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Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium Adi Tiya Yanuar; Abd. Aziz Amin; Lutfi Ni'matus Salamah; Frentina Murty Sujadi; Ekwan Nofa Wiratno; Citra Satrya Utama Dewi; Andi Kurniawan
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.45528

Abstract

Abstract Hydrocarbon contamination is one of the most significant environmental problems, including in the marine environment. Several methods, such as chemical and physical remediation, have been constructed to remediate pollutants but remain side effects and have a relatively high cost. Bioremediation is an alternative method for degrading contaminants due to its eco-friendliness and low cost. During bioremediation, the enzyme oxygenase is produced by bacteria to degrade pollutants. However, the oxygenation enzyme's activity and compound profile during this bioremediation process are still sparsely explored. This study aimed to analyze oxygenation enzyme activity and compound profiles during the hydrocarbon bioremediation process by bacterial consortia of Pseudomonas aeruginosa and Rhodococcus erythopolis. The materials of this research were the oxygenase enzyme activity, compound profile, and amount of BOD and COD during the bioremediation process. The results showed that the monooxygenase enzyme has activity between 0.257 and 3.859 U/mL. Meanwhile, the dioxygenase enzyme has activity between 0.579 and 5.402 U/mL. The final incubation compound profile found that Hexadecanoic acid methyl ester (C17H34O2) decreased by up to 47.66%. Moreover, BOD and COD reductions were 68.59% and 67.51%, respectively. This study concluded that oxygenation enzymes were produced to degrade pollutant compounds during the hydrocarbon bioremediation process. Further research is needed to improve the effectiveness of bioremediation by enhancing enzyme activity. Highlight Research The potential of hydrocarbon-degrading bacteria (Rhodococcus erythropolis and Pseudomonas aeruginosa) for pollutant degradation in the marine environment. Enzyme activity of hydrocarbon-degrading bacteria and degradation profile of pollutants. The dynamics of BOD and COD during the bioremediation process.
Analisis Dinamika Faktor Lingkungan Selama Proses Pengkristalan Garam di Greenhouse Salt Tunnel (Studi kasus di Pantai Utara dan Pantai Selatan, Jawat Timur, Indonesia) Abd Aziz Amin; Adi Tiya Yanuar; Zulkisam Pramudia; Yogita Ayu Dwi Susanti; Ilham Misbakudin AL Zamzami; Lutfi Ni’matus Salamah; Riski Agung Lestariadi; Lukman Hakim; Gatot Ardian; Mokh Hanifuddin; Andi Kurniawan
Jurnal Penelitian Pendidikan IPA Vol 11 No 4 (2025): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i4.7131

Abstract

Salt is an essential commodity for both domestic use and industrial applications, with East Java Province in Indonesia playing a crucial role in national salt production. Traditional salt production methods, primarily based on natural evaporation, face challenges due to seasonal factors, particularly during the rainy season. This study investigates the environmental conditions affecting the salt crystallization process in the Greenhouse Salt Tunnel (GST) technology. Field experiments were conducted at two sites, Tuban Regency on the North Coast and Malang Regency on the South Coast, where key parameters such as air temperature, water temperature, humidity, wind speed, and evaporation rates were monitored three times daily. The findings showed that water temperatures within the GST ranged from 31 to 38°C on the North Coast and 26 to 38°C on the South Coast. Wind speeds varied between 1.5–2.4 m/s on the North Coast and 0.4–1.0 m/s on the South Coast, while humidity levels ranged from 54 to 80%. The GST system achieved a salt production rate of 6.4 kg/m² with NaCl content exceeding 94%, a significant improvement over traditional methods, which yield less than 70% NaCl. These results demonstrate that GST technology enhances both the quality and quantity of salt production, though design modifications are needed to address the dynamic coastal environment.