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PERAN PROTEIN MEMBRAN LUAR 55 kDa Salmonella typhi ISOLAT JEMBER SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Candra Bumi; Heni Fatmawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.866 KB) | DOI: 10.23869/234

Abstract

Salmonella typhi is an obligate pathogen that is usually found in clinical specimen from typhoid fever patients. The pathogenic mechanism of bacteria are not fully elucidated especially its potential activity of the outer membrane protein (OMP) as hemaglutinin and adhesion molecule. After identification, bacterial isolate of outer membrane protein fraction 12,5% SDS-PAGE were used to isolate OMP followed by hemaglutinin test and in vitro adhesion test. The study showed that the 55 kDa protein of S. typhi is a hemaglutinin protein that could agglutinate, Wistar mouse erythrocytes. The 55 kDa OMP is also an adhesion protein showed by its activity to adherence to Wistar mice’s enterocyt. The increased dose of 55 kDa OMP will decrease the amount of S. typhi bacteria adherenced to Wistar mice’s enterocyt. Outer membrane protein 55 kDa S. typhi as hemagglutinin and also adhesion protein.
Hubungan antara intensitas Pruritus Nokturna dengan Kualitas Tidur pada Penderita Skabies Ghani Silahudin; Erma Sulistyaningsih; Heni Fatmawati
Majalah Kedokteran Andalas Vol 44, No 1 (2021)
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v44.i1.p1-16.2021

Abstract

Objective: To analyze the relationship between the intensity of nocturnal pruritus and the sleep quality of scabies patients. Method: This was an analytic study with cross-sectional design. The subject was recruited by total sampling. Data was analysed using the Spearman correlation test. Result: As many as 160 out of 302 students (53%) were suffering from scabies. Majority of patients were 13 years old, at the 7th grade of school, had a normal Body Mass Index (BMI), had an onset of scabies ≤ 3 months and not in the scabies treatment period in the last 1 month. There were 22 scabies patients matched the inclusion and exclusion criteria. As many as 5 out of 22 patients had mild nocturnal pruritus,10 patients had moderate nocturnal pruritus and 7 patients had severe nocturnal pruritus. There were 13.6% had good sleep quality and 86.4% had poor sleep quality. Analysis using Spearman test showed the value of p=0.65 implicated that there was no significant correlation between the intensity of nocturnal pruritus and the sleep quality of scabies patients. Conclusion: There was no relationship between the intensity of nocturnal pruritus and the sleep quality of scabies patients at the Pesantren male dormitory.
Development of standardized herbal therapy of bangle extract (Zingiber Cassumunar Roxb.) on the expression of Icam - 1 for complementary therapy to prevent complications in Malaria Wiwien Sugih Utami; Heni Fatmawati; Bagus Hermansyah
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 8, No 3, (2017)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol8.Iss3.art8

Abstract

Background : Malaria is still a major problem, both in international and national scale. Erythrocytes containing Plasmodium sp. will stimulate the release of Th1 (T helper-1) and release monocytes and macrophages to perform phagocytosis and produce tumor necrosis factor-alpha (TNF-α). The release of iNOS and free radicals would increase the expression of adhesion molecules such as ICAM-1, VCAM-1, CD36, and P-selectin. Research shows that IL- 10 can inhibit the production of TNF - α and IL - 1. TNF-α and ICAM - 1 play a role in the occurrence of cerebral malaria. Bangle (Zingiber cassumunar Roxb.) has a lot of potentials to be utilized as a complementary therapy to prevent the complications of malaria. Objective : This study aims to find the ability of Bangle (Zingiber cassumunar Roxb.) extract as an immunostimulant which would be used as a complementary therapy for standard malaria treatment. Methods : A number of 25 mice Balb/C males were divided into 5 groups, stimulated with methanol fractions of Bangle extract for 14 consecutive days before infected with Plasmodium berghei. All groups were examined for parasite daily, until day 7 post-positive malaria, referring to Artemisinindose regimen administered orally 0.04 mg/g bw/day for 7 days. Results : This study proves that the administration of Artemisinin combined with methanol fraction of Bangle extract provides better results to inhibit ICAM-1 gene expression in malaria-infected mice compared to single-therapy Artemisinin or methanol fraction of bangle extract. Conclusion : The combination of artemisinin and methanol fraction of Bangle extract can inhibit ICAM-1 gene expression in malaria-infected mice better compared to without the combination.
Hypertension as a Determining Factor in the Rupture of Intracranial Aneurysms, Diagnosed by 64-MDCT Angiography Fatmawati, Heni; Santoso, Antonius G
Makara Journal of Health Research Vol. 21, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: To determine a correlation between risk factors and the rupture of intracranial aneurysms. Methods: A cross-sectional study of 29 patients with a saccular intracranial aneurysm was obtained using consecutive sampling and examination of 64-MDCT angiography. Bivariate statistical analysis using Fisher's exact test was arranged using cross-tabulation to determine the correlation between each risk factor of age, sex, hypertension, and smoking with the occurrence of ruptured intracranial aneurysms. Results: The highest incidence of ruptured intracranial aneurysms were in patients aged < 60 years (70%), male (75%), experienced hypertension (85%), and were smokers (85.7%). Only the risk factor of hypertension had a correlation with the occurrence of a ruptured intracranial aneurysm (p < 0.05). The prevalence ratio of age and sex were 1.0 and 0.9, whereas hypertension and smoking were 2.6 and 1.3. Conclusions: The risk factor of hypertension leading to a ruptured intracranial aneurysm was 2.6 times higher than non-hypertensive patients, and as such hypertension is a risk factor associated with the occurrence of ruptured intracranial aneurysms
Gambaran faktor risiko dan tingkat risiko stroke iskemik berdasarkan stroke risk scorecard di RSUD Klungkung Ni Made Trismarani Sultradewi Kesuma; Dion Krismashogi Dharmawan; Heni Fatmawati
Intisari Sains Medis Vol. 10 No. 3 (2019): (Available online: 1 December 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.353 KB) | DOI: 10.15562/ism.v10i3.397

Abstract

Latar Belakang: Indonesia memiliki angka beban stroke terbanyak kedua setelah Mongolia yaitu sebanyak 3.382,2/100.000 orang berdasarkan DALYs (disability-adjusted life-year). Berdasarkan profil kesehatan provinsi Bali, tipe stroke yang memiliki tingkat prevalensi yang tinggi adalah stroke iskemik. Strategi praktis dalam mengatasi beban akibat stroke harus difokuskan pada pencegahan dan penanganan berdasarkan faktor risiko. Tujuan penelitian ini adalah untuk mengetahui hubungan antara faktor-faktor risiko dengan tingkat risiko penderita stroke iskemik yang rawat inap di RSUD Klungkung Bali periode Oktober 2017 sampai dengan September 2018.Metode: Penelitian ini merupakan penelitian analitik observasional dengan desain cross sectional. 65 Subyek penelitian yang sesuai dengan kriteria inklusi, diambil dari data rekam medis pasien stroke iskemik yang rawat inap di RSUD Klungkung. Variabel yang diteliti dalam penelitian ini yaitu usia, jenis kelamin, tekanan darah, kadar kolesterol, riwayat merokok, indeks massa tubuh (IMT), aktivitas fisik, riwayat diabetes, riwayat fibrilasi atrium, dan riwayat stroke dalam keluarga.Hasil: Dari total 65 pasien stroke iskemik yang rawat inap di RSUD Klungkung, paling banyak berada pada kelompok usia ≥ 55 tahun – 64 tahun dengan usia rerata 61,45 ± 12,016 tahun. Pasien berdasarkan jenis kelamin didominasi oleh laki-laki dengan perbandingan kejadian stroke iskemik antara laki-laki dan perempuan sebesar 2,4:1. Hasil penilaian tingkat faktor risiko dengan Stroke Risk Scorecard (SRS), 51 orang (78,5%) memiliki risiko tinggi, 9 orang (13,8%) memiliki risiko sedang, dan 5 orang (7,7%) memiliki risiko rendah.Simpulan: Uji statistik chi-square menunjukkan adanya hubungan yang signifikan antara tingkat risiko stroke iskemik dengan indeks massa tubuh, tekanan darah, riwayat merokok, riwayat diabetes, dan kadar kolesterol (p<0,005).Introduction: Indonesia is the second highest country of stroke burden after Mongolia, which are 3,382.2 / 100,000 people based on DALYs (disability-adjusted life-year). Based on the health profile of the province of Bali, the type of stroke that has high prevalence rate is ischemic stroke. Practical strategies in overcoming the burden of stroke must be focused on prevention and treatment based on risk factors. The study aims to determine the relationship between risk factors and the level of risk of ischemic stroke patients hospitalized at RSUD Klungkung Bali.Methods: This study is observational analytic study with a cross sectional design that includes 65 patients taken from the medical records. The variables studied are age, gender, pressure blood, cholesterol levels, smoking history, body mass index (BMI), physical activity, history of diabetes, history of atrial fibrillation, and history of stroke in the family. Data analysis techniques used were univariate analysis and bivariate analysis.Result: The most characteristics based on age group is ≥ 55 years - 64 years with an average age of 61.45 ± 12.016 years. Patients are dominated by men (2,4:1). The assessment of the level of risk factors in each sample using the Stroke Risk Scorecard (SRS), 51 people (78.5%) had a high risk, 9 people (13.8%) had moderate risk, and 5 people (7.7%) has a low risk of ischemic stroke.Conclusion: The chi-square statistical analysis showed a significant relationship between the risk level of ischemic stroke with body mass index, blood pressure, smoking history, history of diabetes, and cholesterol levels (p<0,005).
Kontaminasi Sumber Air oleh Cacing Usus dan Higiene Sanitasi sebagai Faktor Risiko Infeksi Helminthiasis pada Petani Yunita Armiyanti; Brillian Adexa Yudinda; Heni Fatmawati; Bagus Hermansyah; Wiwien Sugih Utami
Jurnal Kesehatan Lingkungan Indonesia Vol 22, No 1 (2023): Februari 2023
Publisher : Master Program of Environmental Health, Faculty of Public Health, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkli.22.1.60-68

Abstract

Latar belakang: Kabupaten Jember khususnya Kecamatan Jenggawah merupakan wilayah agraris dengan pekerjaan utama sebagai petani dan hampir separuh penduduknya tidak mempunyai fasilitas jamban untuk buang air besar. Kebiasaan defekasi yang tidak sehat masih banyak dilakukan oleh masyarakat petani, sehingga bisa mencemari sumber air dengan telur dan larva cacing usus yang berakibat terjadinya penularan helminthiasis. Penelitian ini bertujuan untuk mengetahui adanya kontaminasi sumber air (air sumur dan sungai) oleh cacing usus, faktor risiko higiene sanitasi dan hubungannya dengan prevalensi helminthiasis pada petani di Kecamatan Jenggawah, Kabupaten Jember.Metode: Penelitian ini dilakukan di Kecamatan Jenggawah yang sebagian besar masyarakatnya adalah petani. Jumlah petani yang bersedia terlibat dalam penelitian adalah 56, sehingga jumlah sampel feses dan air sumur yang diperoleh juga sebanyak 56. Sampel air sungai juga diambil untuk diperiksa. Sampel feses dan air diperiksa menggunakan metode sedimentasi dan flotasi untuk identifikasi telur dan larva cacing usus. Faktor-faktor risiko higiene dan sanitasi sebagai variabel bebas didapatkan dari kuesioner dan dianalisis dengan univariat, bivariat dan multivariat.Hasil: Karakteristik responden menunjukkan sebagian besar adalah laki-laki (53,51%), berusia 18-60 tahun (75%), berpendidikan rendah (53,57%), dan berpenghasilan rendah (71,43%). Sampel air sumur yang terkontaminasi telur dan larva cacing usus sebesar 22,2%, sedangkan sampel air sungai semuanya terkontaminasi. Prevalensi helminthiasis pada petani adalah 7,1%. Jenis cacing usus yang banyak ditemukan adalah Hookworm. Hasil uji bivariate (chi-square) menunjukkan kontaminasi sumber air oleh cacing usus berhubungan dengan prevalensi helminthiasis (ρ<0,05). Faktor risiko sanitasi yaitu jenis sumber air (air sungai atau air tanah) dan faktor risiko higiene kebiasaan mandi,cuci dan kakus (MCK) di sungai berhubungan dengan prevalensi helminthiasis (ρ<0,05).Simpulan: Sumber air yang terkontaminasi oleh telur dan larva cacing usus dan penggunaannya untuk kegiatan MCK dapat menjadi sumber transmisi infeksi helminthiasis pada masyarakat petani. ABSTRACTTitle: Contamination of Water Sources by Intestinal Worms and Sanitary Hygiene as Risk Factors for Helminths Infection in Farmers.Background: Jember Regency, especially Jenggawah Subdistrict, is an agricultural area with the main occupation as farmers and almost half of the population does not have latrine facilities for defecation. Unhealthy defecation habits are still widely practiced by farming communities, so that they can contaminate water sources with eggs and larvae of intestinal worms which result in helminthiasis transmission. This study aims to determine the contamination of water sources (well and river water) by intestinal worms, sanitation hygiene risk factors and their association with the prevalence of helminthiasis in farmers in Jenggawah District, Jember Regency.Method: This research was conducted in Jenggawah District where most of the people are farmers. The number of farmers who were willing to be involved in the study was 56, so the number of faecal and well water samples obtained was also 56. River water samples were also taken for examination. Stool and water samples were examined using sedimentation and flotation methods to identify eggs and larvae of intestinal worms. Hygiene and sanitation risk factors as independent variables were obtained from the questionnaire and analyzed using univariate, bivariate and multivariate analysis.Result: The characteristics of the respondents showed that most of them were male (53.51%), aged 18-60 years (75%), low educated (53.57%), and low income (71.43%). Well water samples were contaminated with eggs and larvae of intestinal worms by 22.2%, while river water samples were all contaminated. The prevalence of helminthiasis is 7.1%. The most common type of intestinal worm is Hookworm. The results of the chi-square test showed that contamination of water sources by intestinal worms was associated with the prevalence of helminthiasis (ρ<0.05). Sanitation risk factors, namely the kind of water sources (river water or ground water) and hygiene risk factors, i.e bathing, washing and latrine activities (MCK) habits in rivers are associated with the prevalence of helminthiasis (ρ<0.05).Conclusion: Water sources contaminated by eggs and larvae of intestinal worms and the habit of bathing, washing and latrine activities (MCK) in the river can be a source of transmission of helminthiasis infection in farming communities.
Correlation between Vertebral Slippage in Spondylolisthesis with Ligamentum Flavum Thickening in Dr. Soebandi Hospital, Jember, East Java, Indonesia Heni Fatmawati; Alvin Candra; Nindya Shinta Rumastika; Al Munawir; Muhamad Hasan; I Nyoman Semita
Althea Medical Journal Vol 10, No 1 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n1.2794

Abstract

Background: Spondylolisthesis is a vertebral slip condition that compromises the body’s axial alignment and structures such as ligamentum flavum. The ligamentum flavum thickening may play a role in spondylolisthesis. The purpose of this study was to investigate the correlation between the slippage of vertebral body (spondylolisthesis) with ligamentum flavum thickening as a tissue response regarding increased mechanical stress.Methods: An analytical retrospective radiological observational was used a cross-sectional study from the results of MRI examination in Dr. Soebandi Hospital, Jember, Indonesia, collected from 2018-2021 using the simple random sampling method. Ligamentum flavum thickness and the degree of vertebral slip were measured using T2-weighted MR imaging and the correlation was analyzed using Fisher’s exact test. Results: Of 102 spondylolisthesis data records, 34 were taken, being female was the most prevalent cases in the age group above 60 years, with the most vertebral segment shift occurring at the L4–L5 level. There was a significant correlation. (p = 0.02; RR = 4.8) between ligamentum flavum thickening and vertebral slip in spondylolisthesis. Conclusion: The significant correlation between ligamentum flavum thickening and vertebral slip in spondylolisthesis suggest that mechanical stress has caused spondylolisthesis, by inducing chronic inflammation that results in hypertrophy of ligamentum flavum. Lumbar segmental instability due to spondylolisthesis is a factor contributing to the development of ligamentum flavum hypertrophy.
Knowledge and Behavior as Risk Factors for Intestinal Worm Contamination on Raw Vegetables Food Traders in Pakusari District Jember Regency Yoga Setyo; Wiwien Sugih Utami; Heni Fatmawati; Yunita Armiyanti; Bagus Hermansyah
Jurnal Ilmiah Kesehatan Vol 16 No 01 (2023): Jurnal Ilmiah Kesehatan (Journal of Health Science) 
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/jhs.v16i01.3192

Abstract

Intestinal parasites are one of the leading public health problems worldwide, with an incidence of 24% worldwide and 45-65% in Indonesia. Intestinal parasites, especially intestinal worms, or soil-transmitted helminths (STH), significantly contribute to gastrointestinal diseases worldwide. Intestinal worm infections occur in rural and urban populations, especially in people who have the habit of eating with dirty hands, using unhygienic toilets, and consuming contaminated food, water, or soil. Fresh vegetables are a means that support parasitic intestinal infections, especially for traders who must be able to prevent intestinal worm contamination in fresh vegetables. Consumption of raw vegetables can be a source of intestinal worm infection, thereby increasing the prevalence of foodborne diseases. This type of research is analytic observational with a cross-sectional study design. Samples in the form of fresh vegetables were taken from fresh vegetable traders in Pakusari District, Jember Regency and then processed by the sedimentation method and then observed using a microscope to identify intestinal protozoa. Food safety knowledge was assessed using a questionnaire, and the behavior of food traders was assessed by researchers using observation sheets. The bivariate analysis showed that proper handwashing behavior was related to intestinal worms in fresh vegetables (p<0,05). Other knowledge and behavior variables were unrelated to intestinal worms' presence. The intestinal worm species found is a type of Hookworm.
Clinical Efficacy and Safety of Microwave Ablation Compared to Radiofrequency Ablation in Hepatocellular Carcinoma Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials Rifaldy Nabiel Erisadana; Yehuda Tri Nugroho Supranoto; Heni Fatmawati; Irawan Fajar Kusuma; Adrian Wibisono; Putu Ayu Laksmi Lestari
Indonesian Journal of Cancer Vol 17, No 3 (2023): September
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v17i3.988

Abstract

Background: Ablation modalities for the treatment of hepatocellular carcinoma (HCC) including microwave ablation (MWA) and radiofrequency ablation (RFA) are clinically important due to their numerous advantages. Several trials showed inconsistent results regarding safety and efficacy, making the comparison between MWA and RFA challenging. Therefore, this study aimed to enhance the evidence on treatment modalities regarding the clinical efficacy and safety of MWA compared to RFA in HCC patients. Methods: A systematic review and meta-analysis was conducted following the PRISMA guidelines. Subsequently, a literature search was carried out by PubMed, ScienceDirect, and Google Scholar for randomized controlled trials (RCTs) in HCC patients who passed through MWA compared to RFA. Quantitative analysis of pooled risk ratio with a 95% confidence interval was performed using Review Manager 5.4 software in a random-effects model or fixed-effects model forest plot.Results: Based on 9 RCTs included in the analysis, there were insignificant different results in terms of complete ablation rates (CA) [RR=1.01, 95%CI (0.99 to 1.03), p=0.47] and adverse events (AE) [RR=1.15, 95%CI (0.88 to 1.50), p=0.31]. However, lower incidence of local tumor progression (LTP) [RR=0.73, 95%CI (0.54 to 0.99), p=0.04], intrahepatic de novo lesions (IDL) [RR=0.90, 95%CI (0.81 to 1.00), p=0.05], and extrahepatic metastases (EHM) [RR=0.65, 95%CI (0.44 to 0.95), p=0.03] exhibited significant differences in MWA group.Conclusions: This meta-analysis provided evidence that MWA and RFA had equivalent CA rates and AE in HCC patients. However, MWA was considered superior to RFA due to a lower incidence of LTP, IDL, and EHM.
The impact of Pesticides and Workload on Osteoarthritis Incidence in Farmers: A Narrative Review Lailatul Maghfiroh; Heni Fatmawati; Ika Rahmawati Sutejo
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 11, No 1 (2022): MARET 2022
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jikw.v11i1.1441

Abstract

An osteoarthritis is a group of pathologies in joints that include cartilage degradation, bone remodeling, osteophyte formation, and synovial inflammation which in turn can cause pain, stiffness, swelling, and loss of normal joint function. The increasing prevalence of osteoarthritis is associated with jobs that require physical strength (physically demanding) or heavy work, one of which is agriculture. This is in line with the World Health Organization's statement, farming for 1-9 years increases the risk of osteoarthritis 4.5 times. Besides, farming for 10 years or more increases the risk of osteoarthritis by 9.3 times. The purpose of this study was to determine the association between pesticide use and workload (which includes work attitude, working period, and heavylifting) and the incidence of osteoarthritis in farmers. This study uses a narrative review method on journals of the last 10 years. The results of this study indicate that pesticides are not associated with osteoarthritis, based on studies on osteoarthritis patients that show no difference in serum concentrations of organophosphate, pyrethroid, and nenonicotinoid pesticides and their metabolites. Conversely, work attitude and working period are associated with osteoarthritis in farmers. The work attitude associated with osteoarthritis especially knee osteoarthritis is the squatting and kneeling posture. Meanwhile, the heavylifting did not have a significant relationship with osteoarthritis, especially knee osteoarthritis among farmers. The conclusion of this study is there is no relationship between the use of pesticide and osteoarthritis. However, there is a relationship between work attitudes & working period and osteoarthritis in farmers.