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All Journal CHEMISTRY PROGRESS e-Journal Pustaka Kesehatan STOMATOGNATIC- Jurnal Kedokteran Gigi Al-Kimia Jurnal Kimia Terapan Indonesia Eksakta : Berkala Ilmiah Bidang MIPA Dedication : Jurnal Pengabdian Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Indonesian Community Journal Jurnal ABDI MERCUSUAR Dedication : Jurnal Pengabdian Masyarakat
Dwi Koko Pratoko
Fakultas Farmasi Universitas Jember
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : e-Journal Pustaka Kesehatan

 1 
Pengembangan Sensor Kloramfenikol Berbasis Bovine Serum Albumin menggunakan Spektrofotometri UV (The Development of Chloramphenicol Sensor Based on Bovine Serum Albumin using Spectrophotometry UV) Sarah Aisha; Bambang Kuswandi; Dwi Koko Pratoko
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.6609

Abstract

Misuse of antibiotics has been discovered in shrimp exported from Indonesia, one of them is chloramphenicol, therefore it is necessary to develop methods for chloramphenicol detection. In this study, the chemical sensor for chloramphenicol detection has been developed. The sensor developed by immobilized bovine serum albumin (BSA) onto cellulose acetate membrane. The sensor has linear range of 6-16 μg/ml chloramphenicol with r 0.999 and limit of detection (LOD)-limit of quantification (LOQ) value of 0.74-2.21 μg/ml. The RSD value of repeatability and intermediet precision were 2.41% and 2.31%, where the recovery was 100.29%. Three of the five samples of tiger shrimp on the market containing chloramphenicol at level of 1.03; 2.48; and 3 μg/ml. The result also shown in good agreement with conventional spectrophotometry UV-Vis method. Keywords: shrimp, chloramphenicol, BSA, cellulose acetate
Sintesis dan Uji Aktivitas Antibakteri Senyawa N-fenil-4-klorobenzamida Elok Dea Orens Ubung Wisnu; Indah Purnama Sary; Dwi Koko Pratoko
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.7569

Abstract

N-phenylbenzamide is benzamide derivatives, which is potential as an antibacterial agent. N-phenyl-4-chlorobenzamide is N-phenylbenzamide derivative that substitued by chloro to the para position and it was expected to enhance the antibacterial activity. N-phenyl-4-chlorobenzamide was synthesized by reacting 1,3-diphenyilthiourea and 4-chlorobenzoil chloride. This compound has been purified and provided 53% of product with crystal shape, white color, and melting point of 195-197 oC. The purification of this compound was confirmed by TLC and the structure was identified by 1H-NMR, 13C-NMR, and FTIR spectroscopy. This compound was tested for its activity against gram positive bacteria Staphylococcus aureus and gram negative bacteria Pseudomonas aeruginosa, evaluated by well diffusion method and the result showed no activity against both Staphylococcus aureus and Pseudomonas aeruginosa. Keywords: N-phenyl-4-chlorobenzamide, synthesis, antibacterial activity
Sintesis dan Uji Aktivitas Antibakteri Senyawa N-Fenil-4-Bromobenzamida (Synthesis and Antibacterial Activity Assay of N-Phenyl-4-Bromobenzamide) Nazilatul Maghfiroh; Indah Purnama Sary; Dwi Koko Pratoko
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i2.5868

Abstract

Infectious diseases are contagious disease caused by pathogenic microorganisms and can be spread directly or indirectly from one person to another. The aim of this research was to synthesized N-phenyl-4-bromobenzamide as a new antibacterial agent. Thereaction was carried out by reacting 1,3-diphenylthiourea with 4-bromobenzoyl chloride by nucleophilic substitution. The purification of this compound was confirmed by TLC and the structure was identified by UV, IR, 1H-NMR and 13C-NMR. The newly synthesized compound was screened for its antibacterial activity against gram-positive and gram-negative bacteria, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 27853 using well diffusion method. The concentration of the test solution used were 31.25; 62.5; 125; 250; 500; 1,000; 2,000; 4,000; 8,000; and 10,000 μg/ml with levofloxacin as positive control. The synthesized product did not show any antibacterial activity which characterized by the absence of inhibition zone.Keywords: 1,3-diphenylthiourea, N-phenyl-4-bromobenzamide, nucleophilic substitution, antibacterial activity.
Pengembangan Bromfenol Biru dan Bromtimol Biru pada Label Pintar Sensor Kematangan Buah Naga Merah (Hylocereus polyrhizus) (The Development of Bromophenol Blue and Bromothymol Blue on Ripeness Sensor Smart Label on Red Dragon Fruit (Hylocereus polyrhizu Raden Ayu Rifqa Zainatul Hurriyah; Bambang Kuswandi; Dwi Koko Pratoko
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i3.5890

Abstract

The novel on-package color indicator label has been fabricated based on bromophenol blue and mixture between bromophenol blue and bromothymol blue, and tests have been conducted to assess the ripeness of red dragon fruit (Hylocereus polyrhizus). Bromophenol blue (BPB) and mixture between bromophenol blue and bromothymol blue (BTB) were immobilized onto acetate cellulose membrane via adsorption method. The BPB and mixture between BPB and BTB/acetate cellulose membrane as color indicator work based on pH decrease as the volatile organic compounds (e.g. acetic acid). They produced gradually in the package headspace during developing of red dragon fruit. Subsequently, the color of the indicator will change from blue to green for over-ripe indication, which can be visible visually. The results showed that the color indicator could be used to determine the state of ripeness of the red dragon at ambient condition (25±2oC) and chiller condition (4±2oC). The color change of the indicators reflects the pH of headspace of the red dragon packaging. Furthermore, it also in similar trends to the change of several parameters (soluble solids content, pH, texture, sensory evaluation and weight loss) that normally used to characterize the ripeness of red dragon, therefore the indicator can be used for real time visual monitoring of ripeness state of packaged red dragon fruit. Keywords: ripeness indicator, BPB, BTB, red dragon fruit, acetate cellulose membrane
Pengembangan Sensor Kloramfenikol Berbasis Imobilisasi Bovine Serum Albumin (BSA) pada Selulosa Asetat dengan Metode Spektroflorometri (The Development of Chloramphenicol Sensor Based on Bovine Serum Albumin (BSA) Immobilization on Cellulose Acetate using Spectrofluorometry Method) Dhany Alghifari; Bambang Kuswandi; Dwi Koko Pratoko
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i1.3947

Abstract

The chloramphenicol usage in shrimp cultivation is an example of antibiotics abuse that have adverse effects for health. According to Indonesian Drug and Food Agency (BPOM) survey, it showed that from 14 samples of shrimp test, entirely contain residues of chloramphenicol. In this context, the sensor has been developed base on cellulose acetate membrane immobilized with bovine serum albumin (BSA). The chloramphenicol sensor has a linearity range of 2-12 μg/ml and the r value of -0.997. The value of limit of detection is 0.157 μg/ml and limit of quantification is 0.472 μg/ml. The RSD value of repeatability is 1.542 % and the value of intermediate precision is 1.058%. The average value of recovery as the parameter an accuracy test of 95.338 ± 0.636% and this sensor has a good selectivity tests to erythromycin, pellets and shrimp meat. The result also shown in good agreement with the conventional spectrofluorometry method Keywords: shrimp, chloramphenicol, BSA, sensor, spectrofluorometry
Pengembangan Biosensor Berbasis Plastik Zona Mikro untuk Skrining Aktivitas Antidiabetes pada Ekstrak Tanaman Obat Indri Firma Wati; Bambang Kuswandi; Dwi Koko Pratoko
Pustaka Kesehatan Vol 9 No 1 (2021): Volume 9 No.1, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i1.20423

Abstract

Research on the diabetes mellitus (DM) treatment has recently been carried out, especially on discovering antidiabetic agents derived from plants. This study aims to determine the fabrication of microzone plastic-based biosensors, determine the optimal sensor conditions, analysis characteristics, and compare the biosensor with the UV-Vis spectrophotometric method. The results showed that the response time was 15 minutes, the linear range was 500-40000 µg / mL (R = 0.9989). The limit of detection (LOD) was 1109.6 µg / mL, and the limit of quantification (LOQ) was 3698.8 µg / mL. Biosensor filled up the precision parameters with an RSD value of less than 3.7% and an accuracy with recovery in the range of 95-105%. The biosensor is stable in storage at 25 ° C for 270 minutes and at chiller temperature for three days. The antidiabetic activity of the biosensor was compared with the UV-Vis spectrophotometer using the Independent Sample T-test and showed insignificant differences between the two methods.
Pengembangan Label Pintar Pemantau Kematangan Buah Pepaya Potong Berbasis Indikator pH Methyl Red Dan Bromocresol Purple Faizah oktaviana; Nia Kristinigrum; Dwi Koko Pratoko
Pustaka Kesehatan Vol 10 No 3 (2022): Volume 10 No.3, 2022
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v10i3.16613

Abstract

Papaya fruit is generally marketed in a condition not yet ripe until past ripe. During the ripening process, the fruit undergoes changes such as increased sugar levels, changes in texture, changes in color on the skin and flesh of the fruit, changes in the rate of CO2 and ethylene production. Papaya fruit is declared rotten if it has a pH below 5.2. Methyl red (MR) indicator changes from red to yellow in the pH range (4.4 - 6.2) and bromocresol purple (BCP) has a pH of 5.2 (yellow) and a pH of 6.8 (purple). Papaya fruit stored at room temperature began to show rot on the 4th day accompanied by a change in the color of the smart bromocresol purple label to yellow, methyl red to red decreased in weight loss of 6.5332%, pH 4.85, hardness of 69.833 g / mm, and total dissolved solids 9.88% brik. At the temperature of the papaya fruit chiller stored for 9 days has shown foul on the 7th day with the same smart label color as room temperature, a decrease in weight loss of 6.4645%, pH 4.95, hardness of 83g / mm, and total dissolved solids 9.67% brik. The faster the color change of the smart label, the lower the quality of papaya on the packaging so that the fruit is not suitable for consumption.
Penggunaan Indikator Film Edible dari Antosianin Ubi ungu untuk Monitoring Kesegaran Jamur Tiram Putih Seftyani, Mita; Pratoko, Dwi Koko; Kuswandi, Bambang
Pustaka Kesehatan Vol 11 No 3 (2023): Volume 11 No.3, 2023
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v11i3.11938

Abstract

Agriculture products as foodstuffs are needed by many people. One of the processed agriculture products that people like is white oyster mushroom. This was followed by requirements for the quality of white oyster mushroom products, so the analytical tool was needed to determine the freshness of white oyster mushrooms easily and practically. This study aimed to develop an edible freshness sensor based on sweet potato anthocyanin (Ipomoea batatas L.) with an edible membrane mixed between chitosan and corn starch. The edible freshness sensor can be applied to determine the freshness level of white oyster mushrooms with various parameters. The evaluation of the freshness of white oyster mushrooms included shrinkage weight test, pH value, texture value, and sensory evaluation. In determining the value of mean green, the color change of an edible freshness sensor was observed visually and measured using the ImageJ program. The results showed that a change in the color of the freshness sensor is dark purple when the white oyster mushroom is fresh, light purple when the white oyster mushroom is still fresh, and green when the white oyster mushroom is no longer fresh. The value of mean green from the edible freshness sensor increase with decreasing the freshness level of white oyster mushroom.
Co-Authors Adelia A Devi Devi Antonius N. W. Pratama Ari Satia Nugraha Asia Asia Bambang Kuswandi Dhany Alghifari Dian Agung Pangaribowo Dian Agung Pangaribowo Dina Trianggaluh Fauziah Dyan Wigati Edi Susanto Elok Dea Orens Ubung Wisnu Faizah oktaviana Fifteen Aprila Fajrin Gani Asri Muharam Ika Norcahyanti Indah Purnama Sary Indah Purnama Sary Indri Firma Wati Iski Weni Pebrianti lindawati setyaningrum Lindawati Setyaningrum Muhammad Zainul Arifin Nazilatul Maghfiroh Nia Kristinigrum Nia Kristiningrum Raden Ayu Rifqa Zainatul Hurriyah Rahmadania Affelia Dianto Rahmadaniah Affelia Dianto Regol S Raudiah Raudiah Saeful Akhmad Tauladani Sarah Aisha Seftyani, Mita Shinta Mayasari Wanda Tri Agustin Wanda Tri Agustin Wardhani, Firdha Aprillia Wilda Nur Rohmatillah