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All Journal Planta Tropika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Scripta Biologica BioEksakta : Jurnal Ilmiah Biologi Unsoed JURPIKAT (Jurnal Pengabdian Kepada Masyarakat)
Juni Safitri Muljowati
Faculty Of Biology, University Of Jenderal Soedirman, Purwokerto, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Veterinary

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Penggunaan Ekstrak Rimpang Lengkuas untuk Mengendalikan Busuk Leher Akar pada Tanaman Terong (Solanum Melongena L.) Juni Safitri Muljowati; Eddy Tri Sucianto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 2 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.2.243

Abstract

A research to find antifungal activity of galanga rhizome  (Alpinia galanga L.) on Sclerotium rolfsii  as the causal agent of white mold and stem rot disease on eggplant has been done.  The galanga extract was obtained by extracting the rhizome using ethanol solvent.  The experiment design was Completely Randomized Design (CRD) in a factorial pattern, involving two factors, i.e. the rhizome extract concentration (E) with six levels of 0%, 5%, 10%, 15%, 20%, and 25%; and application time (W) with two levels of  three days interval and seven days interval.  The result showed that the best extract concentration was 20% that was applied in three days interval.
Pemanfaatan Ekstrak Daun Nimba (Azadirachta indica A. Juss) untuk Pengendalian Penyakit Layu Fusarium pada Tanaman Tomat (Lycopersicum esculentum Mill.) Juni Safitri Muljowati; Aris Mumpuni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.275

Abstract

The aim of this study was to find out the effect of neem leaf extract (Azadirachta indica A. Juss.) on the growth medium of tomato plant toward fusarial wilt and to determine effective concentration and time of application of the extract to control the wilt. Experiments arranged in a Completely Randomized Design (CRD) was employed for in vitro antimicrobial assay and in a Randomized Complete Block Design (RCBD) for in planta antimicrobial assay, both of which were to find out the inhibitory effect of the extract on colony growth of Fusarium oxysporum (Schlecht). f.sp. lycopersici (Sacc.) Snyd. et Hans. (Fol).  The results indicated that neem leaf extract concentration of 5% applied at 7 DAP (Day After Planting) was the most effective treatment in inhibiting disease intensity of fusarial wilt on tomato plant showing inhibitory effect of up to 89.32%.
Pengaruh Kombinasi Jenis Bahan Pembawa dan Lama Masa Simpan yang Berbeda terhadap Produksi Pelet Biofungisida Trichoderma harzianum Juni Safitri Muljowati; Purnomowati Purnomowati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.188

Abstract

This research aimed to know effect of carrier material combination and different storage duration on viability of T. harzianum, also to know the material type and storage duration which yielding highest conidia.  Method used in this research was experimental by using Completely Randomized Design (CRD) with factorial pattern. The first factor was type of carrying material that were white sticky rice meal, mix of 75% white sticky rice meal and 25% mungbean meal, mix of 75% white sticky rice meal and 25% soybean meal, and mix of 75% white sticky rice meal and 25% skim milk that each of such material was inoculated by 108 conidia/ml of T. harzianum with storage duration levels that were 0 weeks, 3 weeks, 6 weeks, and 9 weeks.  Obtained data was analyzed by analysis of variance (F test) with significantly level 95% and 99% and followed by Honestly Significant Difference (HSD).  Result showed that there was interaction between types of carrying material with pellet storage period that showed significant effect on viability of T. harzianum.  Carrying material type of white sticky rice meal with pellet storage period of 9 weeks resulting highest percentage acerrage of conidia seedling (viability) of T. harzianum that was 78,19%.
Longevitas dan Efikasi Pelet Trichoderma harzianum terhadap Sclerotium rolfsii Penyebab Penyakit Layu pada Tanaman Tomat (Solanum lycopersicum L.) Juni Safitri Muljowati; Uki Dwiputranto; Iman Budisantoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.221

Abstract

Trichoderma harzianum is one of the antagonist fungi that can be used as a biofungicide to control soil-borne pathogens such as Sclerotium rolfsii, the cause of wilt isease of tomato. To ease the application of T.harzianum fungi in the field, it should be prepared a formulation in form of pellet. The longevity of T.harzianum pellet is determined by the viability of T.harzianum fungi contained in it, whereas the efication/ effectivity of T.harzianum pellet is determined by the longevity of the pellet.  The objectives of this study are to investigate the interaction between storage period and application dosages in controlling wilt disease of tomato;  and the dosage and storage period of T.harzianum on the effectiveness in controlling wilt disease of tomato. The experiment was carried out experimentally by using a Completely Randomized Design (CRD) in a factorial pattern. The first factors were storage period (W) consisted of 5 levels, i.e. 0, 3, 6, 9, and 12 weeks; the second factors were theapplication dosages (D) consisted of 6 levels, i.e. 0, 25, 50, 75, 100, and 125 grams. They were replicated 3 times each. The viability observation of T.harzianum was completed at the end of storage period of the pellets, the disease occurence and severity was since the 4th day after inoculation with 4 day interval until the tomato plant produced flowers. Research result showed that the interaction between storage period and different application dosages of T.harzianum pellets has influenced the increase of wilt disease control of tomato. Furthermore, storage period of 6 weeks and T.harzianum application of 50 g were the most effective in controlling wilt disease of tomato.
Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Cancer Cells Nuraeni Ekowati; Aris Mumpuni; Juni Safitri Muljowati
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7546

Abstract

Pleurotus ostreatus is a common mushroom cultivated in Indonesia, and potential properties of bioactive compounds for medicinal mushroom. This study was aimed at obtaining P.ostreatus extract bioactive compounds potential in inhibiting the proliferation of cervical cancer cells (HeLa) and evaluating the HeLa cell proliferation kinetics and HeLa cell death mechanisms. The research was beneficial in making this product can be easily applied in a more controlled industrial scale. Anticancer activity test through a cytotoxic test using the MTT [3- (4,5-dimetiltiazol-2-yl) -2.5-diphenyl tertrazolium bromide], the kinetics proliferation of HeLa cells and HeLa cell death mechanism was performed. Linear regression analysis was used to analyze the data. Ethyl acetate extract of P. ostreatus isolated from Madiun showed the best results with IC 50 = 107.59 µg / ml. HeLa cell proliferation kinetics analysis showed that the application of bioactive compounds 100 µg / ml resulted in an increase of in death of HeLa cells along with length of incubation time. An important finding was that HeLa cells death by apoptosis was greater than by necrosis.  In conclusion, the extracts of P. ostreatus  has the potential to inhibit the growth of HeLa cells. 
Pemberian Inokulum Fungi Mikoriza Arbuskula (Fma) Campuran terhadap Kemunculan Penyakit Busuk Pangkal Batang Sclerotium pada Tanaman Cabai Rawit dan Cabai Merah Pradana, Wira Dhyaksa; Dwiputranto, Uki; Muljowati, Juni Safitri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1817

Abstract

Cayenne pepper (Capsicum frutescens) and red chili (Capsicum annuum), including vegetables and fruit are widely consumed by the public, and also have many benefits. At present, the market demand for cayenne pepper and red chili is very high, so equalization must be made from the production sector. The conventional way of handling such as the administration of pesticides or other chemicals is less effective because it causes side effects that have a large enough impact, so an alternative technique is used that is to use Arbuscular Mycorrhizal Fungi (FMA) thus, research on the administration of Arbuscular Mycorrhoid Fungi Inoculum is thus carried out. (FMA) Mixture of Sclerotium Stem Rot Rotation in Capsicum frutescens and Capsicum annuum. The purpose of this study was to determine the effect of mixed FMA inoculums in suppressing the intensity of sclerotium stem rot rot disease in cayenne pepper (Capsicum frutescens) and red chili peppers (Capsicum annuum) and to determine the optimal dose of mixed AMF in suppressing the intensity of sclerotium stem rot rot on cayenne plants (Capsicum frutescens) and red chili plants (Capsicum annuum). This study used a Completely Randomized Design (CRD) with different doses of mixed AMF inoculums (0, 10, 15, 20, 25 g FMA with zeolite / plant carrier medium). The results of this study indicate that the treatment of mixed AMF inoculums on the intensity of sclerotium stem rot disease in cayenne and red chili plants can reduce the intensity of Sclerotium stem rot disease by 22% and in red chili plants by 11%.
Pengaruh Cercospora sp. terhadap Kandungan Asam Askorbat pada MekanismePatogenisitas Bercak Daun Tanaman Cabai : Kajian secara In vitro dan In planta Yuliawati, Nasriyatun; Mumpuni, Aris; Muljowati, Juni Safitri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1896

Abstract

Red chili is a vegetable commodity that has high economic value in Indonesia. Leaf spot disease caused by the fungus Cercospora sp. is one of the limiting factors in red chili production. The occurrence of leaf spot disease is determined by the success of the pathogenesis by the fungus Cercospora sp. In addition, red chilies that are resistant to leaf spot disease have higher ascorbic acid content than vulnerable red chilies. The purpose of this study was to determine the ability to grow pathogens Cercospora sp. on the medium which was given ascorbic acid and know the effect of inoculation of the pathogen Cercospora sp. against ascorbic acid content in red chili leaves (C. annuum L.). This study used an experimental design with a completely randomized design (CRD). In vitro tests carried out consisted of PDA and PDB medium which were given ascorbic acid with a concentration of 0 mg.l-1, 0,25 mg.l-1, 0,5 mg.l-1, 0,75 mg.l-1 and 1,0 mg.l-1. In planta testing was using hot chili red chili varieties, large red chili varieties and curly red chili varieties. The treatments that were tested included calculation of disease intensity and ascorbic acid content in red chili leaves. In vitro test the main parameters observed were the diameter colony of the fungus Cercospora sp. dan mycelium dry weight. In planta test the main parameters observed were the intensity of the disease, while the supporting parameters were the incubation period of the disease, the content of ascorbic acid in the red chili leaves, temperature and humidity. In vitro test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). In planta test data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, then the treatment that gave a real or very real difference was followed by the Least Significant Difference test (LSD). The results showed that the pathogen Cercospora sp. able to grow well on the PDA medium and GDP medium which were given ascorbic acid. Inoculation of pathogen Cercospora sp. can increase ascorbic acid content in red chili leaves.
PENGARUH Colletotrichum coccodes TERHADAP KANDUNGAN ASAM ASKORBAT PADA MEKANISME PATOGENISITAS ANTRAKNOSA TANAMAN CABAI: KAJIAN SECARA IN VITRO DAN IN PLANTA meilasari, fadhila; Muljowati, Juni Safitri; Mumpuni, Aris
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1918

Abstract

Patogen Colletotrichum coccodes merupakan salah satu patogen yang dapat menginfeksi tanaman cabai dan menyebabkan penyakit antraknosa terutama pada bagian buah dan daun. Tanaman dengan kandungan asam askorbat tinggi memiliki ketahanan yang lebih tinggi terhadap serangan patogen. Tanaman cabai yang tahan memiliki kandungan asam askorbat yang lebih tinggi dibandingkan dengan tanaman cabai toleran maupun rentan. Tujuan penelitian ini adalah mengetahui kemampuan tumbuh patogen C. coccodes pada medium yang diberi asam askorbat dan mengetahui pengaruh inokulasi patogen C. coccodes terhadap kandungan asam askorbat pada daun cabai. Penelitian dilakukan di Laboratorium Mikologi dan Fitopatologi, Laboratorium Lingkungan, & Greenhouse Fakultas Biologi Universitas Jenderal Soedirman, Purwokerto. Penelitian ini menggunakan dua uji yaitu uji in vitro dan uji in planta dengan metode eksperimental Rancangan Acak Lengkap (RAL), Uji in vitro menggunakan A) Medium PDA diberi asam askorbat; B) Medium PDB diberi asam askorbat dengan perlakuan penambahan asam askorbat sebanyak 0 mg.L-1 (kontrol); 0,25 mg.L-1; 0,50 mg.L-1; 0,75 mg.L-1; dan 1 mg.L-1, diulang sebanyak lima kali. Variabel bebas yang digunakan yaitu berbagai dosis asam askorbat, variabel terikatnya adalah pertumbuhan patogen C. coccodes. Parameter utama yaitu diameter koloni dan bobot kering miselium. Uji in planta menggunakan tiga varietas cabai (V1: Cabai merah hot chili; V2 Cabai merah keriting; V3: Cabai merah besar), uji A) Uji intensitas penyakit pada tanaman dan B) Uji kandungan asam askorbat. Masing-masing kelompok uji diulang sebanyak lima kali. Variabel bebas yang digunakan adalah varietas cabai merah, variabel terikatnya adalah nilai kerusakan tanaman berdasarkan kategori yang diamati pada waktu pengamatan yang ditentukan. Parameter utama yaitu intensitas penyakit, dan parameter pendukung yaitu periode masa inkubasi, kandungan asam askorbat pada daun cabai, temperatur, kelembaban dan pH tanah. Data uji in vitro dan uji in planta yang diperoleh dianalisis menggunakan analisis ragam (ANOVA) dengan tingkat kepercayaan 95%, dan perlakuan yang memberikan perbedaan nyata dilanjutkan dengan Uji Beda Nyata Jujur (BNJ) pada tingkat kepercayaan 95%. Hasil penelitian pada uji in vitro menujukkan bahwa patogen C. coccodes memiliki kemampuan tumbuh yang baik pada medium PDA dan medium PDB dengan penambahan asam askorbat. Hasil penelitian pada uji in planta, inokulasi patogen C. coccodes pada daun cabai merah dapat meningkatkan kandungan asam askorbat pada tanaman cabai merah Kata kunci : Colletotrichum coccodes, Cabai Merah, Antraknosa, Asam askorbat.
Histopathological Evaluation of Soybean (Glycine max (L.) Merr.) Strains Resistance to Sclerotium rolfsii Disease Samiyarsih, Siti; Erlina, Chindy Ayu; Muljowati, Juni Safitri; Fitrianto, Nur
PLANTA TROPIKA Vol 10, No 1 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i1.8907

Abstract

Sclerotinia infection of stem and leaf of soybean Glycine max (L.) Merr. caused by the fungal pathogen of Sclerotium rolfsii has recently become more important in the Indonesian soybean production area. This study aimed to evaluate the level of resistance and intensity of infection by S. rolfsii in four soybean strains. The research was arranged in a factorial completely randomized design. The observed variables include the anatomy characteristics of leaves and stems of soybean and disease intensity caused by S. rolfsii. The data were analyzed quantitatively with the Analysis of Variance (ANOVA) at 95% and 99% confidence level, followed by the Least Significant Difference Test (Fisher’s LSD) at the level of 5%. Soybean leaves and stem anatomy inoculated by S. rolfsii showed a decrease in the stomatal density, epidermis thickness, and mesophyll thickness as well as a damaged cuticle, damaged stem epidermis, and swollen stem cortex. Four strains inoculated by S. rolfsii showed a higher disease intensity of 40%-80% compared to the resistant cultivar ('Dering') and susceptible cultivar ('Wilis'), showing disease intensity of 20% and 40%, respectively.
Co-Authors Ade Winda Pradana Arif Rahman Hikam Aris Mumpuni Aris Mumpuni Eddy Tri Sucianto Endang Sri Purwati Erlina, Chindy Ayu fadhila meilasari Fitrianto, Nur Iman Budisantoso Juwarno, Juwarno Maman Maman meilasari, fadhila Nasriyatun Yuliawati Nuniek Ina Ratnaningtyas Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Oedjiono Pradana, Wira Dhyaksa Pratiwi, Ade Yuanita Putri Purnomowati Purnomowati Rochmatino Rochmatino Rubiati Fadhilah Siti Samiyarsih Siti Samiyarsih Uki Dwiputranto Uki Dwiputranto Uki Dwiputranto Wira Dhyaksa Pradana Yuliawati, Nasriyatun