% ± 3.96, 28.33% ± 0.60, and 26.26% ± 0.53, respectively. The percentage of non-viable HGF increased with an increase in the concentration of the potato peel extract. The ANOVA test result showed a significant influence of various concentrations of the potato peel extract on the viability of HGF (p<0.05). The result of the LSD-test showed a significant difference among all the treatment groups (p<0.05). A higher concentration of potato peel extracts increased the viability of HGF cell line and the concentrations of 62.5 μg/mL and 125 μg/mL were considered non-cytotoxic.
EFFECTIVITY OF MULTIFUNCTION HERBAL TOOTHPASTE CONTAINING BAY LEAF (EUGENIA POLYANTHA WIGHT) EXTRACT AS EXTRINSIC STAIN REMOVAL ON TEETH AND DENTURE Mutiara Annisa; Archadian Nuryanti; Anne Handrini Dewi
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.079 KB) | DOI: 10.30659/odj.9.0.40-50

Abstract

Background: White teeth colour influence aesthetical appearance which leads to self-confidence and quality of life. Extrinsic stain is the most common aetiology of teeth discoloration, which caused by widely consumed products such as tea, coffee, and tobacco. It also formed on denture and cause negative impacts. Commercial extrinsic stain removal toothpastes only indicated for teeth and contain chemical active agent with undesirable effects. Aim of this study is to determine effectivity of bay leaf (Eugenia polyantha Wight) extract as toothpaste active agent for extrinsic stain removal on teeth and dentures.Method: Pictures of teeth, artificial teeth, and acrylic resin base taken before experiment, after stain induction (using 25g of tea in 100ml of 100oC water for 7 days, tea solution replaced every 24 hours), and after brushing. Toothpastes used for brushing were no active agent basic formulation toothpaste, bay leaf extract toothpastes at 5%, 10%, 15% concentrations, and commercial stain removal toothpaste as positive control. Specimens were brushed using automatic brushing machine for 70 seconds/surface, using 250g load, and speed 5 movements/sec. Colour index analysis of specimens’ pictures were computerized and analysed using CIELAB method to obtain L-value of extrinsic stain removal. Data analysis performed using one-way ANOVA (α=0.05).Result: There is effect of toothpaste containing bay leaf extract 15% to remove extrinsic stain both on teeth, artificial teeth, and acrylic resin base according to one-way ANOVA.Conclusion: Bay leaf extract is effective and potential to be used as toothpaste active agent for extrinsic stain removal on teeth and denture.
A histological evaluation of cellular response on bone regeneration scaffold Dewi, Anne Handrini; Listyarifah, Dyah; Ardhani, Retno; Jansen, John
Majalah Kedokteran Gigi Indonesia Vol 9, No 1 (2023): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77449

Abstract

Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.
Anti-inflammatory loading of cinnamaldehyde on artificial bone scaffolds against the process of bone regeneration Dewi, Anne Handrini; Triawan, Andi; Jansen, John
Majalah Kedokteran Gigi Indonesia Vol 9, No 3 (2023): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.87347

Abstract

Cinnamaldehyde is an organic component in cinnamon and has anti-inflammatory, antibacterial, and osteogenic properties. Loading cinnamaldehyde with a concentration of 4% into an artificial scaffold from gypsum calcium carbonate hydrogel could reduce inflammation, accelerate healing, and promote new bone regeneration at bone implantation surgery. The aim of this study was to investigate the impact of cinnamaldehyde loaded on gypsum-hydrogel calcium carbonate on the process of wound healing and bone regeneration in rats. Implantation of bone scaffolding from gypsum hydrogel calcium carbonate with an addition of cinnamaldehyde was performed on 20 5-month-old Sprague-Dawley rats weighing 300-350 g in an artificial defect of condyle femoris bone dexter and sinister. Group A was a control with gypsum only, while groups B, C, and D were given gypsum and CaCO3 with a combination of cinnamaldehyde or dehydrothermal treatment (DHT). Euthanasia was performed after implantation at 1, 4 and 8 weeks for 4 groups (n = 3). Femoris condyle bone was cut, made into histological preparations by hematoxylin eosin (HE) staining, and analyzed descriptively. The results showed that the loading of cinnamaldehyde on the scaffold was effective in weeks 1 and 4, but after 8 weeks of implantation, cinnamaldehyde was observed to inhibit defect closure. The cinnamaldehyde group combined with dehydrothermal (DHT) treatment was found to be better than those without DHT.
Co-Authors Andi Triawan Andi Triawan, Andi Anindya Prima Yusinta Archadian Nuryanti Asikin Nur Dyah Listyarifah Fajar Kumalasari Hendri Susanto Heni Susilowati Heni Susilowati Heribertus Dedy Kusuma Yulianto Ika Dewi Ana Ika Dewi Ana Indah Lestari Vidyahayati Intan Ruspita Intan Ruspita, Intan istiqomah, Yuli Westria Ivan Arie Wahyudi Ivan Arie Wahyudi Jansen, John Khong Mei Xuan Mayu Winnie Rachmawati Mutiara Annisa Nunuk Purwanti Priutami, Lyza Retno Ardhani Rifa, Rizky Steven Kumar Tasya Adistya Trianna Wahyu Utami Yuli Westria istiqomah Zipora Silka Yoretina