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Aktivitas Peredaman Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Nurhasanah, Devika; Nofran Putra Pratama; Sri Purwa Pujihastuti
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 1 No. 2 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i2.1223

Background: Antioxidants are compounds that prevent an oxidation reaction from free radical compounds and important role in capturing free radicals in the human body. Excessive free radicals in the body can cause tissue damage and degenerative diseases. Pandanus amarillyfolius are known to act as natural antioxidant that have the potential to capture free radical compounds, because of their presence of flavonoid compounds. Objective: This study aims to determine the free radical scavenging activity of DPPH against the leaf extract of Pandanus amaryllifolius. Method: Pandanus amaryllifolius extraction was carried out using the maceration method with methanol as solvent (1:10). Concentration was carried out using a rotary evaporator to obtain a thick extract. Phytochemical screening and TLC test were carried out qualitatively using the mobile phase n-butanol: acetic acid: water (6:2:2) and phytochemical testing including flavonoids, alkaloids, saponins, tannins, steroids or terpenoids to determine the presence of flavonoid compounds as antioxidants. Free radical scavenging activity was tested using DPPH free radical scavenger with concentrations of vitamin C 2, 4, 5, 6, 8 ppm and pandanus fragrance extract concentrations of 10, 15, 20, 25 ppm and measured using a UV-Vis spectrophotometer to calculate the IC50 value. Result: The results of the phytochemical screening test of fragrant pandan indicate the presence of flavonoid compounds. The Rf value of the TLC test obtained 0.765 quarcetin and 0.787 extract and the resulting yellow spots, the Rf value of pandan extract which was almost the same as the Rf value of quercetin indicated that the pandan extract may contain the same compounds as quercetin, namely flavonoid compounds. The results of the free radical scavenging activity of methanol extract of pandan leaves were 86,861 g/ml and for comparison, vitamin C was 39,103 g/ml. Based on the statistical analysis of the T-Test, it is known that there is a difference between the sample and the standard with a significant value (p <0.05), namely 0.001 and 0.023. Conclusion: The Pandanus amaryllifolius extract test using the DPPH method is in the strong category (IC50 <50 -100 g/ml) and the comparison of Vitamin C is in the very strong category (IC50 <50 g/ml).

Uji Aktivitas Antibakteri Infusa Kayu Secang (Caesalpinia Sappan L.) Terhadap Bakteri Escherichia coli ATCC 25922 dan Staphylococcus aureus ATCC 25923 Leswara, Dianita Febrina; Nurhasanah, Devika; Retno P., Maysi
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 2 (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i2.1460

Background: Escherichia coli and Staphylococcus aureus are two common types of bacteria that can cause infections in the human body. The treatment to overcome infections caused by Escherichia coli and Staphylococcus aureus bacteria is by administering antibiotics. The use of antibiotics that are not appropriate or not according to instructions can cause resistance so that alternative antibacterial agents from herbal plants begin to be developed. One of the plants known to contain antibacterial compounds is the secang plant. The secang wood stem is known to contain flavonoid and tannin antibacterial compounds. Objective: To determine the bacterial activity and Minimum Inhibitory Concentration (MIC) of secang wood infusa (Caesalpinia sappan L.) against Escherichia coli and Staphylococcus aureus. Method: Extraction of active compounds in secang wood was done by infundation method. The concentration of secang wood infusion that will be tested is 25%, 50%, 75% and 100%. Testing the antibacterial activity of secang wood infusion was carried out against Escherichia coli and Staphylococcus aureus using the well diffusion method. Results: Extraction of active compounds in secang wood is carried out using the infundation method. All concentrations of secang wood infusion tested 25%, 50%, 75% and 100%, showed antibacterial activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. The smallest concentration that showed inhibition of antibacterial activity was at a concentration of 25% with diameter of of inhibition zone is 20.52 mm in Escherichia coli ATCC 25922 and 20.58 mm in Staphylococcus aureus ATCC 25923 bacteria. Conclusion: Secang wood infusion has antibacterial activity with Minimum Inhibitory Concentration (MIC) at 25% concentration with strong inhibition

Potensi Antioksidan Ekstrak Metanol Fuli Pala (Myristica fragrans Houtt) Djamaluddin, Rosmiyati R; Putra Pratama, Nofran; Nurhasanah, Devika
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 1 (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i1.1517

Background : The mace is the part that surrounds the nutmeg seeds. Mace and nutmeg seeds are widely used as a spice in food products and in traditional medicine used as a remedy for stomach pain, analgesics, stimulants. Nutmeg mace also contains flavonoids and phenolics that have potential as antioxidants. Research Objective: Determine the antioxidant activity of methanol extract of nutmeg mace (Myristica fragrans Houtt) in reducing free radicals DPPH Research Method: Nutmeg macaques are extracted using methanol solvents through a maceration process (1:10), then the extracts obtained are tested, namely organoleptic tests, moisture content tests, phytochemical tests, KLT tests, and antioxidant tests using quercetin comparators with varying concentrations, namely 2 ppm, 4 pmm, 6 ppm, 8 ppm, and 10 ppm Research Results: The results of the organoleptis test showed that the methanol extract of nutmeg mace had a deep black color, a distinctive aroma, and a thick texture. 0.83% for moisture content test results. The results of phytochemical screening showed that nutmeg mace methanol extract was positive for flavonoids, phenolics, alkaloids, saponins, and tannins. The KLT test also showed positive results containing flavonoids. The free radical scavenging activity of DPPH from metanol extract of nutmeg mace showed an IC50 value of 13.781 ppm ±SD 0.247 and a standard IC50 value of 4.181 ppm ±SD 0.247 compared to quercetin. Based on statistical analysis, there was no significant difference because the data obtained was 0.074 > 0.05 Conclusion: Methanol extract of nutmeg mace has antioxidant activity in reducing DPPH free radicals and shows an IC50 value of 13.781 ±0,211 ppm which is included in the category of very strong.

Penentuan Kadar Fenolik Total Dan Peredaman Radikal Bebas Ekstrak Metanol Daun Bayam Hijau (Amaranthus hybridus L) Dengan DPPH Nurhasanah, Devika; Mafa , Sutri Hud; Pratama, Nofran
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 2, Special Edition (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i2, Special Edition.1477

Background: Free radicals are molecules that have one or more free or unpaired electrons, so that free radicals are unstable. Free radicals can be inhibited in the presence of antioxidant compounds. The compound antioxidant can be obtained from various sources, one of which is from the green spinach plant. Amaranthus hybridus L. is a herbaceous plant from the Amaranthaceae family that contains natural antioxidant compounds, namely phenolic compounds. Objective: This study aimed to determine the total phenolic levels contained in methanol extract of Amaranthus hybridus L. and what is the value of IC50 methanol extract of Amaranthus hybridus L. which is able to reduce DPPH free radicals. Method: Amaranthus hybridus L. were extracted with methanol solvent by maceration method in a ratio (1:10). Then a qualitative analysis was carried out in the form of phytochemical screening, identification of phenolic compounds by Thin Layer Chromatography (TLC) method using the methanol mobile phase: chloroform: n-hexane (1:9:1 v/v/v) and the stationary phase of silica gel GF254. Quantitative analysis of the total phenolic content test, and free radical suppression activity test by DPPH method which was measured using a UV-Vis spectrophotometer to calculate IC50. Result: Methanol extract of Amaranthus hybridus L. positively contains alkaloid compounds, phenolics, flavonoids, saponins, and tannins. The TLC Rf quercetin value is 0.475, and the Rf extract is two spots namely 0.35 and 0.837. Total phenolic content of 1.150 ± 0.025% and a free radical suppression activity of IC50 value of 14.786 ppm. Conclusion: Methanol extract of Amaranthus hybridus L. could be categorized as very strong.

The Effect of Extraction Time Variation on Caffeine Content in Robusta Coffee Extract using Ultrasound-Assisted Extraction Method Anggraini, Amelia Putri; Fildzah Arifah, Mitsalina; Sayyidah Yahya , Fina Ummu; Nurhasanah, Devika
Journal of Food and Pharmaceutical Sciences Vol 13, No 1 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.18750

West Lampung Robusta coffee has a higher caffeine content than other types of coffee. The variations in extraction time in ultrasound-assisted extraction (UAE) produced different caffeine contents than conventional extraction methods. This study aims to determine extraction time variation (15, 30, 45) influences caffeine contents in roasted coffee beans. The organoleptic test and alkaloid test evaluated Robusta coffee qualitatively. The extract obtained was measured by measuring the % MC, yield value, and wavelength scanning. Quantitative analysis using UV-Vis Spectrophotometry determined the caffeine content with different extraction times, and then each group was assessed using SPSS software. The coffee bean powder was dark brown, had a distinctive coffee aroma, bitter taste, and smooth texture, and contained alkaloids with the Mayer, Wagner, and Dragendorff tests. The yield and % water content of extract at 30 (36.938%; 6.39%) were higher than at 15 (24.111%; 6.07%) and 45 minutes (22.170%; 6.37%). Alkaloids of the extract were investigated at a wavelength of 273 nm. Caffeine contents were 0.977 ± 0.018 mgCE/mg at the 15, 0.792 ± 0.054 mgCE/mg at the 30, and 0.979 ± 0.026 mgCE/mg at the 45 minutes in significant (P<0.05). In conclusion, the difference in extraction time impacted the caffeine content of the robusta coffee bean.

The Effect of Extraction Time Variation on Caffeine Content in Robusta Coffee Extract using Ultrasound-Assisted Extraction Method Anggraini, Amelia Putri; Fildzah Arifah, Mitsalina; Sayyidah Yahya , Fina Ummu; Nurhasanah, Devika
Journal of Food and Pharmaceutical Sciences Vol 13, No 1 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.18750

West Lampung Robusta coffee has a higher caffeine content than other types of coffee. The variations in extraction time in ultrasound-assisted extraction (UAE) produced different caffeine contents than conventional extraction methods. This study aims to determine extraction time variation (15, 30, 45) influences caffeine contents in roasted coffee beans. The organoleptic test and alkaloid test evaluated Robusta coffee qualitatively. The extract obtained was measured by measuring the % MC, yield value, and wavelength scanning. Quantitative analysis using UV-Vis Spectrophotometry determined the caffeine content with different extraction times, and then each group was assessed using SPSS software. The coffee bean powder was dark brown, had a distinctive coffee aroma, bitter taste, and smooth texture, and contained alkaloids with the Mayer, Wagner, and Dragendorff tests. The yield and % water content of extract at 30 (36.938%; 6.39%) were higher than at 15 (24.111%; 6.07%) and 45 minutes (22.170%; 6.37%). Alkaloids of the extract were investigated at a wavelength of 273 nm. Caffeine contents were 0.977 ± 0.018 mgCE/mg at the 15, 0.792 ± 0.054 mgCE/mg at the 30, and 0.979 ± 0.026 mgCE/mg at the 45 minutes in significant (P<0.05). In conclusion, the difference in extraction time impacted the caffeine content of the robusta coffee bean.

The Effect of Extraction Method on Total Flavonoid Content of Hedyotis corymbosa L. Ulvia, Rengganis; Nurhasanah, Devika; Camelia, Azhar; Widianingrum, Lucia
Journal of Biotechnology and Natural Science Vol. 5 No. 1 (2025): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v5i1.13187

Hedyotis corymbosa L. (H. corymbosa) is an Indonesian herbal plant with many health benefits. This activity comes from secondary metabolite compounds, one of which is flavonoids. These compounds can be obtained through an extraction process, where the extraction method is one of the factors that can affect the levels of compounds. This study aims to determine the effect of conventional extraction methods: maceration and soxhletation with non-conventional methods, Ultrasound Assisted Extraction (UAE) on the total flavonoid content of H. corymbose. The sample was extracted using 70% ethanol solvent (1:10 w/v) with the maceration, soxhletation, and UAE methods. The total flavonoid content of the H. corymbosa extract was measured using UV-Vis spectrophotometry. The data obtained were then analyzed statistically using SPSS One-Way ANOVA, followed by Post Hoc Tukey with a 95% confidence level. The soxhletation method has the highest flavonoid content at 72.255±1.334 mg QE/g, followed by UAE at 69.118±1.782 mg QE/g, and maceration at 43.725±0.679 mg QE/g. Statistical analysis confirmed that the extraction method significantly influences total flavonoid content. While both soxhletation and UAE methods produced similarly high flavonoid contents, UAE offers a substantial advantage in efficiency due to its shorter extraction time, making it a promising alternative to conventional soxhletation.

DPPH Free Radical Scavenging Activity of Combined Leaf Extracts of Gynura procumbens (Lour.) Merr. and Psidium guajava L. Ulvia, Rengganis; Nurhasanah, Devika; Byhaqy, Azhari; Afrida, Virani Nur; Arifah, Mitsalina
MEDIA ILMU KESEHATAN Vol 14 No 3 (2025): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v14i3.1799

Background: The formation of free radicals in the body can be prevented by administering antioxidants. The leaves of Gynura procumbens (Lour.) Merr. and Psidium guajava L. individually possess antioxidant activity. The combination of the two plants is expected to produce enhanced antioxidant activity.Objective: This study aimed to determine the DPPH free radical scavenging activity of a combination of Gynura procumbens (Lour.) Merr. and Psidium guajava L. leaf extracts.Method: Sample extraction was performed using Ultrasound Assisted Extraction (UAE) with ethanol (1:10 w/v). Each extract was subjected to phytochemical screening and tested for DPPH free radical scavenging activity using UV-Vis spectrophotometry. An independent SPSS T-test analysis was performed on the antioxidant activity data (IC₅₀ values) with a 95% confidence level.Results: The results of the phytochemical screening test showed that each extract contains alkaloids, flavonoids, phenolics, tannins, and saponins. The DPPH free radical scavenging activity of the standard quercetin produced an IC₅₀ value of 2.231 ppm, and the combination of Gynura procumbens (Lour.) Merr. and Psidium guajava L. leaf extracts (1:1) produced an IC₅₀ value of 19.454 ppm.Conclusion: The combination of Gynura procumbens (Lour.) Merr. and Psidium guajava L. has very strong DPPH free radical scavenging activity.

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