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Analysis Of Determining Flavonoid Contents In Red Onion (Allium Cepa L.) Extract Using Multiple Methods Arzak, La Ode Miftahul; Pratama, Mamat
Pharmaceutical Reports Vol 4, No 1 (2025): (March) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v4i1.297

Abstract

Shallots contain secondary metabolites such as flavonoids, kaempferol, flavonglycosides and quercetin. This research aims to conduct a literature study on the analysis of the levels of flavonoid compounds in shallots (Allium cepa L). This research uses a literature study where references are obtained through the Google Scholar and Elsevier websites, then articles are selected that match the criteria in the literature study. Based on the search carried out, 7 correct articles were obtained. From the results of the literature study, it can be concluded that the assay methods that can be used to determine the flavonoid content of shallots are High Performance Liquid Chromatography (HPLC) and UV-Vis Spectrophotometer. And there are also several flavonoid derivative compounds contained in shallots from published articles, such as quercetin and anthocyanin.
Determination of Total Flavonoid Content of Red Fruit Juice (Pandanus conoideus Lam) by a UV–Vis Spectrophotometer Sulaeman, Nur Rachmi; Pratama, Mamat; Kusuma, Andi Trihadi
Pharmaceutical Reports Vol 4, No 1 (2025): (March) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v4i1.360

Abstract

Red fruit (Pandanus conoideus Lam) belongs to the pandanacea family. This plant contains compounds such as tannins, flavonoids, steroids, triterpenoids and alkaloids and has properties in curing various diseases such as diabetes, coronary heart disease, hypertension, cancer and HIV/AIDS. This study aims to determine the total flavonoid content of red fruit juice (Pandanus conoideus Lam) using a UV - Vis spectrophotometer. The stages carried out in this research began with sample preparation, qualitative testing of flavonoid compounds and determining the total flavonoid content of red fruit (Pandanus conoideus Lam) samples. The results obtained in this study based on a qualitative test using NaOH reagent stated that red fruit juice (Pandanus conoideus Lam) was positive for containing flavonoids and in a quantitative test using a UV - Vis spectrophotometer the percent concentration obtained in the red fruit juice sample was 8.7258%.
The Antioxidant Activity of Red Fruit Juice (Pandanus conoideus Lam.) Using The FRAP Method Ainunnisa, Mufti; Fawwaz, Muammar; Pratama, Mamat
Pharmaceutical Reports Vol 4, No 1 (2025): (March) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v4i1.355

Abstract

Red fruit is a member of the Pandanaceae family which is a type of pandan. Empirically, red fruit (Pandanus conoideus Lam.) has been proven to cure various degenerative diseases such as cancer, heart disease, tuberculosis, respiratory tract disorders, external antiseptic and eye and skin diseases. The aim of this research was to determine the antioxidant activity of red fruit juice using the ferric reducing antioxidant power (FRAP) method. The juice was tested qualitatively and quantitatively using the FRAP method. The qualitative test results after adding the FRAP reagent were positive for antioxidant activity because the color changed to blue. In quantitative determination of absorbance, antioxidant activity was measured using the UV-Vis spectrophotometric method. Absorbance was measured at a maximum wavelength of 701 nm using ascorbic acid as a reference solution. The equation obtained is y = 0.0103x + 0.3801 with a value of r = 0.9978. The total antioxidant activity value was calculated based on absorption data. The calculation results for the total antioxidant activity value were 173.68 mgAAE/L sample. Therefore, we can conclude that red fruit can be used as a natural antioxidant.