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BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS Savitri, Lisa; Kasimo, Elfred Rinaldo; Ihsan, Kharisul; Faizal, Imam Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10708

Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues.

PENGARUH PEMBERIAN KOMBINASI METFORMIN DENGAN EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) TERHADAP KADAR GLUKOSA DARAH MENCIT DIABETES Khotimah, Khusnul; Kadir, Mujtahid Bin Abd; Kasimo, Elfred Rinaldo; Sanjaya, Rochmad Kris
Jurnal Mahasiswa Kesehatan Vol. 2 No. 2 (2021): MARET 2021
Publisher : Universitas Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/jumakes.v2i2.1216

People with diabetes mellitus extremely increase every year. Management of diabetes mellitus disease requires appropriate therapy to prevent serious increasing and progressing the disease to be serious complication then the achievement of therapy goals. This study aims to determine the effect of giving a combination of metformin and red dragon fruit peel extract (Hylocereus polyrhizus) on blood glucose levels of diabetic mice.This type of research is an experimental study with a pre-test and post-test design with a control group. As the test animal, Swiss webster male white mice were used. They were grouped into 6 groups, each with 4 tails for each group. As the treatment group by giving of a combination of metformin and red dragon fruit peel extract with various doses of 75 mg/kg body weight for mice, 150 mg/kg body weight for mice, 300 mg/kg body weight for mice, positive control for metformin 65 mg/kg BW for mice, comparative control extracts red dragon fruit peel 150 mg/kg BW of mice and negative control 0.5% Na CMC was given orally once a day for 14 days. Data analysis used the One Way Anova method followed by the Last Significant Difference (LSD) method.The results showed that the percentage of the highest reduction in blood glucose levels was 81% in the combination group with an extract dose of 300 mg/kg BW of mice which was significantly different from the negative control group and the comparison control group.

Histopathological Evaluation of Stomach Protection by Peperomia pellucida L. in Mice with Gastroenteritis Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Juwita, Syntia Tanu; Prasetyawan, Fendy; Saristiana, Yuneka; Mebung, Konradus Klala; Amanda, Cornelia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.535-540

Gastroenteritis, a widespread condition characterized by inflammation of the stomach and intestines, poses significant health challenges globally. Conventional treatments primarily focus on symptomatic relief and do not address the underlying gastric mucosal damage. Peperomia pellucida L., a medicinal plant known for its anti-inflammatory and antioxidant properties, has been suggested to possess gastroprotective effects. This study aims to evaluate the histopathological effects of P. pellucida L. on gastric protection in a mouse gastroenteritis model. Male Swiss mice were divided into six groups, receiving different treatments, including the ethanol extract of P. pellucida at varying doses (100, 300, and 500 mg/kgBW), followed by induction of gastroenteritis with Escherichia coli. Histopathological analysis was conducted to observe tissue damage across the treatment groups, including necrosis and cell degeneration. The results revealed that P. pellucida L. exhibited significant gastroprotective effects, particularly at a dose of 500 mg/kgBW, reducing gastric mucosal necrosis and inflammation compared to the control groups. The plant’s secondary metabolites, including flavonoids, tannins, saponins, and alkaloids, contributed to the observed protective effects by enhancing mucus production, reducing gastric acidity, and promoting tissue repair. These findings suggest that P. pellucida L. could be an alternative treatment for gastroenteritis and related gastric conditions, offering a natural approach to managing gastric inflammation and mucosal protection.

Edukasi Standar Penggunaan Air untuk Pembuatan Media Mikrobiologi pada Pemeriksaan Sediaan Kosmetik di PT. Triszie Lab Indonesia Savitri, Lisa; Kasimo, Elfred Rinaldo; Prasetyawan, Fendy; Saristiana, Yuneka; Mebung, Konradus Klala; Amanda, Cornelia
Abdimas Galuh Vol 7, No 2 (2025): September 2025
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v7i2.18780

Air merupakan komponen krusial dalam pembuatan media mikrobiologi, khususnya untuk pengujian sediaan kosmetik. Penggunaan air yang tidak memenuhi standar dapat menyebabkan kontaminasi, menurunkan validitas hasil uji, serta menghambat pertumbuhan mikroorganisme target. Oleh karena itu, laboratorium mikrobiologi wajib menggunakan air yang sesuai standar internasional seperti United States Pharmacopeia (USP) dan European Pharmacopoeia (EP), dengan pengawasan parameter seperti TOC, konduktivitas, dan cemaran mikrobiologi. PT. Triszie Lab Indonesia menyadari pentingnya edukasi mengenai standar mutu air, namun masih terdapat kesenjangan pengetahuan di antara personel laboratorium. Kegiatan pengabdian kepada masyarakat ini bertujuan memberikan pelatihan dan pendampingan terkait standar penggunaan air laboratorium. Metode yang digunakan meliputi observasi awal, pelatihan interaktif, penyusunan SOP, serta evaluasi melalui pre-test dan post-test. Hasil kegiatan menunjukkan peningkatan pemahaman teknis sebesar 50%, peningkatan praktik higiene, serta penerapan SOP yang sesuai standar USP dan WHO. Kegiatan ini juga mendorong perubahan budaya kerja dan kesadaran mutu di lingkungan laboratorium. Produk luaran seperti buku saku dan modul pelatihan mendukung keberlanjutan edukasi internal. Secara keseluruhan, kegiatan ini berkontribusi nyata terhadap peningkatan mutu pengujian mikrobiologi dan kesiapan laboratorium dalam menghadapi audit serta sertifikasi.

ANALYSIS OF KIDNEY HISTOPATHOLOGY IN SEPSIS MODEL OF MICE (MUS MUSCULUS) WITH PREVENTIVE TREATMENT OF LEAF EXTRACT FROM 'DAUN KENTUT' PLANT (PAEDERIA FOETIDA L.) AGAINST ESCHERICHIA COLI INFECTION Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Sukmawati, Datin An Nisa; Juwita, Syntia Tanu; Kholis, Akbar Nur; Mosse1, Yosep
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2946

Sepsis is a critical health concern characterized by organ dysfunction due to uncontrolled host reactions to infections. The prevalence of microorganisms causing sepsis varies, with Gram-positive bacteria and fungal sepsis gaining significance. Sepsis often leads to acute kidney injury (AKI), and its association with sepsis is known as sepsis-associated AKI (SA-AKI). The "Daun Kentut" plant, known for its diverse bioactive compounds, has shown potential in combating inflammation and oxidative stress. In this study, histopathological changes in mouse kidneys induced by Escherichia coli were investigated. Hemorrhage was the primary change observed. Dehydration caused by E. coli infection potentially led to kidney injury through hormonal responses. The plant extract's anti-inflammatory potential, attributed to compounds like saponins and flavonoids, was also explored. The study emphasizes the need for understanding sepsis-related kidney damage and suggests possible preventive strategies using natural compounds.

THE EFFECT OF Paederia foetida L. EXTRACT ON LIVER WEIGHT OF MICE SEPSIS MODEL INFECTED WITH Escherichia coli Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8965

Sepsis is a critical medical condition characterized by a systemic immune response to in-fection, which can lead to severe organ dysfunction and mortality. Bacterial sepsis, par-ticularly caused by Escherichia coli, poses a significant risk due to its potential to harm tissues and organs, including the liver. The liver plays a central role in metabolic process-es and host defense during sepsis, making it a key organ of interest. This study aimed to investigate the effect of Paederia foetida leaf extract on liver weight in a mice sepsis mod-el. Mice were divided into six groups: normal control (N), negative control (K-) receiving distilled water, positive control (K+) receiving ciprofloxacin, treatment 1 (P1) receiving P. foetida extract at 100 mg/kg BW, treatment 2 (P2) receiving 300 mg/kg BW, and treat-ment 3 (P3) receiving 500 mg/kg BW. After 14 days of treatment, significant differences in liver weight were observed among the groups, with the highest mean and standard de-viation recorded in the P1 group (1.3750 ± 0.3932). Liver abnormalities, including swell-ing, lobular thickening, and weight increase, were identified, indicating the liver’s adap-tive response to toxic substances during sepsis. Interestingly, the normal control group exhibited higher liver weights compared to the treatment groups, possibly due to fatty substance accumulation within the liver tissues. These findings suggest that P. foetida ex-tract may influence liver weight changes in sepsis, potentially modulating metabolic and detoxification processes. Further studies focusing on histopathological and biochemical mechanisms are needed to clarify the therapeutic potential of P. foetida in managing liver dysfunction associated with sepsis.

BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS Savitri, Lisa; Kasimo, Elfred Rinaldo; Ihsan, Kharisul; Faizal, Imam Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10708

Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues.

Karakterisasi Ecoenzyme dari Limbah Buah Nenas Jenis Smooth Cayenne Kultivar Pasir Kelud (PK-1) sebagai Disinfektan dalam Pembersih Lantai Krissanjaya, Rochmad; Hermanto, Dhony; Ismillayli, Nurul; Savitri, Lisa; Muttaqin, Saiful; Kasimo, Elfred Rinaldo; Probojati, Rasyadan Taufiq; Anjarwati, Aprilia; Apriana, Dita
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 2 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i2.2880

Limbah nanas Smooth Cayenne PK-1 berpotensi sebagai bahan baku ecoenzyme disinfektan alami. Penelitian ini mengkarakterisasi ecoenzyme dari tiga bagian buah (daging, kulit, batang) hasil fermentasi 90 hari, lalu memformulasikannya menjadi pembersih lantai. Hasil karakterisasi menunjukkan ecoenzyme dari kulit memiliki aktivitas antibakteri tertinggi, didukung kandungan fenolik dan pH asam (3,2–3,5). Formula pembersih lantai optimum diperoleh dengan konsentrasi 20% ecoenzyme kulit nanas. Formula ini memenuhi standar SNI 06-4085-1996: pH 7,2, viskositas 1150 cP, stabilitas emulsi baik, dan stabil selama 30 hari penyimpanan. Produk menunjukkan aktivitas antibakteri signifikan terhadap E. coli (zona hambat 12,5 mm) serta efektif menghilangkan noda minyak (90,2%). Analisis regresi mengonfirmasi 20% sebagai titik optimum. Hal ini menunjukkan bahwa ecoenzyme dari kulit nanas PK-1 merupakan disinfektan alami yang efektif dan stabil untuk pembersih lantai ramah lingkungan, sekaligus mendukung pemanfaatan limbah pertanian bernilai tambah.

Pengenalan Metode Imunodiagnostik Dasar untuk Pencegahan Penyakit Menular di Peternakan Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Prasetyawan, Fendy; Saristiana, Yuneka; Amanda, Cornelia; Mebung, Konradus Klala
Abdimas Galuh Vol 8, No 1 (2026): Maret 2026
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v8i1.23354

Penyakit menular pada ternak masih menjadi salah satu kendala utama dalam usaha peternakan rakyat karena dapat menurunkan produktivitas dan menyebabkan kerugian ekonomi. Salah satu upaya pencegahan yang dapat dilakukan adalah deteksi dini penyakit melalui metode imunodiagnostik dasar. Namun, tingkat pemahaman peternak mengenai prinsip kerja dan pemanfaatan metode imunodiagnostik masih relatif rendah. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan pemahaman peternak mengenai pengenalan penyakit menular serta prinsip dasar metode imunodiagnostik sebagai langkah awal pencegahan penyakit di peternakan. Kegiatan dilaksanakan melalui penyuluhan interaktif, diskusi dua arah, serta demonstrasi penggunaan alat imunodiagnostik dasar berupa kit uji cepat. Sasaran kegiatan adalah peternak ternak rakyat di wilayah sasaran pengabdian. Evaluasi dilakukan melalui pengukuran tingkat pengetahuan peserta sebelum dan sesudah kegiatan serta observasi partisipasi aktif selama kegiatan berlangsung. Hasil kegiatan menunjukkan adanya peningkatan pemahaman peternak terkait jenis penyakit menular, prinsip reaksi antigen–antibodi, dan manfaat penggunaan metode imunodiagnostik dalam deteksi dini penyakit. Selain itu, peternak menunjukkan antusiasme dan partisipasi aktif selama proses penyuluhan dan demonstrasi. Kegiatan ini memberikan kontribusi positif dalam meningkatkan kesadaran peternak terhadap pentingnya pencegahan penyakit menular melalui pendekatan diagnostik sederhana yang aplikatif di tingkat peternakan rakyat.

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