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All Journal PROSIDING SEMINAR KIMIA JURNAL INDRIA (Jurnal Ilmiah Pendidikan Prasekolah dan Sekolah Awal) JURNAL ATOMIK
Winni Astuti, Winni
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science

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Journal : PROSIDING SEMINAR KIMIA

 1 
AMPLIFICATION OF pncA GENES FROM WATER BACTERIAL POST MINING IN SAMARINDA Suryadi, Ahmad; Astuti, Winni; Kartika, Rudi
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Amplification of pncA genes from water bacterial post mining in Samarinda has been done. The aim of this research is to isolate chromosomal DNA and amplificated the pncA gene from water bacterial post mining in Samarinda. The research methode consist of chromosomal DNA isolation and amplification of pncA gene with polymerase chain reaction (PCR) technique. The result showed the size of chromosomal DNA is ± 10 kb. By using primer pncA gene, the result of amplification of the pncA gene showed that there were two sizes of amplicon, namely 0.7 kb and 0.2 kb. The conclusion that can be drawn was there is two sizes of amplicon pncA gene. Keywords: chromosomal DNA isolation, amplification of pncA gen, PCR
TEST OF AMYLASE INHIBITION ACTIVITY OF METHANOL EXTRACT SINGKIL LEAF (Premna cordifolia Roxb.) Anggriani, Yusnia Wati; Astuti, Winni; Marliana, Eva
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Singkil (Premna cordifolia Roxb.) is a plant that is widely found un Kalimantan. The people of Kalimantan are often used singkil leaf by as traditional medicine are believed to have medicinal properties as a cure for intestinal worms, colds, and antidiabetic. In this research, it used phytochemical screening and inhibition activity amylase test on singkil’s methanol extract. The objective of this study was to determine in-vitro enzyme amilase inhibition activity against sampel by DNS method. The result of this research was phytochemical screening in singkil’s methanol extract contains alkaloids, flavonoids, saponins and phenolics. Singkil leaf methanol extract at a concentration of 10 µg/mL can inhibit the activity of the amylase enzyme with an inhibition value of 60,93 %. Acarbose used as a positive control in this study showed activity against the amylase of 44,79 %. Amylase inhibition activity of methanol extract was higher than acarbose, the singkil leaf methanol extract has the potential as an antidiabetic drug. Keywords: Premna cordifolia Roxb., Amylase inhibition, Secondary Metabolites.
OPTIMATION TIME PRODUCTION SECONDARY METABOLITES ENDOFIT BACTERIES EXTRACT OF PUCUK MERAH LEAVES (Syzygium myrtifolium Walp.) Rahimah, Priliza; Astuti, Winni; Marliana, Eva
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Pucuk Merah (Syzygium myrtifolium Walp.) is an ornamental plant that has benefits as an antioxidant, antibacterial, antiplatelet, antiulcerative, antimalarial, antiviral, antihyperglycemic and anti-inflammatory. This research aims to determine the optimum time for the production of secondary metabolites of pucuk merah endophytic bacteria for amylase and protease inhibition. Production of secondary metabolites was carried out at various times of 24 hours, 48 ​​hours and 72 hours. The method for the amylase inhibition test used the DNS method and the protease inhibition test used the Bradford method. The results showed that the optimum time for the production of secondary metabolites was 48 hours. Keywords: Syzygium myrtifolium Walp., Endophyte Bacteria, Production Time.
THE POTENTIAL OF EXTRACT SINGKIL LEAVES (Premna cordifolia Roxb.) AS ANTIBACTERIAL AGENTS AGAINST Propionibacterium acnes Az-zahra, Deannisa Khoiru; Astuti, Winni; Marliana, Eva
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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One of the causes of acne is infection by the bacteria Propionibacterium acnes. Singkil (Premna cordifolia Roxb.) is a kind of herb plant from Kutai Kartanegara which is used as a traditional herb including for acne’s treatment. This study aims to know the content of secondary metabolites compound in singkil’s extract and determine the antibacterial activity against Propionibacterium acnes KCCM 41747 bacteria. In this study, it’s phytochemical screening and antibacterial activity test using the disc diffusion method on methanol extract, n-hexane, ethyl acetate and water-methanol fractions. the result of phytochemical screening revealed that methanol extract of singkil contains alkaloids, flavonoids, steroids, and saponins. The n-hexane fraction contains steroids and the ethyl acetate fraction contains alkaloids, flavonoids, and phenolics while the water-methanol fraction contains alkaloids, flavonoids, and phenolics. The antibacterial activity test extract with a concentration of 10% showed that the diameter of clear zone of the metanol extract, n-hexane fraction, ethyl acetate fraction, and water-methanol fraction were 13 mm, 9 mm, 13,67 mm and 6 mm, respectively. The ethyl acetate fraction had the highest antibacterial activity among the four extracts. A minimum inhibitory concentration value of the ethyl acetate fraction against Propionibacterium acnes KCCM 41747 was 0,625%. The ethyl acetate fraction have the potential as an antibacterial against the bacteria Propionibacterium acnes KCCM 41747. Keywords: Premna cordifolia Roxb., Secondary metabolites, Propionibacterium acnes KCCM 41747
PENGARUH ION LOGAM TERHADAP AKTIVITAS LIPASE DARI BAKTERI AIR BEKAS GALIAN TAMBANG DI SAMARINDA Haeruddin, Haeruddin; Astuti, Winni; Pratiwi, Djihan Ryn
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Addition of metal ions on lipase activity has been done. The aim of this research is to find out the effect of metal ions on lipase activity from water bacterial post mining in Samarinda. Lipase activity was determined based on the Titrimetric method with olive oil as a substrate. The result showed lipase activity from water bacterial post mining without the addition of metal ions is 1,0 U/mL and with the addition of metal ions Mg2+, K+, Fe3+, Ca2+, dan Ba2+their respective lipase activities is 0,6 U/mL, 0,7 U/mL, 1,9 U/mL, 1,4 U/mL dan 0,8 U/mL. The conclusion that can be drawn that is metal ions K+, Mg2+ and Ba2+ inhibit lipase activity with a relative activity each at 70; 60 dan 80% while Ca2+ increase lipase activity with a relative activity at 140% and Fe3+ causes denatured lipase with a relative activity at 190%. Keywords: Bacteria, Lipase, Metal Affect
Co-Authors A. A. R, Muhammad Taufik Admaja, Junior Try Afandi, Irvan Ahmad Suryadi, Ahmad Akbar Akbar Alawiyah, Dessy Noor Amalia, Elfiza Amantha, Dhita Novalia Anggriani, Yusnia Wati Aristina, Reza Fauziah Arwidhiah, Nanda Az-zahra, Deannisa Khoiru Chairul Saleh Djihan Ryn Pratiwi, Djihan Ryn Erwin Erwin Eva Marliana, Eva Fahmi, Ismi Haeruddin Haeruddin Hairani, Rita Kesuma, Yonanda Meisya Kusumawardani, Niken Marliani, Nelly Mikdariawan, Adha Mutma Inna Nasution, Safridah Hannum Noor Hindryawati, Noor Nur, Istianti Irfa'a Nursanti, Oktavia Widya Panuti, Putri Rahayuning puah, Siti Mar Purnamasari, Ade Rezki Putri, Amanda Aulia Rabiatul Adawiyah Rahimah, Priliza Rahmaniah, Rahmaniah Rakasiwi, Dea Rudi Kartika, Rudi Ruga, Ritbey Saibun Sitorus Salsabila, Ervina Shafarini, Eno Julia Siti Nurjanah Sya'bani, Nurillah Trisnawati, Eka Elly Usdin, Muhammad Sadam Utami, Nanda Putri Vera, Hendrica Mini Wahyu Pratama