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All Journal KOVALEN: Jurnal Riset Kimia Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Analis Kesehatan Kendari
Nohong
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Physics

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Identifikasi dan Uji Aktivitas Antioksidan Senyawa Metabolit Sekunder Ekstrak Tanaman Cleome viscosa L: Identification and Antioxidants Activity Tests of Secondary Metabolite Compounds of Clome viscosa L. Plant Extracts Imran; Laily Nurliana; Muh. Natsir; Nohong; La Ode Abd Kadir; La Rudi; Ruslan; Thamrin Azis
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 1 (2023): April Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i1.16340

Abstract

Cleome viscosa L. has been used empirically by people on the island to treat diseases that have clinical symptoms such as malaria (fever, sweating, chills, and muscle aches). The purpose of this study was to determine the class of secondary metabolites and antioxidant activity. The extraction was carried out using the maceration method, while the fractionation was carried out using the separating funnel. The secondary metabolites found in the Cleome viscosa L. plant extract are alkaloids, flavonoids, steroids, terpenoids, tannins, and saponins. Antioxidant test conducted by DPPH method showed that the positive control of ascorbic acid and methanol extract obtained IC50 values of 3.86 ppm and 37.4 ppm, respectively.
UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI EKSTRAK KULIT BATANG PALIASA (Kleinhovia hospita Linn) Imran; Angga Febrian; Zainal Syam Arifin; Thamrin Azis; I Wayan Sutapa; Nohong; La Agusu; Syahrir; Alwahab
Jurnal Analis Kesehatan Kendari Vol. 7 No. 2 (2025): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 7 (2) Juni 2025)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v7i2.351

Abstract

This study aims to test the antioxidant activity of secondary metabolite compounds contained in paliasa (Kleinhovia hospita Linn) stem bark extract. This plant is traditionally known to have health benefits, but research on the antioxidant activity of its stem bark is still limited. Extraction was done using a maceration method using a methanol solvent, followed by liquid-liquid fractionation using n-hexane and ethyl acetate to separate compounds based on their polarity. Phytochemical screening showed that the n-hexane fraction contained alkaloids and terpenoids, while the ethyl acetate fraction contained alkaloids, flavonoids, tannins, and saponins. The antioxidant activity of both fractions was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method with absorbance measurements at a wavelength of 517 nm. The test results showed that the ethyl acetate fraction had an IC50 value of 51.7687 ppm, while the n-hexane fraction had an IC50 value of 60.5442 ppm. Based on the classification of antioxidant activity, both fractions are categorized as strong antioxidants (IC50 between 50-100 ppm). For comparison, vitamin C has an IC50 value of 14.3421 ppm, indicating strong antioxidant activity. The results of this study indicate that paliasa bark extract, especially the ethyl acetate fraction, has the potential as a source of natural antioxidants that can be further developed in the pharmaceutical, cosmetic, and food industries as an alternative to synthetic antioxidants with toxicity risks.
Isolation of Secondary Metabolite Compounds from the Soft Coral Isis sp., Antimicrobial Testing and Molecular Docking Studies Imran; Amalia, Fajri Fitra; Sahidin; Yodha, Agung Wibawa Mahatva; Nohong; Sutapa, I Wayan; Azis, Thamrin
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.10830

Abstract

Soft coral Isis sp. is a type of soft coral that contains many chemical compounds with various benefits that have not been widely studied. This study aims to identify and isolate secondary metabolite compounds contained in the ethyl acetate extract fraction of Isis sp. soft coral, determine of antimicrobial activity, and molecular docking study. Compound separation was carried out by maceration followed by fractionation using Liquid Vacuum Chromatography (LVC). The fractions obtained were identified by Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) technique. Four compounds were successfully identified through LC-MS/MS analysis namely Deoxyadenosine (C10H13N5O3), Spinasterol (C29H48O), Pseudoionone (C11H16O) and Isosalsoline (C11H15NO2). Antimicrobial activity test was conducted against Staphylococcus aureus, and Escherichia coli. The results of the antimicrobial activity test showed strong activity against S.aureus bacteria and E. coli bacteria with the same MIC value of 2 µg/mL. Based on molecular docking analysis can be predicted that compounds isolated from soft coral Isis sp., namely pseudoinone compounds have a role as anti-bacterial S. aureus (PDB: 6KVS). This compound also shows a strong ability as an anti- gram positive bacteria than gram negative. Isosalsoline compounds have a role as anti-bacterial E. coli (PDB: 5BNM). This compound also shows a strong ability as an anti-gram negative bacteria rather than gram positive.
Co-Authors Alwahab Alwahab Amalia, Fajri Fitra Angga Febrian I Wayan Sutapa Imran La Agusu La Ode Abd Kadir La Rudi Laily Nurliana Muh. Natsir Ruslan Sahidin Syahrir THAMRIN AZIS Yodha, Agung Wibawa Mahatva Zainal Syam Arifin