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All Journal At-Taqaddum THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Biologi Tropis Conference on Innovation and Application of Science and Technology (CIASTECH) Klinikal Sains (Jurnal Analis Kesehatan) JURNAL KESEHATAN PERINTIS Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Jurnal Peduli Masyarakat Indonesian Journal of Global Health research International Journal of Health Science and Technology
Muhammad Taufiq Qurrohman, Muhammad Taufiq
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Detection fimH Gene in The Urine of UTI Patients at The Puskesmas Banyuanyar, as a Marker for The Presence of Escherichia coli Sawitri, Debora; Dewangga, Vector Stephen; Qurrohman, Muhammad Taufiq
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6852

Abstract

Urinary Tract Infection (UTI) is a bacterial infection characterized by a urine culture showing bacteriuria with bacterial counts exceeding 100,000 per milliliter of urine. Polymerase Chain Reaction (PCR) is a method that utilizes in-vitro enzymatic reactions to amplify specific DNA targets. This process synthesizes new DNA strands complementary to the target sequences using enzymes and oligonucleotide primers within a thermocycler. The fimH gene is among the most important of the UPEC strains and is associated with severe urinary tract infections. The aim of this study was to identify the presence of the fimH gene in urine samples from UTI patients at the Puskesmas Banyuanyar in Surakarta using Polymerase Chain Reaction (PCR). The research employed the Quota Sampling Technique, collecting a total of five urine samples from individuals diagnosed with urinary tract infections. The results of the control isolates using pure Escherichia coli isolates showed that the fimH gene PCR amplified control with annealing temperature of 52°C. The analysis of clinical isolates of Escherichia coli from the urine of urinary tract infection patients revealed that the fimH gene was PCR amplified in all five samples.
Detection of Candida albicans in Urine Patients of Diabetes Melitus using Polymerase Chain Reaction (PCR) Method Exzora, Helmalia Bilqis; Qurrohman, Muhammad Taufiq
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7173

Abstract

Candida albicans is a dimorphic fungus that can grow in culture as a blastopore or as pseudohyphae. Laboratory diagnosis of the Candida albincans fungus can be carried out molecularly, which aims to identify DNA in the fungus being examined and carried out by going through the initial molecular stage, namely DNA isolation. The purpose of this study was to determine whether the fungus Candida albicans was present in urine isolate samples from diabetes mellitus sufferers at the Wonosari 1 Klaten Community Health Center. The research method used is descriptive. The samples used were urine samples from diabetes mellitus sufferers at the Wonosari 1 Community Health Center. Based on the research results, data analysis and discussion in this study, it can be concluded that the Candida albicans fungus can be detected in the ITS2 region at a product length of 107bp using the Polymerase Chain Reaction (PCR) method.
Detection of mecA Gene As a Marker for Staphylococcus aureus Types of Methicillin Resistant Staphylococcus aureus (MRSA) Using PCR Technique Sari, Monica Ayu Retno; Qurrohman, Muhammad Taufiq; Dewangga, Vector Stephen
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7539

Abstract

Staphylococcus aureus is a Gram-positive bacterium responsible for various infections, showing strong resistance to β-lactam antibiotics due to the mecA gene, which encodes Penicillin-Binding Protein 2A (PBP2A). This resistance contributes to the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA), posing significant challenges in clinical settings. Detecting the mecA gene is crucial for identifying MRSA and guiding appropriate treatments. This study aimed to detect the mecA gene in clinical pus samples from patients with MRSA infections in hospitals in Surakarta. A qualitative descriptive design was used. DNA was isolated from 12 clinical pus samples, and the mecA gene was detected using Polymerase Chain Reaction (PCR). Gel electrophoresis visualized the amplified DNA bands, and results were analyzed based on comparison with positive and negative controls and a DNA ladder. The results showed positive amplification of the mecA gene in all clinical samples, with clear DNA bands at 571 base pairs. One sample (SP1) and the positive control exhibited weaker bands, but the presence of the mecA gene was confirmed in all cases. The study concluded that all clinical samples were positive for the mecA gene, indicating the presence of MRSA. PCR proved to be an effective tool for detecting the mecA gene in clinical samples and is valuable for identifying MRSA in healthcare settings.
Relationship Between Salivary pH and Candida albicans Growth in Elderly with Diabetes Mellitus Putri, Alya Ernita; Qurrohman, Muhammad Taufiq
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10389

Abstract

Candida albicans is the most dominant Candida species found in the oral cavity, this fungus will turn into a pathogen if there is excessive growth on the oral mucosa which is usually called oral candidiasis. Elderly people with diabetes mellitus have a higher vulnerability due to a weakened immune system that supports the colonization of pathogenic microorganisms. Low pH conditions in saliva can facilitate the growth of Candida albicans fungi, thereby causing pathogenic microorganisms to multiply more rapidly. The purpose of this study was to determine the relationship between salivary acid pH and the growth of Candida albicans fungi in elderly people with diabetes mellitus. The study used an analytical observational design, the sample used in this study were 24 elderly patient respondents with diabetes mellitus. From the results of the study, it can be concluded that there is a significant relationship between salivary acid pH and the growth of Candida albicans fungi in elderly people with diabetes mellitus, which indicates that salivary pH can affect fungal colonization in the oral cavity. Acidic salivary pH conditions have the potential to significantly increase the number of Candida albicans colonies, thereby increasing the risk of oral candidiasis in people with diabetes mellitus.
Edukasi Pemanfaatan Aloe vera Sebagai Bahan Alternatif Untuk Mengurangi Kandidiasis Qurrohman, Muhammad Taufiq; Sitohang, Dahlan; Rizki, Andita Ainal; Shaleha, Annisa Azizah; Rasid, Bryan Aditya Nur; Sanita, Hanifah Velda; Annisa, Mariana Suryanti Nur; Maharani, Raras; Tambunan, Yohana Nathalia
Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Vol 9, No 4 (2025): Jurnal Pelayanan dan Pengabdian Masyarakat (PAMAS)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM Universitas Respati Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/pamas.v9i4.3668

Abstract

Candidiasis is an opportunistic fungal infection that frequently affects the skin, particularly among individuals with suboptimal personal hygiene and those living in humid environments. One natural substance that has the potential to inhibit the growth of Candida albicans is Aloe vera, which contains saponin and lupeol with antifungal activity through the mechanism of disrupting fungal cell membrane integrity. Based on this background, a community service program was conducted to enhance the knowledge and skills of residents in utilizing Aloe vera as a natural ingredient for the prevention of candidiasis. The activity took place in Bentakan Village, Baki District, Sukoharjo Regency, involving 18 women of productive age as participants. The method included a pre-test, interactive lecture, demonstration of Aloe vera soap production, post-test, and evaluation. The average pre-test score of 54.44 increased to 77.77 in the post-test, with the Wilcoxon test showing p < 0.05. This finding indicates a significant improvement in participants’ understanding of candidiasis prevention and promotes positive changes in personal hygiene behavior. The sustainability of this program is expected to be supported through follow-up training and the establishment of community empowerment groups as small-scale producers of herbal soap with economic value.
Identification of Candida Sp. Fungi in the Oral Cavity of Active Smokers using the Swab Method Putra, Yoga Pratama; Qurrohman, Muhammad Taufiq
Indonesian Journal of Global Health Research Vol 6 No S4 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6iS4.4150

Abstract

Active smoker is anyone who directly smokes cigarettes from a burning cigarette. The oral mucosa is the part that can be damaged by smoking. Candida infections usually develop on mucous membranes such as the mouth and are responsible for the appearance of disorders such as candidiasis in the oral cavity.Which has the potential to become fungal. Candida is a species that is often found in the oral cavity. This research aims to determine the presence of Candida.sp fungus in the oral cavity in Petoran village RT 02/RW09 Jebres Surakarta. This research was carried out in November 2023 - June 2024 using descriptive observational research with kuota sampling and 20 samples were taken of the Population of active smokers in Petoran village RT 02/09 Jeberas Surakarta. Samples were taken using the oral mucosa swab method, then cultured on PDA media and observed macroscopically and microscopically with an objective lens magnification of 40x. The data in this study were analyzed using a distribution approach to examine the spread of Candida sp. fungal infections among active smokers in Kampung Petoran. Out of the 20 samples collected, the identification results showed that 14 samples, or 70%, were infected with Candida sp., while the remaining 6 samples, or 30%, tested negative. This distribution of infections indicates that the majority of active smokers in the village were exposed to Candida sp. This analysis provides an initial overview of the prevalence of Candida sp. fungus among active smokers in the area.
Comparison of Candida albicans Colonies in Saliva of Elderly with and Without Diabetes Tanaya, Angabaya Dera Sumba; Qurrohman, Muhammad Taufiq
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10383

Abstract

Diabetes mellitus is a chronic metabolic disorder that not only affects systemic organs but also influences oral health by increasing susceptibility to opportunistic infections such as oral candidiasis caused by Candida albicans. This condition is more pronounced among the elderly, whose immune functions naturally decline with age. This study aimed to compare the number of Candida albicans colonies in the saliva of elderly individuals with and without diabetes mellitus. An observational analytic study with a comparative cross-sectional design was conducted involving 32 elderly participants, consisting of 16 individuals with diabetes and 16 without. Saliva samples were collected using the passive drooling technique and cultured on CHROMagar-Candida medium for 48 hours at 37°C. The colony counts were analyzed using the Shapiro-Wilk, Levene, and Mann-Whitney U tests. The results showed that the elderly with diabetes mellitus had a significantly higher number of Candida albicans colonies compared to those without diabetes (p < 0.05). Diabetes mellitus increases the colonization of Candida albicans in the oral cavity of the elderly, likely due to hyperglycemia and age-related immune decline. Maintaining stable blood glucose levels and practicing good oral hygiene are essential preventive measures to reduce the risk of opportunistic fungal infections in this population.
Co-Authors Annisa, Mariana Suryanti Nur Ariyanti Ariyanti Cahyanti, Eva Dewangga, Vector Stephen Dewangga, Vector Stephen Dhiya, Salsabila Shafa Exzora, Helmalia Bilqis Fitri, Syifa Aurelya Hastuti, Novita Dwi Khasanah, Novita Uswatun Maharani, Raras Muhammad Thoriq Aulia Khamim Murwani, Antik Ningrum, Anik Puspita Noviana Dewi Priyandari, Damar Ajeng Pujianti, Yunita Putri Putra, Muhammad Andriyansyah Sanky Putri, Adila Purnama Putri, Alya Ernita Rahmawati, Desinta Ramadhan, Intan Nur Rasid, Bryan Aditya Nur Renatasya Silviana Herningtyas Rizki, Andita Ainal Sanita, Hanifah Velda Sari, Monica Ayu Retno Sawitri, Debora Sayekti, Fitria Diniah Janah Shaleha, Annisa Azizah Sitohang, Dahlan Srikandini, Chris Tambunan, Yohana Nathalia Tanaya, Angabaya Dera Sumba Wijayanto, Mustofa Tri Yasinta, Tia Yoga Pratama Putra