Claim Missing Document
Check
Articles

Found 37 Documents
Search

EKSTRAK DAUN KEJIBELING MENINGKATKAN FAGOSITOSIS DAN ROI MAKROFAG PADA MENCIT DIINFEKSI Staphylococcus aureus Setyawan, Annaas Budi; Lestari, Endang Sri; Winarto, Winarto
Jurnal Kesehatan Masyarakat Vol 11, No 2 (2016): JURNAL KESEHATAN MASYARAKAT (KEMAS) JANUARY 2016
Publisher : Jurusan Ilmu Kesehatan Masyarakat Fakultas Ilmu Keolahragaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v11i2.3712

Abstract

Penelitian ini bertujuan untuk membuktikan efek ekstrak daun kejibeling  terhadap peningkatan aktifitas fagositosis makrofag dan produksi ROI makrofag  pada mencit yang di infeksi bakteri S.aureus. Mencit dibagi menjadi empat kelompok. Kontrol (K), mencit diinfeksi bakteri S.aureus. Kelompok perlakuan (P1,P2,P3), mencit diinfeksi bakteri S.aureus kemudian diberi ekstrak daun kejibeling  dengan dosis bertingkat 150;300;600 mg/kg BB. Pemberian ekstrak daun kejibeling dilakukan selama delapan hari dan injeksi bakteri S.aureus dilakukan pada hari pertama dengan konsentrasi 108 cfu sebanyak 0,2 mL secara intraperitoneal. Penilaian aktifitas fagositosis makrofag dilakukan uji hipotesis Annova dilanjutkan analisis Post Hoc dengan uji LDS. Dan penilaian produksi ROI makrofag dilakukan uji hipotesis Kruskal wallis dilanjutkan analisis Post Hoc dengan uji Mann whitney U. Kedua uji hipotesis didapatkan p<0,05. Aktivitas fagositosis makrofag pada uji post hoc dengan uji LSD terdapat perbedaan bermakna kelompok kontrol (K) dengan perlakuan (P1,P2,P3) sedangkan kelompok perlakuan yang didapatkan perbedaan tidak bermakna yaitu; P1 dengan P3(p=0,150) dan P2 dengan P3(p=0,646). Produksi ROI makrofag pada uji Post Hoc dengan Mann Whitney menunjukkan perbedaan yang bermakna antar kelompok dengan nilai p<0,05. Ekstrak daun kejibeling dosis 150 mg/kg BB secara bermakna meningkatkan fagositosis makrofag dan produksi ROI.
EKSTRAK DAUN KEJIBELING MENINGKATKAN FAGOSITOSIS DAN ROI MAKROFAG PADA MENCIT DIINFEKSI Staphylococcus aureus Setyawan, Annaas Budi; Lestari, Endang Sri; Winarto, Winarto
KEMAS: Jurnal Kesehatan Masyarakat Vol 11, No 2 (2016)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v11i2.3712

Abstract

Penelitian ini bertujuan untuk membuktikan efek ekstrak daun kejibeling  terhadap peningkatan aktifitas fagositosis makrofag dan produksi ROI makrofag  pada mencit yang di infeksi bakteri S.aureus. Mencit dibagi menjadi empat kelompok. Kontrol (K), mencit diinfeksi bakteri S.aureus. Kelompok perlakuan (P1,P2,P3), mencit diinfeksi bakteri S.aureus kemudian diberi ekstrak daun kejibeling  dengan dosis bertingkat 150;300;600 mg/kg BB. Pemberian ekstrak daun kejibeling dilakukan selama delapan hari dan injeksi bakteri S.aureus dilakukan pada hari pertama dengan konsentrasi 108 cfu sebanyak 0,2 mL secara intraperitoneal. Penilaian aktifitas fagositosis makrofag dilakukan uji hipotesis Annova dilanjutkan analisis Post Hoc dengan uji LDS. Dan penilaian produksi ROI makrofag dilakukan uji hipotesis Kruskal wallis dilanjutkan analisis Post Hoc dengan uji Mann whitney U. Kedua uji hipotesis didapatkan p<0,05. Aktivitas fagositosis makrofag pada uji post hoc dengan uji LSD terdapat perbedaan bermakna kelompok kontrol (K) dengan perlakuan (P1,P2,P3) sedangkan kelompok perlakuan yang didapatkan perbedaan tidak bermakna yaitu; P1 dengan P3(p=0,150) dan P2 dengan P3(p=0,646). Produksi ROI makrofag pada uji Post Hoc dengan Mann Whitney menunjukkan perbedaan yang bermakna antar kelompok dengan nilai p<0,05. Ekstrak daun kejibeling dosis 150 mg/kg BB secara bermakna meningkatkan fagositosis makrofag dan produksi ROI.
TINGKAT KEPATUHAN PERAWAT RUMAH SAKIT X DI SEMARANG TERHADAP PELAKSANAAN CUCI TANGAN Caesario, Radya Irshadi; Wahjono, Hendro; Lestari, Endang Sri
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 8, No 2 (2019): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.16 KB) | DOI: 10.14710/dmj.v8i2.23834

Abstract

Latar Belakang : 1,4 juta pasien menderita infeksi nosokomial di negara berkembang maupun negara maju. Menjaga kebersihan tangan dengan baik dapat mencegah penularan mikroorganisme dan menurunkan frekuensi infeksi nosokomial. Perlu adanya penilaian terhadap angka kepatuhan mencuci tangan dalam meminimalisir infeksi tersebut. Tujuan : Mengetahui tingkat kepatuhan cuci tangan perawat Rumah Sakit X Metode : Penelitian ini merupakan penelitian obervasional deskriptif dengan desain cross-sectional. Ada 2 bentuk sampel pada penlitian ini, pertama, indikasi cuci tangan 5 momen. Kedua, perawat di ruang Lavender, Gladiol, Chrysant, dan ICU. Penelitian dilakukan selama periode September-Oktober 2017. Alat yang digunakan adalah lembar observasi indikasi 5 momen WHO dan kuisioner menilai karakteristik sampel. Hasil : Dari 365 indikasi 5 momen cuci tangan didapatkan tindakan cuci tangan sebanyak 138 (37.8%). Ruang dengan kepatuhan tertinggi adalah Lavender (77.7%). Seluruh responden telah mengetahui tentang 5 momen cuci tangan (71 responden). Sebanyak 60% responden telah bekerja selama kurun waktu 1-4 tahun. 85% responden telah mendapatkan pelatihan cuci tangan (42 responden). Lebih dari 90% responden menilai fasilitas dan lingkungan kerja kondusif. Kesimpulan : Tingkat kepatuhan cuci tangan 5 momen perawat RS X sebesar 37.7%.Kata Kunci : Kepatuhan, 5 momen cuci tangan.
ANTIMICROBIAL EFFECT OF ARROWROOT (Maranta arundinacea L.) METHANOLIC EXTRACT AGAINST Staphylococcus aureus BACTERIAL GROWTH Syahputra, Muhammad Ghaza; Antari, Arlita Leniseptaria; Winarto, Winarto; Lestari, Endang Sri
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 3 (2020): DIPONEGORO MEDICAL JOURNAL ( Jurnal Kedokteran Diponegoro )
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.863 KB) | DOI: 10.14710/dmj.v9i3.27499

Abstract

Introduction: Staphylococcus aureus is a spherical gram-positive bacterium that forms clusters and is frequently resistant to antibiotics. Methicillin-Resistant Staphylococcus aureus (MRSA) is a strain of S. aureus that is resistant to beta-lactam and cephalosporin groups of antibiotics, it is one of the biggest problems in resistant bacteria. Plants that have flavonoids in their extract can inhibit the growth of bacteria by impairing their cytoplasmic membrane. Arrowroot is an example of plants that have flavonoids in their extracts. Objectives: This research aims to determine the antimicrobial effect of arrowroot (Maranta arundinacea L.) methanolic extract against the growth of Staphylococcus aureus in vitro. Methods: This is true experimental research with a post-test control group design with two control groups and eight experimental groups. The sample of this research utilizes the MRSA strain of S. aureus received from the Microbiology Laboratory of Diponegoro University Faculty of Medicine that was eligible with the inclusion criteria. The research was conducted by using arrowroot extract concentrations 6.5%, 12.5%, 25%, 50%, and 100% to determine the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and inhibitory zone diameter. Results: MIC and MBC of arrowroot methanolic extract against MRSA is 100%. The arrowroot methanolic extract to have yielded an inhibitory zone diameter is 100% with a mean inhibitory zone diameter of 15.5mm. Conclusion: Arrowroot methanolic extract can inhibit the bacterial growth of MRSAKeywords: Staphylococcus aureus, growth,  M. arundinacea L. methanolic extract
THE INFLUENCE OF VARIOUS CONCENTRATED CHERRY (MUNTINGIA CALABURA) EXTRACT IN PREVENTING LACTOBACILLUS ACIDOPHILUS IN VITRO Nurhalisa, Siti; Lestari, Endang Sri; Wibisono, Gunawan; Indraswari, Darmawati Ayu
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 6 (2020): DIPONEGORO MEDICAL JOURNAL (Jurnal Kedokteran Diponegoro)
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v9i6.29326

Abstract

Background: Dental caries is a multifactorial illness caused by bacteria. Lactobacillus acidophilus is one of the bacteria causing caries, where it continues the caries process. That is why the antibacterial agent is needed. A cherry (Muntingia calabura) is fruit having many benefits for health, which one of them is as an antibacterial agent. It contains flavonoid, tannin, alkaloid, and terpenoid, which can function as the antibacterial agent.  Aim :The study aimed to determine the influence of cherry (Muntingia Calabura) extract in preventing the growth of L.acidophilus. Method: True experimental laboratory with a post-test only control group design and 24 samples. Cherries were extracted by using maceration. Then, the phytochemical test was conducted to discover antibacterial substances. The antibacterial test was done by using the dilution method to know Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: Minimum Inhibitory Concentration (MIC) could not be seen, and Minimum Bactericidal Concentration (MBC) showed that all concentration 6,25%, 12,5%, 25%, and 50% and positive control were able to kill a bacterium L. acidophilus. Conclusions: There is an influence of cherry extract on the grow
Changing the Function of Residential Space to Trade in Terms of The Occupant Aspect (Case Study Kambang Iwak Area Palembang City) Lestari, Endang Sri
International Conference on Multidisciplinary Research Vol 3, No 1 (2020): ICMR
Publisher : Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.186 KB) | DOI: 10.32672/pic-mr.v3i1.2598

Abstract

Increasing population growth in urban areas is generally accompanied by increasingly narrow job opportunities, causing many urban residents to carry out activities in the informal sectors, such as  small-scale trading activities. These activities are generally carried out by most residents living on theside of the main road. More than 50% of the residents’ houses on Jalan Merdeka Street of Kambang Iwak Palembang have changed their function to a commercial building; thus, it is necessary to conduct research on the trade public space that grows in the area. This research is field research that collectedvisual data and interviews with related parties. Research showed that the emergence of commercial public space in housing is caused by the proximity of the house and the relationship between the house and road space. The trading space functions as a public space, forming attributes in the form of accessibility, visibility, sociality, and adaptability. Commercial public spaces take place in the house and yard because they have proximity, visuality, adaptation, and activity related to public spaces. Keywords: Residential Space Function, Side of the Main Road, Merdeka Street of Kambang Iwak,
Pseudomonas aeruginosa Resistance in Southeast Asia Ciptaningtyas, Vincentia Rizke; Lestari, Endang Sri; Wahyono, Hendro
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 10, No 2 (2019): December 2019
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1026.072 KB) | DOI: 10.30659/sainsmed.v10i2.2986

Abstract

INTRODUCTION: The increasing number of multidrug-resistant Pseudomonas aeruginosa worldwide need to be continuously monitored to prevent its transmission. This study reviewed multidrug-resistant Pseudomonas aeruginosa in Southeast Asia.METHODS: Pubmed database was searched (January 2013-March 2018) for relevant articles using specific names of all eleven Southeast Asian countries. The articles retrieved were screened and analyzed to meet the inclusion criteria.RESULTS: A total of 17 articles met the inclusion criteria. Vietnam had the most published data included in this study, East Timor, Cambodia, and Laos had none. There are variations among Pseudomonas aeruginosa resistance in Southeast Asia. Thailand had complete data on antibiotic resistance and had the highest resistance rate of almost all antibiotics. Colistin had the lowest resistance rate among all antibiotics.CONCLUSIONS: Colistin had the lowest resistance rate among all antibiotics. Another study with more extensive data should be done to validate this study. Future research should be explicitly providing detail data results for antimicrobial resistance analyses.
Sterility of Gauze Packed in One and Three Layer Layer Parchment Paper Putri, Saskia Biyakto; Lestari, Endang Sri; Ciptaningtyas, Vincentia Rizke
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 1 (2017): June 2017
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.818 KB) | DOI: 10.30659/sainsmed.v8i1.1005

Abstract

Intoduction: Sterile gauze is one of the medical devices that are often used to prevent infection. Several things, one of them is materials for packaging, can affect the sterility of gauze. Parchment paper is one of the packaging materials for sterilization. The study was done to analyze the sterility of gauze packed with parchment paper one layer and three layers on the growth of microorganism.Method: This study was an experimental study with a quasi-experimental design. Samples were 60 gauze packed with one layer parchment paper and 60 gauze packed with three layers of parchment paper. The sterility of sterile gauze tested at weeks 0, 2 and 4. Results: There was no significant difference in the sterility of gauze packed with 1 layer of parchment paper on the growth of microorganisms at 0, 2, 4 weeks (p = 0.126). No significant difference in the sterility of sterile gauze packed with 3 layers of parchment paper at 0, 2, 4 weeks (p = 0.675). There was a significant difference in the sterility of sterile gauze packed with parchment paper 1 layer and 3 layers on the growth of microorganisms (p = 0.002).Conclusion There is a significant difference of sterile gauze packed with parchment paper 1 layer and 3 layers against the growth of microorganisms. The sterility of the packaged sterile gauze with a layer of parchment paper is better than the three layers.
COMPARISON OF THE WHO FORMULA A AND B HANDRUB EFFECTIVENESS AGAINST Staphylococcus aureus : STUDY IN DIPONEGORO NATIONAL HOSPITAL EMERGENCY ROOM, HIGH CARE UNIT, AND STOREROOM BASED ON prEN12054 MODIFICATION Daniel, Yohanes; Lestari, Endang Sri; Ciptaningtyas, V. Rizke
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 9, No 2 (2018): December 2018
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1939.667 KB) | DOI: 10.30659/sainsmed.v9i2.2639

Abstract

Introduction: Good implementation of hand hygiene and the availability of cheap, affordable, and effective hand rub can prevent healthcare-associated infection. WHO has issued hand rub formulations that are easily self-produced, effective, and affordable. Objective: This study compares the difference between handrub’s effectiveness stored in National Hospital Diponegoro University ER, HCU, and storeroom for 2 and 10 weeks.Methods: This study was an experimental study with pretest posttest randomized group design. The effectiveness of hand rub measured by prEN12054 in cfu/ml. Results: There was no significant difference between efficacy of WHO formula A and B (p=0.458). In the Mann-Whitney test there was no difference between the effectiveness of WHO A and B formulas before storage (p = 0.567), after being stored for two weeks (p=1.000), and ten weeks (p=0.539). In the Kruskal-Wallis test, there was no difference in the effectiveness of the WHO A formula in three sites at weeks 0, 2 and 10 (p = 0.275, 0.584, 0.116), there was no difference in the effectiveness of the WHO B formula in three sites at week 0, the 2nd and 10th (p = 0.289.; p = 0.584, p = 1.000).Conclusion: No significant differences were found on the effectiveness of the WHO A and B formulas. There was no significant difference in the effectiveness of WHO A or B formulas stored in three sites. There were no significant differences in the effectiveness of WHO A or B formulas, before and after being stored for two and ten weeks in three sites.
Model Pembelajaran Konstruktivis Metakognitif untuk Meningkatkan Kemampuan Berpikir Kritis Lestari, Endang Sri
Jurnal Basicedu Vol. 6 No. 2 (2022)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/basicedu.v6i2.2469

Abstract

Kemampuan berpikir kritis merupakan salah satu kemampuan yang dibutuhkan di tempat kerja. Selain itu, kemampuan berpikir kritis juga membantu dalam menyelesaikan masalah dan membangun strategi terbaik sehingga mampu membuat pekerjaan yang dilakukan menjadi lebih baik. Penelitian ini dilakukan dengan menggunakan metode Penelitian Tindakan Kelas (PTK) pada pokok bahasan sistem koordinasi dan alat indra. Subjek penelitian merupakan siswa kelas XI salah satu sekolah di kabupaten Karanganyar. Instrumen pengumpulan data menggunakan angket kemampuan berpikir kritis dan observasi lapangan, data kemudian dianalisis menggunakan teknik analisis deskriptif. Hasil penelitian menunjukkan bahwa terdapat peningkatan yang dignifikan pada tiap aspek kemampuan berpikir kritis peserta didik antara pra-dan pasca-siklus dengan rerata pra-siklus sebesar 32,46%, siklus 1 (50%), dan siklus 2 (78,17%). Hasil penelitian menunjukkan bahwa pemikiran kritis peserta didik dapat ditingkatkan dengan menggunakan model pembelajaran konstruktivis-metakognitif. 
Co-Authors Addin, Sofyan Rais Agung Nugroho, Eriawan Ahmad Syauqy Ahmad Zakaria Aisy, Jasmine Rikhdatul Ali, Raden Ahmad Nur Amelia Ayuningtyas, Amelia Anjani, Gemala Annaas Budi Setyawan Antari, Arlita Leniseptaria Asysyauki, Akhmad Hamdi Aynuddin, . Aynuddin, Aynuddin Bintang, Francisca Natalia Budiono, Bernadus Parish Caesario, Radya Irshadi Chairil Anwar Daniel, Yohanes Darmawati Ayu Indraswari Dewi Puspitasari, Vannya Diana Nur Afifah, Diana Nur Djanis, Ratnawati L. Djanis, Ratnawati Lilasari Djasmasari, Wittri Endang Wuryandini, Endang Eriawan Agung Nugroho, Eriawan Agung Fachrurrazie, . Fachrurrazie, Fachrurrazie Fadela, Dian Mira Fahmi Arifan Fatchur Rohman Gatot Jariono Gunawan Wibisono Haziman, Muhammad Luthfan Hendro Wahjono Hendro Wahyono Herawati Susilo Heryati, Agustina Kania, Adira Khamida Hikmawati Kis Djamiatun Lesi Hertati, Lesi Muniroh, Muflihatul Muttaqien, Rizal Arief, Nandhika, Wanda Mudya Neni Susilaningsih Ninik Rustanti Noor Pramono Nur, Muhammad Hasbi Nurdiani Nurdiani, Nurdiani Nurdiani, . Nurhalisa, Siti Nurhidayat Nurhidayat Nurkolis Nyoman, Ngurah Ayu Parmin - Pratama, Muhamad Bagas Wahyu Purnamasari, Evi Rachmi, Silvia Rachmy, Silvia Razak, Risdha Sagita Risa Dwita Hardianti, Risa Dwita Rosalina Rosalina Rosalina, . Samuel Agus Triyanto, Samuel Agus Sandra Eka Febrina Saputra, Tedy Setiawan Saskia Biyakto Putri, Saskia Biyakto Sastika, Anta Satria, Komang David Adi Sebastian Hadinata, Sebastian Sigit Adi Prasetyo, Sigit Adi Sinthika, Euodia Siti Susanti, Siti Styani, Erna Sudarmin Sudarmin Suherman SUTRIONO SUTRIONO Syahputra, Muhammad Ghaza Tambunan, Jenny Anna Margaretha Terttiaavini, Terttiaavini Titik Haryati V. Rizke Ciptaningtyas Wibowo, Gatot Winarto Winarto Yan Wisnu Prajoko Yora Nindita Zikri, Ridho Anzalla Zulfaidah Ariany