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All Journal Biology, Medicine, & Natural Product Chemistry Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat BIO-CONS: Jurnal Biologi dan Konservasi Jurnal Kesehatan Metro Sai Wawai Jurnal Mahasiswa Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Elfred Rinaldo Kasimo, Elfred Rinaldo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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THE EFFECT OF Paederia foetida L. EXTRACT ON LIVER WEIGHT OF MICE SEPSIS MODEL INFECTED WITH Escherichia coli Savitri, Lisa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8965

Abstract

Sepsis is a critical medical condition characterized by a systemic immune response to in-fection, which can lead to severe organ dysfunction and mortality. Bacterial sepsis, par-ticularly caused by Escherichia coli, poses a significant risk due to its potential to harm tissues and organs, including the liver. The liver plays a central role in metabolic process-es and host defense during sepsis, making it a key organ of interest. This study aimed to investigate the effect of Paederia foetida leaf extract on liver weight in a mice sepsis mod-el. Mice were divided into six groups: normal control (N), negative control (K-) receiving distilled water, positive control (K+) receiving ciprofloxacin, treatment 1 (P1) receiving P. foetida extract at 100 mg/kg BW, treatment 2 (P2) receiving 300 mg/kg BW, and treat-ment 3 (P3) receiving 500 mg/kg BW. After 14 days of treatment, significant differences in liver weight were observed among the groups, with the highest mean and standard de-viation recorded in the P1 group (1.3750 ± 0.3932). Liver abnormalities, including swell-ing, lobular thickening, and weight increase, were identified, indicating the liver’s adap-tive response to toxic substances during sepsis. Interestingly, the normal control group exhibited higher liver weights compared to the treatment groups, possibly due to fatty substance accumulation within the liver tissues. These findings suggest that P. foetida ex-tract may influence liver weight changes in sepsis, potentially modulating metabolic and detoxification processes. Further studies focusing on histopathological and biochemical mechanisms are needed to clarify the therapeutic potential of P. foetida in managing liver dysfunction associated with sepsis.
BLOOD SMEAR EXAMINATION AND DIFFERENTIAL COUNT IN RATS WITH ESCHERICHIA COLI ESBL AND KLEBSIELLA PNEUMONIAE CARBAPENEMASE INFECTIONS Savitri, Lisa; Kasimo, Elfred Rinaldo; Ihsan, Kharisul; Faizal, Imam Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10708

Abstract

Sepsis is an irregular body response to severe infection, triggering uncontrolled inflammation that can lead to extensive tissue damage. It can progress to septic shock with multiple organ failure, resulting in death if left untreated. Laboratory examinations, such as leukocyte differential count in hematology, help understand the distribution pattern of white blood cells associated with health conditions. Laboratory research was conducted on mice injected with E. coli ESBL or K. pneumoniae carbapenemase. After 24 hours, observations were made on apoptosis in the spleen and liver of mice. Mouse blood was processed to count white blood cell types with a differential count. The results were analyzed to compare the control group with the bacterial infection groups of E. coli ESBL and K. pneumoniae carbapenemase. The research results indicate that the neutrophil count in the E. coli ESBL group is still within the normal range and lower (44.5±1.915%) compared to the K. pneumoniae carbapenemase group (55.75±8.342%). Similarly, the lymphocyte count in the E. coli ESBL group is within the normal range and lower (77.5±3.109%) compared to the K. pneumoniae carbapenemase group (91.25±7.588%). This highlights the crucial role of neutrophils and lymphocytes in responding to severe bacterial infections such as K. pneumoniae carbapenemase. Previous studies indicate neutrophilia and lymphocytopenia as markers of severe bacterial infections. Neutrophils are the primary defense against bacterial infections and can be rapidly recruited to the infection site, while specific infections can trigger prolonged neutrophil recruitment from hematopoietic tissues.
Study of Sperm Utilization in Female Drosophila melanogaster of bdp and btx Strains Savitri, Lisa; Ihsan, Kharisul; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1151-1157

Abstract

This study investigates the patterns of sperm utilization in female Drosophila melanogaster of the bdp and btx strains through sequential mating with multiple males. The research was conducted as a descriptive observational study without experimental manipulation. Females of each strain were crossed with four different males in a specific order, with a two-day interval between each mating. F1 offspring phenotypes were recorded daily over a six-day period following the final mating. Data were analyzed descriptively and supported by somatic chromosome reconstruction to identify the male origin of each offspring. The results indicate that sperm utilization in D. melanogaster females occurs in both random and non-random patterns. Non-random utilization was observed when offspring were produced exclusively from the first male, with no contribution from subsequent males. For example, in the female btx × male N cross, only N female and N male offspring were produced, indicating that sperm from the first male was preferentially used. In contrast, random sperm utilization was evident in later matings, where offspring from multiple males appeared, suggesting that previously stored sperm remained viable and were used alongside or instead of sperm from later matings. These findings highlight the complexity of post-mating sexual selection in D. melanogaster, particularly the role of female sperm storage and utilization mechanisms. The ability of females to either favor the sperm of a particular male or mix sperm from several males may provide evolutionary advantages in terms of genetic diversity and offspring fitness. This study contributes to a better understanding of reproductive strategies and sperm competition in insects, with potential implications for broader studies in evolutionary biology and genetics.
Karakterisasi Ecoenzyme dari Limbah Buah Nenas Jenis Smooth Cayenne Kultivar Pasir Kelud (PK-1) sebagai Disinfektan dalam Pembersih Lantai Krissanjaya, Rochmad; Hermanto, Dhony; Ismillayli, Nurul; Savitri, Lisa; Muttaqin, Saiful; Kasimo, Elfred Rinaldo; Probojati, Rasyadan Taufiq; Anjarwati, Aprilia; Apriana, Dita
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 2 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i2.2880

Abstract

Limbah nanas Smooth Cayenne PK-1 berpotensi sebagai bahan baku ecoenzyme disinfektan alami. Penelitian ini mengkarakterisasi ecoenzyme dari tiga bagian buah (daging, kulit, batang) hasil fermentasi 90 hari, lalu memformulasikannya menjadi pembersih lantai. Hasil karakterisasi menunjukkan ecoenzyme dari kulit memiliki aktivitas antibakteri tertinggi, didukung kandungan fenolik dan pH asam (3,2–3,5). Formula pembersih lantai optimum diperoleh dengan konsentrasi 20% ecoenzyme kulit nanas. Formula ini memenuhi standar SNI 06-4085-1996: pH 7,2, viskositas 1150 cP, stabilitas emulsi baik, dan stabil selama 30 hari penyimpanan. Produk menunjukkan aktivitas antibakteri signifikan terhadap E. coli (zona hambat 12,5 mm) serta efektif menghilangkan noda minyak (90,2%). Analisis regresi mengonfirmasi 20% sebagai titik optimum. Hal ini menunjukkan bahwa ecoenzyme dari kulit nanas PK-1 merupakan disinfektan alami yang efektif dan stabil untuk pembersih lantai ramah lingkungan, sekaligus mendukung pemanfaatan limbah pertanian bernilai tambah.
Laboratory Detection of Toenail Onychomycosis Using Malt Extract Agar in Students Savitri, Lisa; Ihsan, Kharisul; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1325-1330

Abstract

Onychomycosis is a common nail disorder caused by dermatophytes, non-dermatophyte molds, and yeasts. Despite being more prevalent in older adults, it is increasingly reported among younger populations, including university students, due to lifestyle factors and communal exposures. Rapid and accurate diagnosis is critical for effective management, but conventional methods remain limited in sensitivity. This study aimed to evaluate the prevalence and etiological profile of toenail onychomycosis among university students using potassium hydroxide (KOH) microscopy and Malt Extract Agar (MEA) culture. A total of 98 students with clinical signs of onychomycosis provided toenail specimens. Direct microscopy examination with 20% KOH was performed, followed by culture on MEA at 28–30 °C for up to 21 days. Fungal isolates were identified based on macroscopic and microscopic morphology. Statistical analysis assessed associations between clinical features and culture results. KOH microscopy detected fungal elements in 59.2% of specimens, while MEA culture yielded growth in 63.3%. When combined, the overall prevalence of confirmed onychomycosis was 71.4%. Trichophyton rubrum was the most frequent isolate (35.5% of positive cultures), followed by non-dermatophyte molds such as Aspergillus and Fusarium, and yeasts including Candida albicans and Candida parapsilosis. Nail thickening and discoloration were significantly associated with culture positivity (p < 0.05). Onychomycosis is prevalent among university students, with MEA culture providing superior diagnostic yield compared to KOH microscopy alone. The identification of non-dermatophyte molds and yeasts underscores the importance of comprehensive diagnostic strategies for guiding effective treatment. Future studies should combine culture and molecular methods to enhance detection accuracy and clarify the clinical significance of emerging pathogens.
Co-Authors Alimansur, Moh Amanda, Cornelia Anjarwati, Aprilia Antoro, Ester Lianawati Apriana, Dita Datin An Nisa Sukmawati Dhony Hermanto Faradita, Firda Fredenna Fendy Prasetyawan Fitri, Devi Nor Freitas, Maria Do Carmo Da Costa Hilmi, Mochamad Hanif Ihsan, Kharisul Juwita, Syntia Tanu Kadir, Mujtahid Bin Abd Kholis, Akbar Nur khusnul khotimah Loviana, Novi Mebung, Konradus Klala Mosse1, Yosep Muslikha, Iza Dwi Muttaqin, Saiful Nurul Ismillayli Rasyadan Taufiq Probojati Retnowati, Ana Rochmad Krissanjaya Sanjaya, Rochmad Kris savitri, Lisa Wulansari, Ida Septika Yanti, Novirma Yuneka Saristiana