This study aims to examine how humanistic learning theory is implemented in differentiated learning at MI Azzahro Bojonegoro. Humanistic theory emphasizes the development of individual potential optimally through a personal, empathetic, and learner-centered approach. In the world of education, this approach is in line with the principle of differentiated learning that accommodates the different learning needs of students. By using descriptive qualitative methods, data were obtained through observation, interviews, and documentation. The results show that the application of a humanistic approach in differentiated learning can increase motivation, learning independence, and active participation of students in the learning process.