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All Journal Jurnal Biologi Tropis Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdi Insani BIOEDUPAT: Pattimura Journal of Biology and Learning Pakem : Jurnal Pengabdian Kepada Masyarakat Rumphius Pattimura Biological Journal
Mery Pattipeilohy, Mery
FKIP Universitas Pattimura Ambon
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Public Health Social Sciences Other

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APPLICATION OF EXPERIMENTAL METHODS AND ASSIGNMENT TO IMPROVE SCIENTIFIC WORK ON COMMUNICATED THE MORPHOLOGICAL CHARACTERISTICS OF GANDARIA (Bouea macrophilla) IN JUNIOR HIGH SCHOOL STUDENTS Warbanaran, Manselin; Pattipeilohy, Mery; Papilaya, Pamella Mercy
RUMPHIUS Vol 4 No 1 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i1p001-003

Abstract

This study aims to determine the improvement of skills in communicated plant morphological characteristics in Junior High School students in Ambon used the application of the experimental method and the method of gived assignments. Find out the differences in the skills of communicating plant morphological characteristics in Junior High School students used the application of the experimental method and used the assignment method. The research used in this study is a qualitative and quantitative descriptive. The subjects of the study were 7th grade students. The samples taked in this study were divided into 12 classes, namely the experimental class, 4 classes, the assignment class, 4 classes and the control class, 4 classes each received different treatment. Data analysis was carried out experimentally. Results were calculated manually and Anacova statistical analysis. The result is a different test or comparative test with the dependent variable having an interval or ratio data scale, while the independent variables consist of a mixture of categorical data and numerical data.
ANALISIS KEBERADAAN BAKTERI ENTEROBACTER PADA AIR CUCIAN IKAN LAYANG (DECAPTERUS SPP) DI PASAR MARDIKA DAN PASAR TAGALAYA AMBON Manusiwa, Gellian Gabriella; Pattipeilohy, Mery; Pelamonia, Alamanda; Mahulette, Ferymon
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page42-51

Abstract

Background: Fish sales generally do not prioritize sanitation aspects in Ambon city. This study aims to determine the presence of Enterobacter bacteria and the morphological characteristics of Enterobacter bacteria in the washing water of flying fish (Decapterus spp) at Mardika Market and Tagalaya Market Ambon. Wash water samples were taken at Mardika and Tagalaya Markets in Ambon. Methods: The analysis used the Most Probable Number (MPN) method and biochemical tests (methyl red and citrate tests). Results: The results showed that 6 samples of flying fish washing water at the mardika market and tagalaya market that had been tested were identified as Enterobacter bacteria and based on the morphological characteristics found in the washing water samples of flying fish were pink, smooth edges, wavy, curved, and also not sequential and the elevation was flat and raised. The methyl red and citrate tests were negatife and positive respectively. Conclusion: Flying fish washing water at the Mardika and Tagalaya Markets has been contaminated with Enterobacter bacteria.
THE EFFECT OF PROBLEM BASED LEARNING AND INQUIRY LEARNING LEARNING MODELS ON ECOSYSTEM CONCEP TO IMPROVE CRITICAL THINKING, AND CREATIVE THINKING, ABILITY OF STUDENTS SMA NEGERI 11 AMBON, SMA NEGERI 13 AMBON AND MADRASAH ALIYAH NEGERI AMBON. Serang, Nur Fatahila; Pattipeilohy, Mery; Rehena, Johanis Fritzgal
RUMPHIUS Vol 3 No 2 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i2p043-046

Abstract

Many abilities that students must have, namely the ability to think critically, think creatively, construct knowledge, solve problems and master learning material well. One of the efforts to improve students' critical thinking and creative thinking skills is to change the old learning model used with the problem-based learning model and inquiry learning model. Students think more critically and think creatively and encourage students to be able to connect between the knowledge they have and its application in their daily lives. This study aims to determine the effect of two learning models, namely problem-based learning and inquiry learning to improve critical thinking skills and creative thinking on ecosystem material in class X SMAN 11, SMAN 13, and Madrasah Aliyah. The type of research is Quasi Experiment using 3 experimental classes using the problem based learning model and 3 experimental classes using the inquiry learning model. The statistical analysis used was multiple linear regression using SPSS 22. The results obtained from multiple linear regression analysis both had significant values <0.05 using both problem based learning and inquiry learning models at SMA N 11, SMA N 13 and Madrasah Aliyah Negeri Ambon. Conclusion. There is an influence of the use of problem-based learning models and inquiry learning models to improve the ability to think in class X SMAN 11, SMAN 13 and Ambon Madrasah Aliyah.
EFFECTIVENESS OF ANTISEPTIC BASED ON GANDARIA LEAVES (Bouea macrophylla Griff) ON THE GROWTH OF Staphylococcus aureus AND Escherichia coli Lelapary, Claudia; Pattipeilohy, Mery; Mahulette, Ferymon
RUMPHIUS Vol 4 No 2 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i2p047-051

Abstract

Gandaria leaves (Boea macrophylla Griff) have potential as antibacterial. The ability to inhibit bacterial growth is inseparable from the role of active compounds, namely flavonoids, tannins, quinones and triterpenoids. Growth by microbes needs to be controlled, namely by inhibiting microorganisms, one way is by using chemicals, namely antiseptics. This study aims to determine the effectiveness of antiseptics made from gandaria leaves against the growth of S. aureus and E. coli bacteria. The concentrations of the ethanol extract of gandaria leaves used were 5%, 15% and 25% and betadine (+) and aquades (-) were used as controls. Antiseptic effectiveness test against S. aureus and E. coli bacteria using disc diffusion method. The test results showed that the antiseptic based on gandaria leaves had the ability to inhibit the growth of S. aureus with a concentration of 15% at 2.00 mm and 25% at 4.00 mm. Meanwhile, for E. coli bacteria, antiseptic made from gandaria leaves has an inhibition zone at a concentration of 15% at 1.00 mm. In accordance with the criteria for antiseptic power according to Davis Stout, gandaria leaves which are used as basic ingredients for antiseptics are included in the criteria for very weak antiseptic power (≤5). Therefore, the antiseptic based on gandaria leaves in this study was less effective in inhibiting the growth of S. aureus and E. coli bacteria.
PENGARUH EKSTRAK KULIT MANGGIS (Garcinia Mangostana L) TERHADAP KADAR FLAVONOID DAN KUALITAS ORGANOLEPTIK NATA DE SOYA Kalkoy, Yosualda; Pattipeilohy, Mery; Sangur, Kristin; Mahulette, Ferymon
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page237-242

Abstract

Background: Mangosteen (Garcinia mangostana L) is one of Indonesia's tropical fruits. Mangosteen peel extract can be used as a natural colorant for a variety of products such as medicines, cosmetics, beverages and processed foods. Mangosteen peel contains compounds of flavonoids, saponins, tannins, steroids/triterpenoids. Anthocyanins in mangosteen skin can be used as a natural colorant for processed food products nata de soya made from tofu liquid waste. Research Methods: This research was conducted on April 17 - August 21, 2023 at the Basic Chemistry Laboratory of FMIPA and Biology Laboratory of FETT. This type of research is quantitative experimental research, arranged in a completely randomized design. With four treatments of flavonoid levels of 10, 20, 30, 40% mangosteen peel extract and one control. Results: The results showed that there was an effect of mangosteen peel extract as a natural colorant on the flavonoid content of nata de soya. Conclusion: The Smallest Real Difference Test (BNT) at the significance level α 0.05 and the significance level α 0.01 and the results of the BNT Test at the 0.05% level and the 0.01% level for each indicator show that the provision of mangosteen peel extract to the flavonoid content of nata de soya, gives very different results.
PRAKTIKUM PEMBUATAN MEDIA PERTUMBUHAN MIKROBA SEMI SINTESIS DAN STERILISASI MENGGUNAKAN ALAT SEDERHANA PADA SISWA SMA NEGERI 28 MALUKU TENGAH Pelamonia, Alamanda; Pattipeilohy, Mery; Mahulette, Ferymon; Sahetapy, Elsa Josma
Jurnal Abdi Insani Vol 12 No 5 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i5.2495

Abstract

Pelaksanaan praktikum di sekolah sering menghadapi kendala, seperti keterbatasan sarana dan prasarana, kurangnya kreativitas dalam perancangan topik, serta minimnya pemanfaatan bahan alami sebagai alternatif dalam praktikum Biologi. SMA Negeri 28 Maluku Tengah mengalami tantangan serupa, terutama dalam praktikum mikrobiologi yang memerlukan alat khusus, kondisi steril, serta biaya media pertumbuhan mikroba yang tinggi. Selain itu, waktu inkubasi yang lama (2–3 hari) semakin membatasi implementasi praktikum ini, sehingga guru kesulitan mengajarkan konsep mikrobiologi secara langsung kepada siswa. Pelaksanaan pengabdian ini bertujuan untuk meningkatkan keterampilan kerja siswa melalui pendampingan dan pelatihan dalam pembuatan media pertumbuhan mikroba semi-sintesis serta sterilisasi menggunakan alat sederhana. PKM dilaksanakan melalui tahapan persiapan, koordinasi dengan sekolah, penyusunan materi, dan penggunaan alat sederhana. Pelaksanaan menggunakan metode ceramah, tanya jawab, dan eksperimen kelompok. Evaluasi dilakukan melalui kuisioner dan umpan balik untuk menilai pemahaman dan kepuasan siswa. Pelatihan ini berhasil meningkatkan keterampilan praktis siswa, memungkinkan mereka untuk memahami dan menerapkan metode pembuatan media serta teknik sterilisasi dengan bahan yang lebih mudah diakses tanpa mengurangi kualitas eksperimen. Selain itu, kegiatan ini berkontribusi pada peningkatan pemahaman siswa terhadap konsep mikrobiologi, penguatan keterampilan berpikir kritis dan pemecahan masalah, serta tumbuhnya minat mereka terhadap sains. Inovasi ini memungkinkan mereka untuk melaksanakan praktikum mikrobiologi dengan alat dan bahan yang lebih terjangkau, tanpa mengurangi kualitas eksperimen yang dilakukan.
Characteristics and Potential of Nitrogen-fixing Microbes in the Rhizosphere of Corn and Legume Plants on Kisar Island Mahulette, Ferymon; Pattipeilohy, Mery; Pelamonia, Alamanda
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9646

Abstract

The Kisar Island community usually plants legumes and corn in one hole called the hapa planting system. This planting system allows interaction between microbes, especially in the rhizosphere that supports plant growth. This study aims to characterize and test the potential of nitrogen-fixing microbes in the rhizosphere of legumes and corn plants using the hapa planting system. Isolation and characterization of nitrogen-fixing microbes using Yeast extract mannitol agar (YEMA) media added with Congo red and bromothymol blue as indicators. The potential of the microbes tested was the ability to dissolve phosphate using Picovskaya media and cellulolytic using Carboxy methyl cellulose (CMC) media. A total of five isolates of nitrogen-fixing microbes were found each in the rhizosphere of corn and mung beans (JKH) and corn and red beans (JKM) with varying numbers and characteristics. The number of microbes in the JKH rhizosphere was 9.5x102 cfu/g lower than the number of microbes in JKM which was 1.5x103 cfu/g. The highest phosphate-dissolving ability was found in isolate KM5 with a phosphate solubility index of 3.14, while the cellulolytic ability was found in isolate KH1 with a cellulolytic index of 0.80. These potential microbes can be developed as starters for developing biofertilizers on less fertile agricultural land in the future.
THE EFFECT OF LONG BOIL BLACK CASSUM (Manihot aipiphol) ON THE PRESENCE OF BACTERIA Masbait, Meske; Rehena, Johanis Fritzgal; Pattipeilohy, Mery
RUMPHIUS Vol 3 No 1 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i1p011-014

Abstract

Processing of black cassava has the possibility of microbial contamination because the fermentation process is carried out in the open, black cassava in the open, at room temperature, allowing microbes such as bacteria to grow. The aim of this research is to determine the length of time for boiling cassava and the presence of bacteria, and to determine the appropriate boiling time that can inhibit the presence of bacteria. From the results of observations of cassava samples containing bacteria before boiling, this shows that fresh cassava used as raw material naturally contains a number of bacteria. Observing the colony characteristics of all bacterial isolates counted and uncounted, it was found that there were similarities and differences in properties, where the results of the analysis showed that the long boiling time treatment had a great influence on the morphology of the bacteria, namely round and wrinkled, with smooth and grooved edges, raised elevations, flat and thick. The color of the bacteria on black cassava is the same, namely white, with treatment times of 10, 20, 30 minutes. The boiling time treatment has a very significant effect on the shape, edges and elevation in the presence of bacteria. The presence of bacteria in black cassava before boiling shows that it naturally contains a number of bacteria, with long boiling treatment the number of bacteria increases.
Feasibility of an Instrument for Assessment of High Order Thinking Skills (HOTS) Material on Environmental Change for High School Students in Ambon City Tuhumury, Benezia; Awan, Ali; Pattipeilohy, Mery
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 5 No 2 (2025): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v5.i2.pp327-334

Abstract

High Order Thinking Skills (HOTS) is interpreted as the ability to think in complex processes in decomposing material, criticizing and creating solutions to problems. The low percentage of Higher Order Thinking Skills questions in the pre-observation results is an indicator of low cognition of students in school. This study aims to determine the feasibility and effectiveness of the assessment instrument for high order thinking skills (HOTS) environmental change material in class X of high school in Ambon City. This type of research is development (R&D) with a quantitative approach designed to obtain products in the form of HOTS question measuring tools. The research population is State Senior High School 3 Ambon, State Senior High School 5 Ambon and State Senior High School 11 Ambon with a sample of 103 students. The research instruments used are HOTS test question instruments and test instrument validation sheets. The data analysis technique uses a feasibility analysis of test instruments based on HOTS questions. The results of the study show that :The HOTS question instrument for environmental change material is very feasible to be used as an assessment instrument in high schools in Ambon City. This means that the HOTS question instrument is very worthy of being given to students. HOTS-based assessment instruments on environmental change materials are effectively used as assessment instruments in high schools in Ambon City, this can be seen from the ability of students to work on question item instruments that are assessed individually. The students' scores obtained from the results of working on the test items were then averaged to obtain one score as the result of the students' HOTS thinking abilities, namely 1.27 for State Senior High School 3 Ambon, 1.71 for State Senior High School 5 Ambon, and 1.82 for State Senior High School 11 Ambon.
Analysis of coliform bacteria contamination in drinking water refill in Ambon City Nikijuluw, Monica; Pattipeilohy, Mery; Mahulette, Ferymon
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 3 No 2 (2023): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v3.i2.pp125-128

Abstract

In recent years, refillable drinking water businesses have grown rapidly in several cities in Indonesia, including Ambon City. On the one hand, Refillable Drinking Water Depots (DAMIU) support efforts to realize a healthy society by expanding the reach of clean water consumption, On the other hand, DAMIU tend to be problematic when faced with business interests. Feces contain coliform bacteria which is an indicator of water contamination. This study aims to determine the presence of coliform bacteria in Ambon City. The samples used in the study came from three sub-districts of refill drinking water depot sources, namely the Teluk Dalam sub-district, Nusaniwe sub-district, and Baguala sub-district. Microbiological testing of refill drinking water using MPN (most probable number) method. The MPN test results showed that three refill drinking water samples were not contaminated with coliform bacteria because there was no turbidity or gas formation in the Durham tube for gas-forming bacteria. This indicates that the three refill drinking water samples tested microbiologically have good quality and are safe for consumption, because in 100 ml of water no coliform bacteria were found in accordance with Permenkes No. 492/Menkes/Per/IX/2010 regulations on the requirements and supervision of water quality, which states that the microbiological requirements for drinking water are MPN Coliform /100 ml sample = 0. There are no coliform bacteria in the refillable drinking water depot in Ambon City so the water is suitable for consumption
Co-Authors Awan, Ali Ferymon Mahulette, Ferymon Kalkoy, Yosualda Lelapary, Claudia Manusiwa, Gellian Gabriella Masbait, Meske Nikijuluw, Monica Papilaya, Pamella Mercy Pelamonia, Alamanda Rehena, Johanis Fritzgal Sahetapy, Elsa Josma Sangur, Kristin Serang, Nur Fatahila Tuhumury, Benezia Warbanaran, Manselin