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All Journal Jurnal Ilmu Pertanian Indonesia AL KAUNIYAH BIOWALLACEA Jurnal Penelitian Pendidikan IPA (JPPIPA) Bioma : Jurnal Biologi Indonesia Sarwahita : Jurnal Pengabdian Kepada Masyarakat JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia SABDAMAS Jurnal Al Azhar Indonesia Seri Ilmu Sosial Biosaintifika: Journal of Biology & Biology Education
Arief Pambudi
Program Studi Biologi, Fakultas Sains Dan Teknologi, Universitas Al-Azhar Indonesia, Jakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Public Health Social Sciences Other

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PEMANFAATAN TEKNIK HIDROPONIK ALTERNATIF BAGI RPTRA PONDOK KELAPA BERSERI Arief Pambudi; Nita Noriko; Yunus Effendi; Risa Swandari Wijihastuti
Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia Vol 1, No 1 (2019)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/jpm.v1i1.332

Abstract

AbstrakSalah satu masalah produksi pangan di Indonesia khususnya di daerah perkotaan adalah terbatasnya area pertanian. Hidroponik dapat menjadi satu solusi untuk masalah ini. Hidroponik umumnya menggunakan formula nutrisi AB-Mix yang terkadang masyarakat sulit mendapatkannya. Bahan alternatif yang memiliki potensi sebagai sumber nutrisi hidroponik adalah air sisa pencucian beras. Ruang publik terpadu ramah anak (RPTRA) Pondok Kelapa Berseri merupakan suatu komunitas yang dapat direkomedasikan untuk produksi sayuran daerah urban khususnya di wilayah Pondok Kelapa, Jakarta Timur. Hasil kegiatan menunjukkan bahwa penggunaan air beras sebagai medium hidroponik memiliki potensi untuk dikembangkan walaupun penambahan konsentrasi air beras masih perlu dilakukan. Sosialisasi dan sharing pengalaman dengan masyarakat dilakukan pada 50 peserta sekitar RPTRA dan mendapat respon yang baik. Pengurus RPTRA berharap kegiatan seperti ini dilakukan periodik dan disinkronkan dengan jadwal kegiatan PKK di RPTRA Pondok Kelapa lebih banyak orang mendapatkan manfaatnya.Kata kunci: Hidroponik, Media Tumbuh Hidroponik, Media Alternatif Hidroponik Abstract One problem of food production in Indonesia, especially in urban areas is the limited area of agriculture. Hydroponics can be a solution to this problem. Hydroponics generally uses the AB-Mix nutritional formula, which is sometimes difficult to get. Alternative material that potential as a hydroponic nutrient source is residual rice washing water. RPTRA Pondok Kelapa Berseri is a community that can be  recommended to vegetable production in urban area, especially in the Pondok Kelapa, East Jakarta. The results of the activity indicate that the use of rice water as a hydroponic medium has the potential to be developed even though additional concentrations of rice water still need to be done. Socialization and sharing experiences with the community were carried out on 50 participants around RPTRA and received good responses. RPTRA hopes that activities like this will be conducted periodically and synchronized with the PKK schedule at RPTRA Pondok Kelapa, so more people will get the benefits.Keywords: Hydroponic, Hydroponic Medium, Hydroponic Alternative Medium
Hidroponik Sederhana Bagi Komunitas Anak Jalanan Riris Lindiawati Puspitasari; Arief Pambudi; Yunus Effendi
Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia Vol 4, No 3 (2022)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/jpm.v4i3.965

Abstract

Keterbatasan sarana pembelajaran sains terapan, perlunya ketrampilan urban farming, keterbatasan media pembelajaran, dan perlunya pembekalan jiwa entrepreneur menjadi pendorong pelaksanaan kegiatan abdimas peningkatan ketrampilan komunitas anak jalanan di Depok melalui hidroponik sederhana. Peningkatan ketrampilan berhidroponik diharapkan mitra dapat mempraktekkan, memanfaatkan sayuran hasil panen hidroponik untuk kebutuhan keluarga dan menghasilkan pendapatan ketika menjual hasil sayurannya. Metode pelatihan yang dilakukan yaitu dengan mengadakan pelatihan bagi anak jalanan peserta didik di Sekolah Master Depok, mempraktekkan secara langsung hidroponik sayuran menggunakan peralatan sederhana, dan membuat kelompok untuk pendampingan. Peserta sosialisasi berasal dari warga sekitar sekolah dan sebagian peserta didik dari tingkat sekolah menengah pertama. Hasil yang diperoleh adalah peserta mendapat informasi teknik hidroponik menggunakan peralatan sederhana. Kegiatan berikutnya adalah melatih secara langsung dan mendampingi praktek hidroponik sederhana menggunakan sistem Wicks (sumbu). Sayuran yang ditanam antara lain kangkung, bayam, sawi, dan pakcoy. Keberhasilan pelatihan terlihat dari pertumbuhan sayuran yang tidak terhambat hingga panen (usia 8 minggu setelah tanam). Pendampingan yang dilaksanakan adalah pendampingan praktek secara online melalui aplikasi komunikasi dan secara langsung di sekolah. Mitra mendapatkan pengetahuan dan ketrampilan tentang hidroponik sayuran sederhana selama kegiatan berlangsung.Kata kunci: Hidroponik, Pemberdayaan, sistem Wicks, Teknologi Tepat Guna
Soil Conservation in Brassica rapa chinensis L (Pakcoy) Growth Nita Noriko; Rachmad Agustono; Risa Swandari Wijihastuti; Elya Novani; Nadiyah Isna Choirunnisa; Arief Pambudi
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6989

Abstract

Population increase has an impact on the procurement of food sources. Pakcoy is a vegetable that is in increasing demand. Farmers' response to this condition is to use excessive fertilizer and pesticides, thereby reducing soil fertility. The research aims to obtain a combination of fertilizer and humic acid, which is effective in cultivating B. rappa chinesis L., as well as soil conservation efforts. The methodology was experimental with Randomized Block Design (RBD) on four treatments, K, P1, P2, and P3. The dose of K was 150 g/5 m2, NPK 16:16:16, 1 kg/5 m2 dolomite, and 5 kg/5 m2 manure. The P1 treatment is the same as K but without NPK fertilizer. P2 and P3 treatments were reduced by 50% and 70% of NPK fertilizer, respectively, with the addition of humic acid. The research results show that P2 with local environmental conditions provides optimal results in terms of chlorophyll, stomata, and plant height. Soil fertility was shown by increasing pH to 6.6, K 281 ppm and P 509 ppm, Cation Exchange Capacity (CEC) 31.60 cmolc/kg, soil enzymes of 0.12 mg FDA g-1 soil d.w. h-1, an average number of earthworms of 17. The conclusion is that P2 fertilizer formulation can be applied to cultures as an anticipation of food security.
Studies on the Short- and Long-Term Effects of Rubber-Canna Agroforestry Through Soil Analysis and a Metagenomic Approach Sasaerila, Hidayat Yorianta; Effendi, Yunus; Wijihastuti, Risa Swandari; Pambudi, Arief; Nicola, Flavia De
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.3514

Abstract

Agroforestry combines trees and crops for sustainable benefits. We explore rubber and canna integration into agroforestry, emphasizing sustainability, biodiversity, and carbon sequestration. This study assesses C. indica's viability beneath 7-8-year-old rubber plantations, examining its impact on soil, microbial communities, and latex production. The research site in Subang, Indonesia, features, at the beginning, six-year-old rubber trees with variations in sunlight under canopies. Wild C. indica rhizomes from Mid Java are planted beneath rubber trees and open ground. No significant difference was found in plant height, rhizome weight, leaf area, number of leaves, r/s ratio, SLA, LWR, and LAR between C. indica cultivated beneath rubber trees (RC agroforestry) and on open ground. Although not significant, RC soil had higher N, P, K, and organic C levels than rubber monoculture (RM) soil two years after adopting the rubber-canna agroforestry system. After six years, RC soil had a greater pH, C, N, P, and K, clay and silt content,   and Shannon E index than RM soil. Analysis of soil metagenomics showed the phylum Proteobacteria dominates and enhances soil fertility, particularly in RC soils. These results increase latex output at the RC site over the RM location. In conclusion, the  Rubber-Canna agroforestry system enhances sustainability, soil fertility, and crop yield, addressing food security and environmental concerns. The primary novelty of this six-year study lies in the integration of C. indica into Southeast Asia's rubber agroforestry systems, highlighting its unique characteristics such as low-light survival, which can contribute to food security and soil protection.
ANALISIS VEGETASI Ficus Racemosa L. DI BANTARAN SUNGAI CILIWUNG WILAYAH PANGADEGAN JAKARTA SELATAN: Analysis Vegetation Of Ficus Racemosa L In The Ciliwung District Of Pangadegan South Jakarta Zaharah, Puteri; Noriko, Nita; Pambudi, Arief
Bioma Vol. 12 No. 2 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.806 KB) | DOI: 10.21009/Bioma12(2).2

Abstract

River is the most important things for the human life, one of the components environmental especially for whom whose living in the city and Ciliwung river is one of the major river beside of others 13 rivers. Bamboo trees, bananas, cotton, gempol and elo are the common flora were dominate plants along the riverbanks (gempol and elo taken from the local people words). Based from interview with some local citizens in the Ciliwung riverbank which pas through the district of Pangadegan (South Jakarta), Ficus racemosa L (elo tree) is the most important plant which can withstand river erosion and could maintained the riverbanks during flood and continued by taking sample that plant on that district for need of analysis and identification. From the calculation Gigantochloa apus types stake has the highest INP value to the over all plot of 65.43% while INP Ficus racemosa is still low, but Ficus racemosa can be one alternative plant for conservation in the Ciliwung river, because Ficus racemosa has strong roots. However the selection of other plants such as bamboo trees is more effective because it has fast growth. Therefore, by combining the types of plants that role as conservation may be a solution.
ANALISIS BAKTERI Escherichia coli PADA MAKANAN SIAP SAJI DI KANTIN RUMAH SAKIT X DAN KANTIN RUMAH SAKIT Y: Analysis of Escherichia coli Bacteria on Fast Food at hospital X and Y cafetaria Saridewi, Inggit; Pambudi, Arief; Ningrum, Yulia Fitria
Bioma Vol. 12 No. 2 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (852.785 KB) | DOI: 10.21009/Bioma12(2).4

Abstract

The increasing human activity makes the preference for fast food increases. However, some people do not pay attention to the hygiene conditions of food processed from food stalls. Food handlers, equipment utilization, food processing, clean water, and the packaging are the critical points of bacterial contamination. Escherichia coli is a bacterium that usually used as the indicator of food hygiene. The objective of this study is to examine the contamination of coliform bacteria, especially E. coli at two hospital cafeteria by using MPN method and questionnaire regarding the implementation of the basic principles of hygiene. Stages of tests performed that are the presumption test, confirmation test, complementary test, gram stain test, biochemical test IMViC and supported by a questionnaire. From the two locations tested, some samples showed positive result in a presumption test and confirmation test but negatively complementary to biochemical test. This indicates that the sample does not contain E. coli bacteria in food, but there is the possibility of Citrobacter. The negative results of the IMVIC test showed that it is possible bacteria found in the presumption test and confirmation test not E. coli and non-pathogenic bacteria. Based on the results of the questionnaire, most of restaurant owner has understood to served food. Food at the hospital X and Y cafetaria are safe to consume because it has a negative E.coli.
Pemberdayaan Kemitraan Masyarakat Petani: Penerapan Multi Cropping dan Smart Farming di Dusun Cihieum, Desa Sukanagalih Cianjur, Jawa Barat Noriko, Nita; Effendi, Yunus; Pambudi, Arief; Arniaty, Andi; Armelia, Adela; Mandjusri, Alma
Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia Vol 7, No 1 (2024): Desember 2024
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/jpm.v7i1.3211

Abstract

Biaya  produksi yang tinggi dibandingkan dengan penjualan merupakan masalah yang dihadapi petani dusun Cihieum Kampung Cibeureum, desa Sukanagalih. Penyebabnya adalah biaya sewa lahan, kebutuhan pupuk, pestisida, ketersediaan air dan upah tenaga kerja untuk pengolahan lahan. Solusi dalam menghadapi permasalahan ini adalah menerapkan, multi cropping, smart farming, konservasi tanah dan air serta penggunaan traktor tangan. Tujuan yang akan dicapai adalah mengurangi biaya produksi dan meningkatkan hasil pertanian. Metode yang dilakukan untuk menjalankan solusi tersebut adalah mengadakan Pemberdayaan Kemitraan Masyarakat(PKM) untuk petani mitra melalui sosialisasi dan workshop. Hasil PKM menunjukkan adanya peningkatkan pengetahuan dan kemampuan petani untuk melaksanakan multi cropping dan smart farming, konservasi tanah dan air serta penggunaan traktor tangan. Penerapkan multi cropping dan smart farming, konservasi tanah dan air serta traktor tangan dapat menekan biaya produksi hingga 30% dan hasil pertanian pada satu areal terdiri atas 2 komoditi seperti Capsicum annuum (cabai merah) dan Phaseolus vulgaris (buncis). Hasil produksi C. annuum dapat meningkat 30% dan P. vulgaris 100%. Peningkatan keuntungan bududaya cabai merah  dapat mencapai 91%.
Molecular and Growth Responses of Musa acuminata var. Barangan Post Application of Beneficial Endophytic Bacteria Hanifah Yusadi; Arief Pambudi; Yunus Effendi
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.40

Abstract

Endophytic bacteria reside in plants' roots and can benefit plant growth. The study aimed to evaluate the application of an endophytic bacterial consortium in enhancing the growth of banana plants and identify genes that maximally assist in nutrient utilization during banana plant growth. One-month-old banana plants were inoculated by soaking them for one hour in a 500 mL suspension of endophytic bacteria. In vivo observations were conducted in the greenhouse at Al-Azhar University Indonesia over 40 days, during which morphological and physiological growth were calculated. The results revealed that control plants exhibited lower growth than those treated with endophytic bacteria. The application of endophytic bacteria led to increased growth in banana barangan plants, as evidenced by improvements in leaf length, leaf width, plant height, and chlorophyll levels. Furthermore, a semi-quantitative analysis of banana plants treated with Cytobacillus depressus, Bacillus stratophericus, and B. mycoides revealed genes significantly contributing to growth. These genes, including WRKY33, Ma03_92660, Ma01_901890, Ma04_936790, and Pho-1,2, exhibited their highest expression levels starting from the 28th day of the experiment. Keywords: plant growth, endophytic consortium bacteria, semi-quantitative real-time PCR, identify genes
Optimization of RNA Extraction Method of Banana Leaf Using Li Buffer and GENEzol Reagent Kusumaningtyas, Ida Ayu Mas Amelia; Pambudi, Arief; Effendi, Yunus
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 10, No 2 (2025): Mei 2025
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v10i2.2092

Abstract

High quality extracted RNA from plant tissues is used in downstream transcriptomic application that needs high quantity for multiple analysis. Attaining high quality and high yield for banana plant tissue is a challenge with its high number of secondary metabolites becoming contaminants, effecting the purity of RNA extracted. A procedure to extract banana plant RNA with high quality and quantity in this study is developed to be simple, robust, affordable and accessible using basic lab equipment and materials. This study optimizes RNA extraction from the leaf of juvenile Pisang Susu (Musa spp.) using a combination of Li buffer (modified CTAB) and GENEzol reagent. Treatment differs in the use of β-mercaptoethanol and the duration of sample precipitation with isopropanol. NucleoSpin RNA Plant extraction kit was made as comparison. Measurement of RNA quantity used quantus fluorometer, and quality measured by spectrophotometer. Results showed that the addition of β-mercaptoethanol in Li buffer is vital with samples left to precipitate overnight providing best results. The total RNA obtained had a higher yield compared to the commercial kit with 108 – 211 ng/µl and 0.35 – 0.37 ng/µl respectively. Light absorbance A260/280 indicating sample purity between method has similar RNA quality of 0.917 – 1.084 and 0.843 – 1.026. This study proved that modification of CTAB buffer using Li buffer for RNA extraction resulted in better quality and quantity of RNA compared to the commercial RNA extraction kit.Keywords – Banana, Optimization, RNA Extraction.
Co-Authors Adinda Citra Dianita Ainul Haq Analekta Tiara Perdana Armelia, Adela Arniaty, Andi Basma Basma Dewi Elfidasari Elya Novani Endah Permata Sari Firman Alamsyah Hanifah Yusadi Hidayat Yorianta Sasaerila Kusumaningtyas, Ida Ayu Mas Amelia Mandjusri, Alma Nadiyah Isna Choirunnisa Nicola, Flavia De Ningrum, Yulia Fitria Nita Noriko Nurul Fauzia Purwanty Rara Azura Putri Kenanga Rachmad Agustono Riris L. Puspitasari Riris Lindiawati Puspitasari Risa Azhari Rossi Septy Wahyuni Saridewi, Inggit Susanti Susanti Syaefudin - Taufiq W Priambodo Taufiq Wisnu Priambodo Wijihastuti, Risa Swandari Wijihastuti, Risa Swandari Yunus Effendi Zaharah, Puteri