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Lead (Pb)-Resistant Bacteria Improve Brassica chinensis Biomass and Reduce Pb Concentration in Pb-Contaminated Soil Pertiwi, Beauty Laras Setia; Ustiatik, Reni; Nuraini, Yulia
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86174

Abstract

Applications of inorganic fertilisers  and pesticides frequently increase lead (Pb) content in the soil and food crops. This study aims to isolate Pb-resistant bacteria and test the isolated bacteria in reducing Pb concentration and increasing biomass production of Brassica chinensis on Pb-contaminated soil.  Soil and plant samples were collected from agricultural land in Batu City, East Java, Indonesia. The isolated bacteria were tested for Pb resistance and then characterised  according to 16S rRNA Sequence. A pot trial with a completely randomised  block design consisting of 9 treatments and 3 replications was set to determine the effect of Pb-resistant bacteria inoculation on Pb residue, plant growth, and soil nutrients. The result showed that the isolated Pb-resistant bacteria were Bacillus wiedmannii and Bacillus altitudinis. The bacteria were resistant to Pb up to 10,000 mg/L PbNO3. Inoculation of the bacteria increased B. chinensis growth and biomass production, namely increasing the number of leaves (12%) and dry weight (35%).  Also, the bacteria reduced Pb residue in the soil by up to 88%. Moreover, soil essential nutrients such as total nitrogen, available phosphorus, and exchangeable potassium increased (12%, 73%, and 200%, respectively) after the application of Pb-resistant bacteria. The bacteria have the potential for bioremediation of Pb-contaminated soils on a large scale due to the bacteria prevent Pb uptake by food crops such as B. chinensis by reducing Pb content in the soil, which is good for food safety and environmental sustainability. 
Pengaruh Kombinasi Pupuk Organik dan Anorganik terhadap Sifat Fisik dan Kimia Tanah serta Produksi Padi pada Lahan Kering yang Disawahkan Putra, Rizky Eka; Rayes, Mochtar Lutfi; Kurniawan, Syahrul; Ustiatik, Reni
Agrikultura Vol 35, No 1 (2024): April, 2024
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v35i1.53686

Abstract

Lahan kering yang disawahkan memiliki proses pelumpuran dan penggenangan yang menyebabkan perbedaan kondisi sifat fisik tanah sehingga menurunkan kesuburan tanah, salah satunya yaitu kerusakan struktur tanah. Upaya untuk meningkatkan kesuburan tanah pada lahan tersebut dapat dilakukan dengan meningkatkan C-Organik, salah satunya yaitu aplikasi pupuk organik (asam amino dan asam humat). Aplikasi pupuk organik yang dikombinasikan pupuk anorganik dengan dosis yang tepat juga berpotensi untuk memperoleh produksi tanaman yang optimal. Tujuan penelitian ini yaitu untuk menganalisis perbedaan karakteristik tanah pada lahan kering yang disawahkan serta menganalisis pengaruh pemberian kombinasi pupuk organik dan anorganik terhadap kesuburan tanah dan produksi padi pada lahan kering yang disawahkan. Penelitian dilakukan di Kebun Percobaan Jatimulyo, Universitas Brawijaya. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan 9 perlakuan dan 3 kali ulangan. Parameter yang diukur yaitu pH, C-Organik, kapasitas tukar kation (KTK), Kdd, kejenuhan basa, berat isi, tekstur, jumlah anakan produktif, dan berat gabah kering panen. Hasil penelitian menunjukkan terdapat perubahan sifat fisik dan kimia tanah akibat perubahan pengolahan dari lahan kering menjadi lahan sawah. Aplikasi pupuk organik dan anorganik memberikan pengaruh nyata terhadap sifat kimia seperti pH, C-Organik, N-total, P-tersedia, Kdd dan KTK. Aplikasi pupuk organik dan anorganik juga meningkatkan produksi padi sebesar 39-59% dengan perlakuan dosis terbaik yaitu 75% pupuk dasar anorganik + 100% pupuk organik.
Bio-physico-chemical Soil Characteristic: Intensive Tillage vs. No Tillage Reni Ustiatik; Ayu Putri Ariska; Resa Kharisma Ramadhan; Novryanti Rizqi Aziz; Syamsu Ridzal Indra Hadi; R Muhammad Yusuf Adi Pujo Nugroho; Maydella Vista Putri Rinandy; Muhammad Taufik Hidayat; Wikan Agung Nugroho; Syahrul Kurniawan
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 13, No 4 (2024): December 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i4.1196-1205

Abstract

Soil tillage has both positive and negative impacts on soil quality and crop productivity. Efforts to reduce the negative impacts of intensive soil tillage are urgently needed. This study aims to analyze the impact of intensive soil tillage on soil fertility parameters (pH, available P, organic-C, soil compaction, aggregate stability, and soil biodiversity). This research was conducted in two land uses: agriculture and forest land. The research design was descriptive-explorative through surveys and direct field observations. The sample points were determined using stratified random sampling with 3 replications (24 samples). Parameters analyzed in this study were soil compaction, aggregate stability, soil pH, soil available-P, and soil biodiversity (total microbial, soil meso-and-macrofauna). The results showed that intensive tillage affected the soil microbial population, aggregate stability, pH, and available-P (p<0.05). The negative impact of intensive soil tillage reduced total soil microbes by 59.37%. The soil macro and mesofauna found at the study site were earthworms and mycorrhizae, which had a higher density on non-tillage land, with trees as the main vegetation. This encourages efforts to introduce conservation soil tillage to maintain soil biodiversity before more severe damage occurs. Keywords: Intensive agriculture, Soil degradation, Soil fertility, Soil quality, Soil structure.
Effectiveness of Various Types of Manure and Inorganic Fertilizers on Populations of N-fixing and P-Solubilizing Bacteria and Nutrient Uptake of Maize in Inceptisol Alda Inayah A. Hi. Usman; Reni Ustiatik; Sri Rahayu Utami; Yulia Nuraini
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 13, No 3 (2024): September 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i3.880-889

Abstract

Bacteria are classified as non-symbiotic N-fixing and P-solubilizing play an important role in enhancing soil quality and plant growth. This study aims to analyze various types of manures and inorganic fertilizers on N-fixing and P-solubilizing bacteria population and their relationship with N and P uptake of maize in Inceptisol. The manure used was poultry, goat, and cow manure with doses of 0 t/ha, 10 t/ha, and 20 t/ha, respectively, and inorganic fertilizer NPK Phonska 15-15-15 dose 0 kg/ha; 150 kg/ha (50% of recommendation dose) and 300 kg/ha (100% of recommendation dose). Application of manure (poultry, goat, and cow manure) increased N-fixing and P-solubilizing bacteria population. The application of various types of manure and inorganic fertilizers has a significant impact on N and P uptake. The highest N and P uptake were 29.92 kg/ha and 2.63 kg/ha, respectively. Manure application can reduce inorganic fertilizer dose by up to 50%, increasing plant production efficiency and environmental sustainability. Keywords: Bacteria population, Fertilizer optimization, Inceptisol, Nutrient uptake.
The Role of Cadmium-Resistant Bacterial Application and Compost in Promoting Water Spinach Growth and Reducing Cadmium Uptake Azis, Novrianty Rizqi; Nuraini, Yulia; Ustiatik, Reni; Prayogo, Cahyo
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 14, No 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.417-423

Abstract

The continuous use of chemical fertilizers and pesticides in soil can result in the presence of cadmium (Cd) residues that may interfere with plant growth and pose a risk of uptake by plants. The issue of soil contamination by Cd can be addressed through soil bioremediation, which involves the use of Cd-resistant bacteria and compost. The objective of this study was to analyze the impact of Cd-resistant bacteria and compost application on the growth and Cd uptake of water spinach. The research design was a completely randomized design with seven treatments: (1) control, (2) 5 mL Cd-resistant bacteria consortium, (3) 10 mL Cd-resistant bacteria consortium, (4) 10 tons/ha compost, (5) 20 tons/ha compost, (6) 5 mL Cd-resistant bacteria consortium +10 tons/ha compost, and (7) 10 mL Cd-resistant bacteria consortium +20 tons/ha compost. The results demonstrated that the application of Cd-resistant bacteria and compost did not notably impact the growth of water spinach. However, it did significantly influence the reduction of Cd uptake in water spinach. The application of the Cd-resistant bacterial consortium and compost was effective in reducing the Cd uptake of the water spinach plants in all treatments. The combination of 10 mL of bacterial isolate and 20 tons/ha of compost demonstrated the greatest reduction in Cd uptake by water spinach, reaching 73%. Keywords: Bioremediation, Cadmium-resistan bacteria, Compost, Environmental monitoring, Soil contamination.