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Analisis Molecular Docking Senyawa dari Jamur Endofit Bawang Dayak (Eleutherine bulbosa) Sebagai Inhibitor Lipase Pankreas Frans Grovy Naibaho; Ebrry Dwi Putra; Desimaria Panjaitan4; Vinsen Willi Wardhana
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (511.546 KB) | DOI: 10.33474/e-jbst.v7i2.484

Abstract

Nowadays some efforts are being made to reduce obesity by using drugs that aim to reduce appetite of the drug users. However, the use of drugs still has harmful side effects. Endophytic fungi originating from the Dayak onion plant are thought to have secondary metabolites that have potential as anti-obesity. The aims of this study were to determine the compounds produced by endophytic fungi of Dayak onion and to determine the potential components as inhibitors of pancreatic lipase enzymes. Samples of Dayak onions were collected from Palangka Raya using purposive sampling method. Isolation of endophytic fungi was conducted using surface sterilization method and the isolates were grown on PDA. Isolates were characterized and fermented on PDB for 14 days. The selected isolate was analyzed by using GCMS then molecular docking was performed using Autodock Vina and PyRx. The research results showed that there were 7 isolates of endophytic fungi isolated from leaves and roots tissues of Dayak onion. GCMS analysis showed that EBD1 contains 38 compounds. Molecular docking analysis showed that 1,3-Di-O-Acetylpentopyranose, a test ligand, has the closest binding affinity value (-6.6 kcal/mol) to orlistat (control) (-6.8 kcal/mol). Thus, it has the potential as an inhibitor of pancreatic lipase enzyme protein.
PENCEGAHAN DEMAM BERDARAH MENGGUNAKAN LILIN ANTI NYAMUK Stevin Carolius Angga; Erwin Prasetya Toepak; Desimaria Panjaitan; Ika Oktavia Wulandari; Agus Lasono; Sari Namarito Simamarta; Sudarman Rahman
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 5 No 2 (2021)
Publisher : Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas Kampus Limau Manis - Padang, Sumatera Barat Indonesia-25163

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/logista.5.2.82-86.2021

Abstract

Penyakit demam berdarah merupakan masalah kesehatan masyarakat di Indonesia terutama pada musim penghujan. Penyakit ini ditularkan oleh virus dengue melalui nyamuk Aedes aegypti. Provinsi Kalimantan tengah menempati urutan tiga besar dalam angka penyakit demam berdarah. Salah satu penangangan yang dapat dilakukan untuk melakukan pencegahan demam berdarah adalah dengan memanfaatkan bahan alam yaitu ekstrak dari serai (Cymbopogon citratus) atau serai wangi (Cymbopogon nardus). Pada umumnya serai digunakan sebagai bumbu dapur, sementara serai wangi tidak terlalu dimanfaatkan. Memperhatikan hal-hal tersebut, maka pembuatan lilin anti nyamuk dari ekstrak serai wangi dapat menjadi salah satu solusi dalam mengatasi penyakit demam berdarah dan pengetahuan ini patut disosialisasikan kepada masyarakat. Metode yang digunakan dalam sosialisasi ini berupa ceramah dan pembagian sampel lilin anti nyamuk. Sementara materi ceramah yang disosialisasikan adalah tentang bahaya penyakit demam berdarah, pencegahannya, pemanfaatan serai wangi sebagai alternatif pencegahan demam berdarah dalam bentuk lilin, teknik pembuatan lilin anti nyamuk. Kegiatan ini diharapkan dapat membantu pemerintah dan masyarakat desa Tumbang Nusa Kabupaten Pulang Pisau dalam penanganan penyakit demam berdarah. Selain itu, kegiatan ini diharapkan dapat meningkatkan kesadaran masyarakat tentang kesehatan dalam mencegah penyakit deman berdarah. Kata kunci: sosialisasi, demam berdarah, serai wangi, lilin anti nyamuk, minyak atsiri ABSTRACT Dengue fever disease is a public health problem in Indonesia, especially during the rainy season. This disease is transmitted by the dengue virus through Aedes aegypti mosquito. Central Kalimantan is the top three in terms of dengue fever disease. Meanwhile, there are Kalimantan natural products which can be used as repellent to prevent the mosquito, such as extracts from lemongrass (Cymbopogon citratus) or citronella grass (Cymbopogon nardus). Mostly lemongrass is used as a kitchen spice, while citronella grass is not very used generally. Furthermore, the utilization of mosquito repellent candles from citronella extract could be a solution to overcome dengue fever disesase. The method used in this socialization is in the form of lectures and distribution of mosquito repellent candle samples. While the lecture material that was socialized was about the dangers of dengue fever, its prevention, the use of citronella grass as an alternative to dengue fever prevention in the form of candles, and preparation of mosquito repellent candles. This socialization is expected to supports the government and the community of Tumbang Nusa village, Pulang Pisau Regency to overcome the dengue fever disease. In addition, this activity is expected to increase public awareness about health in preventing dengue fever. Keywords: socialization, dengue fever, citronella grass, mosquito repellent candle, essential oil
Keanekaragaman Jamur Makroskopis di Kawasan Hutan Universitas Palangka Raya Kalimantan Tengah Desimaria Panjaitan; Vinsen Willi Wardhana; Santi Dwi Febiolasari
Jurnal Kajian Ilmiah Vol. 22 No. 2 (2022): Mei 2022
Publisher : Lembaga Penelitian, Pengabdian Kepada Masyarakat dan Publikasi (LPPMP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.31 KB) | DOI: 10.31599/jki.v22i2.1145

Abstract

Macroscopic fungi comprise a very diverse group of heterotrophic and mostly saprophytic organisms. This research purpose was to identify the diversity of macroscopic fungi from the forest of University of Palangka Raya. The fungi samples were collected from May until June 2021 using purposive sampling method. The samples were identified based on macroscopic characters such as the form of fruiting bodies, lamellae, cap shape and colours. There were 18 species of Basidiomycota which consist of 9 famili and 12 genera. The species were identified as Agrocybe sp., Auricularia sp.1, Auricularia sp.2, Collybia sp., Dacryopinax spathularia, Ganoderma sp.1, Ganoderma sp.2, Ganoderma sp.3, Hygrocybe sp., Marasmiellus sp., Mycena sp.1, Mycena sp.2, Pycnoporus sanguineus, Schizophyllum commune, Trametes sp.1, Trametes sp.2, Trametes sp.3, and Tyromyces sp. Descriptions of each species were provided.
PELATIHAN KARAKTERISASI MORFOLOGI BAKTERI DAN FUNGI SEBAGAI PENGAYAAN PRAKTIKUM BIOLOGI BAGI GURU SEKOLAH MENENGAH ATAS Desimaria Panjaitan; Vinsen Willi Wardhana; Rizko Hadi; Faridah Tsuraya; Frans Grovy Naibaho
JMM (Jurnal Masyarakat Mandiri) Vol 7, No 1 (2023): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v7i1.12355

Abstract

Abstrak: Salah satu kendala pelaksanaan praktikum di Sekolah Menengah Atas adalah rendahnya pengetahuan dan keterampilan guru yang seringkali sekaligus berperan sebagai pengelola laboratorium. Sebagai salah satu solusi terhadap kendala tersebut, maka dilakukan kegiatan pengabdian kepada masyarakat yaitu pelatihan karakterisasi morfologi bakteri dan fungi bagi guru Sekolah Menengah Atas di kota Palangka Raya. Kegiatan dilaksanakan pada bulan Oktober 2022 dan diikuti oleh 6 orang guru biologi sebagai mitra sasaran. Metode yang digunakan adalah pemaparan materi mengenai prinsip dan teknik karakterisasi morfologi bakteri dan fungi, diskusi, praktik, serta monitoring dan evaluasi kegiatan berdasarkan analisis kuisioner sebelum dan sesudah pelaksanaan pelatihan. Praktik mencakup pengamatan morfologi bakteri dengan teknik pewarnaan sederhana dan pewarnaan Gram sedangkan morfologi fungi diamati dengan teknik pembuatan preparat segar. Hasil pelatihan menunjukkan adanya peningkatan pengetahuan dan keterampilan mitra sasaran melakukan teknik karakterisasi morfologi bakteri dan fungi (dari 16,7% menjadi 100%). Selain itu, seluruh mitra sasaran tertarik (100%) mengimplementasikan kegiatan praktikum karakterisasi morfologi bakteri dan fungi bagi para peserta didik di sekolahnya masing-masing. Abstract: As an effort to increase teacher knowledge and skills, community service activities was carried out, namely training in the morphological characterization of bacteria and fungi for biology teacher from several senior high school in Palangka Raya city. The activity was held in October 2022 and was attended by 6 biology teachers. The method used were the presentation of material regarding the principles and morphological characterization techniques of bacteria and fungi, discussion, practice, monitoring and evaluation by analyzing the result of questionnaire before and after the training. Practice included observing the morphology of bacteria with simple staining techniques and Gram staining while the morphology of fungi was observed using techniques for making fresh preparations. The results of the training showed an increase in the knowledge and skills of the target partners in carrying out the morphological characterization techniques of bacteria and fungi (from 16,7% to 100%). In addition, all target partners were also were interested (100%) in implementing practicum activities for the morphological characterization of bacteria and fungi for students in their respective schools.
Uji In Vitro Aktivitas Antibakteri Ekstrak Bawang Dayak (Eleutherine bulbosa) Terhadap Isolat Klinis Frans Grovy Naibaho; Anita Maulina; Liswara Neneng
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 1 (2023): Agustus 2023
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i1.542

Abstract

Dayak onion (Eleutherine bulbosa) is a medicinal plant that has been known and used by Dayak People from Central Kalimantan. It contains various chemical compounds such as alkaloids, glycosides, flavonoids, phenolics, steroids, and tannins which are known to have antibacterial effects. This study aimed to determine the antibacterial activity of Dayak onion extract against two pathogenic bacteria namely Escherichia coli and Staphylococcus aureus in vitro and to determine the minimum inhibitory concentration (MIC) value of the Dayak onion extract. Extraction was carried out by maceration method using ethanol 96 % and n-hexane. The antibacterial activity test was carried out using the disc diffusion method (Kirby-Bauer Test). The determination of MIC value was carried out by using disc diffusion with five different concentrations 500, 250, 100, 50, 25, and 10 mg/mL. The research result showed that Dayak onion had antibacterial activity. Ethanol 96% extract was only able to inhibit the growth of S. aureus with an inhibition zone of 15.02 mm. Meanwhile, the n-hexane extract was able to inhibit the growth of S. aureus and E. coli with an inhibition zone of 18.48 dan 14.94 mm, respectively. MIC value of ethanol 96% for S. aureus was 50 mg/mL. N-hexane extract has MIC value for E. coli and S. aureus was 10 mg/mL.
Pelatihan Produksi Produk Pupuk Organik dan Maggot Kering sebagai Pakan Ternak dan Ikan pada Kelompok Tani Desimaria Panjaitan; Noverda Ayuchecaria; Frans Grovy Naibaho; Mirnawati Dewi
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 5, No 4 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v5i4.10106

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Para petani di Kelurahan Kalampangan  telah melakukan budidaya Black Soldier Fly (BSF) untuk mengatasi masalah sampah organik sisa perkebunan dan pertanian. Hasil budidaya BSF juga dapat dikembangkan menjadi produk pupuk organik dan pakan ternak yang merupakan solusi dari terus meningkatnya harga pupuk dan pakan ternak kimiawi. Tujuan kegiatan pengabdian kepada masyarakat ini adalah untuk memberikan pelatihan produksi produk pupuk organik dan pakan ternak berbasis budidaya maggot kepada para kelompok tani di Kelurahan Kalampangan. Kegiatan dilaksanakan pada bulan Agustus 2023 di Kantor Badan Penyuluh Pertanian (BPP) Kelurahan Kalampangan Kota Palangka Raya. Kegiatan diikuti oleh 71 orang peserta yang didominasi oleh para petani dan sebagian lainnya merupakan perwakilan anggota PKK, masyarakat dan perangkat pemerintah setempat. Kegiatan pengabdian ini diawali dengan dilakukannya koordinasi dan persiapan kegiatan dengan perangkat kelurahan. Pelaksanaan kegiatan dilakukan dengan pembekalan peserta dengan buku saku materi, pemaparan materi pelatihan, demonstrasi pembuatan produk pupuk organik dan pakan ternak berbasis maggot BSF, dan sesi diskusi. Peserta kegiatan juga diminta untuk mengisi kuisioner pre-test dan post-test untuk menilai keberhasilan terlaksananya pelatihan. Hasil kegiatan menunjukkan tercapainya tujuan kegiatan karena adanya peningkatan pengetahuan dan keterampilan peserta berdasarkan perbandingan nilai rata-rata kuisioner pre-test dan post-test yaitu dari 55,83% menjadi 82,55%.Farmers in Kalampangan have cultivated Black Soldier Fly (BSF) to overcome the problem of organic waste left over from plantations and agriculture. The results of BSF cultivation can also be developed into organic fertilizer and animal feed products, which are a solution to the increasing prices of chemical fertilizers and pellets. This activity aims to provide training in producing organic fertilizer products and animal feed based on BSF cultivation. Activities were carried out in August 2023 at the BPP Kalampangan Village, Palangka Raya City. This activity was attended by 71 participants, dominated by local farmer groups, and the rest were members of PKK, local people, and officials. This service activity began with coordinating and preparing activities with sub-district officials. The activities were carried out by providing participants with material pocketbooks, presenting training materials, demonstrating how to make organic fertilizer and animal feed products based on BSF maggots, and holding discussion sessions. Activity participants were also asked to fill out pre-test and post-test questionnaires. The results showed an increase in participants' knowledge and skills based on comparing the average scores of the pre-test and post-test questionnaires, namely from 55.83% to 82.55%.
Increasing the Knowledge of High School Biology Teachers on the Preservation of Pediculus humanus capitis in Preparat Glass Fandi Tuju; Mirnawati Dewi; Febri Nur Ngazizah; Desimaria Panjaitan; Decenly Decenly
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 6, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v6i2.10394

Abstract

Pediculus humanus capitis (head lice) is an obligate ectoparasite. Preparation of specimens is a procedure carried out to accurately preserve, identify, and analyze head lice. The Palangka Raya Biology Teachers Forum (MGMP) has collaborated with the Community Service Team of the Biology Study Program. This activity aims to improve high school Biology teachers' skills in preserving Pediculus humanus capitis (morphological observation and identification). This provides a solution to make school laboratory practicals more attractive, as preserved specimens can be stored and used long-term. The activity was offline at the PPIIG Building Laboratory, Palangka Raya University, in August 2023. The activity included lectures, discussions, training, and pre-test and post-test evaluation. The evaluation results show an increase in scores after the training, with an average pre-test score of 50.00 increasing to 80.33 in the post-test. It can be concluded that participants' knowledge increased after attending this training.
Oral Klebsiella sp. Involved in Dental Caries: A Case of Individuals Gargling with Peatwater Turnip, Oktaviani Naulita; Frethernety, Agnes; Hanasia; Panjaitan, Desimaria; Damayanti, Elvina; Rovik, Anwar; Malik, Handayani
Jurnal Sumberdaya Hayati Vol. 10 No. 3 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.3.122-127

Abstract

Most of the microbes inhabiting the oral cavities are harmless but can cause disturbance and discomfort if the microbial population increases significantly. One of the oral disorders that is often experienced by children, is dental caries. Dental caries is causedby various factors, including eating and brushing habits. The people of Central Kalimantan who live in the watershed use peat water in their daily activities, including for gargling and brushing their teeth. The acidic characteristics of peat water and the high content of organic substances trigger the development of microbes that cause dental caries.This study used dental caries swab samples of patients who rinsed their mouths with peat water. Bacteria were identified molecularly with 16SrRNA markers with primers 63F and 1387R. The similarity of nucleotide sequences was analyzed with BLAST on GeneBank. A phylogenetic tree was built with Maximum Likelihood. Both isolates have a fairly high level of similarity to Klebsiella pneumoniae, namely 91% and 93%. Both isolates are in the same clade, Klebsiella spp., and are close to various commonly found bacteria in the oral cavity, such as Streptococcus mutans and Lactobacillus salivarius.
Antifungal Activity of Endophytic Bacteria Isolated from Dayak Onion (Eleutherine bulbosa) Against Candida albicans Krestina, Widya; Mutiasari, Dian; Wardhana, Vinsen Willi; Zulviana; Rahmawati, Maynissa; Naibaho, Frans Grovy; Panjaitan, Desimaria
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.157-161

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Candidiasis is a fungal infection due to a decrease in the immune system caused by Candida spp. In general, candidiasis is treated with antifungal drugs, but uncontrolled use can result in the emergence of drug resistance and toxicity. Endophytic bacteria have the potential to be biological control agents against various fungal infections including candidiasis. This study aims to determine the antagonistic activity of endophytic bacteria isolated from Dayak onions and the antifungal activity of its cell-free supernatant (CFS) against Candida albicans. Thirteen isolate strains of endophytic bacteria were used in this study. Antagonistic activity was tested using the agar plug diffusion method. Endophytic bacteria that showed antagonistic activity against C. albicans were cultivated in Nutrient Broth media to produce CFS. The antifungal activity of CFS was tested using the Kirby–Bauer disk diffusion method. The results indicated that six isolates of endophytic bacteria from dayak onions have antagonistic activity against C. albicans. CFS from strain CED4 has a strong antifungal activity against C. albicans with a clear zone diameter of 18.5 mm. Further studies are needed to determine the antifungal compounds produced by CED4 isolates for the development of anticandidal.
Keanekaragaman Jenis Mangga (Mangifera spp.) di Tumbang Talaken Kecamatan Manuhing Kabupaten Gunung Mas Kalimantan Tengah Satriani, Inriyati; Panjaitan, Desimaria; Sunariyati, Siti
Journal of Biotropical Research and Nature Technology Vol. 2 No. 2 (2024): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i2.11387

Abstract

Kalimantan adalah salah satu pusat keanekaagaman jenis mangga (Mangifera spp.) di Indonesia. Aktivitas antropogenik dan pembukaan lahan agrikultur di Kalimantan Tengah termasuk di Tumbang Talaken Kecamatan Manuhing Kabupaten Gunung Mas dikhawatirkan dapat mengancam keanekaragaman hayati tumbuh-tumbuhan Mangifera spp. di kawasan ini. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis mangga (Mangifera spp.) di Tumbang Talaken Kecamatan Manuhing Kabupaten Gunung Mas Kalimantan Tengah. Penelitian ini menggunakan metode jelajah dan pengambilan sampel menggunakan teknik purposive sampling. Sampel tumbuhan mangga dikoleksi, didokumentasi dan diidentifikasi berdasarkan karakter morfologinya. Analisis data dilakukan secara deskriptif kualitatif. Hasil penjelajahan ditemukan total 103 individu pohon mangga di Tumbang Talaken yang tersebar di pekarangan rumah warga sebanyak 76 individu dan di hutan alami sebanyak 27 individu. Hasil identifikasi diperoleh 10 jenis mangga yaitu Mangifera applanata (Lipis), Mangifera caesia (Binjai), M. casturi (Kasturi), M. foetida (Asem gayung), M. indica (Mangga), M. laurina (Hampalam), M. odorata (Kwini), M. pajang (Asem pangi), Mangifera similis (Rarawa) dan Mangifera torquenda (Asem puter).