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All Journal Current Biochemistry Jurnal Sumberdaya Hayati Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) JMM (Jurnal Masyarakat Mandiri) Jurnal Jejaring Matematika dan Sains Bubungan Tinggi: Jurnal Pengabdian Masyarakat IJOEP : International Journal of Ecophysiology Konservasi Hayati International Journal Of Science, Technology & Management (IJSTM) Bioactivities
Frans Grovy Naibaho
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Palangka Raya
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Transportation Other

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Antimicrobial Activity of Allium chinense G. Don. Frans Grovy Naibaho; Maria Bintang; Fachriyan Hasmi Pasaribu
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.
Analisis Molecular Docking Senyawa dari Jamur Endofit Bawang Dayak (Eleutherine bulbosa) Sebagai Inhibitor Lipase Pankreas Frans Grovy Naibaho; Ebrry Dwi Putra; Desimaria Panjaitan4; Vinsen Willi Wardhana
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (511.546 KB) | DOI: 10.33474/e-jbst.v7i2.484

Abstract

Nowadays some efforts are being made to reduce obesity by using drugs that aim to reduce appetite of the drug users. However, the use of drugs still has harmful side effects. Endophytic fungi originating from the Dayak onion plant are thought to have secondary metabolites that have potential as anti-obesity. The aims of this study were to determine the compounds produced by endophytic fungi of Dayak onion and to determine the potential components as inhibitors of pancreatic lipase enzymes. Samples of Dayak onions were collected from Palangka Raya using purposive sampling method. Isolation of endophytic fungi was conducted using surface sterilization method and the isolates were grown on PDA. Isolates were characterized and fermented on PDB for 14 days. The selected isolate was analyzed by using GCMS then molecular docking was performed using Autodock Vina and PyRx. The research results showed that there were 7 isolates of endophytic fungi isolated from leaves and roots tissues of Dayak onion. GCMS analysis showed that EBD1 contains 38 compounds. Molecular docking analysis showed that 1,3-Di-O-Acetylpentopyranose, a test ligand, has the closest binding affinity value (-6.6 kcal/mol) to orlistat (control) (-6.8 kcal/mol). Thus, it has the potential as an inhibitor of pancreatic lipase enzyme protein.
ISOLATION AND DEGRADATION STUDY ON CARBOSULFAN CONTAINING PESTICIDE OF BIOSUFACTANT-PRODUCING BACTERIA FROM BELAWAN SUMATERA Nunuk Priyani
International Journal of Ecophysiology Vol. 2 No. 02 (2020): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v2i02.4676

Abstract

Uncontrol use of synthetic pesticide causes environmental pollution and leaves its residue in soil and water. The objective of this research was to obtain bacterial isolates capable of producing biosurfactant from Belawan North Sumatera and to observe their biosurfactant activities as well as their potential in degrading of carbosulfan pesticide. Bacteria were isolated using selective medium, Bushnell-Hass Agar (BHA) containing 2% of pesticide with carbosulfan as the active compound. The result showed that all bacterial isolates were able to grow on the medium and consumed carbosulfan as the carbon sourc. The highest cell population was shown by isolate Sp.1 with total count was 7.6×109 CFU/ml. The highest biosurfactant activity was shown by isolate Sp.6 with the volume of emulsion was 5.627 cm3, meanwhile the highest production of biosurfactant shown by Sp.7 with concentration was 54.6 ppm. Two isolates, Sp.1 and Sp.6 were selected for further test to study their abilities in carbosulfan degradation. Both isolates were able to degrade carbosulfan completely after 21 days of incubation.
PELATIHAN KARAKTERISASI MORFOLOGI BAKTERI DAN FUNGI SEBAGAI PENGAYAAN PRAKTIKUM BIOLOGI BAGI GURU SEKOLAH MENENGAH ATAS Desimaria Panjaitan; Vinsen Willi Wardhana; Rizko Hadi; Faridah Tsuraya; Frans Grovy Naibaho
JMM (Jurnal Masyarakat Mandiri) Vol 7, No 1 (2023): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v7i1.12355

Abstract

Abstrak: Salah satu kendala pelaksanaan praktikum di Sekolah Menengah Atas adalah rendahnya pengetahuan dan keterampilan guru yang seringkali sekaligus berperan sebagai pengelola laboratorium. Sebagai salah satu solusi terhadap kendala tersebut, maka dilakukan kegiatan pengabdian kepada masyarakat yaitu pelatihan karakterisasi morfologi bakteri dan fungi bagi guru Sekolah Menengah Atas di kota Palangka Raya. Kegiatan dilaksanakan pada bulan Oktober 2022 dan diikuti oleh 6 orang guru biologi sebagai mitra sasaran. Metode yang digunakan adalah pemaparan materi mengenai prinsip dan teknik karakterisasi morfologi bakteri dan fungi, diskusi, praktik, serta monitoring dan evaluasi kegiatan berdasarkan analisis kuisioner sebelum dan sesudah pelaksanaan pelatihan. Praktik mencakup pengamatan morfologi bakteri dengan teknik pewarnaan sederhana dan pewarnaan Gram sedangkan morfologi fungi diamati dengan teknik pembuatan preparat segar. Hasil pelatihan menunjukkan adanya peningkatan pengetahuan dan keterampilan mitra sasaran melakukan teknik karakterisasi morfologi bakteri dan fungi (dari 16,7% menjadi 100%). Selain itu, seluruh mitra sasaran tertarik (100%) mengimplementasikan kegiatan praktikum karakterisasi morfologi bakteri dan fungi bagi para peserta didik di sekolahnya masing-masing. Abstract: As an effort to increase teacher knowledge and skills, community service activities was carried out, namely training in the morphological characterization of bacteria and fungi for biology teacher from several senior high school in Palangka Raya city. The activity was held in October 2022 and was attended by 6 biology teachers. The method used were the presentation of material regarding the principles and morphological characterization techniques of bacteria and fungi, discussion, practice, monitoring and evaluation by analyzing the result of questionnaire before and after the training. Practice included observing the morphology of bacteria with simple staining techniques and Gram staining while the morphology of fungi was observed using techniques for making fresh preparations. The results of the training showed an increase in the knowledge and skills of the target partners in carrying out the morphological characterization techniques of bacteria and fungi (from 16,7% to 100%). In addition, all target partners were also were interested (100%) in implementing practicum activities for the morphological characterization of bacteria and fungi for students in their respective schools.
Pelatihan Produksi Produk Pupuk Organik dan Maggot Kering sebagai Pakan Ternak dan Ikan pada Kelompok Tani Desimaria Panjaitan; Noverda Ayuchecaria; Frans Grovy Naibaho; Mirnawati Dewi
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 5, No 4 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v5i4.10106

Abstract

Para petani di Kelurahan Kalampangan  telah melakukan budidaya Black Soldier Fly (BSF) untuk mengatasi masalah sampah organik sisa perkebunan dan pertanian. Hasil budidaya BSF juga dapat dikembangkan menjadi produk pupuk organik dan pakan ternak yang merupakan solusi dari terus meningkatnya harga pupuk dan pakan ternak kimiawi. Tujuan kegiatan pengabdian kepada masyarakat ini adalah untuk memberikan pelatihan produksi produk pupuk organik dan pakan ternak berbasis budidaya maggot kepada para kelompok tani di Kelurahan Kalampangan. Kegiatan dilaksanakan pada bulan Agustus 2023 di Kantor Badan Penyuluh Pertanian (BPP) Kelurahan Kalampangan Kota Palangka Raya. Kegiatan diikuti oleh 71 orang peserta yang didominasi oleh para petani dan sebagian lainnya merupakan perwakilan anggota PKK, masyarakat dan perangkat pemerintah setempat. Kegiatan pengabdian ini diawali dengan dilakukannya koordinasi dan persiapan kegiatan dengan perangkat kelurahan. Pelaksanaan kegiatan dilakukan dengan pembekalan peserta dengan buku saku materi, pemaparan materi pelatihan, demonstrasi pembuatan produk pupuk organik dan pakan ternak berbasis maggot BSF, dan sesi diskusi. Peserta kegiatan juga diminta untuk mengisi kuisioner pre-test dan post-test untuk menilai keberhasilan terlaksananya pelatihan. Hasil kegiatan menunjukkan tercapainya tujuan kegiatan karena adanya peningkatan pengetahuan dan keterampilan peserta berdasarkan perbandingan nilai rata-rata kuisioner pre-test dan post-test yaitu dari 55,83% menjadi 82,55%.Farmers in Kalampangan have cultivated Black Soldier Fly (BSF) to overcome the problem of organic waste left over from plantations and agriculture. The results of BSF cultivation can also be developed into organic fertilizer and animal feed products, which are a solution to the increasing prices of chemical fertilizers and pellets. This activity aims to provide training in producing organic fertilizer products and animal feed based on BSF cultivation. Activities were carried out in August 2023 at the BPP Kalampangan Village, Palangka Raya City. This activity was attended by 71 participants, dominated by local farmer groups, and the rest were members of PKK, local people, and officials. This service activity began with coordinating and preparing activities with sub-district officials. The activities were carried out by providing participants with material pocketbooks, presenting training materials, demonstrating how to make organic fertilizer and animal feed products based on BSF maggots, and holding discussion sessions. Activity participants were also asked to fill out pre-test and post-test questionnaires. The results showed an increase in participants' knowledge and skills based on comparing the average scores of the pre-test and post-test questionnaires, namely from 55.83% to 82.55%.
Isolasi Bakteri Penghasil Biosurfaktan Menggunakan Media yang Mengandung Pestisida Karbosulfan: Isolation of Biosurfactant-Producing Bacteria Using Medium Containing Carbosulfan Pesticide Naibaho, Frans Grovy; Priyani, Nunuk; Munir, Erman; Damanik, Nina Septania
Jurnal Jejaring Matematika dan Sains Vol. 2 No. 1 (2020): Edisi Oktober 2020
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/jjms.2020.v2.i1.345

Abstract

Penggunaan pestisida sintetik yang tidak terkontrol dapat menyebabkan pencemaran lingkungan karena meningggalkan senyawa pestisida di tanah dan perairan. Bioremediasi adalah salah satu cara untuk mengurangi cemaran senyawa kimia berbahaya di lingkungan dengan menggunakan mikroorganisme. Tujuan penelitian ini ialah untuk memperoleh isolat bakteri potensial penghasil biosurfaktan dari laut Belawan Sumatera Utara dan untuk mengetahui aktivitas biosurfaktannya. Isolasi bakteri dilakukan dengan menggunakan media selektif Bushnell-Hass Agar (BHA) yang mengandung 12 ppm pestisida berbahan aktif karbosulfan sebagai sumber karbon. Aktivitas biosurfaktan dilakukan dengan metode Drop collapsing test dan konsentrasi biosurfaktan dilakukan dengan metose orsinol yang dimodifikasi. Hasil penelitian menunjukkan 9 isolat berhasil diisolasi dan mampu tumbuh pada media yang mengandung karbosulfan. Aktivitas biosurfaktan tertinggi dihasilkan oleh isolat FB6 yang ditandai dengan pembentukan emulsi sebesar 5,627 mL dan produksi biosurfaktan tertinggi ditunjukkan oleh isolat FB7 dengan konsentrasi 54,6 ppm.
Antifungal Activity of Endophytic Bacteria Isolated from Dayak Onion (Eleutherine bulbosa) Against Candida albicans Krestina, Widya; Mutiasari, Dian; Wardhana, Vinsen Willi; Zulviana; Rahmawati, Maynissa; Naibaho, Frans Grovy; Panjaitan, Desimaria
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.157-161

Abstract

Candidiasis is a fungal infection due to a decrease in the immune system caused by Candida spp. In general, candidiasis is treated with antifungal drugs, but uncontrolled use can result in the emergence of drug resistance and toxicity. Endophytic bacteria have the potential to be biological control agents against various fungal infections including candidiasis. This study aims to determine the antagonistic activity of endophytic bacteria isolated from Dayak onions and the antifungal activity of its cell-free supernatant (CFS) against Candida albicans. Thirteen isolate strains of endophytic bacteria were used in this study. Antagonistic activity was tested using the agar plug diffusion method. Endophytic bacteria that showed antagonistic activity against C. albicans were cultivated in Nutrient Broth media to produce CFS. The antifungal activity of CFS was tested using the Kirby–Bauer disk diffusion method. The results indicated that six isolates of endophytic bacteria from dayak onions have antagonistic activity against C. albicans. CFS from strain CED4 has a strong antifungal activity against C. albicans with a clear zone diameter of 18.5 mm. Further studies are needed to determine the antifungal compounds produced by CED4 isolates for the development of anticandidal.
Evaluation of Antagonistic Activity of Endophytic Bacteria from Dayak Onion Against Ganoderma boninense Desiana Sinta; Norwahyunie Norwahyunie; Noraini Noraini; Zulviana Zulviana; Frans Grovy Naibaho; Vinsen Willi Wardhana; Desimaria Panjaitan; Adrian Hartanto
Bioactivities Vol. 2 No. 2 (2024): Bioactivities
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/bioactivities.2963-654X.239

Abstract

Basal stem rot (BSR) is one of the key contributors to the decline in palm oil production in Indonesia. BSR is caused by the pathogenic plant fungus Ganoderma boninense. Currently, the control of G. boninense growth relies on systemic fungicides. However, systemic fungicides have negative impacts as they can harm natural soil microorganisms. Biocontrol agents present an environmentally friendly alternative for G. boninense management. The objective of this research was to select and identify endophytic bacterial isolates from Dayak onion [Eleutherine bulbosa (Mill.) Urb.] with antagonistic activity against G. boninense. The investigation was initiated with the isolation of 34 endophytic bacterial isolates and G. boninense isolate. The hemolytic capabilities of endophytic bacterial isolates were evaluated on blood agar media. Antagonism assessments were conducted through the dual-culture method, and the inhibition of G. boninense was measured using the percentage inhibition of radial growth (PIRG). The endophytic bacteria exhibiting the highest PIRG values were identified through molecular analysis based on the 16S rRNA gene sequence. Results revealed that 27 isolates demonstrated no ability to hydrolyze blood agar (gamma hemolysis) indicating no pathogenicity. Isolate CED9 demonstrated the highest PIRG value at 70.26%. Genomic identification based on the 16S rRNA gene sequence confirmed that isolate CED9 was classified as Bacillus subtilis. The identification of biocontrol agents from Dayak onion represents valuable information and a potential arsenal for future strategies in mitigating (BSR) disease.
Keanekaragaman Jamur Makroskopis di Hutan Gambut Arboretum Nyaru Menteng Kalimantan Tengah Desimaria Panjaitan; Frans Grovy Naibaho; Retno Agnestisia; Ivan Permana Putra
Konservasi Hayati Vol 19 No 2 (2023): OKTOBER
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i2.29404

Abstract

Information on macroscopic fungi from Central Kalimantan is still limited compared to other regions in Indonesia. Arboretum Nyaru Menteng, a conservation area located in Central Kalimantan, poses many macrofungi due to its favorable environmental conditions that support the growth of various types of macrofungi. However, the diversity of macroscopic fungi from Arboretum Nyaru Menteng has never been reported. Thus, the current works aimed to provide initial information on the diversity of macroscopic fungi in this conservation area. The exploration was done using the opportunistic sampling method. Fruiting bodies were documented, collected, and identified based on macroscopic characteristics. Four environmental microclimate variables were measured in four random sampling locations: temperature, light intensity, air humidity, and soil acidity. The results confirmed that there were 27 species of macrofungi belonging to 18 genera and 14 families. Polyporaceae was the most dominant family found in this research. Most fungi grew on a dead log while the others were recognized as mycorrhizal fungi and parasites on plants. The environmental measurements showed that Arboretum Nyaru Menteng has an optimal habitat for recorded fungi growth. The record of macrofungi diversity in this research contributes to Indonesia mycoflora, particularly from Central Kalimantan.
Trends and Knowledge Gaps In Fish-Based Fermented Foods: A Bibliometric Analysis From The Last Decade (2015-2024) Mamangkey, Jendri; Grovy Naibaho, Frans; Rosenta Purba, Ika; Yusriya Hibatullah, Alnadia; Aryanti Pada Soa, Christina
International Journal of Science, Technology & Management Vol. 6 No. 6 (2025): November 2025
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v6i6.1369

Abstract

Fish-based fermented foods are integral to traditional diets across Asia, Africa, and Europe, valued for both their nutritional role and cultural significance. Increasingly, they are also studied for potential functional properties such as probiotic activity and bioactive compounds, yet their global research landscape has not been systematically assessed. This study presents a bibliometric analysis of global research on fish-based fermented foods published between 2015 and 2024. Data were retrieved from the Scopus database and analyzed using the Bibliometrix R package and Biblioshiny interface. A total of 263 documents were identified across 145 sources, authored by 1,150 researchers. The field has shown rapid growth, with an annual publication increase of 27.01% and an average of 28.48 citations per article, indicating strong scholarly visibility. Most contributions originated from East and Southeast Asia, particularly China, South Korea, and Thailand, reflecting the cultural and culinary importance of fermented fish in these regions. Author productivity followed Lotka’s law, with a small group of core researchers driving sustained output. Keyword co-occurrence and thematic mapping revealed dominant clusters around fermented fish sauce, lactic acid bacteria, and microbial diversity, alongside emerging themes related to fatty acids and bacterial communities. Despite these advances, the analysis highlighted notable gaps, including limited international collaboration and underrepresentation of research from Africa and parts of South Asia. These findings suggest that while the field is expanding and diversifying, greater cross-regional engagement and methodological innovation are needed to fully explore the nutritional, functional, and socio-economic dimensions of fish-based fermented foods.
Co-Authors Adrian Hartanto Aryanti Pada Soa, Christina Ayuchecaria, Noverda Damanik, Nina Septania Desiana Sinta Desimaria Panjaitan Desimaria Panjaitan Dian Mutiasari, Dian Ebrry Dwi Putra Erman Munir Fachriyan Hasmi Pasaribu Faridah Tsuraya Hibatullah, Alnadia Yusriya Ivan Permana Putra Jendri Mamangkey Krestina, Widya MARIA BINTANG Mirnawati Dewi Nina Septania Damanik Noraini Noraini Norwahyunie Norwahyunie NUNUK PRIYANI Nunuk Priyani Rahmawati, Maynissa Retno Agnestisia Rizko Hadi Rosenta Purba, Ika Vinsen Willi Wardhana Zulviana Zulviana Zulviana