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GENETIC DIVERSITY AMONG SUGARCANE (Saccharum officinarum L.) GENOTYPES AS SHOWN BY RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) Dwi Hapsoro; Hayane Adeline Warganegara; Setyo Dwi Utomo; Nanik Sriyani; Yusnita Yusnita
AGRIVITA, Journal of Agricultural Science Vol 37, No 3 (2015): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i3.499

This experiment was conducted to reveal genetic diversity among 38 genotypes of sugarcane (Saccharum officinarum L.) using RAPD markers. The population consisted of 8 genotypes from Australia, 7 from Africa, 10 from America, and 13 from Asia. Genetic similarity was ranging from 17% to 97% , with the average of 57%. UPGMA dendrograms divided the population into three major groups i.e. group 1, 2, and 3 which consist-ed of 23, 10, and 5 genotypes, respectively. Each major group comprised genotypes of different geographical origins. The dendrogram divided each group into some subgroups. There were 8 subgroups i.e. 4 subgroups in group 1, 2 sub-groups in group 2, and 2 subgroups in group 3. Some genotypes of same geographical origin were clustered into in at least 3 different sub-groups, meaning that they were genetically dis-similar. On the other hand, some other geno-types of different geographical origin were clustered into the same subgroup, meaning that they were genetically similar. This data would help sugarcane breeders to select parents for hybridization in order to maximize heterosis. This could be conducted by selecting parents of dis-similar genotypes.

The growth of 'Crystal' guava seedling in response to pinching and dormancy breaking chemicals Raden Ajeng Diana Widyastuti; Setyo Dwi Utomo; Darwin H. Pangaribuan; Purba Sanjaya; Hayane Adeline Warganegara; Widia Agustin
Kultivasi Vol 21, No 3 (2022): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v21i3.40791

AbstractPinching and dormancy breaking chemicals (DBC) application are potentially used to regulate the growth of plant. This study aims to evaluate the growth response of ‘Crystal’ guava (Psidium guajava L.) seedling in response to pinching and DBC application. This experiment was carried out in the Integrated Field Laboratory, Faculty of Agriculture, Universitas Lampung from March to June 2021, with a randomized completely block design (RCBD) with 2 factors (pinching and DBC) and repeated four times. The results showed that pinching could reduce the increase of height of guava seedling. The combination of pinching with DBC could significantly increase the number of new emerging leaves, branches, and shoots as well as the length of new shoots. The leaf area on new emerging leaves was not affected by pinching and DBC factors. The application of DBC to non-pinched plants inhibited vegetative growth and precisely increased generative growth, as indicated by the increase of the number of flowers produced. The most recommended treatment to improve the vegetative growth of guava plant seedlings was a combination of pinching and KNO3 40 g L-1.Keywords: 'Crystal' guava, KNO3, vegetative growth, pinching, dormancy breaking chemicals. AbstrakPinching dan pemberian zat pemecah dormansi berpotensi untuk digunakan sebagai pengatur pertumbuhan tanaman. Penelitian ini bertujuan untuk mengevalusi respon pertumbuhan bibit tanaman jambu biji (Psidium guajava L.) 'Kristal' terhadap perlakuan pinching dan pemberian zat pemecah dormansi (ZPD). Percobaan ini dilaksanakan di lahan Laboratorium Lapang Terpadu, Fakultas Pertanian, Universitas Lampung dari bulan Maret hingga Juni 2021, dengan rancangan acak kelompok (RAK) faktorial (pinching dan ZPD) yang diulang sebanyak 4 kali. Hasil penelitian ini menunjukan bahwa teknik pinching dapat menurunkan pertambahan tinggi tanaman jambu. Namun hal ini justru dapat memperbaiki pertumbuhan bibit jambu. Kombinasi pinching dengan ZPD dapat meningkatkan jumlah daun, cabang, dan tunas baru serta panjang tunas baru secara signifikan. Luas daun baru tanaman jambu tidak dipengaruhi oleh faktor pinching dan ZPD. Pemberian ZPD pada tanaman yang tidak dipinching menghambat pertumbuhan vegetatif, sebaliknya meningkatkan pertumbuhaan generatif dengan indikator peningkatan jumlah bunga yang diproduksi. Perlakuan yang paling direkomendasikan untuk memperbaiki pertumbuhan vegetatif bibit tanaman jambu adalah kombinasi antara pinching dan KNO3 40 g L-1.Kata kunci: jambu biji ‘Kristal’, KNO3, pertumbuhan vegetatif, pinching, zat pemecah dormansi

Pengaruh Metode Aplikasi dan Konsentrasi Gibberellic Acid (GA3) terhadap Fase Pertumbuhan Vegetatif Tanaman Nanas (Ananas comosus [L.] Merr) Maraaini, Ajeng; Widyastuti, R. A. Diana; Warganegara, Hayane Adeline; Karyanto, Agus
JURNAL AGROTROPIKA Vol 23, No 1 (2024): Jurnal Agrotropika Vol 23 No 1, Mei 2024
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v23i1.7698

Increasing the production of pineapple fruit can be done by manipulating the plant using Gibberellic Acid Growth Regulatory Substance (ZPT). The purpose of this study was to determine the effect of GA3 concentration and application method, as well as its interaction in increasing the vegetative growth of pineapple plants. Data collection was carried out in November 2022- February 2023 at a pineapple plantation company in Central Lampung. Treatments were arranged factorial (3x2) using a randomized block design with 3 replications. The first factor was Gibberellin (A) which consisted of three levels, (A1) 0 ppm GA3, (A2) 200 ppm GA3, and (A3) 400 ppm GA3. The second factor is the application method (B) which consists of 2 levels, namely (B1) Application on the top of the leaf (B2) Application on the bottom of the leaf. The observed variables included plant height, stem diameter, number of leaves, D-leaf length, D-leaf width, number of roots, root length, fresh weight of roots, fresh weight of plants. Homogeneity of variance between treatments was tested by Barlett's test and additivity using Tukey's test and then analysis of variance was carried out. Separation of the mean was carried out using the least significant difference test (LSD) at the 5% level. The results showed that 400 ppm GA3 was better than the control in affecting plant fresh weight with a difference of 630,7 g, the treatment of the application method on the leaves was better than the bottom leaf in affecting plant fresh weight with a difference 201,4 g. Treatment with a concentration of 40 ppm using the leaf application method was better than the control treatment. It is suggested to do further research related to the effect of giving GA3 on the generative phase of pineapple plants.Keywords : Gibberellic Acid, application method, pinneaple

Teknologi Pemupukan Tanaman Kopi dengan Penambahan Pupuk Organik di Desa Margoyoso, Kecamatan Sumberejo, Kabupaten Tanggamus Sanjaya, Purba; Ramadiana, Sri; Widyastututi, RA Diana; Pujisiswanto, Hidayat; Warganegara, Hayane Adeline
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3, No 2 (2024): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3 No 2, September 2
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v3i2.9825

Provinsi Lampung, sebagai salah satu penghasil kopi terbesar di Indonesia, menghadapi tantangan serius terkait rendahnya produktivitas kopi. Dengan rata-rata produksi hanya mencapai 0,75 ton per hektar, jauh di bawah potensi genetiknya yang mencapai 4 ton per hektar, sejumlah masalah budidaya menjadi penyebab utama rendahnya hasil panen. Salah satu masalah yang signifikan adalah kurangnya pemupukan tanaman dan penggunaan pupuk yang kurang lengkap oleh para petani. Sentra kopi di Provinsi Lampung, seperti Kabupaten Lampung Barat dan Tanggamus, menunjukkan perbedaan signifikan dalam produktivitas kopi. Kabupaten Lampung Barat memiliki produktivitas kopi yang lebih tinggi, mencapai 1,07 ton per hektar, sementara Kabupaten Tanggamus hanya mencapai 0,8 ton per hektar. Oleh karena itu, meningkatkan produktivitas kopi di Kabupaten Tanggamus menjadi kunci untuk mengangkat produktivitas secara keseluruhan di Provinsi Lampung.Berdasarkan tingkat kesuburan tanah, khususnya di Kecamatan Sumber Rejo tergolong rendah sampai sangat rendah. Oleh sebab itu, dalam pelaksanaan budidaya kopi, sangat direkomendasikan untuk melaksanakan kegiatan pemupukan dengan memperhatikan Teknik pemupukan yang tepat, selain itu perlu ditambahkan pembenah tanah seperti pupuk organik dan biochar. Hasil dari pengabdian ini adalah bawha pelaksanaan pengabdian mampu meningkatkan pengetahuan petani terkait budidaya kopi dari 42,6% Menjadi 87,6%. Selain itu, petani juga mampu melaksanakan pembuatan pupuk organik padat secara mandiri mulai dari pemilihan bahan, pembuatan larutan dekomposer, pembuatan lapisan hingga pengecekan suhu kompos dengan baik dan benar. Pengabdian ini diharapkan dapat meningkatkan produktivitas tanaman kopi dan kesejahteraan petani sekitar.

PENGARUH PEMBERIAN DOSIS PUPUK NPK DAN PUPUK HAYATI TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN CABAI MERAH (Capsicum annuum L.) R.A Diana Widyastuti; Yohanes C Ginting; Purba Sanjaya; Hayane Adeline Warganegara; M Hafidz Yugo
Jurnal Agrotek Tropika Vol 10, No 3 (2022): JURNAL AGROTEK TROPIKA VOL 10, AGUSTUS 2022
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v10i3.6304

Produktivitas cabai merah mengalami penurunan setiap tahunnya hal ini disebabkan oleh kesuburan tanah yang semakin rendah. Upaya yang dapat dilakukan untuk mengatasi permasalahan tersebut yaitu melakukan pemupukan dengan pupuk NPK dan pupuk hayati. Penelitian ini dilaksanakan di Desa Sukabanjar, Gedong Tataan, Pesawaran padai bulan November 2020 sampai April 2021. Metode penelitian yang digunakan yaitu Rancangan Acak Kelompok (RAK) faktorial 4x3 dengan 3 ulangan dan terdapat 12 kombinasi perlakuan. Faktor pertama yaitu perlakuan dosis pupuk NPK 0, 10, 20, dan 30 g/tanaman. Faktor kedua yaitu konsentrasi pupuk hayati Grikulan Plus 0, 15, 30 ml/l. Setiap perlakuan diambil 2 sampel. Homogenitas ragam diuji dengan uji Bartlett dan aditivitas menggunakan uji Tukey, dan uji lanjut dengan BNT padai taraf 5%. Hasil penelitian menunjukkan perlakuan dosis pupuk NPK 30 g/tanaman menghasilkan bobot buah cabai merah paling tinggi yaitu sebesar 521,44 g/tanaman. Pengaplikasian pupuk hayati Grikulan Plus konsentrasi 30 ml/l menghasilkan bobot cabai merah yang lebih tinggi sebesar 415,21 g/tanaman. Interaksi antara dosis pupuk NPK 30 g/tanaman dengan pupuk hayati Grikulan Plus konsentras 30 ml/l menghasilkan bobot cabai merah yang tertinggi yaitu sebesar 657,67g/tanaman atau setara dengan 13,15 ton/ha.

PEMBUNGAAN TANAMAN SPATIFILUM (Spathiphyllum wallisii Regel) AKIBAT PENGARUH RESIDU PEMBERIAN BENZILADENIN (BA) PADA PERIODE KEDUA Rugayah, Rugayah; Warganegara, Hayane Adeline; Karyanto, Agus; Ardanti, Ade Novia
JURNAL AGROTROPIKA Vol 24, No 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9675

Spatifilum is classified as a shade plant that can survive in environments with minimal sunlight or shading Spatifilum plants are known to absorb toxins or clean indoor air from pollutants such as benzene and formaldehyde. Efforts to stimulate flowering and the emergence of spatifilum seedlings include using a growth regulator in the form of benzyladenine (BA). The aim of this research was to determine the difference in the effect of residual BA with and without BA on the flowering of spatifilum plants in the second period. Data collection was carried out in August – November 2022 at the Horticulture Greenhouse, Faculty of Agriculture, University of Lampung. This research used a Complete Randomized Block Design (RAKL) with a single treatment. The treatment consisted of 5 BA concentration treatments, namely 0 ppm, 20 ppm two applications, 20 ppm four applications, 40 ppm two applications, and 40 ppm four applications with four repetitions. The data from this research were analyzed using the barlet test and the additivity test was carried out using the Tukey test. Then the data was analyzed using variance (Anara) and orthogonal contrast tests. The results of the research showed that there were significant differences in the variables of increasing the number of seedlings and increasing the number of flowers. BA concentration of 20 ppm was significantly able to increase the number of offspring compared to a concentration of 40 ppm, meanwhile, a concentration of 40 ppm with a frequency of giving 2x significantly increased the number of flowers compared to a frequency of giving 4x

Percontohan Penerapan Teknologi Bujangseta pada Pertanaman Jeruk di Pekon Giham Sukamaju, Kecamatan Sekincau Lampung Barat Rugayah, Rugayah; Karyanto, Agus; Sembodo, Dad; Warganegara, Hayane Adeline
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 4, No 1 (2025): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 4 No 1, Maret 2025
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v4i1.10788

Pekon Giham Sukamaju termasuk dalam wilayah dataran tinggi di Kecamatan Sekincau Lampung Barat yang cocok untuk usaha pertanian, khususnya tanaman hortikultura. Beberapa petani di Pekon Giham, Sukamaju mulai tertarik menanam jeruk sebagai komoditas unggulan baru, tetapi pemahaman dalam budidaya jeruk masih minim dan belum sesuai dengan kaidah GAP (good agricultural practices) untuk dapat menghasilkan panenan yang sehat, bermutu tinggi, dan berdaya saing sehingga mampu meningkatkan pendapatan petani. Pengamatan di lapang menunjukkan bahwa petani menanam jeruk seadanya terutama dalam pemeliharaan tanaman jeruk meliputi pemupukan dan pemangkasan. Tujuan kegiatan ini adalah (1) memberikan pengetahuan teoritis tentang teknik budidaya jeruk di dataran tinggi menurut kaidah GAP, (2) agar petani mengetahui dan mampu menerapkan teknologi bujangseta pada pertanaman jeruk. Hasil kegiatan menunjukkan bahwa terjadi peningkatan pengetahuan pemeliharaan jeruk dengan penerapan teknologi bujangseta sebesar 43,3%, terutama dalam hal pemangkasan dan pemupukan. Pemahaman dalam praktik penerapan teknologi bujangseta hanya terlihat pada lima orang peserta dari 13 anggota petani jeruk. Ketrampilan teknis budidaya jeruk yang dikuasai terutama pemupukan dan sebagian pemangkasan. Manajemen pengendalian OPT kurang dipaham petani.

Pengaruh Metode Aplikasi dan Konsentrasi Gibberellic Acid (GA3) terhadap Fase Pertumbuhan Vegetatif Tanaman Nanas (Ananas comosus [L.] Merr) Maraaini, Ajeng; Widyastuti, R. A. Diana; Warganegara, Hayane Adeline; Karyanto, Agus
JURNAL AGROTROPIKA Vol. 23 No. 1 (2024): Jurnal Agrotropika Vol 23 No 1, Mei 2024
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v23i1.7698

Increasing the production of pineapple fruit can be done by manipulating the plant using Gibberellic Acid Growth Regulatory Substance (ZPT). The purpose of this study was to determine the effect of GA3 concentration and application method, as well as its interaction in increasing the vegetative growth of pineapple plants. Data collection was carried out in November 2022- February 2023 at a pineapple plantation company in Central Lampung. Treatments were arranged factorial (3x2) using a randomized block design with 3 replications. The first factor was Gibberellin (A) which consisted of three levels, (A1) 0 ppm GA3, (A2) 200 ppm GA3, and (A3) 400 ppm GA3. The second factor is the application method (B) which consists of 2 levels, namely (B1) Application on the top of the leaf (B2) Application on the bottom of the leaf. The observed variables included plant height, stem diameter, number of leaves, D-leaf length, D-leaf width, number of roots, root length, fresh weight of roots, fresh weight of plants. Homogeneity of variance between treatments was tested by Barlett's test and additivity using Tukey's test and then analysis of variance was carried out. Separation of the mean was carried out using the least significant difference test (LSD) at the 5% level. The results showed that 400 ppm GA3 was better than the control in affecting plant fresh weight with a difference of 630,7 g, the treatment of the application method on the leaves was better than the bottom leaf in affecting plant fresh weight with a difference 201,4 g. Treatment with a concentration of 40 ppm using the leaf application method was better than the control treatment. It is suggested to do further research related to the effect of giving GA3 on the generative phase of pineapple plants.Keywords : Gibberellic Acid, application method, pinneaple

PEMBUNGAAN TANAMAN SPATIFILUM (Spathiphyllum wallisii Regel) AKIBAT PENGARUH RESIDU PEMBERIAN BENZILADENIN (BA) PADA PERIODE KEDUA Rugayah, Rugayah; Warganegara, Hayane Adeline; Karyanto, Agus; Ardanti, Ade Novia
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9675

Spatifilum is classified as a shade plant that can survive in environments with minimal sunlight or shading Spatifilum plants are known to absorb toxins or clean indoor air from pollutants such as benzene and formaldehyde. Efforts to stimulate flowering and the emergence of spatifilum seedlings include using a growth regulator in the form of benzyladenine (BA). The aim of this research was to determine the difference in the effect of residual BA with and without BA on the flowering of spatifilum plants in the second period. Data collection was carried out in August – November 2022 at the Horticulture Greenhouse, Faculty of Agriculture, University of Lampung. This research used a Complete Randomized Block Design (RAKL) with a single treatment. The treatment consisted of 5 BA concentration treatments, namely 0 ppm, 20 ppm two applications, 20 ppm four applications, 40 ppm two applications, and 40 ppm four applications with four repetitions. The data from this research were analyzed using the barlet test and the additivity test was carried out using the Tukey test. Then the data was analyzed using variance (Anara) and orthogonal contrast tests. The results of the research showed that there were significant differences in the variables of increasing the number of seedlings and increasing the number of flowers. BA concentration of 20 ppm was significantly able to increase the number of offspring compared to a concentration of 40 ppm, meanwhile, a concentration of 40 ppm with a frequency of giving 2x significantly increased the number of flowers compared to a frequency of giving 4x

Teknologi Pemupukan Tanaman Kopi dengan Penambahan Pupuk Organik di Desa Margoyoso, Kecamatan Sumberejo, Kabupaten Tanggamus Sanjaya, Purba; Ramadiana, Sri; Widyastututi, RA Diana; Pujisiswanto, Hidayat; Warganegara, Hayane Adeline
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol. 3 No. 2 (2024): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3 No 2, September
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v3i2.9825

Provinsi Lampung, sebagai salah satu penghasil kopi terbesar di Indonesia, menghadapi tantangan serius terkait rendahnya produktivitas kopi. Dengan rata-rata produksi hanya mencapai 0,75 ton per hektar, jauh di bawah potensi genetiknya yang mencapai 4 ton per hektar, sejumlah masalah budidaya menjadi penyebab utama rendahnya hasil panen. Salah satu masalah yang signifikan adalah kurangnya pemupukan tanaman dan penggunaan pupuk yang kurang lengkap oleh para petani. Sentra kopi di Provinsi Lampung, seperti Kabupaten Lampung Barat dan Tanggamus, menunjukkan perbedaan signifikan dalam produktivitas kopi. Kabupaten Lampung Barat memiliki produktivitas kopi yang lebih tinggi, mencapai 1,07 ton per hektar, sementara Kabupaten Tanggamus hanya mencapai 0,8 ton per hektar. Oleh karena itu, meningkatkan produktivitas kopi di Kabupaten Tanggamus menjadi kunci untuk mengangkat produktivitas secara keseluruhan di Provinsi Lampung.Berdasarkan tingkat kesuburan tanah, khususnya di Kecamatan Sumber Rejo tergolong rendah sampai sangat rendah. Oleh sebab itu, dalam pelaksanaan budidaya kopi, sangat direkomendasikan untuk melaksanakan kegiatan pemupukan dengan memperhatikan Teknik pemupukan yang tepat, selain itu perlu ditambahkan pembenah tanah seperti pupuk organik dan biochar. Hasil dari pengabdian ini adalah bawha pelaksanaan pengabdian mampu meningkatkan pengetahuan petani terkait budidaya kopi dari 42,6% Menjadi 87,6%. Selain itu, petani juga mampu melaksanakan pembuatan pupuk organik padat secara mandiri mulai dari pemilihan bahan, pembuatan larutan dekomposer, pembuatan lapisan hingga pengecekan suhu kompos dengan baik dan benar. Pengabdian ini diharapkan dapat meningkatkan produktivitas tanaman kopi dan kesejahteraan petani sekitar.

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