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All Journal HAYATI Journal of Biosciences BIOWALLACEA Jurnal Bioedukasi Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosel: Biologi Science and Education Jurnal Agritechno JURNAL BUDIDAYA PERTANIAN Agrotech Journal Rumphius Pattimura Biological Journal PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya
Adriana Hiariej, Adriana
Jurusan Biologi Fakultas MIPA Universitas Pattimura Ambon, Jl. Ir. M. Putuhena, Kampus Poka Ambon 97233 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Journal : Agrotech Journal

 1 
GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) Fuadiska Salamena; Adriana Hiariej; Cecilia Anna Seumahu
Agrotech Journal Vol 3, No 1 (2018): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v3i1.524

Abstract

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species
Genetic Characterization of Maize Kisar Var. Kuning Genjah and Maize Var. Bisi-II-Hibrida Based On Molekular RAPD Marker Elizabeth C. Berhitu; Adriana Hiariej; Cecilia A. Seumahu
Agrotech Journal Vol 4, No 2 (2019): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v4i2.1127

Abstract

The variety of maize in Southwest Maluku (MBD) has an ability to grow in the extremely dry land, especially at Kisar island, because those areas were dominated by dry land. Dry resistance very useful to be used for genetical characterization in order to provide accurate data about the character of local maize Kisar var. Kuning Genjah. To identify the characteristics at the genome level of this local maize variety, one of the molecular methods that can be applied was RAPD molecular method. The advantage of the RAPD marker is it's technically simpler and fast in testing, does not require DNA sequence information, hence this marker becomes widely used, it only requires a small sample of DNA, the primary is commercially available and does not use radioactive compounds. The aim of this study was to find out the bands profile that has the potential to be used as a marker of dry resistance. The method applied was RAPD (Random Amplified Polymorphic DNA) using OPA-02, OPA-07, and OPC-12 primers and produces 22 DNA bands. The measurement of bands by estimating the molecular weight based on marker exponential regression. The polymorphic percentage was 90.9% between var. Kuning genjah and var. BIZI-II-Hybrid. The percentage of polymorphism showed the potential of bands that can be used as molecular markers for markers of dry resistance that can be utilized in plant breeding
Co-Authors Aca A Anakotta Agus Sutanto Anneke Pesik Batlajery, Yulius Cecilia A. Seumahu CECILIA ANNA SEUMAHU Clemens Sudiyono Narahayaan Daro, Prichilia Dian Safitri Edizon Jambormias Elizabeth C. Berhitu FENNY MARTHA DWIVANY Fuadiska Salamena Gebiana Nyainleta Giasintha Stefani Husna Nugrahapraja I NYOMAN RAI Julian Timisela Katerina Jagagura Peilouw Ketut Wikantika Lamerkabel, Jacobus Suruka Aifaman Lapu, Petrus Martha Kaihena Masela, Efer Simon Nindatu, Maria Nisrina Sukriandi Owend Paul Letelay Patty, Kristi Lenci Pieter Agusthinus Riupassa Ridwan, La Rijal, Muhammad Riupassa, Pieter A. Rumpeniak, Yusuf Sahertian, Dece Elisabeth Sahubauwa, Laila Samputri, Salma Sulastri Sulastri Telussa, Ronald Topik Hidayat