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Nor Sa'adah
Politeknik Bumi AKPELNI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Earth & Planetary Sciences Energy Engineering Environmental Science Transportation

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Pengaruh Pemberian CO2 terhadap pH Air pada Pertumbuhan Caulerpa racemosa var. uvifera Nor Sa'adah; Sekar Widyaningsih
Jurnal Kelautan Tropis Vol 21, No 1 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.531 KB) | DOI: 10.14710/jkt.v21i1.2460

Abstract

Although in the growing season, seaweed type Caulerpa racemosa var. uvifera sometimes has not be found in the field due to environmental factors. So seaweed mariculture is urgently needed. This research was aimed to study the impact of providing CO2 with different duration in the water media on the growth of C. racemosa var. uvifera. This research was used 5 treatments with 3 repetitions, namely: A (without CO2 as a control), B (providing CO2 for 6 hours per day), C (giving CO2 for 12 minutes per day), D (CO2 delivery, 18 minutes per day) and E (providing CO2 for 24 minutes per day). The observed data in the form of wet weight and water quality that were used as maintenance medium. The results showed that providing CO2 with different duration significantly affected (P <0.01) among treatments. The highest specific growth rate of C. racemosa var.uvifera was in the B (5.270 +0.022)% per day and the lowest in E (1.908 +0.069)% per day. The CO2 contents in treatment B ranged from 95.88 to 108.11 with a pH ranging from 7.0 to 8.4 and CO2 contents in treatment E was 382.87-412.08 with a pH ranging from 6.0 to 8.0. Rumput laut jenis Caulerpa racemosa var. uvifera walaupun sudah musimnya tumbuh kadang tidak ditemukan di perairan, karena kondisi lingkungan sangat mempengaruhi. Hal ini mengakibatkan produksi jenis tanaman tersebut langka, sehingga perlu dilakukan budidaya. Penelitian ini bertujuan untuk mengkaji pemberian CO2 yang berbeda terhadap pH media air pemeliharaan pada pertumbuhan C. racemosa var. uvifera. Penelitian ini menggunakan 5 perlakuan dengan 3 kali pengulangan, yaitu : A (tanpa CO2 sebagai kontrol), B (pemberian CO2 selama 6 menit per hari), C (pemberian CO2 selama 12 menit per hari), D (pemberian CO2,18 menit per hari) dan E (pemberian CO2 selama 24 menit per hari). Data yang diamati berupa berat basah dan kualitas air yang digunakan sebagai media pemeliharaan. Hasil penelitian menunjukkan bahwa pemberian CO2 dengan lama waktu yang berbeda memberikan pengaruh yang sangat nyata (p<0,01) antar perlakuan. laju pertumbuhan spesifik C. racemosa var.uvifera tertinggi pada perlakuan B (5,270+0,022) % per hari dan terendah pada perlakuan E (1,908+0,069) % per hari. CO2 yang terkandung pada perlakuan B berkisar 95,88-103,11 dengan pH berkisar 7,0-8,4 dan CO2 pada perlakuan E sebesar 382,87-412,08 dengan pH berkisar 6,0-8,0.  
Bakteri Simbion Karang Lunak Sinularia sp. sebagai Agen Antibakteri Sekar Widyaningsih; Nor Sa’adah
Jurnal Kelautan Tropis Vol 21, No 1 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.071 KB) | DOI: 10.14710/jkt.v21i1.2454

Abstract

The disease caused by bacterial pathogen infection has become a major problem in the world of health. Therefore, it needs new bioactive compond to resolve that problem. Symbiotic bacteria of softcoral are one promising source. This is because symbiotic bacteria of softcoral have the same bioactive compounds as their host. Symbiotic bacteria were isolated from softcoral Sinularia sp. that collected from Tanjung Gelam Waters, Karimunjawa Jepara. The symbiotic bacteria screened for antibacterial activity against E.coli, Proteus sp., Enterobacter sp. and S. aureus with diffution agar method. One of ten symbiotic bacteria was active against, SNTGA 5, with inhibition zone 9.067 ± 0.305 mm against E coli, 8.533 ± 0.416 mm against S. aureus, 9.067 ± 0.058 mm against Enterobacter sp., dan 9.1± 0.557 mm against Proteus sp. The molecular identification based on partial 16S DNA, that active isolate was closely related to Pseudomonas stutzeri. Penyakit akibat infeksi bakteri patogen telah menjadi masalah utama dalam dunia kesehatan. Oleh karenanya, diperlukan bahan aktif baru guna mengatasi masalah tersebut. Bakteri simbion karang lunak merupakan salah satu sumber yang menjanjikan. Hal ini karena bakteri simbion karang lunak memiliki senyawa bioaktif yang sama dengan inangnya. Bakteri simbion diisolasi dari karang lunak Sinularia sp. yang dikoleksi dari Perairan Tanjung Gelam Karimunjawa Jepara. Bakteri simbion diuji aktivitasnya terhadap E.coli, Proteus sp., Enterobacter sp. dan S. aureus dengan metode difusi agar. Satu dari 10 bakteri simbion memiliki aktivitas antibakteri, SNTGA5, dengan besar zona hambat 9.067 ± 0.305 mm terhadap E coli, 8.533 ± 0.416 mm terhadap S. aureus, 9.067 ± 0.058 mm terhadap Enterobacter sp., dan 9.1± 0.557 mm terhadap Proteus sp.. Identifikasi molekuler dilakukan dengan menggunakan PCR 16S DNA, dimana isolat yang aktif memiliki memiliki kekerabatan terdekat dengan Pseudomonas stutzeri. 
ANALISIS KESUBURAN PERAIRAN BERDASARKAN KELIMPAHAN FITOPLANKTON DI MUARA SUNGAI PORONG, SIDOARJO Devi Setyowardani; Nor Sa’adah; Nirmalasari Idha Wijaya
Jurnal Riset Kelautan Tropis (Journal Of Tropical Marine Research) (J-Tropimar) Vol 3 No 1 (2021): April
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jrkt.v3i1.54

Abstract

The disposal of Lapindo mud which carries nutrients and other dissolved organic matter can cause mass enrichment and death of organisms due to the reduced amount of light entering the waters. The existence of phytoplankton can provide information about the state of the waters. This study aims to determine the fertility of the waters of the Porong River through the abundance of phytoplankton. This research was conducted from October to January 2021. Sampling was carried out at three stations. The plankton sampling method was carried out horizontally and identified using the Sedgwick Rafter Counting Cell (SRCC). Nitrate and phosphate water samples used for analysis were measured by Uv-Vis Spectrophotometry. Data analysis includes phytoplankton density, diversity index, uniformity index, and dominance index. The results showed that the composition of the phytoplankton species found during the observation was dominated by diatom classes (13 genera ) and dinoflagellates (2 genera). The abundance value of phytoplankton is 122.293 – 867.776 cells/m3. The water fertility status in the Porong River Sidoarjo is in the eutrophic category, namely waters with high fertility levels.
PREVALENSI PENYAKIT PADA KARANG KERAS DI PERAIRAN KALEDUPA, TAMAN NASIONAL WAKATOBI Ainul Rahman; Nor Sa'adah; Nirmalasari I. Wijaya; Ardhi Syam Bahroun
Jurnal Riset Kelautan Tropis (Journal Of Tropical Marine Research) (J-Tropimar) Vol 3 No 2 (2021): November
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jrkt.v3i2.42

Abstract

Coral disease is a disturbance to coral health that causes physiological disturbances for coral biota. The emergence of coral disease is characterized by discoloration, damage to skeleton coral biota, to the loss of its tissueThis study aims to identify the type of coral disease and determine its prevalence in Kaledupa Waters, Wakatobi National Park, Southeast Sulawesi. This research was conducted in June 2021 in the Kaledupa Waters of Wakatobi National Park using the Belt Transect method with a size of 3 x 50 m at 3 observation points at a depth of 3-5 meters. Measurement of Oceanographic Physics-Chemistry parameters is carried out directly in the field. Identification of coral diseases was carried out descriptively based on the coral disease handbook and journal references. The results obtained found 4 types of coral disease that infect coral biota in the waters of Kaledupa Island, Wakatobi National Park, namely Black Band Disease (BBD), White Band Disease (WBD), White Pox and Ulcerative White Spot (UWS). As well as the results obtained for the overall prevalence of coral disease in the study site an average of 1.5%.
ANALISIS BAKTERI SIMBION MANGROVE AVICENNIA MARINA SEBAGAI ANTIFOULING Alifia Rizky Novitasari; Nor Sa'adah; Mahmiah
Jurnal Riset Kelautan Tropis (Journal Of Tropical Marine Research) (J-Tropimar) Vol 3 No 2 (2021): November
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jrkt.v3i2.43

Abstract

The accumulation of biofouling on a substrate causes material loss and huge economics in the maintenance of aquaculture systems, ship hulls, seawater pipelines, coastal power plants, and other marine infrastructure. Prevention of the attachment of biofouling organisms using antifouling paints that have been developed and used for a long time. Antifouling paint contains chemical compounds (biocides). The most sensitive organisms to antifouling paint are gastropods and bivalve that cause endocrine disorders, larval disorders and shell malformations. This study aims to obtain antifouling bacteria through the isolation process of bacteria obtained from the bark Avicennia marina mangrove. The method was beginning with isolation of bacteria bark of Avicennia marina mangrove, identification of bacteria, and antifouling activity test. The results obtained from isolation of bacteria, there are 9 bacteria endophytic symbionts of the inner bark and 9 epiphytic bacteria of the outer bark. The inhibition zone test of the endophytic bacteria in the bark showed that there was no antifouling activity.
Bakteri Simbion Akar Mangrove Avicennia Sp. Sebagai Pendegradasi Pewarna Tekstil Nor Sa'adah
Barakuda'45 Vol 2 No 2 (2020): Edisi November
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas 17 Agustus 1945 Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.894 KB) | DOI: 10.47685/barakuda45.v2i2.91

Abstract

Textile dye is a material that has a negative effect on environmental quality, one of the textile dyes is violet crystal. Biodegradation is one of the methods that used in waste treatment and can take advantage of microbial activity to degrade waste. Mangroves are ecosystems that are found along coastal waters in tropical and subtropical areas. Microorganisms such as bacteria are able to live and develop in mangrove roots. This study aims to isolate and identify the bacteria that live on mangrove roots morphologically and are able get degrade textile dyes. This research was conducted using experimental laboratory methods. Samples were isolated from mangrove roots obtained in Wonorejo Surabaya sterilely. The process of isolation and verification of bacterial morphology was carried out at the Microbiology Laboratory of Hang Tuah University, Surabaya. The method of isolation was carried out using the spread method, and the purification of the bacteria was carried out using the streak method. The results obtained 10 bacterial isolates from the results of isolation and morphological examination, while 2 isolates were able to degrade the violet crystal textile dye, called AB.1.2 and AB.1.5 which were indicated by the presence of clear zones.
Isolasi dan Identifikasi Bakteri Pengurai Mikroplastik Polyethylene Terephthalate dari Sedimen Ekosistem Mangrove Pasir Putih Alifia Rizky Novitasari; Woro Hastuti Satyantini; Sapto Andriyono; Nor Sa&#039;adah
Journal of Marine Research Vol 12, No 1 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i1.37503

Abstract

Polyethylene terephthalate (PET) adalah salah satu polimer termoplastik paling banyak tersedia di pasar. PET digunakan untuk produksi botol atau wadah untuk minuman, seperti air, minuman ringan berkarbonasi, minuman jus, dan industri makanan. Polyethylene terephthalate (PET) ditemukan melimpah pada sedimen ekosistem mangrove di Pasir Putih Wonorejo pada musim hujan sebanyak 59%. Studi terbaru telah menunjukkan bahwa bakteri indigenous sedimen mangrove mampu mendegradasi mikroplastik. Sehingga, tujuan penelitian ini adalah menemukan potensi agen biodegradasi mikroplastik dari bakteri indigenous sedimen ekosistem mangrove di Pasir Putih Wonorejo. Bakteri diisolasi pada April 2022 dari sedimen ekosistem mangrove. Metode yang digunakan diawali dengan isolasi bakteri dari sedimen ekosistem mangrove Pasir Putih Wonorejo, kemudian digunakan untuk uji degradasi mikroplastik polyethylene terephthalate dan mengetahui jenis bakteri yang memiliki kemampuan mendegradasi mikroplastik. Hasil isolat bakteri yang didapat sebanyak 16 bakteri. Potensi bakteri yang menjadi agen biodegradasi dengan penurunan berat mikroplastik isolat bakteri SPP.1.1.3. sebesar 8,73%. Identifikasi molekuler dengan cara ekstraksi DNA 16S rRNA menunjukkan jenis Pseudoaltoromonas caenipelagi dengan homologi 99% pada database National Center Biotechnology Information. Hasil ini membuktikan bahwa bakteri indigenous memiliki kemampuan sebagai agen biodegradasi mikroplastik. Polyethylene terephthalate (PET) is one of the most widely available thermoplastic polymers on the market. PET is used for the production of bottles or containers for beverages, such as water, carbonated soft drinks, juices, and the food industry. Polyethylene terephthalate (PET) is found abundantly in mangrove ecosystem sediments in Pasir Putih Wonorejo during the rainy season as much as 59%. Recent studies have shown that indigenous mangrove sediment bacteria are able to degrade microplastics. Thus, the purpose of this study was to find potential agents for biodegradation of microplastics from indigenous bacteria in mangrove ecosystem sediments Pasir Putih Wonorejo. Bacteria were isolated in April 2022 from mangrove ecosystem sediments. The method used begins with The isolation of bacteria from mangrove ecosystems sediments from Pasir Putih Wonorejo, then were used to assay biodegradation of polyethylene terephthalate microplastics and to determine the types of bacteria that has ability to degrade microplastics. The results of isolated bacterial obtained were 16 bacterias. The potential bacteria that becoming agents of biodegradation by decreasing the weight of microplastic isolate SPP.1.1.3. by 8,73%. Molecular identification by extraction of DNA 16S rRNA showed the type of Pseudoaltoromonas caenipelagi with 99% homology in the National Center Biotechnology Information database. These results prove that indigenous bacteria have the ability as microplastic biodegradation agents.
Profil Metabolit Ekstrak Etanol Enhalus acoroides (L.F.) Royle,1839 dari Nusa Tenggara Timur Mahmiah Mahmiah; Nor Sa’adah; Heronima Natalia Sunur; Nani Wijayanti
Journal of Marine Research Vol 12, No 1 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i1.35076

Abstract

Balauring, Nusa Tenggara Timur, merupakan salah satu wilayah yang berada di bagian Timur Indonesia yang terdapat banyak ekosistem lamun salah satu jenisnya adalah Enhalus acoroides. Pengembangan potensi lamun jenis ini oleh masyarakat masih kurang dimanfaatkan hanya sebagai sampah laut di perairan. Secara kemotaksonomi, daun tanaman lamun Enhalus acoroides mengandung senyawa bioaktif yang berpotensi untuk dijadikan alternatif pengobatan. Tujuan penelitian ini untuk mengidentifikasi kandungan senyawa bioaktif daun tanaman lamun Enhalus acoroides dari Balauring Nusa Tenggara Timur. Adapun metode ekstraksi maserasi dengan pelarut etanol 96%, parameter standardisasi non spesifik, skrining fitokimia, dan analisis FTIR (Fourier Transform InfraRed) dan GC/MS. Hasil penelitian menunjukkan Enhalus acoroides dari keluarga Hydrocharitaceae memiliki nilai kadar air, susut pengeringan dan kadar abu berturut-turut sebesar 6%, 12,9280% dan 14,7173%. Hasil identifikasi senyawa metabolit sekunder ekstrak etanol 96% Enhalus acoroides didapatkan gugus ikatan senyawa seperti gugus -OH atau -NH (ⱱ 3329.958 dan 3252.176 cm-1), -CH (ⱱ 2921.204 dan 2851.486 cm-1), -C=O (ⱱ 1641.169 cm-1), -C=C (ⱱ 1517.243 cm-1). Hasil GC/MS menunjukkan adanya 9 senyawa yang didominasi golongan alkaloid, flavonoid, terpenoid, dan polifenol.Balauring, East Nusa Tenggara, is one of the areas in eastern Indonesia with many seagrass ecosystems, one of which is Enhalus acoroides. The community's potential development of this type of seagrass is still underutilized, serving only as marine debris in the waters. According to chemotaxonomic analysis, the leaves of the seagrass plant Enhalus acoroides contain bioactive compounds that have the potential to be used as alternative treatments. Research objective: to identify the content of bioactive compounds in the leaves of the seagrass plant Enhalus acoroides from Balauring, East Nusa Tenggara. Methods: maceration extraction method with 96% ethanol solvent, non-specific standardization parameters, phytochemical screening, and FTIR (Fourier Transform InfraRed) and GC/MS analysis. Results: Enhalus acoroides, a member of the Hydrocharitaceae family, had moisture content, drying loss, and ash content values of 6%, 12.9280%, and 14.7173%, respectively. Secondary metabolites of Enhalus acoroides 96 percent ethanol extract were identified as-OH or-NH groups (3329,958 and 3252.176 cm-1),-CH (2921,204 and 2851,486 cm-1),-C=O (1641,169 cm-1), and-C=C (1517,243 cm-1). The GC/MS results revealed 9 compounds that were dominated by alkaloids, flavonoids, terpenoids, and polyphenols.
Akumulasi Logam Berat Cu Dan Hg pada Mangrove Rhizopora mucronata di Pantai Timur Surabaya (Pamurbaya) Mahmiah Mahmiah; Nor Sa&#039;adah; Engki Andri Kisnarti; Fachrani Vebranti Millenia
Jurnal Kelautan Nasional Vol 18, No 1 (2023): APRIL
Publisher : Pusat Riset Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jkn.v18i1.11740

Abstract

Cemaran logam berat seperti Cu dan Hg di Pantai Timur Surabaya (Pamurbaya) dikhawatirkan memberikan dampak terhadap kualitas perairan. Mangrove yang tumbuh di Pamurbaya dapat digunakan sebagai indikator cemaran logam berat. Kemampuan mangrove dalam mengakumulasi logam berat ditunjukkan melalui nilai bioakumulasi (BAF), biokonsentrasi (BCF), dan translokasi faktor (TF). Salah satu mangrove yang terdapat di Pamurbaya adalah R.mucronata. Mangrove inimemiliki akar penyangga, selain fungsinya untuk membantu tegaknya pohon, akar jenis ini juga dapat menahan dan memantapkan sedimen tanah, sehingga mencegahtersebarnya bahan pencemar ke area yang lebih luas. Penelitian ini bertujuan untuk mengidentifikasi bioakumulasi cemaran logam berat Cu dan Hg pada mangrove R. mucronata, air, dan sedimen di  Pantai Timur Surabaya. Sampel penelitian diambil pada tiga lokasi melalui purposive sampling. Analisis logam berat Cu dan Hg  menggunakan metode Atomic Absorption Spectroscopy (AAS) dan parameter fisika-kimia oseanografi diukur secara in situ dengan pengulangan sebanyak 3 kali. Hasil penelitian menunjukkan kadar logam berat Cu pada air lebih tinggi dibandingkan sedimen berturut-turut Stasiun 1>Stasiun 2>Stasiun 3.  Hasil cemaran logam Hg pada mangrove R. mucronata, air dan sedimen menunjukkan nilai di bawah baku mutu dengan kisaran  0,0004< Hg<0,0006 ppm. Berdasarkan perhitungan nilai bioconcentration factor (BCF) dan translocation factor (TF), diketahui bahwa kemampuan mangrove R.mucronata sebagai tumbuhan excluder dan fitoekstraksi sehingga mampu mengurangi polutan logam berat dari lingkungan melalui proses penyerapan melalui akar dan translokasi menuju daun.
Dampak Sampah Anorganik terhadap Vegetasi Mangrove Tingkat Semai di Ekosistem Mangrove Wonorejo Surabaya Sherlin Fatwa Fatmalah; Nor Sa'adah; Nirmalasari Idha Wijaya
Jurnal Riset Kelautan Tropis (Journal Of Tropical Marine Research) (J-Tropimar) Vol 4 No 2 (2022): November
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jrkt.v4i2.57

Abstract

The Wonorejo mangrove area is a mangrove forest area that has innovated into an ecotourism area, resulting in a lot of garbage found in the mangrove forest area from visitors. Inorganic waste is waste produced from non-biological materials in the form of synthetic products or the result of technological processes for managing mining materials or natural resources and cannot be decomposed by nature, for example plastic. Mangrove vegetation at the seedling level is rejuvenation from sprouts to a height of 1.5 m. The objectives of the study were to identify the density of inorganic waste in the mangrove vegetation at the seedling level, to analyze the density of the vegetation at the seedling level, to analyze the impact of inorganic waste on the growth of the vegetation at the seedling level. The method used to measure the density of mangroves and waste density using Line Plot Transects. The most waste found was 46 plastic bottles, and other inorganic waste found was styrofoam, and the density of seedlings was 2200 seedlings/ha. The relationship between waste density and seedling density showed (R²) of 0.8399 which indicated that the negative correlation was the more waste, the less seedlings. The impact caused by inorganic waste can threaten mangrove plants, especially at the seedling level and the life of the biota in the ecosystem.
Co-Authors Ainul Rahman Alifia Rizky Novitasari Alifia Rizky Novitasari Alifia Rizky Novitasari Amir Yarkhasy Yuliardi Ardhi Syam Bahroun Devi Setyowardani Engki Andri Kisnarti Fachrani Vebranti Millenia Hermalita Azkiyah Sakhi Heronima Natalia Sunur Ika Pibria Ningrum Mahmiah Maisaroh, Dian Sari Meisel Kristian Dui Misbakhul Munir Nani Wijayanti Nirmalasari I. Wijaya Nirmalasari Idha Wijaya Rafid Gifa Muhammad Raka Sapto Andriyono Sekar Widyaningsih Sherlin Fatwa Fatmalah Spanton, Perdana Ixbal Syamsul Arifin Urip Prayogi Woro Hastuti Satyantini Yahya Abdillah Al Hanif Yunita Vernandha