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All Journal Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner Buletin Veteriner Udayana
Mufa, Romy Muhammad Dary
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health Veterinary

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Identification and Prevalence of Aspergillus sp Isolated from Bali Dog’s Skin Sudipa, Putu Henrywaesa; Puja, I Ketut; Dharmayuda, Anak Agung Gde Oka; Gunawan, I Wayan Nico Fajar; Sudimartini, Luh Made; Jayanti, Putu Devi; Sukernayasa, I Wayan; Mufa, Romy Muhammad Dary
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21594

Abstract

Aspergillus sp are fungi that commonly caused a systemic respiratory infection in Human and Dog. In Bali, most of the dog are free-roaming dogs, and has poor skin health condition. Some of them are suspected with fungal infection. Aspergillus sp has high risk to infect the dog, because they are available everywhere in environment. Therefore, this research is conducted for adding primary data of Aspergillus sp isolated from dog’s skin and their prevalence. The samples are 15 free-roaming Bali dogs with suspected fungal infection. Identification was carried out by using microscopic and macroscopic observation methods, skin swab was taken from the dog’s skin using the Mackenzie method, and the sterile toothbrush swab gently from the dog’s skin to the Sabouraud Dextrose Agar (SDA) media. The fungi were cultured on SDA media for macroscopic identification for approximately 3-4 days, mostly to observe how the fungal colony grew and its color. Microscopic identification are using adhesive tape, the tape was gently pressed into the fungal colony, the tape was stuck into the surface of object-glass with 3-4 drops of Methylene Blue staining, and the object glass was examined by using a light microscope, the findings of Aspergillus sp fungi are recorded and the data are descriptively presented. The result shows that most Aspergillus species that were found is Aspergillus fumigatus and Aspergillus niger according to their colonies growth and microscopic appearance, and the prevalence from 15 samples were 53.33%. Keywords: Aspergillus sp, fungi, Bali Dogs, Identification, Prevalence
PERCENTAGE OF ELIGIBILITY OF BALI CATTLE LIVER (BOS SONDAICUS) DURING THE 2025 EID AL-ADHA THROUGH POSTMORTEM EXAMINATION IN DANGIN PURI KELOD VILLAGE, DENPASAR CITY Irmawan, Adi; Mufa, Romy Muhammad Dary; Ardana, Ida Bagus Komang
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p23

Abstract

The celebration of Eid al-Adha as sacrificial worship is closely related to aspects of animal health and the safety of animal-derived food, as its implementation requires compliance with animal health and welfare principles, as well as assurance of meat suitability for public consumption. The increasing number of sacrificial animals each year necessitates greater attention to animal health to ensure food safety for the community. The liver of Bali cattle (Bos sondaicus) is an important indicator of the overall health of the body. This study aimed to determine the percentage of suitability of Bali cattle livers from sacrificial animals during Eid al-Adha through postmortem examination in Dangin Puri Kelod Village, East Denpasar District, Denpasar City, Indonesia. The study employed an observational method with a cross-sectional study design, involving 28 Bali cattle liver samples (total sampling). Examinations were conducted using inspection, palpation, and incision methods, with assessment parameters including color, shape, consistency, odor, and lesions. The results showed that 27 samples (96%) were normal and suitable for consumption, while one sample (4%) was abnormal and deemed unfit for consumption. These findings indicate that the majority of sacrificial Bali cattle livers in Dangin Puri Kelod Village met the ASUH standards (Safe, Healthy, Wholesome, and Halal). Continuous post-mortem examinations are necessary to ensure food safety and protect public health.
LEVEL OF KNOWLEDGE, ATTITUDE, AND PRACTICE OF GOOD MANUFACTURING PRACTICES (GMP) IN CHICKEN SLAUGHTERHOUSES AND CHICKEN CUTTING PLACES IN DENPASAR Anindya, Naura; Mufa, Romy Muhammad Dary; Tenaya, I Wayan Masa
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p20

Abstract

Chicken meat is the most widely consumed animal-based food commodity in Indonesia, making product quality and safety important. Chicken Slaughterhouses and Chicken Cutting Places serve as critical units in producing chicken carcasses that meet the principles of Safe, Healthy, Intact, and Halal (ASUH). This study aimed to determine the level of knowledge, attitudes, and practices regarding the implementation of Good Manufacturing Practice (GMP) in Chicken Slaughterhouses and Chicken Cutting Places in Denpasar City. This study used an observational design and a quantitative approach. Sampling was conducted using purposive and snowball sampling techniques. Fourteen respondents, including owners and quality control managers, were interviewed for this study. Data were collected through structured interviews using questionnaires. The level of GMP knowledge showed that 42.8% of respondents were in the good category, while the overall level of knowledge was low. The GMP attitude level showed that 85.7% of respondents had a positive attitude toward GMP implementation. Meanwhile, the GMP practice level indicated that 50% of the respondents were in the low category. GMP knowledge in Chicken Slaughterhouses and Chicken Cutting Places showed variation but tended to remain low. Although respondents’ attitudes toward GMP were generally positive, this was not yet reflected in their practices, which remained suboptimal and below the standard. This condition indicates a gap between knowledge and attitude and the actual implementation of GMP in the field. The recommendations of this study include continuous socialization and further research with more diverse respondents to improve knowledge, attitudes, and GMP practices among food handlers.
Pathogenesis studies of African swine fever virus isolates from Bali and East Nusa Tenggara as a basis for inactivated vaccine development Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, Ketut; Sukada, Made; Mufa, Romy Muhammad Dary; Apsari, Ida Ayu Pasti; Dwinata, I Made; Ardana, Ida Bagus Komang; Damriyasa, Made; Sari, Tri Komala; Astawa, Nyoman Mantik; Supartika, Ketut Eli; Wirata, Ketut; Suarsana, Nyoman; Suartha, Nyoman
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.150-160

Abstract

African swine fever (ASF) is a highly contagious and lethal disease of pigs for which no effective vaccine is currently available. This study evaluated the pathogenicity of field African swine fever virus (ASFV) isolates from Bali and East Nusa Tenggara and assessed their potential for inactivated vaccine development using spleen-derived antigens. Based on the qPCR results, among all ASFV isolates tested, only the Bali isolate, designated B1, exhibited the highest DNA concentration, suggesting that this sample contained the highest ASFV titer. Consequently, the B1 isolate was selected and processed into chemically treated (CT-ASFV) and non-treated (NCT-ASFV) formulations. In the pathogenesis trials, pigs inoculated with NCT-ASFV developed acute ASF and died within 12 days, whereas those receiving CT-ASFV exhibited no clinical signs of ASF and showed no evidence of viral replication. In the vaccination trial, pigs immunized with CT-ASFV emulsified in Montanide™ ISA 50 V2 demonstrated survival in two out of three animals (67%) following challenge with a virulent strain, while all control animals succumbed to infection. Despite the absence of detectable humoral responses as determined by ELISA, the observed protection suggests a potential role for cell-mediated immunity. These findings indicate that the B1 isolate was highly virulent and represents a promising candidate for the development of an inactivated ASF vaccine. Further evaluation in larger-scale field trials is warranted.
Co-Authors Anak Agung Gde Oka Dharmayuda Anindya, Naura I Ketut Puja I Made Dwinata I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Wayan Masa Tenaya, I Wayan Masa Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Irmawan, Adi Kadek Karang Agustina Ketut ELI Supartika KETUT SUADA Luh Made Sudimartini Made Damriyasa, Made Nyoman Suartha Putu Devi Jayanti Putu Henrywaesa Sudipa Sukada, Made Sukernayasa, I Wayan TRI KOMALA SARI Wirata, Ketut