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Induced-Coagulated Plasma-Fibrin Gels as a Biological Scaffold for Cell Attachment and Proliferation of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC) Rio Hermantara; Fiano A. Kerans; Rizal R; E. Henny Herningtyas; Lutfan Lazuardi
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.872 KB) | DOI: 10.22146/ijbiotech.9310

Abstract

Fibrin gels are an ideal natural biological scaffold for tissue engineering because they are biocompatible,biodegradable, and have many biological surface markers. However, most research on fi brin gels used commercialfi brin kits that could be costly and limited in some areas. In this study, fi brin gels were made by inducing bloodcoagulation by adding a common diagnostic kit to assess the time for blood to clot, called activated partialthromboplastin time (aPTT). This induced coagulated plasma (iCoplas)-fi brin gels was evaluated for its ability toenhance biological activity of umbilical cord-derived mesenchymal stem cell (UC-MSC), which were cell attachmentand proliferation. Fibrinogen concentration had infl uence on cell attachment, where only 50% of the cells couldattach to 77 mg/dl fi brinogen gels whereas 93% cells adhered to 154 mg/dl fi brin gels. There were no signifi cantdifferences in cell proliferation on polysterene culture dish and fi brin gels (p>0.05). These results showed thatiCoplas-fi brin gels could be used as a fi brin-based scaffold, yielding no signifi cant difference than polysterene-tissueculture dish cultures in cell attachment and cell proliferation on 154 mg/dl fi brinogen concentration.
Pendampingan Gizi Seimbang pada Kader Posyandu Banjar Tengah Desa Blahbatuh Gianyar Bali Ni Putu Diah Witari; Fransiscus Fiano Anthony Kerans; Komang Trisna Sumadewi; Anak Agung Ayu Istri Prima Dewi; Ni Luh Putu Putri
Jurnal Peduli Masyarakat Vol 5 No 1 (2023): Jurnal Peduli Masyarakat: Maret 2023
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/jpm.v5i1.1497

Abstract

Gizi adalah salah satu aspek yang digunakan dalam mengukur tingkat kesehatan suatu negara. Pemantauan gizi pada balita sangat penting untuk mengetahui kondisi pertumbuhan Balita. Salah satu kegitan yang dilakukan oleh posyandu adalah pemantauan gizi. Kegiatan pemantauan gizi juga dilakukan oleh Mitra PKM kader posyandu banjar tengah, Blahbatuh Gianyar. Permsalahan yang dihadapi mitra adalah kurangnya pengetahuan mengenai gizi seimbang dan pemantauan gizi balita seta kekurang sarana dan prasarana posyandu. Sehingga fokus PKM meliputi pendampingan mengenai gizi meliputi pemahaman gizi, pengukuran pertumbuhan (Berat dan tinggi badan). Metode PKM meliputi dialog interaktif, pelatihan dan pendampingan pengukuran pertumbuhan serta evaluasi kegiatan. Hasil kegiatan didapatkan peningkatan pengetahuan mitra mengenai gizi (pengertian, jenis, pegolahan) sebanyak 40% dan keterampilan menggunakan pengukuran pertumbuhan terjadi peningkatan sebanyak 50%. Evaluasi kegiatan dilakukan 1 kali dalam sebulan pada setiap akhir kegiatan posyadu.
THE EFFECT OF NOISE EXPOSURE ON THE AVERAGE NUMBER OF TEMPORAL LOBUS NEURONS IN ADULT WISTAR RATS Suka Astini, Dewa Ayu Agung Alit; Evayanti, Luh Gde; Witari, Ni Putu Diah; Sumadewi, Komang Trisna; Prima Dewi, A.A.A Asri; Anthony Kerans, Fransiscus Fiano; Kurniawati, Ida
E-Jurnal Medika Udayana Vol 13 No 07 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i07.P09

Abstract

Noise is defined as unwanted and disruptive sound, considered an environmental stressor and disturbance. Noise encompasses many aspects of modern communities, including work environments. The damaging effects of noise primarily result from the unrestricted production of free radicals into the auditory organs. Exposure to noise causes various health problems, such as hearing impairment, sleep disturbances, and interference with individual performance. Reactive oxygen species (ROS), also known as free oxygen radicals, are normal by-products of cellular aerobic metabolism. These unstable molecules can damage cellular lipids, proteins, and nucleic acids in DNA if the antioxidant balance is disrupted. Acute and chronic exposure to loud noise generates excessive free radicals and disrupts extra-auditory organs such as the nervous, endocrine, and cardiovascular systems. The purpose of this study is to analyze the relationship between noise exposure and the number of neurons in the temporal lobes of Wistar rats. The research design used is a laboratory experimental design, specifically a pure experimental design, implemented with the randomized posttest-only control group design. The research sample consists of 30 male Wistar rats randomized into 2 groups, control and treatment. The treatment group is exposed to 95dB noise for 4 hours per day for 14 days. The rats are then euthanized, and their brain tissue is fixed with 10% neutral buffered formalin. Assessment is performed using Hematoxylin Eosin staining, and analysis is conducted blindly on the average number of neurons in the rat's temporal lobes. Data is subjected to normality testing using the Shapiro-Wilk test and analyzed using independent t-tests for parametric comparison. The results show a significant difference in the mean number of neurons in the temporal lobes between rats exposed to noise and the control group (p <0.005). The conclusion of this study is that noise affects neuron cells in the temporal lobes. Keywords : noise, temporal lobe, neurons.
EVALUATING THE EFFECTIVENESS OF ANATOMY AND HISTOLOGY PRACTICUM DURING COVID-19 PANDEMIC AT FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS WARMADEWA Evayanti, Luh Gde; Sumadewi, Komang Trisna; Witari, Ni Putu Diah; Suka Astini, Dewa Ayu Agung Alit; Kerans, Fransiscus Fiano Anthony; Dewi, A.A.Ayu Asri Prima; Ekayani, Ni Wayan Diana
E-Jurnal Medika Udayana Vol 13 No 6 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i06.P11

Abstract

The implementation of anatomy and histology practical sessions at Fakultas Kedokteran dan Ilmu Kesehatan (FKIK) Universitas Warmadewa during the Covid-19 pandemic still has many variations. This difference in implementation will have an influence on the achievement of student learning. This research aims to evaluate the knowledge of anatomy and histology among students by giving asynchronous learning, pre-test, post-test and practicum exam. This study using cross-sectional approach. 124 participants are 3th semester students whose active status at FKIK Universitas Warmadewa. The primary data were collected directly from practical sessions and practicum exam. The pre-test and post-test data were analyzed using paired-T test (p<0.05). The post-test and practicum exam were analyzed using Spearman rho (p<0.05). In anatomical practicum, there is an increase in post-test scores when compared to pre-test although not significant (p = 0.119). Anatomical practicum exam results have a meaningful positive correlation with post-tests (p = 0.013, r = 0.22). In histological practicum there was a significant increase in post-test scores when compared to pre-tests (p = 0.013), although practicum exam results were not significantly correlated with post-test (p = 0.410). Based on this study, improvements are needed related to technical of anatomical and histological practicums, as well as provisions related to practicums so that activities are structured
Pelatihan Pembuatan Mainan Edukasi Terhadap Peningkatan Pengetahuan Kader Posyandu dalam Stimulasi Perkembangan Balita Kurniawati, Ida; Witari, Ni Putu Diah; Dewi, A.A. Ayu Asri Prima; Kerans, Fransiscus Fiano Anthony; Wangsa, Putu Gde Hari; Adhitya, I Putu Gde Surya
International Journal of Community Service Learning Vol. 8 No. 2 (2024): May
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v8i2.76911

Abstract

Proses tumbuh kembang anak pada periode emas akan sangat menentukan fase tumbuh kembang anak selanjutnya. Deteksi dini dan stimulasi perkembangan anak perlu dilakukan secara berkala sebagai upaya untuk mengoptimalkan perkembangan dan mencegah masalah terkait yang dapat muncul pada anak. Penelitian ini bertujuan untuk memberikan pendampingan kepada mitra untuk meningkatkan keterampilan pembuatan mainan edukasi anak berbahan sederhana dan memberikan pengetahuan mengenai peranan mainan edukasi dalam stimulasi perkembangan anak. Penelitian ini merupakan penelitian deskriptif dengan menggunakan teknik pendekatan survey kepada masyarakat. Penelitian ini melibatkan subjek sebanyak 15 orang anak. Metode pelaksanaan PKM meliputi sosialisasi dan diskusi menggunakan Focus Group Discussion (FGD) untuk menentukan permasalahan di masyarakat.  Adapun instrumen yang digunakan adalah panduang FGD.  Setelah data dikumpulkan kemudian dianalisis menggunakan metode analisis deskriptif.  Hasil penelitian ini yaitu terdapat peningkatan keterampilan mitra dalam pembuatan mainan edukasi untuk stimulasi perkembangan anak. Sehingga dapat disimpulkan bahwa, pelatihan pembuatan mainan edukasi anak dapat meningkatkan kemampuan peserta untuk berkreasi dalam membuat mainan edukasi anak berbahan sederhana Penelitian ini memiliki dampak positif terhadap peningkatan pengetahuan bagi kader posyandu terkait pembuatan mainan edukatif untuk stimulasi tumbuh kembang balita. Permainan edukatif sangat bermanfaat dalam proses perkembangan anak.
Hematology Profiles in Colorectal Cancer Patients in Bali Kurniawati, Ida; Dewi, Anak Agung Ayu Asri Prima; Kerans, Fransiscus Fiano Anthony; Witari, Ni Putu Diah; Adyasputri, A A I Ayesa Febrinia; Sueta, Made Agus Dwianthara; Sumadewi, Komang Trisna; Evayanti, Luh Gde; Astini, Dewa Ayu Agung Alit Suka
Jurnal Ilmiah Kesehatan Vol 18 No 01 (2025): Jurnal Ilmiah Kesehatan (Journal of Health Science) 
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/jhs.v18i01.6936

Abstract

Colorectal cancer is a leading cause of cancer-related deaths worldwide. This study explores blood profile and cancer staging in colorectal cancer patients in Bali. It was a descriptive-analytical study that collected demographic and clinical data from medical records of colorectal cancer patients at Prof. Dr. I.G.N.G. Ngoerah Hospital. The results of the study showed the basic characteristics and hematology profiles of 100 colorectal cancer patients. The average age of patients was 55.6 ± 11.4 years, with 54% of the total sample being male. The average body mass index (BMI) was 21.1 ± 3.6 kg/m², with an average height of 161.9 ± 6.1 cm and an average weight of 55.3 ± 10.0 kg. Laboratory tests revealed an average hemoglobin (Hb) level of 11.0 ± 3.6 g/dL, red blood cell count of 4.0 ± 0.9 million/μL, white blood cell count of 11.2 ± 7.3 thousand/μL, and platelet count of 302.8 ± 154.8 thousand/μL. The average hematocrit (HCT) level was 33.7 ± 6.6 L/L. Liver function parameters indicated average aspartate aminotransferase (AST/SGOT) levels of 45.6 ± 63.6 U/L and alanine aminotransferase (ALT/SGPT) levels of 24.6 ± 29.4 U/L. Renal function was described by an average blood urea nitrogen (BUN) level of 21.8 ± 21.0 mg/dL, urea levels of 21.9 ± 21.2 mg/dL, and creatinine levels of 2.3 ± 11.5 mg/dL. Our study highlights the importance of hematology profiles in colorectal cancer patients as part of cancer screening examination. Further study with a larger number of samples and more diverse populations is needed to analyze the relation between variables.
Peningkatan Peran OSIS Dalam Pencegahan Kanker Serviks Dan Vaksinasi HPV Pada Siswi SMP di Bali Lestari, Desak Putu Oki; Analysa, Analysa; Kerans, Fransiscus Anthony; Winata, I Gde Sastra; Riasa, I Nyoman Putu
INCOME: Indonesian Journal of Community Service and Engagement Vol 4 No 3 (2025)
Publisher : EDUPEDIA Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56855/income.v4i3.1496

Abstract

Kanker serviks merupakan jenis kanker tertinggi ke-2 di Bali dan Indonesia, dengan infeksi virus HPV high risk sebagai faktor risiko utama. Meskipun vaksinasi HPV efektif menurunkan insiden kanker serviks, implementasinya di Indonesia, khususnya Bali masih baru dimulai dan tingkat pengetahuan remaja tentang kanker serviks masih rendah. Kegiatan pengabdian kepada masyarakat (PKM) ini bertujuan untuk meningkatkan pengetahuan, sikap, dan perilaku siswi SMP, khususnya pengurus OSIS terkait pencegahan kanker serviks dan vaksinasi HPV, serta mendorong peran aktif OSIS sebagai agen edukasi di lingkungan sekolah. Program dilakukan melalui penyuluhan aktif, pelatihan, dan pendampingan kepada pengurus OSIS di tiga SMP di Bali. Evaluasi dilakukan menggunakan pre-test dan post-test untuk mengukur perubahan pengetahuan, sikap, dan perilaku, serta pemantauan capaian vaksinasi HPV. Terdapat peningkatan signifikan pada nilai pengetahuan (dari rata-rata 3,47 menjadi 8,74), sikap (4,12 menjadi 9,32), dan perilaku (3,54 menjadi 9,01) setelah intervensi (p < 0,05). Cakupan vaksinasi HPV mencapai lebih dari 95%. Sebanyak 75% siswa mampu melakukan edukasi di kelompok kecil, dan 25% mampu melakukan penyuluhan di kelas besar. Intervensi berbasis OSIS efektif meningkatkan pengetahuan, sikap, perilaku, dan cakupan vaksinasi HPV pada siswa SMP. Peran OSIS sebagai duta kesehatan perlu terus didukung untuk memperluas dampak edukasi pencegahan kanker serviks di lingkungan sekolah.
Molecular and Histological Changes in Airway Basal Stem Cells on Chronic Obstructive Pulmonary Disease Dewi, A. A. Ayu Asri Prima; Inayati, Rizqa; Sumadewi, Komang Trisna; Kerans, Fransiscus Fiano Anthony
Indonesian Journal of Multidisciplinary Science Vol. 2 No. 10 (2023): Indonesian Journal of Multidisciplinary Science
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/ijoms.v2i10.586

Abstract

Airway basal stem cell changes in COPD including hyperplasia, squamous cell metaplasia, epithel-mesenchymal transision, impairment of cillia and secretoric non mucosal cell differentiation, and junctional barrier integrity disturbances that lead to histological changes. There are also molecular changes such as disturbances in gene expresion, cytokines, inflamation factor and dysregulation of basal cell transcriptome in locus 19q13.2. This article is aimed to review airway basal cell changes in COPD. By understanding initial changes of airway basal stem cell in COPD, hopefully can help to develop stem cell therapy in COPD treatment. The research aims to explore molecular and histological changes in airway basal stem cells on chronic obstructive pulmonary disease. The researchers used literature review as the way to verify their arguments on the issue discussed by analyzing the results of scientific sources, especially from trusted sources such as Google Scholar and Science Direct. The study continues by stating that regenerative therapy using stem cells has started to evolve with the advancement of stem cell technology and is anticipated to improve and treat this illness. Only preclinical and phase 2 clinical trials have seen recent advancements in the use of stem cells for treating COPD.
The Differences of 10% Neutral Buffered Formalin, 4% Paraformaldehyde, and Bouin Solution in Newborn Rat Brain Luh Gde Evayanti; Ni Putu Diah Witari; Komang Trisna Sumadewi; A.A.Ayu Asri Prima Dewi; Fransiscus Fiano Anthony Kerans; Dewa Ayu Agung Alit Suka Astini
WMJ (Warmadewa Medical Journal) Vol 9 No 1 (2024): May 2024
Publisher : Warmadewa University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmj.9.1.9180.1-4

Abstract

Rats are widely used in the study of embryological development of the central nervous system and its congenital disorders. Therefore, tissue fixation is very important in obtaining a good picture of the central nervous system, especially in newborn rats. This study aims to determine the difference in fixation using a solution of 10% neutral buffered formalin, 4% paraformaldehyde, and Bouin solution in the brain tissue of newborn rats. This experimental study used a post-test only controlled-group design with 18 newborn rats aged zero day. After randomization, the pups were grouped into 3 treatment groups, namely: fixation with 10% neutral buffered formalin solution (P1, n = 6), and fixation with 4% paraformaldehyde solution (P2, n = 6), and fixation with Bouin solution (P3, n = 6). Assessment using Hematoxylin Eosin staining, and analyzed by blinding based on the histology of the cerebral tissue (cell nucleus staining, cytoplasmic staining, staining clarity, uniformity of staining). Five cerebrum fields were examined. Data analysis used Kruskall Wallis test and post hoc Mann Whitney test using SPSS. The result was a significant difference in the histology of the brain tissue of newborn rats with the fixation of a solution of 10% neutral buffered formalin, 4% paraformaldehyde and Bouin solution (p = 0.001). Brain tissue fixation using 10% neutral buffered formalin solution showed better quality of histological pictures compared to 4% solution of paraformaldehyde solution and Bouin solution (p <0.05).
PEMBINAAN KELOMPOK IBU PKK TERKAIT HIPERTENSI KEHAMILAN DAN PEMANFAATAN TANAMAN HERBAL DI DAUHWARU, JEMBRANA Luh Gde Evayanti; Ni Putu Diah Witari; Dewa Ayu Ratna Juwita; Komang Trisna Sumadewi; A.A.Ayu Asri Prima Dewi; Fransiscus Fiano Anthony Kerans; Dewa Ayu Agung Alit Suka Astini
Warmadewa Minesterium Medical Journal Vol. 3 No. 2 (2024): May 2024
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmmj.3.2.2024.143-146

Abstract

Pengetahuan masyarakat terkait adanya hipertensi kehamilan di Kelurahan Dauhwaru masih kurang, terutama komplikasi yang akan dihadapi ibu hamil. Faktor kurangnya pengetahuan ini dipengaruhi oleh sosialisasi yang kurang tentang pentingnya pencegahan maupun kontrol tekanan darah pada ibu-ibu yang hamil. Ada banyak metode untuk menurunkan tekanan darah termasuk pemanfaatan tanaman sayuran sebagai obat herbal, salah satunya adalah pemanfaatan tanaman herbal. Tanaman herbal adalah tanaman yang dapat dipelihara sebagai tanaman lahan sempit di halaman rumah dan dikonsumsi sebagai sayuran atau jus untuk kesehatan. Mitra pengabdian ini adalah kelompok ibu PKK di Kelurahan Dauh Waru. Tujuan pengabdian ini adalah meningkatkan pengetahuan ibu PKK terkait hipertensi kehamilan dan pemanfaatan tanaman herbal kepada ibu-ibu hamil yang ada di lingkungan kelurahan. Metode yang digunakan berupa pembinaan dan praktek langsung cara mengukur tekanan darah mandiri. Selain itu, mitra juga diajarkan cara memelihara tanaman herbal dan memanfaatkannya sebagai tanaman herbal untuk mengontrol tekanan darah tinggi. Mitra juga dievaluasi melakui pre-test dan post-test. Setelah dilaksanakan kegiatan ini, terdapat perbedaan antara post-test dengan nilai pre-test secara signifikan (p < 0,05). Data ini menunjukkan terdapat peningkatan pengetahuan ibu PKK setelah kegiatan.
Co-Authors A A I Ayesa Febrinia Adyasputri A. A. Ayu Asri Prima Dewi A.A.Ayu Asri Prima Dewi A.A.Made Semariyani Aanak Agung Ayu Asri Prima Dewi Adhitya, I Putu Gde Surya Agus Pramana Shakti Manik Artha Anak Agung Ayu Asri Prima Dewi Anak Agung Ayu Asri Prima Dewi Anak Agung Ayu Asri Prima Dewi Anak Agung Ayu Asri Prima Dewi Anak Agung Ayu Asri Prima Dewi Anak Agung Ayu Istri Prima Dewi Anak Agung Made Semariyani Analysa - Anny Eka Pratiwi Asri Prima Dewi AA Ayu Asri Prima Dewi, Anak Agung Ayu Astini, Dewa Ayu Agung Alit Suka Desak Putu Oki Lestari Dewa Ayu Agung Alit Suka Astini Dewa Ayu Agung Alit Suka Astini Dewa Ayu Agung Alit Suka Astini Dewa Ayu Agung Alit Suka Astini Dewa Ayu Agung Alit Suka Astini Dewa Ayu Agung Alit Suka Astini Dewa Ayu Agung Alit Suka Astini Dewa Ayu Agung Alit Suka Astini Dewa Ayu Ratna Juwita Dewi, A. A. Ayu Asri Prima Dewi, A.A. Ayu Asri Prima Dewi, A.A.Ayu Asri Prima Dewi, Anak Agung Ayu Asri Prima E. Henny Herningtyas Ekayani, Ni Wayan Diana Evayanti, Luh Gede Gede Agus Surya Pratama Gede Agus Surya Pratama I Gusti Agung Istri Mahaswari I Gusti Ayu Agung Wulan Cahyani I Kadek Adi Aryatama I Ketut Suwiyoga I Wayan Sudiarta Ida Ayu Diah Kencana Dewi Manuaba Suka Ida Ayu Laxmi Ananda Dewi Manuaba Ida Kurniawati Ida Kurniawati Ida Kurniawati Ida Kurniawati Ida Kurniawati, Ida Inayati, Rizqa Komang Trisna Sumadewi Komang Trisna Sumadewi Komang Trisna Sumadewi Komang Trisna Sumadewi Komang Trisna Sumadewi Komang Trisna Sumadewi Komang Trisna Sumadewi Komang Trisna Sumadewi Luh Gde Evayanti Luh Gde Evayanti Luh Gde Evayanti Luh Gde Evayanti Luh Gde Evayanti Luh Gde Evayanti Luh Gede Evayanti Luh Gede Evayanti Luh Gede Evayanti Luh Gede Evayanti Luh Gede Evayanti Luh Gede Evayanti Luh Gede Evayanti Lutfan Lazuardi Md Ayu Mirah Dewanti Ni Gusti Agung Ayu Wulan Ananda Pratiwi Ni Luh Putu Putri Ni Luh Putu Putri Setianingsih Ni Made Gita Kusuma Dewi Ni Made Meishanda Kusuma Divayanti Ni Putu Ayu Dian Maharani Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari Ni Putu Diah Witari, Ni Putu Ni Wayan Diana Ekayani Prima Dewi, A.A.A Asri Putu Diah Witari Putu Gde Hari Wangsa Putu Yudha Asteria Putri Riasa, I Nyoman Putu Rio Hermantara Rizal R Saktivi Harkitasari Saktivi Harkitasari, Saktivi Sri Ratna Dewi Sueta, Made Agus Dwianthara Suka Astini Dewa Ayu Agung Alit Suka Astini, Dewa Ayu Agung Alit Sumadewi, Komang Trisna Tanjung Subrata Trisnia, Putu Ayunda