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Pathogenicity of Endophytic Fusarium oxysporum Isolated from Weeds in Banana Plantations against Bananas Seedlings var. Raja Bulu Vinsen Willi Wardhana; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 17 No 1 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.1.1-8

Fusarium oxysporum merupakan penyebab penyakit layu dan banyak mematikan tanaman pisang. Cendawan ini diketahui mampu hidup dan bertahan sebagai endofit pada gulma dan menjadi sumber inokulum yang nantinya menyebabkan penyakit pada tanaman pisang. Penelitian ini dilakukan untuk mengisolasi F. oxysporum dari akar gulma yang tumbuh di sekitar pertanaman pisang di Bogor, Jawa Barat, kemudian menguji sifat patogenisitasnya. Identifikasi morfologi dilakukan dengan mengamati koloni, konidium, dan klamidosporanya. Uji patogenisitas isolat endofit dilakukan di laboratorium menggunakan bibit pisang var. Raja Bulu. Sebanyak 9 isolat F. oxysporum berhasil diisolasi dari gulma yang tumbuh di sekitar pertanaman pisang. Semua isolat menunjukkan ciri morfologi sebagai F. oxysporum, yaitu koloni berwarna ungu, makrokonidium berbentuk kano dengan tiga sekat false head pendek, mikrokonidium berbentuk silindris atau ginjal, dan klamidospora umumnya tersusun tunggal. Semua isolat menyebabkan insidensi penyakit sebesar 100% pada bibit pisang var. Raja Bulu dengan keparahan gejala layu pada daun antara 58.33% sampai 77.78% dan nekrosis pada bonggolnya antara 50.00% sampai 69.44%.

Analisis Molecular Docking Senyawa dari Jamur Endofit Bawang Dayak (Eleutherine bulbosa) Sebagai Inhibitor Lipase Pankreas Frans Grovy Naibaho; Ebrry Dwi Putra; Desimaria Panjaitan4; Vinsen Willi Wardhana
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Nowadays some efforts are being made to reduce obesity by using drugs that aim to reduce appetite of the drug users. However, the use of drugs still has harmful side effects. Endophytic fungi originating from the Dayak onion plant are thought to have secondary metabolites that have potential as anti-obesity. The aims of this study were to determine the compounds produced by endophytic fungi of Dayak onion and to determine the potential components as inhibitors of pancreatic lipase enzymes. Samples of Dayak onions were collected from Palangka Raya using purposive sampling method. Isolation of endophytic fungi was conducted using surface sterilization method and the isolates were grown on PDA. Isolates were characterized and fermented on PDB for 14 days. The selected isolate was analyzed by using GCMS then molecular docking was performed using Autodock Vina and PyRx. The research results showed that there were 7 isolates of endophytic fungi isolated from leaves and roots tissues of Dayak onion. GCMS analysis showed that EBD1 contains 38 compounds. Molecular docking analysis showed that 1,3-Di-O-Acetylpentopyranose, a test ligand, has the closest binding affinity value (-6.6 kcal/mol) to orlistat (control) (-6.8 kcal/mol). Thus, it has the potential as an inhibitor of pancreatic lipase enzyme protein.

Exploration of pitcher plants in University of Palangka Raya Muhammad Rizki; Vinsen Willi Wardhana Wardhana; Mawardin Mawardin; Siti Sunariyati
BIOEDUSCIENCE Vol 5 No 2 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: This study is a pilot project on the effectiveness of flora, especially the type of semar bag (Nepenthes sp) found at the University of Palangka Raya (UPR). The research aims to identify semar bag plants at the University of Palangka Raya. The initial observations found at least more than one type of semar bag that lives in the peat forest of the UPR campus. The study was conducted in August-November 2020. Methods: Semar bag diversity data is collected by cruising methods. The data was analyzed using literature studies to be further identified using a sealed bag identification manual. Results: The study found three species of semar bags found in the forest campus of Palangka Raya University. The bag is a type of Nepenthes mirabilis (Lour.) Druce, Nepenthes gracilis Korth., and Nepenthes rafflesiana Jack. Morphological characters that distinguish these three species are the morphology of leaves and pouches. The range of environmental parameter values is air temperature 28-38oC, medium-open coverage, humidity 62-98%, and soil pH 5-7.5. Conclusions: This study's results are expected to be a database of flora biodiversity in Central Kalimantan.

Keanekaragaman Jamur Makroskopis di Kawasan Hutan Universitas Palangka Raya Kalimantan Tengah Desimaria Panjaitan; Vinsen Willi Wardhana; Santi Dwi Febiolasari
Jurnal Kajian Ilmiah Vol. 22 No. 2 (2022): Mei 2022
Publisher : Lembaga Penelitian, Pengabdian Kepada Masyarakat dan Publikasi (LPPMP)

Macroscopic fungi comprise a very diverse group of heterotrophic and mostly saprophytic organisms. This research purpose was to identify the diversity of macroscopic fungi from the forest of University of Palangka Raya. The fungi samples were collected from May until June 2021 using purposive sampling method. The samples were identified based on macroscopic characters such as the form of fruiting bodies, lamellae, cap shape and colours. There were 18 species of Basidiomycota which consist of 9 famili and 12 genera. The species were identified as Agrocybe sp., Auricularia sp.1, Auricularia sp.2, Collybia sp., Dacryopinax spathularia, Ganoderma sp.1, Ganoderma sp.2, Ganoderma sp.3, Hygrocybe sp., Marasmiellus sp., Mycena sp.1, Mycena sp.2, Pycnoporus sanguineus, Schizophyllum commune, Trametes sp.1, Trametes sp.2, Trametes sp.3, and Tyromyces sp. Descriptions of each species were provided.

ANALISIS VEGETASI HABITAT NEPENTHES sp. DI HUTAN GAMBUT KAMPUS UNIVERSITAS PALANGKA RAYA, KALIMANTAN TENGAH Siti Sunariyati; Vinsen Willi Wardhana; Muhammad Rizki
Jurnal Biosilampari : Jurnal Biologi Vol 5 No 1 (2022): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v5i1.1849

This research is a continuation of previous research on the diversity of pitcher plants (Nepenthes sp) at the University of Palangka Raya. This study aims to determine the habitat characteristics of the pitcher plant (Nepenthes sp) at the University of Palangka Raya. We carried out this research in June-September 2022. The habitat characteristics of pitcher plants were collected by purposive sampling to analyze habitat characteristics data using a quadratic sampling method consisting of two transects with 15 plots based on the findings of Nepenthes sp. at the research site. The first transect is in a secondary forest, and the second transect is in the scrub with slightly open conditions after the fire. The research area was dominated by tree species Cratoxylum glaucum, Acacia mangium, Melaleuca cajuputi, and understorey species dominated by Stenochlaena palustris. A diversity index value is classified as a medium for understorey and saplings and low for poles and trees.

PENGOLAHAN GULMA AIR MENJADI PUPUK ORGANIK DI KELURAHAN PALANGKA KECAMATAN JEKAN RAYA KOTA PALANGKA RAYA Jhon wesly manik; Vinsen willi Wardhana; Luqman Hakim; Rendy Muhammad Iqbal
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 8: Januari 2023
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v2i8.4524

Daerah Aliran Sungai (DAS) di jalan bukit keminting XV tumbuh tanaman gulma air yang membuat keindahan aliran sungai menjadi berkurang itu disebabkan kerna tanaman gulma air yang sangat cepat tumbuh dan tidak pengolahannya. Tujuan dari PKM ini adalah membantu masyarakat untuk mengolah tanaman gulma air yang berada di daerah aliran sungai untuk diolah menjadi pupuk organic bagi masyarakat. Metode yang digunakan dalam pelatihan ini adalah sosialisasi, pelatihan serta monitoring dan evaluasi. Pengetahuan masyarakat tentang tanaman gulma air menjadi berubah, sehingga masyarakat tahu cara pembuatan pupuk organic tanaman gulma yang dapat membantu pertanian masyarakat. Sehingga dengan kegiatan berkelanjutan daerah aliran sungai kembali bersih. Hasil yang didapat pada Kadar C-organik pupuk organik enceng gondok sebesar 18,93%, Kandungan pH pupuk organik sebesar 8,08, Kadar N total kompos enceng gondok sebesar 1,78%, Sedangkan nilai P total dan K total berturut-turut adalah 1,10% dan 1,26%. Nilai N+P+K yang dihasilkan sebesar 4,14%. Nilai tersebut lebih dari 4% yang berarti memenuhi persyaratan pupuk organik berdasarkan Peraturan Menteri Pertanian Nomor 70/Permentan/SR.140/10/2011 (Peraturan Menteri Pertanian Nomor 70/Permentan/SR.140/10/2011.

PELATIHAN KARAKTERISASI MORFOLOGI BAKTERI DAN FUNGI SEBAGAI PENGAYAAN PRAKTIKUM BIOLOGI BAGI GURU SEKOLAH MENENGAH ATAS Desimaria Panjaitan; Vinsen Willi Wardhana; Rizko Hadi; Faridah Tsuraya; Frans Grovy Naibaho
JMM (Jurnal Masyarakat Mandiri) Vol 7, No 1 (2023): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v7i1.12355

Abstrak: Salah satu kendala pelaksanaan praktikum di Sekolah Menengah Atas adalah rendahnya pengetahuan dan keterampilan guru yang seringkali sekaligus berperan sebagai pengelola laboratorium. Sebagai salah satu solusi terhadap kendala tersebut, maka dilakukan kegiatan pengabdian kepada masyarakat yaitu pelatihan karakterisasi morfologi bakteri dan fungi bagi guru Sekolah Menengah Atas di kota Palangka Raya. Kegiatan dilaksanakan pada bulan Oktober 2022 dan diikuti oleh 6 orang guru biologi sebagai mitra sasaran. Metode yang digunakan adalah pemaparan materi mengenai prinsip dan teknik karakterisasi morfologi bakteri dan fungi, diskusi, praktik, serta monitoring dan evaluasi kegiatan berdasarkan analisis kuisioner sebelum dan sesudah pelaksanaan pelatihan. Praktik mencakup pengamatan morfologi bakteri dengan teknik pewarnaan sederhana dan pewarnaan Gram sedangkan morfologi fungi diamati dengan teknik pembuatan preparat segar. Hasil pelatihan menunjukkan adanya peningkatan pengetahuan dan keterampilan mitra sasaran melakukan teknik karakterisasi morfologi bakteri dan fungi (dari 16,7% menjadi 100%). Selain itu, seluruh mitra sasaran tertarik (100%) mengimplementasikan kegiatan praktikum karakterisasi morfologi bakteri dan fungi bagi para peserta didik di sekolahnya masing-masing. Abstract: As an effort to increase teacher knowledge and skills, community service activities was carried out, namely training in the morphological characterization of bacteria and fungi for biology teacher from several senior high school in Palangka Raya city. The activity was held in October 2022 and was attended by 6 biology teachers. The method used were the presentation of material regarding the principles and morphological characterization techniques of bacteria and fungi, discussion, practice, monitoring and evaluation by analyzing the result of questionnaire before and after the training. Practice included observing the morphology of bacteria with simple staining techniques and Gram staining while the morphology of fungi was observed using techniques for making fresh preparations. The results of the training showed an increase in the knowledge and skills of the target partners in carrying out the morphological characterization techniques of bacteria and fungi (from 16,7% to 100%). In addition, all target partners were also were interested (100%) in implementing practicum activities for the morphological characterization of bacteria and fungi for students in their respective schools.

Exploration of pitcher plants in University of Palangka Raya Muhammad Rizki; Vinsen Willi Wardhana Wardhana; Mawardin Mawardin; Siti Sunariyati
BIOEDUSCIENCE Vol 5 No 2 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/526495

Background: This study is a pilot project on the effectiveness of flora, especially the type of semar bag (Nepenthes sp) found at the University of Palangka Raya (UPR). The research aims to identify semar bag plants at the University of Palangka Raya. The initial observations found at least more than one type of semar bag that lives in the peat forest of the UPR campus. The study was conducted in August-November 2020. Methods: Semar bag diversity data is collected by cruising methods. The data was analyzed using literature studies to be further identified using a sealed bag identification manual. Results: The study found three species of semar bags found in the forest campus of Palangka Raya University. The bag is a type of Nepenthes mirabilis (Lour.) Druce, Nepenthes gracilis Korth., and Nepenthes rafflesiana Jack. Morphological characters that distinguish these three species are the morphology of leaves and pouches. The range of environmental parameter values is air temperature 28-38oC, medium-open coverage, humidity 62-98%, and soil pH 5-7.5. Conclusions: This study's results are expected to be a database of flora biodiversity in Central Kalimantan.

Exploration of pitcher plants in University of Palangka Raya Muhammad Rizki; Vinsen Willi Wardhana Wardhana; Mawardin Mawardin; Siti Sunariyati
BIOEDUSCIENCE Vol 5 No 2 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/526495

Background: This study is a pilot project on the effectiveness of flora, especially the type of semar bag (Nepenthes sp) found at the University of Palangka Raya (UPR). The research aims to identify semar bag plants at the University of Palangka Raya. The initial observations found at least more than one type of semar bag that lives in the peat forest of the UPR campus. The study was conducted in August-November 2020. Methods: Semar bag diversity data is collected by cruising methods. The data was analyzed using literature studies to be further identified using a sealed bag identification manual. Results: The study found three species of semar bags found in the forest campus of Palangka Raya University. The bag is a type of Nepenthes mirabilis (Lour.) Druce, Nepenthes gracilis Korth., and Nepenthes rafflesiana Jack. Morphological characters that distinguish these three species are the morphology of leaves and pouches. The range of environmental parameter values is air temperature 28-38oC, medium-open coverage, humidity 62-98%, and soil pH 5-7.5. Conclusions: This study's results are expected to be a database of flora biodiversity in Central Kalimantan.

Prevalensi Escherichia coli pada Produk Asal Hewan serta Olahannya di Beberapa Swalayan Kota Bandar Lampung Ros Sari, Rakhmi; Zelpina, Engki; Wardhana, Vinsen Willi
JAS Vol. 9 No. 3 (2024): Journal of Animal Science (JAS) - Juli 2024
Publisher : Program Studi Peternakan, Fakultas Pertanian, Sains dan Kesehatan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kebutuhan protein dalam makanan sehari-hari sering dipenuhi melalui konsumsi produk peternakan, seperti daging merah, daging ayam, dan olahan lainnya. Pemerintah bertanggung jawab untuk memastikan keamanan dan kelayakan produk tersebut agar dapat dikonsumsi dengan nyaman oleh masyarakat. Penelitian ini bertujuan untuk mengevaluasi keberadaan E. coli pada produk asal hewan dan olahannya di swalayan Kota Bandar Lampung. Dengan menggunakan metode purposive sampling sebanyak 20 sampel daging sapi, daging ayam, dan produk olahannya diambil dari enam lokasi swalayan. Metode isolasi dan identifikasi mengacu pada SNI 2897:2008, dan hasilnya dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa dari 20 sampel, 40% positif E. coli. Prevalensi tertinggi terdapat di Super Market A dan B, masing-masing mencapai 100%, diikuti oleh Super Market F dengan prevalensi 66,66%. Temuan ini mengindikasikan adanya risiko kontaminasi E. coli pada produk asal hewan di swalayan Kota Bandar Lampung. Keberadaan E. coli dapat berasal dari proses produksi, pemrosesan, atau penanganan yang tidak higienis selama distribusi.

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