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All Journal Jurnal Medik Veteriner Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Made Damriyasa, Made
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Public Health Veterinary

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The Seroprevalence of Toxoplasma gondii in Cats at the House of Maternal Women with Toxoplasmosis in Badung, Indonesia Subrata, Made; Astawa, Nyoman Mantik; Suryadi, Nyoman Tigeh; Purnama, Sang Gede; Agustina, Kadek Karang; Harjana, Ngakan Putu Anom; Damriyasa, Made
Kesmas Vol. 16, No. 4
Publisher : UI Scholars Hub

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Abstract

Toxoplasmosis is a zoonotic disease caused by infection with the parasite called Toxoplasma gondii (T. gondii). The health and social impacts of the infectionare enormous, including miscarriage, hydrocephalus, blindness, and mental retardation. The occurrence of toxoplasmosis in maternal women cannot be se parated from cats around their houses. This study aimed to determine the seroprevalence of the parasite in cats found in the human carriers residences and identify the risk factors of toxoplasmosis in maternal women in Badung District, Bali Province, Indonesia. A total of 80 cat serum samples were obtained from two residential groups, 40 from the housing where the maternal women were infected and another 40 from where there were no identified sufferers of the disease. All the samples were examined using the enzyme-linked immunosorbent assay (ELISA) method to detect the presence of antibodies T. gondiiin feralcat serum. The results showed that 47.5% of the examined subjects had the said antibodies. As much as 65% came from housing with cases of toxoplasmosisin maternal mothers, and 30% came from residences with none. The presence of feral cats is a major risk factor for the transmission of T. gondiito humans.
Pathogenesis studies of African swine fever virus isolates from Bali and East Nusa Tenggara as a basis for inactivated vaccine development Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, Ketut; Sukada, Made; Mufa, Romy Muhammad Dary; Apsari, Ida Ayu Pasti; Dwinata, I Made; Ardana, Ida Bagus Komang; Damriyasa, Made; Sari, Tri Komala; Astawa, Nyoman Mantik; Supartika, Ketut Eli; Wirata, Ketut; Suarsana, Nyoman; Suartha, Nyoman
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.150-160

Abstract

African swine fever (ASF) is a highly contagious and lethal disease of pigs for which no effective vaccine is currently available. This study evaluated the pathogenicity of field African swine fever virus (ASFV) isolates from Bali and East Nusa Tenggara and assessed their potential for inactivated vaccine development using spleen-derived antigens. Based on the qPCR results, among all ASFV isolates tested, only the Bali isolate, designated B1, exhibited the highest DNA concentration, suggesting that this sample contained the highest ASFV titer. Consequently, the B1 isolate was selected and processed into chemically treated (CT-ASFV) and non-treated (NCT-ASFV) formulations. In the pathogenesis trials, pigs inoculated with NCT-ASFV developed acute ASF and died within 12 days, whereas those receiving CT-ASFV exhibited no clinical signs of ASF and showed no evidence of viral replication. In the vaccination trial, pigs immunized with CT-ASFV emulsified in Montanide™ ISA 50 V2 demonstrated survival in two out of three animals (67%) following challenge with a virulent strain, while all control animals succumbed to infection. Despite the absence of detectable humoral responses as determined by ELISA, the observed protection suggests a potential role for cell-mediated immunity. These findings indicate that the B1 isolate was highly virulent and represents a promising candidate for the development of an inactivated ASF vaccine. Further evaluation in larger-scale field trials is warranted.
Co-Authors Harjana, Ngakan Putu Anom I Made Dwinata I Made Subrata I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Wayan Masa Tenaya, I Wayan Masa Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Kadek Karang Agustina Ketut ELI Supartika KETUT SUADA Mufa, Romy Muhammad Dary Nyoman Suartha sang gede purnama Sukada, Made Suryadi, Nyoman Tigeh TRI KOMALA SARI Wirata, Ketut