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All Journal Jurnal Biologi Tropis Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdi Insani Pakem : Jurnal Pengabdian Kepada Masyarakat Jurnal MAIYAH Berita Biologi
Pelamonia, Alamanda
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ANALISIS KEBERADAAN BAKTERI ENTEROBACTER PADA AIR CUCIAN IKAN LAYANG (DECAPTERUS SPP) DI PASAR MARDIKA DAN PASAR TAGALAYA AMBON Manusiwa, Gellian Gabriella; Pattipeilohy, Mery; Pelamonia, Alamanda; Mahulette, Ferymon
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page42-51

Abstract

Background: Fish sales generally do not prioritize sanitation aspects in Ambon city. This study aims to determine the presence of Enterobacter bacteria and the morphological characteristics of Enterobacter bacteria in the washing water of flying fish (Decapterus spp) at Mardika Market and Tagalaya Market Ambon. Wash water samples were taken at Mardika and Tagalaya Markets in Ambon. Methods: The analysis used the Most Probable Number (MPN) method and biochemical tests (methyl red and citrate tests). Results: The results showed that 6 samples of flying fish washing water at the mardika market and tagalaya market that had been tested were identified as Enterobacter bacteria and based on the morphological characteristics found in the washing water samples of flying fish were pink, smooth edges, wavy, curved, and also not sequential and the elevation was flat and raised. The methyl red and citrate tests were negatife and positive respectively. Conclusion: Flying fish washing water at the Mardika and Tagalaya Markets has been contaminated with Enterobacter bacteria.
Penilaian Ekonomi Ekosistem Mangrove di Desa Tritih Kulon dan Karangtalun, Kabupaten Cilacap Chairummah, Annis; Wisudyanti, Dewi; Riviani, Riviani; Ekasanti, Anandita; Listiowati, Emyliana; Nugrayani, Dewi; Pelamonia, Alamanda
MAIYAH Vol 3 No 3 (2024): Maiyah : Vol. 3 No. 3 September 2024
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2024.3.3.13144

Abstract

Mangrove forests function as a place for the survival of various marine biota such as fish, shrimp, and crabs, especially the mangrove forest area in Tritih Kulon and Karangtalun Villages, Cilacap Regency. Mangrove forests in the area also function as a place for the reproduction of living things that can support the economy of the local community. The purpose of this internship is to determine the economic potential of the mangrove ecosystem and to determine the economic impact of the existence of the mangrove ecosystem on the income of local communities in Tritih Kulon and Karangtalun Villages, Cilacap Regency. This internship was carried out by conducting interviews and distributing questionnaires to obtain data. The results of the internship showed that the economic potential of mangrove forests in the Tritih Kulon area is quite high, namely IDR 330,430,000 and IDR 53,700,000 in Karangtalun Village. The existence of mangrove forests in both locations brings economic value to the local community through tourism activities, fisheries, and home industry production. It is hoped that this research can be a reference in the development of mangrove ecosystems in the future.
KADAR KLOROFIL ALGA HIJAU Dictyosphaeria versluysii DAN UJI SENSORI PRODUK OLAHANNYA Kurnia, Tri Santi; Ferdianti, Vicky; Pelamonia, Alamanda; Liline, Sintje
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 2 (2025): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue2page168-176

Abstract

Background: Dictyosphaeria versluysii is a type of green algae that can be consumed. This alga is abundant in the coastal waters of Maluku Province. However, this alga has not been widely explored scientifically. Generally, people who live on the coast of Ambon Island and its surroundings do not know about the potential of Dictyospaheria versluysiii algae. This study aims to determine the chlorophyll content of green algae Dictyospaheria versluysiii and the level of public preference for processed products. Methods: Samples of green algae Dictyospaheria versluysii were taken by purposive sampling. Analysis of chlorophyll content using UV-Vis Spectrophotometry method at wavelengths of 645 nm and 663 nm. Sensory test using questionnaire with Likert scale on 35 panelists in Tulehu District, Central Maluku Regency. Results: Chlorophyll levels in three different samples, namely the untreated wet sample (Dv.2), the untreated dry sample (Dv.1), and the treated wet sample (Dv.3), have different results. The total chlorophyll levels for the three samples were 35.1 mg/L, 59.7 mg/L, and 18.6 mg/L, respectively. Sample Dv.1 had higher chlorophyll levels compared to the other two samples. The results of the sensory test showed that the parameters of color, taste, aroma, and level of liking were in the “Very Like” criteria and texture was in the “Like” criteria. Conclusion: The chlorophyll content of Dictyospaheria versluysiii algae decreased after processing. However, processed Dictyosphaeria versluysii algae products can be accepted by the people of Negeri Tulehu. This algae can be developed into useful alternative and innovative food.
PRAKTIKUM PEMBUATAN MEDIA PERTUMBUHAN MIKROBA SEMI SINTESIS DAN STERILISASI MENGGUNAKAN ALAT SEDERHANA PADA SISWA SMA NEGERI 28 MALUKU TENGAH Pelamonia, Alamanda; Pattipeilohy, Mery; Mahulette, Ferymon; Sahetapy, Elsa Josma
Jurnal Abdi Insani Vol 12 No 5 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i5.2495

Abstract

Pelaksanaan praktikum di sekolah sering menghadapi kendala, seperti keterbatasan sarana dan prasarana, kurangnya kreativitas dalam perancangan topik, serta minimnya pemanfaatan bahan alami sebagai alternatif dalam praktikum Biologi. SMA Negeri 28 Maluku Tengah mengalami tantangan serupa, terutama dalam praktikum mikrobiologi yang memerlukan alat khusus, kondisi steril, serta biaya media pertumbuhan mikroba yang tinggi. Selain itu, waktu inkubasi yang lama (2–3 hari) semakin membatasi implementasi praktikum ini, sehingga guru kesulitan mengajarkan konsep mikrobiologi secara langsung kepada siswa. Pelaksanaan pengabdian ini bertujuan untuk meningkatkan keterampilan kerja siswa melalui pendampingan dan pelatihan dalam pembuatan media pertumbuhan mikroba semi-sintesis serta sterilisasi menggunakan alat sederhana. PKM dilaksanakan melalui tahapan persiapan, koordinasi dengan sekolah, penyusunan materi, dan penggunaan alat sederhana. Pelaksanaan menggunakan metode ceramah, tanya jawab, dan eksperimen kelompok. Evaluasi dilakukan melalui kuisioner dan umpan balik untuk menilai pemahaman dan kepuasan siswa. Pelatihan ini berhasil meningkatkan keterampilan praktis siswa, memungkinkan mereka untuk memahami dan menerapkan metode pembuatan media serta teknik sterilisasi dengan bahan yang lebih mudah diakses tanpa mengurangi kualitas eksperimen. Selain itu, kegiatan ini berkontribusi pada peningkatan pemahaman siswa terhadap konsep mikrobiologi, penguatan keterampilan berpikir kritis dan pemecahan masalah, serta tumbuhnya minat mereka terhadap sains. Inovasi ini memungkinkan mereka untuk melaksanakan praktikum mikrobiologi dengan alat dan bahan yang lebih terjangkau, tanpa mengurangi kualitas eksperimen yang dilakukan.
Kajian Pertumbuhan dan Faktor Kondisi Ikan Gelodok (Mudskipper) dalam Hubungan dengan Kerapatan Mangrove di Kawasan Ekosistem Esensial Muara Kali Ijo Armaity, Nut Spenta; Wisudyanti, Dewi; Hendrayana, Hendrayana; Pelamonia, Alamanda
MAIYAH Vol 4 No 1 (2025): Maiyah : Vol. 4 No. 1 Maret 2025
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2025.4.1.15745

Abstract

Ekosistem mangrove merupakan salah satu ekosistem pesisir yang memiliki produktivitas tinggi dan berperan penting sebagai penyedia makanan bagi berbagai organisme, termasuk ikan glodok (mudskipper). Ikan glodok hidup di area muara hingga pesisir dan sangat bergantung pada hutan mangrove sebagai habitat mencari makan. Penelitian ini bertujuan untuk mengetahui tingkat kerapatan mangrove, bioekologi ikan glodok yang meliputi pola pertumbuhan dan faktor kondisi, serta hubungan antara kerapatan mangrove dengan kelimpahan ikan glodok di Kawasan Ekosistem Esensial (KEE) Muara Kali Ijo, Kabupaten Kebumen. Metode yang digunakan adalah purposive sampling, dengan analisis data menggunakan uji korelasi dan regresi. Hasil menunjukkan bahwa kerapatan mangrove tergolong sangat padat, berkisar antara 1800–3800 pohon per hektar. Pola pertumbuhan ikan glodok bersifat allometrik negatif dengan nilai b antara 2,33–2,8. Faktor kondisi ikan berkisar antara 1,00–1,02, yang menunjukkan kondisi tubuh ikan cenderung kurus. Uji regresi menghasilkan nilai r sebesar 0,75591, menunjukkan hubungan yang kuat antara kerapatan mangrove dan kelimpahan ikan glodok. Namun, hubungan tersebut bersifat negatif, yang berarti semakin tinggi kerapatan mangrove, kelimpahan ikan glodok justru menurun.
Characteristics and Potential of Nitrogen-fixing Microbes in the Rhizosphere of Corn and Legume Plants on Kisar Island Mahulette, Ferymon; Pattipeilohy, Mery; Pelamonia, Alamanda
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9646

Abstract

The Kisar Island community usually plants legumes and corn in one hole called the hapa planting system. This planting system allows interaction between microbes, especially in the rhizosphere that supports plant growth. This study aims to characterize and test the potential of nitrogen-fixing microbes in the rhizosphere of legumes and corn plants using the hapa planting system. Isolation and characterization of nitrogen-fixing microbes using Yeast extract mannitol agar (YEMA) media added with Congo red and bromothymol blue as indicators. The potential of the microbes tested was the ability to dissolve phosphate using Picovskaya media and cellulolytic using Carboxy methyl cellulose (CMC) media. A total of five isolates of nitrogen-fixing microbes were found each in the rhizosphere of corn and mung beans (JKH) and corn and red beans (JKM) with varying numbers and characteristics. The number of microbes in the JKH rhizosphere was 9.5x102 cfu/g lower than the number of microbes in JKM which was 1.5x103 cfu/g. The highest phosphate-dissolving ability was found in isolate KM5 with a phosphate solubility index of 3.14, while the cellulolytic ability was found in isolate KH1 with a cellulolytic index of 0.80. These potential microbes can be developed as starters for developing biofertilizers on less fertile agricultural land in the future.
PELATIHAN PEMBUATAN MEDIA PETUMBUHAN MIKROBA UNTUK MENINGKATKAN WORK SKILL GURU, RESEARCH SKILL SISWA JURUSAN IPA Pattipeilohy, Mery; Mahulette, Ferymon; Pelamonia, Alamanda
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.4.1.127-133

Abstract

Permasalahan yang sering dijumpai di sekolah-sekolah terkait pelaksanaan praktikum adalah minimnya peralatan karena kurangnya sarana dan prasana penunjang kegiatan praktikum, kurangnya kreativitas dalam menyusun topik praktikum dan minimnya pengatahuan guru dalam memanfaatkan bahan-bahan alami yang dapat dijadikan sebagai bahan alternatif dalam pelaksanaan proses praktikum. Permasalahan inilah yang dialami oleh guru biologi pada SMA Negeri 1 Seram Bagian Barat, dimana khususnya untuk konsep mikrobiologi tidak di lakukan praktikum, dikarenakan dalam pelaksanaannya membutuhkan alat dan bahan yang banyak juga proses kerja yang harus steril. Tetapi karena alat dan bahan yang tidak lengkap dan bahkan tidak tersedia karena bahan untuk pertumbuhan mikroba cukup mahal dan butuh waktu yang lama dalam proses inkubasi serta pengamatan mikroba (bakteri dan kapang) yakni memerlukan waktu 2-3 hari. Hal ini yang menyebabkan guru mengalami kesulitan untuk melakukan praktikum terkait konsep mikrobiologi sehingga prakitkum belum pernah dilakukan. Oleh karena itu, tujuan dari pelaksanaan pengabdian ini adalah untuk meningkatkan work skill guru dan research skill siswa pada sekolah tersebut melalui pendampingan dan pelatihan menggunakan draft penuntun praktikum. Berdasarkan hasil yang diperoleh diketahui bahwa kemampuan kerja dan keterampilan guru dan juga kemampuan siswa semakin meningkat.
KELIMPAHAN DAN KARAKTERISTIK BAKTERI DALAM BAKASANG BIA GARU (Tridacna gigas L) [Abundance and Characteristics of Bacteria in Bakasang Bia Garu (Tridacna gigas)] Mahulette, Ferymon; Pattipeilohy, Mery; Pelamonia, Alamanda
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.697

Abstract

Bakasang bia garu is a clam-based fermented product processed by the people of the Babar Islands, Maluku. Uncontrolled processing allows the presence of unwanted bacteria in these local food products. Preservatives added in the processing of bakasang bia garu are very diverse. This study aims to isolate and characterize lactic acid, halotolerant, and coliform bacteria from bakasang bia garu which are processed using different preservatives. Lactic acid bacteria were isolated using de Man Rogosa and Sharpe Agar (MRSA) media while halotolerant and coliform bacteria used Mannitol Salt Agar (MSA) and Eosin Methylene Blue Agar (EMBA) media, respectively. Characterization was carried out macroscopically and microscopically. The results showed that lactic acid bacteria were only found in bakasang with synthetic salt and vinegar preservatives of 3.5x103 CFU/g which was dominated by Pediococcus pentosaceous. Halotolerant and coliform bacteria have diverse characteristics that belong to the genera Staphylococcus, Escherichia, and Enterobacter. Bakasang bia garu needs to optimize its controlled processing to improve the quality of this local food product in the future
Co-Authors Armaity, Nut Spenta Chairummah, Annis Ekasanti, Anandita Emyliana Listiowati, Emyliana Ferdianti, Vicky Ferymon Mahulette, Ferymon Hendrayana Hendrayana, Hendrayana Kurnia, Tri Santi Liline, Sintje Manusiwa, Gellian Gabriella Mery Pattipeilohy, Mery Nugrayani, Dewi Riviani Riviani, Riviani Sahetapy, Elsa Josma Wisudyanti, Dewi