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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Biotek Medisiana Indonesia Medical Journal of Indonesia Majalah Kedokteran Journal of Medicine and Health Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Cakradonya Dental Journal Journal of Clinical Microbiology and Infectious Diseases Journal of Medicine and Health
Mardiastuti H Wahid
Departemen Mikrobiologi Klinik, Fakultas Kedokteran, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Public Health

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Journal : Medical Journal of Indonesia

 1 
Patterns of bacterial resistance against Ceftriaxone from 2002 to 2005 in the Clinical Microbiology Laboratory of the Faculty of Medicine, University of Indonesia Rosana, Yeva; Kiranasari, Ariyani; Ningsih, Ika; Tjampakasari, Conny; Kadarsih, Retno; Wahid, Mardiastuti H.
Medical Journal of Indonesia Vol 16, No 1 (2007): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.758 KB) | DOI: 10.13181/mji.v16i1.248

Abstract

The spread of drug resistant microbes is a global public health challenge which impairs the efficacy of antimicrobial agents and causes substantial increase in morbidity and mortality rates, including healthcare-associated costs. Monitoring of antimicrobial drug resistance from documented microbial epidemiology & resistance rate is useful in preventing the emergence of resistance. This study reports on the pattern of bacterial resistance against ceftriaxone in the past 4 years. The data were obtained from specimens examined in the Clinical Microbiology Laboratory, Department of Microbiology Faculty of Medicine, University of Indonesia from 2002 to 2005. Microbial species were determined from culture and identification tests. Disc diffusion method was used for sensitivity testing of ceftriaxone to 14 Gram-negative and 7 Gram-positive bacteria. Although resistance rates were increased from 2002 to 2005, resistance rates of ceftriaxone were found to be less than 50%. Low resistance rates (< 3%) were observed for Salmonella typhi, Salmonella paratyphi A, Shigella flexneri, Serratia marcescens, and Streptococcus pneumoniae. These results could be useful in developing guidelines on the use of ceftriaxone in Indonesia. (Med J Indones 2007; 16:3-6) Keywords: Microbial drug resistance, disc diffusion method, Gram-positive, Gram-negative
Resistance patterns of microbes isolated from gastrointestinal tract Wahid, Mardiastuti H.; Ningsih, Ika; Adib, Azmier
Medical Journal of Indonesia Vol 20, No 2 (2011): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.388 KB) | DOI: 10.13181/mji.v20i2.437

Abstract

Background: Digestive tract infection with clinical manifestation of diarrhea is an infectious disease that has the highest morbidity and mortality rate, especially in developing countries. Diarrhea causes mortality mostly in infants under one year old. Improvement in management is done continuously, but advances in diagnosis and therapy cannot be reached by developing countries. One of the etiological agents causing infection of digestive tract is bacteria. Therefore, knowledge of bacteria that cause gastrointestinal infection and their resistance patterns may support the management of this disease. The aim of this study was to examine microbes that were isolated from the digestive tract and their resistance patterns against antibiotics.Methods: Samples (stool, rectal/anal swab) were collected from the Clinical Microbiology Laboratory, FKUI during 2005-2008. Isolation, identifi cation and sensitivity test were conducted according to standard laboratory procedures. Interpretation of sensitivity test was done according to NCCLS/CLSI guidance. Data was analyzed using WHOnet version 5.3.Results: We found 28 isolates of pathogenic Escherichia coli, 1 isolate of S. paratyphi A and 4 isolates of yeasts. Pathogenic Escherichia coli were still sensitive against some antibiotics, but the sensitivity was reduced against amoxicillin, sulbenicillin, ticarcillin and trimethoprim/sulfamethoxazole.Conclusion: The most predominant gastrointestinal tract infection causing microbes was pathogenic Escherichia coli. These bacteria showed decrease sensitivity against some antibiotics commonly used to treat patients with gastrointestinal tract infection. (Med J Indones 2011; 20:105-8)Keywords: antibiotics, Escherichia coli, gastrointestinal infection
Development of multiplex-PCR assay for rapid detection of Candida spp. Tarini, Ni Made A.; Wahid, Mardiastuti H.; Ibrahim, Fera; Yasmon, Andi; Djauzi, Samsuridjal
Medical Journal of Indonesia Vol 19, No 2 (2010): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.411 KB) | DOI: 10.13181/mji.v19i2.387

Abstract

Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identification of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp.Methods Five Candida spp. isolates were cultured, identifi ed with germ tube and API® 20 C AUX (BioMerieux® SA) kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay.Results DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of Candida spp. (Med J Indones 2010; 19:83-7)Keywords: Candida spp., multiplex-PCR
The susceptibility of pathogens associated with acne vulgaris to antibiotics Sitohang, Irma Bernadette Simbolon; Fathan, Hafizah; Effendi, Evita; Wahid, Mardiastuti
Medical Journal of Indonesia Vol 28, No 1 (2019): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.641 KB) | DOI: 10.13181/mji.v28i1.2735

Abstract

BACKGROUND Acne vulgaris is a pilosebaceous disorder. Bacterial activity and inflammation both influence acne formation. Antibiotics suppress the bacterial activities and elicit anti-inflammatory effects. The overuse of antibiotics may lead to resistance in bacteria. This study was aimed to provide an overview of bacteria that may cause acne and determine their susceptibility to antibiotics.METHODS This was a cross-sectional study sampling from 93 patients with acne in Ciptomangunkusumo Hospital. Comedones were extracted and cultured on Brucella blood agar, under aerobic and anaerobic conditions at 35oC for 24–48 hours. Bacterial identification was performed using Vitek®, and susceptibility test using E-test. Data interpretation was based on the Clinical and Laboratory Standards Institute 2015.RESULTS Staphylococcus epidermidis (50.5%), Propionibacterium acnes (11.0%), and Staphylococcus aureus (7.7%) were identified. Bacteria were not found in 69.2% and 1.1% of samples in anaerobic and aerobic cultures, respectively. P. acnes was susceptible to doxycycline (100%) and minocycline (100%), while 10% was resistant to erythromycin, clindamycin, and tetracycline. S. epidermidis was susceptible to minocycline (100%); but resistant to erythromycin (65.2%), clindamycin (52.2%) and tetracycline (32.6%). The susceptibility of S. epidermidis to doxycycline was 89.1%, which was lower than that of P. acnes (100%). S. aureus was found to be sensitive to minocycline (100%), doxycycline (71.4%), clindamycin (71.4%), and tetracycline (71.4%); but it was resistant to erythromycin (42.9%).CONCLUSIONS Doxycycline and minocycline showed 100% effectiveness for P. acnes. The isolated bacteria were more susceptible to doxycycline compared to tetracycline. The use of clindamycin and erythromycin needs to be limited as most S. epidermidis isolates were resistant to both.
Co-Authors Akbar, Resti Rahmadika Andi Yasmon Angga W Lokeswara, Angga W Ariyani Kiranasari Augustine Natasha Aziz, Mochamad Helmi Azmier Adib Azmier Adib Chairinda Dachwan Conny Tjampakasari Effendi, Evita Erie Yuwita Sari Fathan, Hafizah Fera Ibrahim Ibnu Agus Ariyanto Ika Ningsih Ika Ningsih Indah S. Widyahening Ingrid S. Surono Luthfi Saiful Arif Mochamad Helmi Aziz Mursinah Mursinah Mursinah, Mursinah Ni Made Adi Tarini Nurul Husna Oliver E Yausep, Oliver E Pratiwi Pujilestari Sudarmono Raksheeth Agarwal, Raksheeth Retno Asti Werdhani Retno Kadarsih Rimonta F Gunanegara Rimonta F Gunanegara, Rimonta F Rivia Gina Rahmawaty Samsuridjal Djauzi Sari, Meutia Kumala Sharon Hanmy Angel, Sharon Hanmy Sitohang, Irma Bernadette Simbolon Yeva Rosana