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All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Parasite Science
Wiwik Misaco Yuniarti
Divisi Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Variasi Morfologi dan Deteksi Leucocytozoon caulleryi dengan Metode PCR pada Ayam Ras di Wilayah Endemis Indonesia Endang Suprihati; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8800.643 KB) | DOI: 10.22146/jsv.34666

Abstract

The purpose of this study was to analyze the variation in morphology and detection Leucocytozoon caulleryi by PCR in mtDNA genes cty b that attacking bred chickens in endemic areas of Indonesia. This study was divided into three stages, data collection of Leucocytozoonosis cases in endemic areas of Indonesia; identification of parasites Leucocytozoon caulleryi morphologically through microscopic examination; and the identification of Leucocytozoon caulleryi cyt b genes that attack bred chickens in endemic areas by PCR. The results showed that there are variations in morphology of Leucocytozoon caulleryi that attacking chicken in endemic areas of Indonesia. Gamete morphometry of L. caulleryi had an average length and width 18.233 ± 4.672 dan 12.934 ± 3.349 µm. Nested PCR clearly showed positive reaction of Leucocytozoon infections by amplicons in 600 bp and 03 bp length. 
Pengaruh Ekstrak Buah Delima Terstandar 40% Ellagic Acid terhadap Profil Darah Tikus Putih Yang Mengalami Nefrotoksisitas akibat Induksi Gentamisin Bambang Sektiari Lukiswanto; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7994.355 KB) | DOI: 10.22146/jsv.34697

Abstract

The purpose of this study was to determine the effect of pomegranate extract of 40% ellagic acid on blood profile of albino rats that had nephrotoxicity after included with Gentamicin. a total of 32 white rats were divided into four treatment groups, each consisting of eight rats. the four treatment groups were healthy rats (P0), Gentamicin induced rats (P1), Gentamicin induced rats and ellagic acid treatment (P2) and Gentamicin induced rats and standardized Pomegranate extract therapy 40% ellagic acid treatment (P3). After seven days of treatment, blood was taken for blood profile tests. The results showed that in the Gentamicin-induced group (P0), a decrease in blood profile allegedly caused by Gentamicin that could cause damage and impaired renal tubular function. Givibg ellagic acid and pomegranate extracts of 40% ellagic acid proved to improve the blood profile of Gentamicin induced rats. This is due to inhibition of renal damage and improved renal function due to antioxidant activity possessed by ellagic acid and pomegranate extract of standardized 40% ellagic acid.
Efektifitas perlakuan awal Tretinoin 0,1% Topikal pada Proses Penyembuhan di Luka Insisi pada Mencit (Mus musculus) Iswinarno Doso Saputro; Hesty Aulia Palupi; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9452.348 KB) | DOI: 10.22146/jsv.41152

Abstract

Tretinoin has an important role in wound healing process include improve fibroplasia and collagen synthesis, maintening humoral immunity, and neutralize steroid effect.Epithelization  and fibroblast proliferation were very important event in wound healing process. The aims of this study were studying epithelization and fibroblas proliferation on incision wound healing process using pre treatment of  Tretinoin 0,1%  topically on mice. This research used 24 male mice, 3 month of age, with 150 -180 gram of body weight. The mice were divided into 2 groups, control group (K) and treatment group (P). The control group was a group of mice with an incision wound, while the treatment group was a group of mice with an incision wound that received a pretreatment of Tretionin of 0.1% topically. Each group was divided into 2 sub groups K4, K7, P4, P7 based on time of samples collecting. K4 and P4 were examined at  day 4, while K7 and P7 were examined at day 7. Epithelialization was observed by measuring the length of the epithelial incision area, whereas fibroblast proliferation was performed by counting the number of fibroblasts in five field of view on histopathologic skin preparations processed by HE staining.In this study,pretreatment of Tretinoin 0,1%  topically can increase epithelialization and number of fibroblast significantly (p<0,05).This increase may be due to 1% Tretinoin in the tissues to induce keratinocyte basal proliferation including its migration to the skin surface via retinoic acid receptors ( RARδ)Pretreatment of Tretinoin 0,1% topically can increased epithelialization and number of fibroblast on incision wound healing process of albino rats
Terapi Fotodinamik Mempercepat Kesembuhan Luka Insisi pada Kulit Tikus Putih (Rattus novergicus) yang Diinfeksi Bakteri Methicilin-Resistant Staphylococcus aureus Yonatan Dimascahyo Budianto; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Hani Plumeriastuti; Wiwiek Tyasningsih; Boedi Setiawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.591 KB)

Abstract

The aims of this research was to determine the effect of photodynamic therapy (PDT) on the healing of incision wounds in rat skin (Rattus norvegicus) that infected with MRSA bacteria by evaluating and give a score (0 – 4) at wound healing factors such as epithelialization, angiogenesis, inflammatory cells, fibroblast cells and collagen density. This study used 25 white rats divided randomly into 5 groups with 5 replications, namely P0 = MRSA infection wound + Povidone Iodine, P1 = MRSA infection wound + Silver Sulfadiazine, P2 = MRSA infection wound + 5-minute PDT irradiation, P3 = MRSA infection wound + 10- minute PDT irradiation and P4 = MRSA infection wound + 15-minute PDT irradiation. Histopathological observations and scoring showed epithelialization and collagen density in groups P2, P3 and P4 increased significantly compared to groups P0 and P1 (p<0.05). The histopathological observations and scoring of angiogenesis, inflammatory cells, fibroblast cells of P0 and P1 groups had a higher score than the treatment group with photodynamic therapy P2, P3 and P4 (p<0.05). This condition showed that in the P0 and P1 groups are still in the inflammatory phase, while in the P2, P3 and P4 groups have entered the remodeling phase which is characterized by high epithelialization scores and collagen density. Giving photodynamic therapy to wounds infected with MRSA can accelerate the wound healing process.
Deteksi Molekuler Blastocystis sp. pada Babi Terinfeksi di Kabupaten Tabanan dan Badung, Provinsi Bali, Indonesia (MOLECULAR DETECTION OF BLASTOCYSTIS INFECTION IN PIGS AT TABANAN AND BADUNG DISTRICT, BALI PROVINCE, INDONESIA) Doohan Mahendra; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Endang Suprihati; Wiwik Misaco Yuniarti; Ni Komang Aprilina Widisuputri
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.288 KB)

Abstract

This study aims to identify morphology and molecular of Blastocystis infection in pigs in the Tabanan and Badung District, Bali Province, Indonesia. 100 samples of pig stool were taken, fifty samples from the Tabanan and fifty samples from Badung District. Blastocystis sp. isolated and identified microscopically and confirmed by polymerase chain reaction (PCR) as a molecular identification method. 63% (63/100) Blastocystis is found in pig feces from these two District by microscopic detection. Positive samples were found 27 samples from Badung and 36 samples from Tabanan. Five samples were taken from each District conducted a confirmation test using the PCR method and found 60% (6/10) positive samples, two samples from Badung and four samples from Tabanan. The results of this study indicate that in healthy pigs, they also contain parasites that have the potential to spread disease to humans. Therefore, the high incidence of Blastocystis in pigs can be an alarm or warning to prevent Blastocystis infection from livestock that can potentially to be a reservoir in the spread of disease.
Potensi Salep Epigallocatechin gallate terhadap Proses Kesembuhan Luka Bakar Derajat II pada Kulit Tikus Putih (THE POTENTIAL OF EPIGALLOCATECHIN GALLATE OINTMENT TO THE WOUND HEALING PROCESS OF SECOND DEGREE SKIN BURNS ON THE ALBINO RATS) Wiwik Misaco Yuniati; Bambang Sektari Lukiswanto
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.331 KB) | DOI: 10.19087/jveteriner.2019.20.1.1

Abstract

Burns are one of the health problems in modern society that are associated with tissue damage that is difficult to repair and affect patients, both physically and psychologically. This study was conducted to evaluate the potential of epigallocatechin gallate (EGCG ) ointment to the healing process of second degree skin burn induced by attaching 85ºC plate with 1 cm of diameter for 5 second on the skin of albino rat (Rattus norvegicus). Twenty-five rats were divided into 5 treatment groups. The P0 group was a group of rat that suffered burns and were treated with ointment base (PEG). The P1 group was a group of rat that suffered burns and were given standard therapy with silver sulfadiazine. P2, P3 and P4 groups are groups of rat that have burns and are treated with EGCG ointments with concentrations of 1%, 2%, and 4% respectively. At the end of the study, skin tissue excision was carried out to make histopathological preparations using HE staining. Evaluation of histopathological preparations was carried out on reepithelialization collagen deposition, PMN infiltration, and angiogenesis. The results of the study in group P4 showed that the highest collagen formation and re-epithelialization process was accompanied by a marked decrease in the inflammatory process and angiogenesis. This condition is significantly different from groups P0, P1, P2, and P3. Healing second degree burns with 4% EGCG is better than other treatmentJurnal Veteriner Maret 2019 Vol. 1 No. 1 : 1-7 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.1 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/20162Proses kesembuhan luka bakar merupakan fenomena kompleks untuk mengembalikan kontinuitas jaringan dan fungsinya. Kesembuhan luka melibatkan beberapa fase yang berbeda dan saling tumpang tindih, yaitu fase inflamasi, granulasi, fibrogenesis, neo-vaskularisasi, kontraksi luka dan epithelialisasi (Robson, 1997). Penatalaksanaan luka bakar yang efektif memerlukan pemahaman proses kesembuhan luka normal dan mampu memilih intervensi yang tepat untuk mengoptimalkan proses kesembuhan luka (Snyder, 2005). Pada proses kesembuhan luka, inflamasi terjadi segera setelah jejas, diawali dengan vasokonstriksi yang berperan dalam proses hemostasis dan pelepasan mediator inflamasi. Fase proliferasi ditandai dengan terbentuknya jaringan granulasi oleh fibroblas dan proses angiogenesis. Reformulasi dan perbaikan kompartemen serabut kolagen yang disertai dengan penigkatan tensile strength menandai fase remodeling (Varoglu et al., 2010). Faktor yang memiliki peran penting pada tertundanya proses kesembuhan luka antara lain, trauma berulang, perfusi dan oksigenasi yang buruk serta inflamasi yang berlebihan (Harding et al., 2003)). Penggunaan bahan alamiah untuk pengobatan luka merupakan bagian penting dari penatalaksanaan kesehatan dan metode baik untuk menyediakan pilihan layanan kesehatan yang murah dan efektif (Gurung et al., 2009; Suntar et al., 2010). Beberapa penelitian menggunakan polifenol yang berasal dari teh hijau sebagai penyembuh alami sebagai agen anti penuaan, antiinflamasi, antikanker, antioksidan dan antidiabetes (Obaid et al., 2011). Beberapa penelitian dengan menggunakan Epigallocatechin gallate (EGCG), sebagai salah satu polifenol yang terkandung dalamteh hijau, membuktikan sejumlah efek biologis EGCG sebagai antioksidan, antimikrob, antiinflamasi, antialergi dan antineoplastik yang aktif (Hosnuter et al., 2015). Teh hijau memiliki manfaat yang beragam, antara lain mencegah kanker, meningkatkan kesehatan jantung dan pembuluh darah, melindungi kulit dari kerusakan yang disebabkan karena radiasi dan penyebab yang lain. Manfaat ini disebabkan karena the hijau yang mengandung EGCG memiliki aktivitas antioksidan, antiinflamasi dan antibakteri yang cukup kuat. Berdasarkan hal tersebut, tidak menutup kemungkinan bahwa EGCG dapat membantu percepatan proses kesembuhan luka bakar pada kulit (Nagle et al., 2006). Berdasarkan uraian tersebut, penelitian ini dilaksanakan untuk melihat pengaruh pemberian salep EGCG terhadap proses kesembuhan luka bakar pada tikus putih (Rattus norvegicus) jantan.METODE PENELITIAN Kelayakan Etik Penelitian ini dilaksanakan setelah mendapatkan persetujuan dari komisi etik Fakultas Kedokteran Hewan, Universitas Airlangga. Tempat Penelitian Penelitian ini dilakukan di Laboratorium Hewan Percobaan di Fakultas Kedokteran Universitas Airlangga, Surabaya. Persiapan dan pembuatan salep dilakukan di Fakultas Farmasi, Universitas Airlangga. Pembuatan dan pemeriksaan preparat histopatologis dilakukan di Laboratorium Patologi Anatomi, Fakultas Kedokteran Hewan, Universitas Airlangga. Hewan Eksperimental Sebanyak 25 ekor tikus putih (Rattus norvegicus) jantan, umur tiga bulan dengangroups. This is presumably because the compounds contained therein have antioxidant activity, antiinflammatory and antibacterial. These three activities will synergize in the process of healing wounds. Provision of 4% EGCG ointment for 14 days in second degree burns can accelerate the wound healing process which is characterized by improved re-epithelialization, collagen deposition, PMN infiltration in the wound area, and angiogenesis.
Prevalance of Gastrointestinal Protozoa of Cats in Animal Hospital and Animal Clinic in Surabaya Amara Lintang Pagati; Lucia Tri Suwanti; Chairul Anwar Nidom; Wiwik Misaco Yuniarti; Sarmanu Sarmanu; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.385 KB) | DOI: 10.20473/jops.v2i2.16401

Abstract

Research of gastrointestinal protozoan in cats at Surabaya are still limited. Protozoa infection of the gastrointestinal tract can cause diarrhea and even zoonosis. This research aimed to identify and determine the prevalence of protozoan in cats in animal hostptal and animal clinic in Surabaya. Ninety  fecal samples were collected from 2 animal clinic and one animal hospital. Samples were  examined e wet mount (native, sedimentation, and floatation) and (Ziehl Nellsen) stain. Protozoa was identified by using a light microscope with 400x and 1000x magnification. The result showed 68,89% of samples were positively infected by gastrointestinal protozoa. The protozoa were Blastocystis sp, Cryptosporidium sp, Giardia sp, and Eimeria sp. By chi square test, there was  not significant differences the prevalence of gastrointestinal protozoan in cat between sex, age, breed, and diarrhea status
Utilization of Curcuma Molases Block to Increase The Performance of Goats that Infected Gastrointestinal Worm at Wonorejo, Wates District, Kediri Regency Hani Plumeriastuti; Poedji Hastutiek; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Nusdianto Triakoso; Arimbi Arimbi
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.314 KB) | DOI: 10.20473/jops.v2i2.16407

Abstract

This was a Community Service activity that aims to share knowladge about utilization of Curcuma Molasses Block (CMB) to increase performance of goats that infected gastrointestinal (GI) parasites to The Goat Breeder Group at  Wonorejo, Wates District, Kediri Regency. The activities include: counseling about raising goat management, presentasion on worm diseases that infect goats and CMB as anthelmentic, workshop and practice to make CMB, detection egg worm in goat`s feces, treatment on infected goats and filling out questionnaires evaluating the performance of goat before and after giving CMB. The result show that all of feces samples were positive contain egg worms of  Haemonchus contortus, Chabertina ovina, Trichuris globulosa, Strongyloides papillosus, Fasciola gigantica, Trichostrongylus spp., Toxocara sp., and Oesohagustomum columbianum even also protozoans  Eimeria sp., and Blastocystis sp. Administration CMB increase performance of worm-infected goats which indicated by increase in appetite, soft and shiny hair and the body more well-fed.
Detection of Blood Protozoa Infecting Broiler Chicken Farms in Tanjung Gunung Village, District Jombang Marchelia Arifiandani; Endang Suprihati; Wiwik Misaco Yuniarti; Nunuk Dyah Retno Lastuti; Poedji Hastutiek; Sunaryo Hadi Warsito
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.452 KB) | DOI: 10.20473/jops.v3i1.16422

Abstract

Broiler chicken farms could be a part of potential source infection Leucocytozoonosis and Plasmodiosis disease due to the death of livestock. Broiler farms in Tanjung Gunung, District Jombang near the fields, water, and trees. This could be increase the population of vectors infection Leucocytozoonosis and Plasmodiosis. The main purpose of this researchis to detect Leucocytozoonosis and Plasmodiosis infections on broiler farms in Tnjung Gunung Village, District Jombang using 50 broiler chickens by Purposive Sampling  of 2 bredeers. The result show rhat there are 6 positive samples infected Leucocytozoonosis, while Plasmodiosis infection could not be fond. Need cage sanitation to break life cycles of vector Leucocytozoonosis and Plasmodiosis.
The effects of Using Nano-Meniran (Phyllanthus niruri) Extract on the Feed Economic Value and Income Over Feed Costs of Broiler Chicken Farm Elita Triwijayanti Aqfari; Emy Koestanti Sabdoningrum; Sri Hidanah; Widya Paramita Lokapirnasari; Soeharsono Soeharsono; Wiwik Misaco Yuniarti
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 1 (2023): April 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.01.03

Abstract

This study aims to determined the effects of using nano-meniran (Phyllanthus niruri) extract on the feed economic value and income over feed costs of broiler chicken farms. This research method was an observational study with a sample of one-day-old Cobb strain broiler chicken at PT. Haraka Kitri Endah. A total of 79.600 day-old-chicken were divided into 4 groups of treatments and eight replications, each group of treatments consisted of 19.900 day-old-chicken. Treatments are given for 14 days – from the chickens are 21 days old until the chickens reach 35 days old consisted of T0, as the control group, was not given nano-meniran extract and was only given mineral feed; T1 was given nano-meniran extract 5%; T2 was given nano-meniran extract 10%; and T3 was given nano-meniran extract 20%. The administration of nano-meniran (Phyllanthus niruri) extract on the feed’s economic value and income over feed costs showed a significant difference (p<0.05). Research results showed the feed economic value of the broiler chickens are T0 = IDR 13.345,21; T1= IDR 12.315,57; T2 = IDR 13.057,11; and T3 = IDR 13.476,69. Income over feed costs calculations showed that T0 = IDR 8.942,13; T1= IDR 11.276,63; T2 = IDR 8.848,44; and T3 = IDR 8.299,44. T1 with nano-meniran extract 5% gives the best result. This research showed that nano-meniran extract has an immunomodulatory activity that positively impacts broiler chickens because it reduces the feed’s economic value and increases the income over feed costs.
Co-Authors Amara Lintang Pagati Arimbi Arimbi Bambang Sektari Lukiswanto Bambang Sektiari Lukiswanto Boedi Setiawan Doohan Mahendra Doohan Mahendra Edward Yonas Kristijanto Elita Triwijayanti Aqfari Emy Koestanti Sabdoningrum Endang Suprihati Endang Suprihati Hani Plumeriastuti Hesty Aulia Palupi Iswinarno Doso Saputro Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Mufasirin Ni Komang Aprilina Widisuputri Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poedji Hastutiek Puput Ade Wahyuningtyas Sarmanu, Sarmanu Soeharsono Soeharsono Sri Hidanah Tyasningsih, Wiwiek Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari Yonatan Dimascahyo Budianto