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All Journal Medica Hospitalia Diponegoro International Medical Journal (DIMJ) JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Sigit Adi Prasetyo, Sigit Adi
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Dentistry Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health

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Antioxidant and Anti-inflammatory effect of Graptophyllum pictum (L.) Griff extract Study on SOD and COX-2 serum of experimental hemorrhoids Azhar, Abu; Riwanto, Ignatius; Nugroho, Eriawan Agung; Susilaningsih, Neni; Prajoko, Yan Wisnu; Budiono, Parish; Prasetyo, Sigit Adi
Medica Hospitalia : Journal of Clinical Medicine Vol. 7 No. 2 (2020): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.539 KB) | DOI: 10.36408/mhjcm.v7i2.515

Abstract

Background The 1st, 2nd and small 3rd degree hemorrhoids are non-operatively treated by using anti-inflammatory and plebotonic medicines, high fiber diet and changing lifestyle. Graptophyllum pictum (L.) Griff (GP) is potential to be a hemorrhoid medicine (anti-inflammatory and plebotonic medicine) since its anti-inflammatory effect may be related to antioxidant property. ObjectiveTo elaborate the anti-inflammatory and anti-oxidant effect of GP on experimental hemorrhoid in male Wistar rats. MethodsA Randomized Controlled Trial Post-test only design in hemorrhoid-like rats using croton oil induced into the anus for 30 seconds each day for 3 consecutive days. 14 Wistar rats were randomly allocated into 2 groups. Group 1 got normal saline solution, while group 2 treated with GPE 100mg/Kg bw for 5 consecutive days. On the 9th experimental day, the blood was drawn for Elisa examination of COX-2 and SOD levels. As COX-2 and SOD normally distributed, pool t-test and product moment correlation was used for statistical analysis. Results All of Wistar rats were still alive. COX-2 levels (ng/ml) in the GPE group was 266.28±158.47, was significantly lower compared to control (595.21±68.92) (p=0.000). Serum SOD levels (ng/ml) in the GPE was 77.00±11.0, and was significantly higher than the control 28.00±4.00 (p=0.02). Correlation between COX-2 and SOD serum level was significantly strong negative correlation (p=0,000 and r=-0.842) Conclusions GPE at a dose of 100 mg/kg bw has antioxidant and anti-inflammatory effects on artificial hemorrhoids through decreased COX-2 levels, and elevated SOD levels. There is a strong negative correlation between SOD and COX-2 serum level. KeywordsGraptophyllum pictum, hemorrhoids, Croton oil, COX-2, SOD.
Sleeve Gastrectomy Improved TGF-β and IL-10 Expression in Obesity and Diabetes Mellitus Rats Mukti, Sindhu Nugroho; Prasetyo, Sigit Adi; Shobirin, Muhammad Ali; Rozy, Abdul Mughni; Prajoko, Yan Wisnu; Sagap, Ismail Bin
Diponegoro International Medical Journal Vol 5, No 2 (2024): December 2024
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dimj.v5i2.24232

Abstract

Introduction: Atherosclerosis is a cardiovascular disease which is the leading cause of 16% of the world's total deaths. The main comorbidities of atherosclerosis are obesity and diabetes mellitus. One of obesity surgery treatment that is currently very often used is the Sleeve Gastrectomy. Sleeve Gastrectomy is the most effective and significant long-term therapy for weight loss and preventing various comorbidities such as heart disease. This study analyze the effect of sleeve gastrectomy on the expression of atherosclerosis anti-inflammatory cytokines (TGF-β and IL-10) in the abdominal aorta on the process of atherosclerosis in obesity and diabetes mellitus subjects.Methods: An in vivo laboratory experimental study with post-test control group design on 15 male rats (Sprague Dawley) that was randomly divided into 3 groups: K1 (negative control), K2 (positive control with obesity and diabetes mellitus) and P1 (obese and DM rats treated with sleeve gastrectomy). After 10th day of the procedure, we analyze the body weight, fasting blood glucose, and gene expression of TGF-β and IL-10 using the PCR method. Statistical analysis was done using Anova, Kruskal Wallis and Post Hoc LSD.Results: There was significant decrease in body weight (p=0,000) and fasting blood sugar levels (p=0,000) in the P1 compared to the K2. There was significant increase of TGF-β (p = 0,005) and IL-10 (p = 0,002) gene expression in P1 compared to K1 and K2.Conclusion: SG decreased body weight and fasting blood sugar levels of obese and DM subjects. Also improved the expression of atherosclerosis anti-inflammatory cytokines (TGF-β and IL-10) in abdominal aorta.
Exploring the effects of ursodeoxycholic acid-probiotic combination on Lactobacillus and liver enzyme levels for cholestasis management: An experimental study Nur, Muhammad Hasbi; Lestari, Endang Sri; Budiono, Bernadus Parish; Prasetyo, Sigit Adi; Muniroh, Muflihatul
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 1, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss1.art10

Abstract

Background: Cholestasis arises from compromised bile secretion due to hepatocyte dysfunction, leading to liver impairment. Available treatments show limited efficacy, with ursodeoxycholic acid (UDCA) being a primary option. Cholestatic conditions influence gut microbiota; therefore, probiotic therapy emerges as a potential approach. Objective: This investigation aimed to evaluate the impact of combined administration of probiotics and UDCA on Lactobacillus levels, as well as the levels of the enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in rats with restricted common bile ducts .Methods: A total of 35 male Sprague Dawley rats were randomly assigned to 7 groups, each comprising 5 members: K1 (healthy control), K2 (negative control with cholestasis), K3 (positive control with cholestasis given UDCA), K4 (cholestasis given 36 mg probiotics), K5 (cholestasis given 18 mg probiotics and UDCA), K6 (cholestasis given 36 mg probiotics and UDCA), and K7 (cholestasis given 54 mg probiotics and UDCA). The treatment duration was 21 days, during which blood samples were collected for AST and ALT analysis. Lactobacillus count was determined by quantitative polymerase chain reaction (PCR) analysis of fecal samples.Results: When UDCA and probiotics were given together in three different dosages, the Lactobacillus count significantly increased (p<0.05) compared to the other groups. Furthermore, compared to the other treatment groups, the UDCA-probiotic combination group exhibited noticeably lower AST and ALT values.Conclusion: Combining UDCA and probiotics elevated Lactobacillus count and decreased AST and ALT levels in cases of cholestasis more effectively than single therapy.
Exploring the remarkable effect of ursodeoxycholic acid and Allium sativum combination on MMP-9 and TIMP-1 levels in cholestatic rat’s model Sekartaji, Anindhita Dyah; Prasetyo, Sigit Adi; Muniroh, Muflihatul
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 3, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss3.art5

Abstract

Background: Cholestasis is a disturbance in the production or flow of bile that causes excessive accumulation of bile fluid and damage in the liver. Chronic liver damage will lead to liver fibrosis. Matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloprotease-1 (TIMP-1) play a critical role in liver fibrosis formation. Although ursodeoxycholic acid (UDCA) and Allium sativum extracts have long been renowned for improving liver function, their combination in alleviating liver fibrosis through the MMP-9 and TIMP-1 inhibitory pathways has yet to be studied.Objective: This study was conducted to assess the efficacy of UDCA and Allium sativum extract combination in altering MMP-9 and TIMP-1 levels, which are the main factors in the progression of liver fibrosis.Methods: We performed an experimental study with a post-test-only control group on 36 Sprague-Dawley rats, which were randomly grouped into healthy, negative, positive, and three treatment groups with UDCA (13.5 mg) and graded doses of Allium sativum extract (3.6 mg; 7.2 mg; and 14.4 mg). Cholestasis induction was done by a choledochal duct ligation, and treatment was given for 14 days. Levels of MMP-9 and TIMP-1 were analyzed after treatment.Results: Combining UDCA and Allium sativum improved the MMP-9 levels significantly in cholestatic rats, compared to administrating UDCA only (p <0.05). Combining UDCA and Allium sativum increased the TIMP-1 level (p < 0.05). Although the MMP-9 level results were under our existing hypothesis, TIMP-1 results showed surprising results. Matrix metalloproteinase-9 levels were strongly negatively correlated with TIMP-1 levels, with a r-value= 0.981.Conclusion: Combining Allium sativum and UDCA alleviates liver fibrosis progression through lowering levels of MMP-9 and increasing levels of TIMP-1.
Co-Authors Azhar, Abu Budiono, Bernadus Parish Endang Sri Lestari Eriawan Agung Nugroho, Eriawan Agung Ignatius Riwanto, Ignatius Muniroh, Muflihatul Neni Susilaningsih Nur, Muhammad Hasbi Parish Budiono Rozy, Abdul Mughni Sagap, Ismail Bin Sekartaji, Anindhita Dyah Shobirin, Muhammad Ali Sindhu Nugroho Mukti Yan Wisnu Prajoko