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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN ARTERI : Jurnal Ilmu Kesehatan Prosiding University Research Colloquium
Auliya, Qurrotu A'yuni
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Humanities Computer Science & IT Electrical & Electronics Engineering Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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Increased levels of Follicle Stimulating Hormone (FSH) and sertoli cells count in wistar rats induced with paraquat after green bean sprout extract supplementation Auliya, Qurrotu A'yuni; Nasihun, Taufiqurrachman; Chodidjah, Chodidjah
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 13, No 1 (2022): June 2022
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1094.221 KB) | DOI: 10.30659/sainsmed.v13i1.7882

Abstract

Paraquat herbicide disrupts the hormone Follicle Stimulating Hormone (FSH), causes testicular damage and changes in spermatogenesis. This study aims to determine the effect of supplementation of green bean sprout extract (Vigna radiata L.) on FSH levels, Sertoli cell counts in male Wistar rats (Rattus norvegicus) induced by paraquat herbicide. This research uses true experimental with posttest only control group design. A total of 25 rats were divided into 5 groups: P0 was given standard feed, P1 was given paraquat 4 4 mg/g BW, P2 was given green bean sprout extract supplement (GBES) dose 21.6 4 mg/g BW and paraquat 4 mg/g BW P3 was given GBES dose 43.2 4 mg/g BW and paraquat is administered orally for 15 days. Measurement of FSH levels using ELISA, while Sertoli cells were observed using testicular histology preparate. Mann-Whitney test resulted significance of p<0.05, which showed that the administration of GBES influenced FSH levels and the number of Sertoli cells. GBES doses of 21.6 4 mg/g BW, 43.2 4 mg/g BW, and 86.4 4 mg/g BW were shown to increase FSH levels and Sertoli cell counts in paraquat-induced Wistar rats. GBES increased FSH levels and the number of Sertoli cells in paraquat-induced rats.
Pengaruh Pemberian Kopi Robusta (Coffea canephora) terhadap Penurunan Kadar Glukosa Darah pada Tikus Rattus norvegicus dengan Kondisi Diabetes Melitus Sofyanita, Eko Naning; Maulana, Mochamad Rizal; Auliya, Qurrotu A'yuni; Setyowatiningsih, Lilik; Swastyaputri, Adita Puspitasari
ARTERI : Jurnal Ilmu Kesehatan Vol 5 No 3 (2024): Mei
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v5i3.439

Abstract

Diabetes Mellitus (DM) leads to an increase in Reactive Oxygen Species (ROS), which disrupts the function of pancreatic β-cells. The dependency on medications often occurs, hence there is a need for natural remedies with antihyperglycemic properties, such as coffee. Coffee contains high levels of antioxidants like chlorogenic acid, flavonoids, and caffeine, which are exogenous antioxidants that can activate NRF-2 and increase the activity of the enzyme Superoxide Dismutase (SOD), thus acting as an antihyperglycemic agent. This study aims to determine the effect of robusta coffee on blood glucose levels in rats with DM conditions. This experimental study used a Randomized Control Pretest-Posttest Group Design. The sample in this study included 24 male Rattus norvegicus rats, aged 3 months, with a body weight of 230-250 grams, divided into 4 groups randomly after a one-week acclimation period. The DM condition was induced by administering streptozotocin at 40 mg/KgBW, followed by a pretest blood glucose level examination after 3 days of streptozotocin induction. The diabetic rats were then given robusta coffee once a day for a period of 14 days. On the 15th day, a posttest blood glucose level examination was conducted. The results showed that the control group (KN) had no significant difference (p=0.530), while the treatment groups KP (p=0.003), KK1 (p=0.013), and KK2 (p=0.000) showed significant differences (p<0.05). The conclusion of this study is that the administration of robusta coffee (Coffea canephora) can reduce blood glucose levels in rats with diabetes mellitus.
Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kencana Ungu (Ruellia Tuberosa L.) Terhadap Bakteri Staphylococcus Aureus Dengan Metode difusi Cakram Mundriyastutik, Yayuk; Auliya, Qurrotu A'yuni; Rufaida, Etina Elva
Prosiding University Research Colloquium Proceeding of The 16th University Research Colloquium 2022: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Pulau Jawa tumbuhan kencana ungu tumbuh dengan subur sebagai tanaman liar pada ketinggian mulai dari 150 m di atas permukaan laut. Kencana ungu mengandung flavonoid, tanin, alkaloid, dan saponin yang memiliki peran sebagai antibakteri, antiinflamasi, dan antihistamin. Kandungan flavonoid dan alkaloid dapat digunakan untuk antibakteri yaitu menghambat bakteri Staphylococcus aureus yang merupakan bakteri dari penyakit dermatitis dan mastitis. Tujuan penelitian adalah untuk mengetahui aktivitas antibakteri yang mampu menghambat bakteri Staphylococcus aureus. Metode yang digunakan dalam penelitian adalah metode difusi cakram, variable bebas dalam penelitian adalah konsentrasi ekstrak etanol daun kencana ungu 15%, 20% dan 25% dan variable terikatnya adalah aktivitas antibakteri terhadap bakteri staphylococcus aureus. Hasil zona hambat pertumbuhan bakteri staphylococcus aureus pada hari ke 1 dan ke 14 yang dihasilkan pada konsentrasi 15% hasilnya 1,5 cm dan 1,3 cm. Konsentrasi 20% pada hari ke 1 diperoleh 1,8 cm dan pada hari ke 14 diperoleh 1,9 cm. Konsentrasi 25% pada hari ke 1 diperolah 2,3 cm dan pada hari ke 14 diperoleh 2,2 cm. Kesimpulan dari penelitian ini adalah ekstrak daun kencana ungu yang dapat menghambat bakteri staphylococcus aureus dengan konsentrasi optimum 25%.
Co-Authors Chodidjah Chodidjah Eko Naning Sofyanita Lilik Setyowatiningsih Maulana, Mochamad Rizal Rufaida, Etina Elva Swastyaputri, Adita Puspitasari Taufiqurrachman Nasihun Yayuk Mundriyastutik