Article Per Year (5 Year)
p-Index From 2020 - 2025
1.048
P-Index
This Author published in this journals
All Journal Meditory : The Journal of Medical Laboratory Jurnal Info Kesehatan
Martsiningsih, Marta Atik
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
ALTERNATIVE MEDIA FOR BACTERIAL GROWTH STAPHYLOCOCCUS AUREUS: SWEET POTATO INFUSA (IPOMOEA BATATAS), SOYBEANS (GLYCINE MAX (L) MERRILL) AND YEAST EXTRACT Martsiningsih, Marta Atik; Astuti, Fardhiasih Dwi; Sistiyono, Sistiyono; Suyana, Suyana; Rahmawati, Ullya; Rahayu, Muji; Musyafaah, Novita Siti
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3081

Abstract

Background: Staphylococcus aureus needs nourishment for its growth. The needed nutrients of carbohydrates, proteins, nitrogen, vitamins, and minerals from the media. The media commonly used for bacterial growth is the nutrient medium for high prices. Alternative media from natural local foodstuffs are obtained easily and cheaply instead of instant media. This study aims to identify alternative media mixed with infuse sweet potato (ipomoea batatas), soybeans (glycine Max (l) Merrill), and yeast extract that can be used to grow the staphylococcus aureus bacteria.  Methode: this type of research is pure experimental research, and the design used is the posttest-only control design group. Staphylococcus aureus suspension was grown on alternative media and nutrient agar media as a comparison. measurements of colony diameter and number of colonies were compared between alternative media and nutrient agar. Result The results Research has provided an average number of staphylococcus aureus bacteria that grow on alternative media 78.00 x 104 CFU /ml and nutrient media for 94.19 x 104 CFU /ml. Whereas the colony's diameter of alternative media 2.56 mm, and the media nutrient increased by 2.66 mm. Conclusion: Alternative media from a blend of yam, soy infuse, and yeast extract can be used for the growth of the staphylococcus aureus bacteriaKeywords: Staphylococcus aureus; Alternative Media; Potato Infusa; soy infuse; soy infuse
Comorbid Description and Neutrophil Lymphocyte Ratio in COVID-19 Patients (Suspect and Confirmed Patients) Widada , Subrata Tri; Martsiningsih, Marta Atik; Supriyanta, Bambang; Sistiyono; Suyana; Nuryani, Siti; Setiawan, Budi; Sulistyowati, Dwi; Muji Rahayu
JURNAL INFO KESEHATAN Vol 20 No 2 (2022): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol20.Iss2.1002

Abstract

Neutrophil Lymphocyte Ratio (NLR) is a laboratory available as a marker used for the evaluation of systemic inflammation, NLR is a significant predictor and is a critical prognosis for COVID-19 infection and can serve as a useful factor to reflect the intensity of the imbalance of inflammation and immune response in COVID-19 patients. This study aims to determine the difference in NLR values in negative and confirmed COVID-19 patients and description for comorbid for both. This study is an analytic observational study with a cross-sectional design. The study sample was 423 suspected COVID-19 patients at hospitals in Cilacap district for the period in March – October 2020. The data obtained were analyzed descriptively and using the fisher-exact test. In these results from suspected patients with negative COVID-19, lung illness were present 31.8%, viral infections 22.9%, other respiratory disorders 6.1%, diabetes mellitus 4.7%, and anemia 4.7%. Whereas suspected patients with confirmed COVID-19 were, without comorbid diseases (40.2%), lung disease (12.4%), diabetes mellitus (7.7%), hypertension (6.2%), and other respiratory illnesses (5.2%). The mean of NLR in confirmed patients is 3.57 but not any difference between negative and confirmed patients COVID-19, but there’s any a relationship between NLR and ARDS conditions.
Effect of The Temperature on The Size of Inhibition Zone of the Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim Activity Against Staphylococcus aureus ATCC 25923 Sistiyono, Sistiyono; Martsiningsih, Marta Atik; Kasiyati, Menik; Rahayu, Muji; Pudyastuti, Rita Rena; Suyana, Suyana; Rahmawati, Ully; Rahmad; Faruq, Zulfikar Husni; Setiawan, Budi
JURNAL INFO KESEHATAN Vol 22 No 3 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss3.1353

Abstract

Antibiotic sensitivity testing is essential for determining bacterial susceptibility to antibiotics. In disc diffusion testing, several technical factors influence the diameter of the inhibition zone, including incubation temperature, which must be carefully controlled to ensure the validity of test results. This study aims to determine the mean, difference, and analyze the diameter of the inhibition zone of the antibiotics, namely Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim against Staphylococcus aureus on Mueller-Hinton agar media with incubation temperatures of 33°C, 34°C, 35°C, 36°C and 37°C for 18 hours. This research is observational, with a cross-sectional design. The primary data is 100 data on the diameter of the antibiotic inhibition zone, obtained by measuring the diameter of the inhibition zone with different incubation temperatures. The selection of antibiotics is based on the mechanism of action of antibiotics inhibiting bacteria, namely the cell wall or cell membrane that surrounds the bacterial cell; the pieces of machinery that make the nucleic acids DNA and RNA, and the machinery that produces proteins (the ribosome and associated proteins) with a range of inhibition zones based on Internal Quality Control CLSI. The data and the repeated measure statistical test will be processed univariately to determine the significance of the difference in the diameter of the formed inhibition zone using the ANOVA test. The measurement of the inhibition zone diameter on the incubation temperature variation showed a significant difference with a p-value of 0.000 for Levofloxacin, Tetracycline, and Trimethoprim, while the p-value of Clindamycin is 0.010. For the other antibiotics, Levofloxacin, Tetracycline, and Trimethoprim antibiotics, the higher the incubation temperature, the average diameter of the inhibition zone is condensed, while for Clindamycin the higher the incubation temperature, the higher the average diameter of the inhibition zone is the same. Incubation temperature and volume affect the diameter of the antibiotic inhibition zone in the disc diffusion method for the antibiotic sensitivity test. It can be concluded that incubation temperature affects the diameter of the antibiotic inhibition zone in disc diffusion tests. It is recommended for future standardized and precise testing conditions to ensure accurate and reliable antibiotic sensitivity results.              
Co-Authors Bambang Supriyanta, Bambang Budi Setiawan Dwi Sulistyowati Fardhiasih Dwi Astuti Kasiyati, Menik Muji Rahayu Muji Rahayu Musyafaah, Novita Siti Pudyastuti, Rita Rena Rahmad Rahmawati, Ully Sistiyono Sistiyono Sistiyono, Sistiyono Siti Nuryani, Siti Suyana Suyana Suyana, Suyana Ullya Rahmawati Widada , Subrata Tri Zulfikar Husni Faruq