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D. Elysa Putri Mambang
Program Studi Farmasi, Fakultas Farmasi, Universitas Muslim Nusantara Al-Washliyah, Medan, Sumatera Utara, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Deteksi cemaran Staphylococcus aureus pada ayam krispy lokal di sekitar salah satu universitas kota Medan Yayuk Putri Rahayu; D. Elysa Putri Mambang; Haris Munandar Nasution; Adelia Ramadani
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.222

Abstract

Chicken meat is more in demand by consumers because it is easy to process, can be accepted by all levels of society and has an economical price. Food poisoning can be caused by enterotoxin contamination caused by Staphyococcus aureus. Management of chicken meat that is not up to standard and unhygienic can affect human health. The purpose of this study was to identify the presence of pathogenic bacteria S. aureus in crispy fried chicken meat from roadside carts and local fast food at one of Medan city University and to analyze the contamination value of S. aureus bacteria whether it meets the Indonesian National Standard (SNI) < 1X102 CFU/ml. The research method is employed to detect the presence of S. aureus in samples using both Baird Parker Agar Base (BPA) and Egg Yolk media simultaneously. Quantification of S. aureus contamination is carried out through the application of the Total Plate Count (TPC) method. Findings from two samples of local fried chicken, namely samples A and B, indicate a positive result for the presence of S. aureus, with contamination levels exceeding the threshold of 1 x 102 CFU/ml. This leads to non-compliance with the established SNI standards. Conversely, in the case of local crispy fried chicken samples C and D, the results indicate a negative presence of the S. aureus pathogenic bacteria, with contamination levels not exceeding the threshold of 1 x 102 CFU/ml. Therefore, these results comply with the stipulated SNI standards.
Sediaan krim ekstrak jeruk nipis (Citrus aurantifolia S.) Sebagai Obat luka sayat pada mencit jantan (Mus musculus) Madani Sirait; Gabena Indrayani Dalimunthe; Minda Sari Lubis; D. Elysa Putri Mambang
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.235

Abstract

Indonesia is a country that is rich in plant diversity. One of them is lime, which is used as a traditional wound medicine. Lime contains essential oil compounds, flavonoids, saponins, tannins, alkaloids, citric acid, amino acids, vitamin B1, and vitamin C. Based on previous research, flavonoids, tannins, and saponins can heal wounds. Making simplicia using an oven at 50oC  and characterizing simplicia Extraction using the maceration method with 96% ethanol is then concentrated using a rotary evaporator. Orientation on the preparation of the cream was carried out until a good cream texture was found, and a cream preparation test was carried out to comply with the requirements in the literature. The test animals used were 24 male mice, which were divided into six treatment groups. The first group was treated with betadine, the second group was treated with cream without extract, the third group was treated with 2 grams of extract cream, the fourth group was treated with 3 grams of extract cream, the fifth group was treated with 4 grams of extract cream, and the sixth group was untreated. Each group was given a 10-mm wound. Based on the treatment carried out for 14 days, the best formulation was a 4-gram lime extract cream, and the wound healed on day 9, which was the same as the comparison used, namely betadine.
Efektivitas antibakteri ekstrak etanol daun bisbul (Diospyros discolor Willd) terhadap bakteri Propionibacterium acnes Nanda Nadia; D. Elysa Putri Mambang; M. Pandapotan Nasution; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.240

Abstract

The bisbul plant (Dyospiros discolor Willd) is a plant whose fruit is often consumed by the public. Bisbul leaves are known to have antibacterial and antifungal activity. The purpose of this study was to determine whether the ethanol extract of bisbul leaves (Diospyros discolor Willd) has antibacterial effectiveness against Propionibacterium acnes bacteria. This research was conducted using experimental methods which included sample collection, macroscopic examination, microscopic examination, simplex preparation, characteristic examination, phytochemical screening, preparation of ethanol extract, and testing of antibacterial effectiveness against Propionibacterium acnes bacteria using the agar diffusion method. The results of the macroscopic examination conducted on the bisbul leaf simplex were brownish-green leaves, flat edges, 5 cm long leaves, 4 cm wide leaves, had a distinctive odor and a slightly bitter taste. The simplex characteristic results obtained water content of 4%, water-soluble essence content of 21%, 23% ethanol-soluble extract content, 4.8% total ash content and 0.3% acid-insoluble ash content. Phytochemical screening of simplex powder and ethanol extract of bisbul leaves showed the presence of alkaloids, tannins, flavonoids, steroids/triterpenoids, saponins and glycosides. The results of the antibacterial effectiveness test of the ethanol extract of bisbul leaves showed that the diameter of the inhibition zone was larger at a concentration of 80%, namely 15.98 mm, compared to concentrations of 70% and 60%, namely 15.43 mm and 14.18 mm. From the results of the study it can be concluded that the ethanol extract of bisbul leaves can provide antibacterial effectiveness against Propionibacterium acnes bacteria.
Prevalensi bakteri Salmonella sp. pada daging ayam potong di pasar tradisional, pasar modern, dan merek terkenal di kota Medan. Lulu Ilma Khoirun Nissa; Yayuk Putri Rahayu; D. Elysa Putri Mambang; Anny Sartika Daulay
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.330

Abstract

Chicken is a source of animal protein for humans. Chicken meat generally contains unsaturated fat; chicken meat also has a compact meat texture and simple protein, so it is easily digested. However, chicken meat can also be contaminated with Salmonella sp. Pathogenic bacteria can cause typhus or typhoid fever, caused by traders' lack of cleanliness and hygiene or their environment. Research was conducted to identify the presence of Salmonella sp. Pathogenic bacteria and analysing the value of Salmonella sp. Pathogenic bacteria contamination that met the Indonesian National Standard (SNI), namely harmful/25g in chicken meat from traditional markets, modern markets, and well-known brands in Medan City, and to determine the prevalence value of Salmonella sp. Bacteria. The method of research uses Total Plate Count (TPC). An identification test is first carried out to calculate and determine the presence of bacteria in a test sample for the existence of Salmonella sp. Pathogenic bacteria and choose the total value of contaminants using a Total Plate Count (TPC), then identify using a gram staining test. These biochemical tests include the indole test, MRVP test, citrate test, urea test, TSIA test, and LIA test. Then, the last one determines the Prevalence value of Salmonella sp. Bacteria. The results showed that the samples of traditional market and modern market chicken meat were positive for the presence of the pathogenic bacteria Salmonella sp. by obtaining Subgenus Salmonella Typhimurium on N2H8 sample code APTL and APTB, N3H15 sample code APTL, APTB, APMI and obtaining Subgenus Salmonella Thypi on N3H15 sample code APMS and the contamination value of the pathogenic bacteria Salmonella sp. Exceeds the threshold and does not meet the Indonesian National Standard (SNI), namely negative/25g. The prevalence value obtained by the pathogenic bacteria Salmonella sp. in broiler meat at Traditional Markets, Modern Markets, and well-known brands is 75%, 50%, and 0%, respectively.
Co-Authors Adelia Ramadani Anny Sartika Daulay Gabena Indrayani Dalimunthe Haris Munandar Nasution Lubis, Minda Sari Lulu Ilma Khoirun Nissa M. Pandapotan Nasution Madani Sirait Nanda Nadia Yayuk Putri Rahayu