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METODE ISOLASI PYRICULARIA ORYZAE PENYEBAB PENYAKIT BLAS PADI Wicaksono, Danar; Wibowo, Arif; Widiastuti, Ani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Isolation method of Pyricularia oryzae the causal agent of rice blast disease. Rice blast disease is a disease that reduces rice productivity and threatens global food reserves. The study of diversity and distribution of race fungal causing rice blast disease required a fungal isolates collection from different places and times. One of the challenges in collecting these fungi is the difficulty of isolation process. The purpose of this research was to study the proper isolation method of rice blast pathogen. The most appropriate isolation method of Pyricularia oryzae was to moisten the infected panicle, place on moist filter paper in a petri dish, and incubate plate for 2 days at room temperature under fluorescent lamp. Afterward, conidium was picked using sterile needle and transfered to potato dextrose agar without lactic acid.

Diseases and Pests Associated with Avocado Plantation Intercropping with Mango and Durian in Watulembu, Wonogiri Poerwanto, Mofit Eko; Wicaksono, Danar; Ulilalbab, Azizah Ridha; Ajri, Miftahul
Jurnal Agrivet Vol 30, No 2 (2024): AGRIVET (In Progress)
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v30i2.13049

One of strategic fruit commodities was avocado that had high economic value. Pest and diseases were main problems faced by farmers. This study identified diseases and pest associated with avocado plantation intercropping with mango and durian in Watulembu district, Wonogiri Regency through symptoms and its controls. The observation was done to the abnormalities avocado plants and the field condition surrounding the planting area. It was conducted by visual observation, recorded using a digital camera, and then described and identified based on their symptoms and a literature review. The results of our observation were Algal leaf spot, Anthracnose, Sooty mold, and Planococcus citri. Pest and plant disease could be controlled by cultural technique, avoid plant stress, and also using chemical pesticide.

PENGARUH PELAPISAN KITOSAN DAN TRICHODERMA TERHADAP SIFAT FISIOKIMIA CABAI MERAH KERITING SELAMA PENYIMPANAN SUHU RUANG Kafiya, Maftuh; Wicaksono, Danar
Jurnal Agrivet Vol 29, No 1 (2023): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v29i1.9996

Produk pertanian seperti cabai merah keriting adalah produk perishable yaitu memiliki karakteristik yang mudah rusak dan melimpah saat panen sehingga perlu penanganan yang baik terkait pasca panennya. Teknik pengendalian berbasis sumber daya hayati nasional dan ramah lingkungan diperlukan untuk mengurangi residu penggunaan bahan kimia dan meningkatkan umur simpan produk secara alami. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh sifat fisik cabai merah terhadap pelapisan kitosan dan isolate Trichoderma selama penyimpanan. Percobaan dirancang dengan rancangan acak lengkap (RAL) dengan 3 kali ulangan yang terdiri atas 5 perlakuan pelapisan, yaitu kontrol, kitosan 2,5%, Trichoderma, kitosan 2,5% + Trichoderma dan fungisida yang disimpan pada suhu ruang. Hasil penelitian menunjukkan bahwa lama penyimpanan cabai merah keriting berpengaruh nyata terhadap susut bobot, kadar air dan kadar vitamin C. Kombinasi pelapisan isolate Trichoderma + kitosan pada cabai merah keriting dengan penyimpanan suhu ruang mampu menekan kehilangan vitamin C dan susut bobot sebesar 16,7% sampai hari ke-8 penyimpanan.

INVENTORY OF RED CHILI (CAPSICUM ANNUM L.) PLANT DISEASES IN PAKEM DISTRICT, SLEMAN REGENCY Ulilalbab, Azizah Ridha; Ajri, Miftahul; Poerwanto, Mofit Eko; Wicaksono, Danar
Journal TECHNO Vol 10, No 1 (2024)
Publisher : Universitas Pembangunan Nasional Veteran Yogayakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Red chili is cultivated practically everywhere and plays a crucial role in Indonesian culture, economy, and cuisine. Plant diseases have been the main factor driving the country’s yield reduction of red chili. This research was conducted to observe chili plant diseases in Hargobinangun Subdistrict, Pakem District, Sleman, Yogyakarta. Chili variety Twist-42 was planted in the area. The Twist-42 variety is a local variety that is well adapted in the middle plains between 450 – 650 masl (meters above sea level) and is a good variety in both the dry and rainy seasons. There were 360 red chili plants as samples. The diseases found are Yellow leaf curl disease, Anthracnose, Ralstonia wilt, and Root knot nematode (Meloidogyne spp.).

Penghambatan Berbagai Isolat Trichoderma sp. Terhadap Perkecambahan Spora Colletotrichum sp. Wicaksono, Danar; Kafiya, Maftuh
Jurnal Agro Wiralodra Vol. 5 No. 1 (2022): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v5i1.74

Penyakit antraknosa pada buah cabai menyebabkan kualitas hasil menurun dan kehilangan hasil yang tinggi. Penyakit ini disebabkan oleh jamur Colletotrichum sp. Pada umumnya, antraknosa pada cabai dikendalikan dengan aplikasi fungisida kimia sintetis pada seluruh permukaan buah. Hal ini sangat berbahaya bila dilakukan menjelang panen karena buah cabai juga dikonsumsi segar. Agens pengendali hayati merupakan alternatif pengendalian Colletotrichum sp. pada buah cabai. Penelitian bertujuan untuk memperoleh isolat agens pengendali hayati yang dapat menghambat perkecambahan spora Colletotrichum sp. penyebab penyakit antraknosa pada buah cabai besar keriting. Penelitian diawali dengan isolasi Colletotrichum sp. dari cabai rawit, besar, dan merah keriting. Isolasi menghasilkan dua buah isolat dari cabai merah besar, 5 isolat diisolasi dari cabai merah keriting, dan 7 isolat dari cabai rawit. Uji virulensi dilakukan untuk memperoleh isolat yang paling virulen terhadap cabai merah keriting. Isolat BA asal cabai besar diketahui memiliki virulensi paling tinggi dibandingkan yang lainnya. Tujuh Trichoderma, 1 Gliocladium, dan 2 APH yang belum diidentifikasi digunakan biakan dalam media cair. Suspensi APH digunakan untuk mengendalikan penyakit antraknosa pada buah cabai merah keriting. Penambahan suspensi LPT2 menyebabkan persentase perkecambahan paling sedikit.

Olfactory Response of Diaphorina citri to Guava Leaves Powder Poerwanto, Mofit Eko; Solichah, Chimayatus; Wicaksono, Danar; Ulilalbab, Azizah Ridha; Ajri, Miftahul
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.96847

Citrus Vein Phloem Degeneration (CVPD) is transmitted by the insect vector Diaphorina citri is still a major obstacle to world citrus production. Vector handling still relies on chemical insecticides which are not environmentally friendly and less effective. This study was conducted to identify the ability of dried guava leaf extract in reducing the olfactory response of D. citri. Y-tube olfactory test was conducted to identify the repellency effect of a mixture of citrus leaf extract and guava (Psidium guajava) leaf extract at various leaf ages and in various proportions to vector and its predator (Menochilus sexmaculatus). The results showed that guava leaves had a repellent effect on D. citri, but not on M. sexmaculatus. The repellant effect was higher on young guava leaves than on medium and old ones. Red guava leaves have a highest repellant effect than white guava and non-seed guava leaves. Guava leaf extract is an alternative means to control D. citri. However, the types of compounds that function as repellants need to be studied further.

Keanekaragaman dan Peran Fungsional Jamur Rhizosfer Tanaman Lada (Piper nigrum L.) Berdasarkan Pendekatan Metagenomik di Banjarnegara, Jawa Tengah Wicaksono, Danar; Poerwanto, Mofit Eko; Ulilalbab, Azizah Ridha; Ajri, Miftahul; Kawuryan, Siwi Hardiastuti Endang
Jurnal Agro Wiralodra Vol. 8 No. 2 (2025): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v8i2.147

Black pepper (Piper nigrum L.) is a high-value spice crop whose productivity is strongly influenced by plant health and the microbial conditions in the rhizosphere. This study aimed to characterize the fungal communities in the rhizosphere of healthy black pepper plants grown in fields with a history of pest and disease outbreaks, using a metagenomic approach based on ITS1 sequencing via the Illumina platform. Rhizosphere soil samples were collected from healthy black pepper plants in a plantation located in Tribuana Village, Banjarnegara, Central Java, which had experienced pest and disease incidences in the previous growing season. Taxonomic composition, relative abundance, and diversity indices (Shannon, Simpson, and Richness) were analyzed. The results revealed that the fungal community was dominated by organic matter- decomposing fungi (57.29%), followed by plant growth- promoting fungi (PGPF) at 19.82%. These two groups not only exhibited quantitative dominance but also showed high diversity and evenness values, indicating ecological stability within the root system of healthy pepper plants. These findings highlight the importance of cultivation practices that support the persistence of functional microbial communities, such as organic matter amendments and the application of biofertilizers. In conclusion, the high dominance and diversity of decomposer fungi and PGPF in the rhizosphere of healthy black pepper plants underscore their critical role in maintaining plant health and provide a scientific basis for sustainable soil management strategies. Pathogenic fungal communities accounted for 12.76% of the total abundance, exhibiting moderate diversity and relatively high evenness.

INVENTORY OF RED CHILI (CAPSICUM ANNUM L.) PLANT DISEASES IN PAKEM DISTRICT, SLEMAN REGENCY Ulilalbab, Azizah Ridha; Ajri, Miftahul; Poerwanto, Mofit Eko; Wicaksono, Danar
Journal TECHNO Vol. 10 No. 1 (2024): Mei
Publisher : Universitas Pembangunan Nasional Veteran Yogayakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/journal techno.v10i1.12475

Red chili is cultivated practically everywhere and plays a crucial role in Indonesian culture, economy, and cuisine. Plant diseases have been the main factor driving the country’s yield reduction of red chili. This research was conducted to observe chili plant diseases in Hargobinangun Subdistrict, Pakem District, Sleman, Yogyakarta. Chili variety Twist-42 was planted in the area. The Twist-42 variety is a local variety that is well adapted in the middle plains between 450 – 650 masl (meters above sea level) and is a good variety in both the dry and rainy seasons. There were 360 red chili plants as samples. The diseases found are Yellow leaf curl disease, Anthracnose, Ralstonia wilt, and Root knot nematode (Meloidogyne spp.).

PENGARUH PELAPISAN KITOSAN DAN TRICHODERMA TERHADAP SIFAT FISIOKIMIA CABAI MERAH KERITING SELAMA PENYIMPANAN SUHU RUANG Kafiya, Maftuh; Wicaksono, Danar
Jurnal Agrivet Vol 29 No 1 (2023): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v29i1.9996

Produk pertanian seperti cabai merah keriting adalah produk perishable yaitu memiliki karakteristik yang mudah rusak dan melimpah saat panen sehingga perlu penanganan yang baik terkait pasca panennya. Teknik pengendalian berbasis sumber daya hayati nasional dan ramah lingkungan diperlukan untuk mengurangi residu penggunaan bahan kimia dan meningkatkan umur simpan produk secara alami. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh sifat fisik cabai merah terhadap pelapisan kitosan dan isolate Trichoderma selama penyimpanan. Percobaan dirancang dengan rancangan acak lengkap (RAL) dengan 3 kali ulangan yang terdiri atas 5 perlakuan pelapisan, yaitu kontrol, kitosan 2,5%, Trichoderma, kitosan 2,5% + Trichoderma dan fungisida yang disimpan pada suhu ruang. Hasil penelitian menunjukkan bahwa lama penyimpanan cabai merah keriting berpengaruh nyata terhadap susut bobot, kadar air dan kadar vitamin C. Kombinasi pelapisan isolate Trichoderma + kitosan pada cabai merah keriting dengan penyimpanan suhu ruang mampu menekan kehilangan vitamin C dan susut bobot sebesar 16,7% sampai hari ke-8 penyimpanan.

Diseases and Pests Associated with Avocado Plantation Intercropping with Mango and Durian in Watulembu, Wonogiri Poerwanto, Mofit Eko; Wicaksono, Danar; Ulilalbab, Azizah Ridha; Ajri, Miftahul
Jurnal Agrivet Vol 30 No 2 (2024): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v30i2.13049

One of strategic fruit commodities was avocado that had high economic value. Pest and diseases were main problems faced by farmers. This study identified diseases and pest associated with avocado plantation intercropping with mango and durian in Watulembu district, Wonogiri Regency through symptoms and its controls. The observation was done to the abnormalities avocado plants and the field condition surrounding the planting area. It was conducted by visual observation, recorded using a digital camera, and then described and identified based on their symptoms and a literature review. The results of our observation were Algal leaf spot, Anthracnose, Sooty mold, and Planococcus citri. Pest and plant disease could be controlled by cultural technique, avoid plant stress, and also using chemical pesticide.

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