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All Journal Indonesian Journal of Urology Jurnal Biosains Pascasarjana Majalah Obstetri dan Ginekologi JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Folia Medica Indonesiana
Kartuti Debora, Kartuti
Department Of Medical Microbiology, Faculty Of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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COMPARISON OF POSITIVE BACTERIAL CULTURE RATE FROM URINE SPECIMEN AND CATHETER SWAB IN INDWELLING CATHETER PATIENTS Yusuf, Marzuki; Alif, Sabilal; Soebadi, Doddy M; Debora, Kartuti; P, Widodo J
Indonesian Journal of Urology Vol 17 No 2 (2010)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v17i2.345

Abstract

Objective: The study aims to study the difference between urinary culture before and after indwelling catheter insertion and also the difference in positive bacterial culture rate between urine and catheter swab at the 7th and 14th days. Material & method: The subject of this study were patients who used indwelling catheters in urology outpatient department. The sample was allocated into two groups of 10 patients each, 7 and 14 days group. Sterile urinary culture was initially checked before catheter insertion. After 7 and 14 days of catheterisation respectively, urine and intraluminal catheter swab were performed upon removal. All samples were examined in Microbiology Department using McConkey and Nutrient agar (Mayo technique – T/T33). After 24 hours incubation, bacterial colonies were identified. Results: All urinary cultures obtained before the study were sterile, after 7 days catheter insertion two cultures (20%) remained negative and the remainder (80%) became positive. McNemar test result was 0,008 (p<0,05). In 14 days group after catheter insertion only one (10%) remained negative while 9 others were positive for bacteria. Mcnemar test shows 0,004 (p<0,05). The urinary and catheter swab culture is not significantly different in 7 days of indwelling catheterization patients (0,500; p>0,05) and also in 14 days patients (1,000, p>0,005). While the catheter swab culture is significantly positive after administering the urinary catheter in 7 and 14 days of catheterization (0,002; p<0,05). Conclusion: There was significant difference in urinary culture positive rate before and after catheter insertion in 7th and 14th day. Bacteriuria rose sharply after urinary catheter insertion despite aseptic procedure. There was no difference in culture positive rate between urine and catheter swab at 7th day as well as 14th day.
Jahe mengurangi jumlah koloni uropathogenic Escherichia coli pada wanita menopause dengan infeksi saluran kemih asimtomatis Dony Rosmana; Gatut Hardianto; Kartuti Debora MS
Majalah Obstetri dan Ginekologi Vol. 24 No. 1 (2016): Januari - April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.852 KB) | DOI: 10.20473/mog.V24I12016.1-7

Abstract

Objective: To prove the effect of ginger in the UropathogenicEscerichia coli Colonies in menopausal women.Materials and Methods: A pra-experimental study with onegroup pre test-post test design. Ginger with a similar variety andage is turned into a powder capsule. The subject of the research ispatient in the geriatric and menopause outpatient clinicDr.Soetomo Central Hospital-Surabaya. Each subject taken gingerpowder for five days in row. Midstream clean catch urine wasperformed before and after the treatment in order to identify andcount the colony of Uropathogenic Escerichia coli.Results: 12 out of 52 participants had a positive UropathogenicEscerichia coli result from the culture before treatment. 5 subjectshad colony count 105cfu/ml and 7 subjects <10cfu/ml. 11subject shows a negative result and 1 subject still had a positiveresult although a number of colony is decrease cfu/mlbecome 2x103cfu/ml Tujuan: Membuktikan pengaruh jahe terhadap jumlah koloniuropathogenic Escerichia coli pada kultur urin dari wanitamenopause dengan infeksi saluran kemih asimtomatis.Bahan dan Metode: Penelitian pra-eksperimental one grouppretest-posttest design. Jahe dengan jenis dan usia panen samadiolah menjadi kapsul serbuk jahe. Subyek penelitian diperoleh dipoli Geriatri dan Menopause RSUD.Dr.Soetomo Surabaya. Setiapsubyek diberikan kapsul serbuk jahe selam 5 hari. Dilakukankultur urin tampung porsi tengah untuk identifikasi dan hitungjumlah koloni sebelum dan sesudah perlakuan.Hasil: Dari 52 partisipan, didapatkan 12 subyek dengan hasilidentifikasi dan hitung koloni kultur sebelum perlakuan yangpositif uropathogenic Escerichia coli, 5 subyek dengan hitungkoloni ≥ 105cfu/ml dan 7 subyek <105cfu/ml. Didapatkan 11subyek dengan hasil hitung koloni kultur ulangan steril dan 1subyek dengan hasil hitung koloni kultur ulangan tetap positifdengan jumlah koloni menurun 4cfu/ml menjadi 2x10cfu/ml3
Aktivitas Antibakteri dan Perubahan Morfologi dari Propionibacterium Acnes Setelah Pemberian Ekstrak Curcuma Xanthorrhiza Halimatus Zahrah; Arifa Mustika; Kartuti Debora
Jurnal Biosains Pascasarjana Vol. 20 No. 3 (2018): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.391 KB) | DOI: 10.20473/jbp.v20i3.2018.160-169

Abstract

AbstrakPenatalaksanaan utama pada masalah akne vulgaris adalah penggunaaan antibotik baik topikal maupun oral. Akan tetapi penggunaan antibiotik dinilai telah menimbulkan dugaan resistensi terhadap P. acnes sebagai agent penyebab akne sehingga mendorong berbagai pihak untuk mengembangkan preparat antiinflamasi yang dapat diberikan topical ataupun sistemik. Curcuma xanthorrhiza Roxb. memiliki senyawa utama xanthorrizol yang dinilai potensial untuk dikembangan sebagai antibakteri. Tujuan penelitian ini adalah untuk mengetahui kadar hambat minimum dan kadar bunuh minimum serta perubahan struktur dinding sel dari Curcuma xanthorrhiza Roxb. terhadap pertumbuhan Propionibacterium acnes. Desain penelitian yang di gunakan adalah eksperimen dengan sampel P. acnes berupa isolate stock culture (ATCC® 11827™) yang selanjutnya ditumbuhkan pada media MHA. Jumlah replikasi yang digunakan sebanyak 4 ulangan. Konsentrasi ekstrak Curcuma xanthorrhiza Roxb. masing-masing 6,25 µg/ml, 12,5 µg/ml, 25 µg/ml, 50 µg/ml dan 100 µg/ml. Pengukuran aktivitas antibakteri didasrkan pada KHM, KBM dan pengamatan struktur dinding sel bakteri melalui metode Microscop Electron Screening (MES). Pemberian ekstrak Curcuma xanthorrhiza Roxb. memiliki efek antibakteri terhadap bakteri P. acnes secara in vitro. Konsentrasi ekstrak 25 µg/ml merupakan kadar minimum yang mampu menghambat pertumbuhan P.acnes melalui dilusi cair, sedangkan konsentrasi minimal yang mampu membunuh P.acnes adalah 50 µg/ml. Bakteri P. acnes yang dipapar dengan ekstrak etanol Curcuma xanthorrhiza Roxb. mengalami perubahan morfologi berupa timbulnya dinding sel kasar kasar akibat penyusutan serta adanya dinding sel yang hancur sehingga sitoplasma keluar dan tampak seperti meleleh. Respon daya hambat pertumbuhan bakteri yang dihasilkan Curcuma xanthorrhiza Roxb. dipengaruhi oleh senyawa aktif yang terkandung didalamnya seperti minyak atsiri, alkaloid, flavonoid, tanin, kurkuminoid dan terpenoid. Kandungan xanthorrizol yang dimiliki mampu menghambat pertumbuhan P.acnes mampu merusak aktivitas enzim sel, selain itu kandungan Curcuminoid turut berperan dalam menghambat pertumbuhan bakteri dengan cara mendenaturasi dan merusak membran sel sehingga proses metabolisme sel terganggu  Kata Kunci: Antibakteri, Akne vulgaris, Curcuma xanthorriza Roxb., MES
In Vitro Antimicrobial Activity Evaluation of Ginger (Zingiber officinale) Absolute Ethanol Extract against Uropathogenic Escherichia coli (UPEC) Angeline Felicia; Kartuti Debora; Ramadhani Ramadhani
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 13 No. 2 (2022): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V13I22022.51-56

Abstract

Highlights: 1. Ginger (Zingiber officinale) has an antimicrobial activity against Uropathogenic Escherichia coli (UPEC) which commonly causes urinary tract infection (UTI) in women.2. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ginger against UPEC in this study was 1000 mg/ml. AbstractIntroduction: Uropathogenic Escherichia coli (UPEC) is commonly found in the urine culture of women with urinary tract infections (UTIs) and is often resistant to several antimicrobials. Herbals, such as ginger (Zingiber officinale), are known to have antimicrobial activity against various microorganisms. This study was conducted to determine the antimicrobial activity of ginger against UPEC.Methods: This was a true experimental study to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) using agar dilution method. The McFarland 0.5 suspension of UPEC was inoculated on agar with 6 different ginger concentrations, i.e. 2,000 mg/ml, 1,000 mg/ml, 500 mg/ml, 250 mg/ml, 125 mg/ml, and 62.5 mg/ml. The MIC and MBC were read as the lowest concentration without visible growth.Results: No visible growth of bacteria on agar at a concentration of 2,000 mg/ml and 1,000 mg/ml. Thus, the value of MIC and MBC for UPEC was 1,000 mg/ml.Conclusion: Ethanol extract of ginger has antimicrobial activity against UPEC. In this study, the MIC and MBC for UPEC was 1,000 mg/ml.
icaA/D Genes and Biofilm Formation of Methicillin-Resistant Staphylococcus aureus in Dr. Soetomo Hospital, Surabaya Suryanditha, Putu Arya; Rasita, Yoeke Dewi; Debora, Kartuti; Kuntaman, K
Folia Medica Indonesiana Vol. 54, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a global health concern. One of the factors causing hospital infection is related to the ability of MRSA bacteria to form biofilms. Polysaccharide intercellular adhesin (PIA), encoded by ica gene, have an important role in S. aureus intracellular accumulation and aggregation. The aims of this study was to analyze the relationship between icaA, icaD genes and biofilm production in MRSA carrier and clinical isolate in Dr. Soetomo Hospital Surabaya. This study was an observational study using cross sectional approach. The sample was 47 MRSA isolates is as follow 28 isolates from carrier and 19 were clinical isolates. All of MRSA isolates carried mecA gene. PCR was performed to detect icaA and icaD genes. Biofilm formation was detected using microtiter plate assay (MTP). icaA gene was detected in all isolates whereas icaD gene in 96,4% carrier isolates and all (100%) of clinical isolates. Positive MTP results showed in all (100%) of carrier isolates and 57,9% of clinical isolates. Statistic result was significantly different in biofilm formation between carrier and clinical MRSA isolates. The proportion of positive biofilm formation in isolate with positive icaA/D genes was 82.6%. There was not any association between icaA and icaD gene with biofilm production.
Comparison of Microbiological Examination by Test Tube and Congo Red Agar Methods to Detect Biofilm Production on Clinical Isolates Furtuna, Dewi Klarita; Debora, Kartuti; Warsito, Eddy Bagus
Folia Medica Indonesiana Vol. 54, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Biofilm on medical devices can cause significant diseases and deaths and give a large effecton disease transmission among patients and health providers and potentially increasethe cost of patient treatment. By knowing the presence of biofilm on a patient, one can differentiate the treatment management for that particular patient from the patients without biofilm on their medical device. The purpose of this study was to obtain diagnostic method to detect biofilm formation on isolates from the medical devices by simple method that is easy to do and can be applied in resource-limited microbiology laboratory. 36 specimens obtained from IV Line, CVC, urinary catheter and ETT were grown on Muller Hinton agar and continued with 3 methods, i.e., Test Tube method, Congo Red Agar method and Microtiter Plate Assay method. Results of this study showed Test Tube (nephelometer), Test Tube (visual) and Congo Red Agar in order to have the same sensitivity of 100% but has higher specificity compared to Test Tube method (visual) and Congo Red Agar method in detecting biofilm production on isolates from medical devices that had been plugged into patients body. The biofilm formation inside devices depends on factors, i.e., host, device and the microorganism itself.
Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA) Hidayah, Amaliyah Nurul; Wasito, Eddy Bagus; Debora, Kartuti; Basori, Achmad; Isnaeni, Isnaeni; Utomo, Budi
Folia Medica Indonesiana Vol. 55, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has been resistant to various types of antibiotics, so it is not easy to be treated with antibiotics and needs other solutions. Javanese citronella oil distilled from the Cymbopogon nardus plant is proven to function as an antibacterial agent (bacteriostatic and bactericidal), fungicide and repellent. This study aimed to prove that there is a positive correlation between bacteriostatic and bactericidal effects with antibiofilm and anticolony spreading from Javanese citronella oil on MRSA. The intended antibiofilm is a barrier to biofilm formation and eradication. Bacteriostatic and antibiofilm effects were tested using microtiter plates assay, bactericidal effect test with subculture into the media and anticolony spreading effect test with spot inoculation in Tryptic Soy Broth media supplemented with 0.24% agar. The bacteriostatic effect test data were analyzed using paired t-test, bactericidal effect using the Friedman test, antibiofilm effect test using Kruskall-Wallis and the results of all the tests correlated using Pearson and Spearman correlation. The statistical significance used was p<0.05. The results showed that Javanese citronella oil had a bacteriostatic concentration of 0.02% (v/v) and bactericidal concentration of 0.78% (v/v). The Pearson correlation test showed that there was a negative correlation between bacteriostatic and bactericidal effects on biofilm formation with r = -0.956 (p = 0.000), but the correlation was positive for biofilm eradication with r = 0.918 (p = 0.000) and anticolony spreading with r = 1.000 (p = 0.000).
Effect of Glucose Induction on Biofilm Density in Clinical Isolate Acinetobacter baumannii Patients in Intensive Care Unit of Dr. Soetomo Hospital, Surabaya Lindarto, Wira W; Wasito, Eddy Bagus; Debora, Kartuti
Folia Medica Indonesiana Vol. 56, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

This study aimed to analyze the effect of glucose induction on the clinical isolate biofilm density of Acinetobacter baumannii. Thirteen clinical isolates of A. baumannii non biofilm forming were collected from non-DM patients who were treated at the ICU of Dr. Soetomo Hospital, Surabaya, was treated with the addition of 0.08% glucose, 0.15% glucose, 0.2% glucose, and 0.4% glucose in TSB growth media, followed by biofilm density examination with Tissue Culture Plate Method (TCPM) using 96 wells flatbottomed polyesterene tissue culture plate and read by autoreader ELISA with a wavelength of 630 nm (OD630). Biofilm density obtained was analyzed using ANOVA statistical analysis. The results of OD630 showed that the biofilm density increased significantly at the addition of 0.2% and 0.4% glucose. There was a significant increase in biofilm density at the addition of 0.2% and 0.4% glucose so that the management of blood sugar levels in ICU patients was needed before and when medical devices were installed.
Proportions of Group B Streptococcus Isolation from Pregnant Women's Vaginal and Rectal Swab Specimens at a Tertiary Hospital in Surabaya, Indonesia , Ivanna; Wasito, Eddy Bagus; Debora, Kartuti
Folia Medica Indonesiana Vol. 59, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: • Rectal and vaginal swab specimens were collected from pregnant women, and there was no significant difference in the proportions of group B Streptococcus isolation. • Combined vaginal and rectal swab cultures provide a higher isolation of group B Streptococcus. Abstract : Group B Streptococcus is a Gram-positive bacterium found in women. It causes high-risk mortality in pregnant women, newborns, and the elderly. This study aimed to compare group B Streptococcus (GBS/Streptococcus agalactiae) proportions from different collection sites (vaginal and rectal swabs). This was an analytic observational study with a hospital-based cross-sectional design. A total of 74 swabs were taken from 37 pregnant women at 35–37 weeks of gestation. Each participant provided a vaginal swab and a rectal swab, which were cultured in Todd Hewitt broth, blood agar, and CHROMagar. The specimens were subsequently identified using the VITEK 2 system. The GBS isolation percentages from the vaginal and rectal swab specimens were determined to be 13.5% and 8.1%, respectively. The McNemar test had a result of 0.697, and the Cohen's kappa test had a result of 0.165. To conclude, there was no significant difference in GBS isolation proportions between the vaginal and rectal swab cultures. Combined vaginal and rectal swab cultures were required to increase GBS isolation from pregnant women.
Co-Authors , Ivanna Achmad Basori, Achmad Angeline Felicia Arifa Mustika Budi Utomo Dewi Klarita Furtuna Doddy M Soebadi Dony Rosmana Eddy Bagus Warsito Eddy Bagus Wasito Furtuna, Dewi Klarita Gatut Hardianto, Gatut Halimatus Zahrah Isnaeni Isnaeni Kuntaman Kuntaman Lindarto, Wira W Marzuki Yusuf, Marzuki Putu Arya Suryanditha Ramadhani Ramadhani Rasita, Yoeke Dewi Sabilal Alif Warsito, Eddy Bagus Widodo J P Yoeke Dewi Rasita