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All Journal Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Aceh Journal of Animal Science Jurnal Medik Veteriner JURNAL PENDIDIKAN TAMBUSAI NERSMID: Jurnal Keperwatan dan Kebidanan Journal of Parasite Science Conservative Dentistry Journal Jurnal Kedokteran Hewan

Heni Puspitasari

Institute Of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia

Author-ID : 2761632

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Molecular Review Covid19 from the Pathogenesis and Transmission Aspect Fery Setiawan; Heni Puspitasari; Jenny Sunariani; Ahmad Yudianto
JURNAL KESEHATAN LINGKUNGAN Vol. 12 No. 1si (2020): SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v12i1si.2020.93-103

Introduction: Corona disease-19 virus (COVID-19) spread and caused a pandemic that affected people all over the world. COVID-19 is also called Severe Acute Respiratory Syndrome-Coronavirus Disease (SARS-CoV). Discussion: COVID-19 is a β-coronavirus serotype which is a single strain of RNA virus and was an outbreak in 2002 (SARS-CoV) and 2012 (MERS-CoV). COVID-19 has Open Reading Frames (ORFs) consisting of Spikes (S), Envelopes (E), Membranes (M), and Nucleocapsids (N) with S parts being a glycoprotein that can attach to receptors owned by host cells, the receptors are CD 26, ACE-2, Ezrin, and Cyclophilins with the main receptor being ACE-2 in the lung organs. Process would evoke a host body's immune response consisting of natural and adaptive immune systems, involving the Antigen Presenting Cell (APC) system which consists of two, namely: Major Histocompatibility Complex (MHC) class I and II. APC could also generate adaptive immune system, consists of B and T cells. COVID-19 had the ability to survive in B and T cells, so that cytokine-chemokine secretion continues to be known as cytokine storm that trigger Acute Respiratory Distress Syndrome (ARDS) and death. Conclusion: The recovery prognosis of COVID-19 depended on the detection of COVID-19 patients because it was related to the severity of ARDS, so the earlier it was detected, the greater the chance of recovery.

Blastocystis sp. : Evaluation of polyclonal antibody prepared from crude protein for serological diagnosis using Rabbit serum Septian Hakim Susantoputro; Lucia Tri Suwanti; Rahadju Ernawati; Mufasirin Mufasirin; Setiawan Koesdarto; Wiwiek Tyasningsih; Heni Puspitasari
Aceh Journal of Animal Science Vol 5, No 2 (2020): Desember 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.2.16780

The diagnosis of Blastocystis infection is still based on the clinical sign which is not specific and there is no available serologic test for it. This study aimed to evaluate the polyclonal antibody prepared form crude protein of Blastocystis for the development of the Blastocystis serological test. Crude protein was extracted from the yeast of Blastocystis sp, then inoculated into rabbits to produce the antibody of crude protein. The serum of rabbits would be collected before and after immunization to compare the antibody titer. The profile of crude protein was analyzed using SDS-Page. The rabbit serum was analyzed using ELISA and Western Blot. The SDS-Page result showed bands in 100 kDa, 90 kDa, 70 kDa, 60 kDa, 58 kDa, 50 kDa, 40 kDa, 35 kDa, 30 kDa and 27 kDa. The ELISA assay showed that there was an increase in antibody titer of crude protein after immunization. Western Blot showed that three proteins (30 kDa, 40 kDa and 50 kDa) having immunogenicity characteristic. It is concluded that protein 30 kDa, 40 kDa and 50 kDa prepared from the crude protein of Blastocystis sp. can be used for developing a serologic test for Blastocystis infection. Keywords: Blastocystis sp, Crude Protein, Polyclonal Antibody .

Efektifitas Asam Folat Dalam Mengurangi Efek Samping Terapi Kasus Toxoplasmosis Dilihat Dari Gambaran Histopatologi Sel Otak Fetus Mencit ( Mus Musculus) Yang Diinfeksi Toxoplasma Gondii andi jayawardhana; Fauziah Fitri Hernanto; Heni Puspitasari; Mufasirin; Lucia Tri Suwanti
NERSMID : Jurnal Keperawatan dan Kebidanan Vol. 4 No. 1 (2021): Mei
Publisher :

Show Abstract | Download Original | Original Source | Check in Google Scholar

Period of toddler or early childhood (1-3 years) was the golden period for children development, especially fine motor. Lack of appropriate stimulation at this stage of age can lead to delayed development. Formed of stimulation for toddler was with playing, example playing kinetic sand. It consists various colors, so that will cause interest toddler and they will use their finger skills to shape it. The purpose this study to analyzed effect of playing kinetic sand to the fine motor development of toddler. The design used pre-experiment (one group pre-post test design). The sampling technique was purposive sampling. The location of study was in the Day Care RSUD Dr. Soetomo Surabaya. The period of study started from December 2016 to January 2017. Total population was 60 childrens with a sample size was 21 toddlers. The instrument of study used Activity Program Unit and Denver II. Data analysis used Mc Nemar test. The dependent variable was the fine motor development of toddler and independent variables was playing kinetic sand. The results showed that intervention succeed to improved fine motor development of toddler from 80% (before intervention) to 100% (after intervention). Stimulation of playing kinetic sand was a method to improved the fine motor development of toddlers, so the nurse could socialize it to Day Care and toddler’s parent to used it.

The Use of Artificial Intelligence in New Normal Era Against Pandemic COVID-19 in the Field of Health Services Dian Agustin Wahjuningrum; Fery Setiawan; Setyabudi; Heni Puspitasari; Ahmad Yudianto; Agung Sosiawan; Arif Rahman Nurdianto; Swadheena Patro
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.12-19

Background: Indonesia is currently experiencing Coronavirus Disease-19 (COVID-19) pandemic which affects all sectors of life, therefore Indonesia is starting to prepare for New Normal era, including in health care sector. COVID-19 was previously known as novel Coronavirus Disease-19 (nCoV-19), where World Health Organization (WHO) declared it as a global pandemic in March 2020. New Normal era is an era of coexistence with COVID-19, resulting in new habits that were rarely or never practiced before. One practice that can be applied in health care sector is to use Artificial Intelligence (AI) to treat COVID-19 patients to prevent casualties for health workers. Health care providers are prone to contract aerosol and droplet transmission while treating patients, resulting in cytokine storm, acute respiratory distress syndrome (ARDS), and acute lung injury (ALI), which leads to death. Purpose: This mini review study used to explain the using of artificial intelligence in the new normal era against pandemic COVID-19 in the field of health services. Reviews: This pandemic COVID-19 restricts all of the communication between person to person. It also challenged the health service sector because the health service sector is the first line to combat the COVID-19. It is now popular to use artificial intelligence. This using can be used to help the health service sector in combating the COVID-19 pandemic especially in this new normal era. Conclusion: COVID-19 pandemic affects all aspects of life, especially health care sector, therefore AI use is important to prevent the deaths of health workers because of this pandemic.

PHYLOGENETIC ANALYSIS AND VIRULENCE-CODING GENES CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM LAYING HEN BRAIN WITH TETELO SYMPTOM Safira Iqlima Sarah; Christian Marco Hadi Nugroho; Ryan Septa Kurnia; Dhandy Koesoemo Wardhana; Heni Puspitasari
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

Newcastle disease (ND) is an acute poultry disease caused by Paramyxovirus group. It has characteristic neurological symptoms, called torticollis. The molecular assay to find out the presence of viral genes in the brain can be an option in detecting ND virus infections since it penetrates brain barrier system. The purpose of this study was to identify ND viruses in the brain of chickens with torticollis symptoms, to analyze its phylogenetic and to characterize its virulence genetic code. Samples used were 12 dead chickens with historically had torticollis symptoms, obtained from poultry farms at several areas in West Java and Banten. Chicken brains were prepared for reverse transcriptasepolymerase chain reaction (RT-PCR) test. All positive samples then sequenced to obtain its nucleotide sequences from some of Fusion (F) genes analyzed its phylogenetic by comparing with Indonesian ND isolate virus from GenBank using Mega X software. The results of RT-PCR test showed that only one sample (Virus MSL.03) contained genes of ND virus. Based on homology tests and phylogenetic analysis, the virus belonged to subgenotype VIIh with an identical level of 95.34-95.86% when compared to several isolates from Indonesia. The MSL.03 ND virus has 112RRRKRF117 pattern in F0 indicatest its virulent category.

PRODUCTION AND CHARACTERIZATION OF IMMUNOGLOBULINE YOLK AS ANTI ANTIGEN MEMBRANE TOXOPLASMA GONDII Puspitasari, Heni; Praptiwi, Yuliana; Suwanti, Lucia
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 2 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Toxoplasma gondii is an obligate parasite intracellular which can infected human and other mammalian. Immunoglobulin Y technology offers several advantages better than antibody production in mammals. This research was aimed to get immunoglobulin Y from egg yolk, to prove that antibody against membrane T. gondii antigen can produced from immunoglobulin Y and to know the characterization of immunoglobuline Y according to molecular weight by SDS PAGE and reactivation of antibody antigen by Western Blott. This research devided from many step : passase tachyzoites T. gondii into mice by peritoneal infection, cultivated the tachyzoite from intraperitoneal fluid, preparation of membrane antigen tachyzoite T. gondii, then immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blott. The result of this resarch showed that immunoglobulin Y from egg yolk can produced antibody against protein membrane T. gondii. The result of analyzed profile protein immunoglobuline Y according SDS PAGE has molecular weight 179,8 kDa. Analyzed from Western Blott showed that immunoglobulin Y can recognize antigen epitope of T. gondii on molecular weight 35,7 kDa and 78,8 kDa.

THE IMMUNOSTIMULAN POTENTIAL OF TENGGULUN (PROTIUM JAVANICUM) LEAVES TOWARDS T CELL CD4+ AND IFNÎ³ SECRETION ON PBMC CHICKEN Jayawardhana, Andi; Puspitasari, Heni; Meles, Dewa Ketut; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 3 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

One of the plants with immunostimulant activity is Tenggulun leaves which contain of flavonoid, like terpenoid. The aim of this research is to find the potential of Tenggulun's leaves extract to have the immunostimulant activities. The potential of immunostimulant activity is identified by the increasing the amount of T-cell CD4+ expression and IFNÎ³ secretion. The research method is conducted through cultured chicken PBMC which is infected by ND virus; it is then treated with Tenggulun's leaves extract with immunostimulant. The result of immunocitochemistry examination CD4+ secretion on PBMC cultures shows how tenggulun is significantly different from the control in the secretion CD4+. The 10Î¼g of tenggulun extract can modulate the T cell CD4+ secretion 68.8±0.83. It is significantly different from K (control) (p<0,05) and treatment group K+, P0, and P1. The examination of IFNÎ³ level using ELISA from tenggulun leaves extract of 10Î¼g doses were inoculated after being infected by ND virus contained immonostimulant potential in increasing the secretion of IFNÎ³ 120.91±6.44. It is significantly different from K-, K+, and P1, yet not significantly different from P0. The content of terpenoid can increase IFNÎ³ secretion on the macrofag cells culture and limfosit cells.

Moringa oleifera Leaf Ethanol Extract Inhibits Toxoplasma gondii Tachyzoites Replication Wihanto, Laura; Waworuntu, Gladdy Lysias; Tedyanto, Cecilia Putri; Puspitasari, Heni
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 1 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i1.42672

The various infection routes of Toxoplasma gondii that are close to daily life strongly support the incidence of toxoplasmosis. The emergence of drug-resistant Toxoplasma gondii strains raises future concerns. Moringa leaf ethanol extract has been shown to have several anti-pathogen activities, which could have an anti-Toxoplasma effect. This research was conducted to analyze the anti-Toxoplasma effect of moringa leaf ethanol extract against tachyzoites replication in Toxoplasma gondii and the correlation between extract doses with the number of tachyzoites. Mice were divided into five groups. The negative control group (Group I) received CMC-Na solution. The positive control group (Group II) received spiramycin 100 mg/kg BW. The treatment groups received moringa leaf ethanol extract 250 mg/kg BW (group III), 500 mg/kg BW (group IV), and 1000 mg/kg BW (group V), respectively. Mice were injected with 1 x 105 tachyzoites/0.1 mL/mice intraperitoneally on the first day. Moringa leaf ethanol extract and spiramycin were given orally once daily for three days. The number of tachyzoites in the intraperitoneal fluid was calculated on the fifth day. The results have shown that there were significantly lower differences (P < 0.05) in group IV (P = 0.021) and group V (P = 0.022) compared to group I. There was also a significant negative correlation between the extract doses and the number of tachyzoites (P = 0.000; r = -0.781). Moringa oleifera leaf ethanol extract has an anti-Toxoplasma effect by inhibiting the tachyzoite replication at 500 mg/kg BW and 1000 mg/kg BW.

Strongylids Profile on Horses in East Java Puput, Sesa; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Yunus, Muchammad; Suprihati, Endang; Aksono, Eduardus Bimo; Puspitasari, Heni
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.120-124

Strongylids are a group of worms that infect the digestive tract of horses consisting of Strongylus vulgaris, Strongylus equinus, Strongylus edentates and Cyathostomes. There is no reported prevalence data on Strongylids infection in horses in East Java. Study on Strongylids is urgently needed because this worm is one of the causes of zoonotic diseases. This study aimed to identify Strongylids that infect horses morphologically. Identification of Strongylids in this study using morphological methods. A total of 65 horse faeces in East Java, i.e. in the cities of Surabaya, Malang, Kediri and Pasuruan were examined microscopically after being fixed in 10% formalin. Three microscopically positive samples showed a prevalence of 4,6% with an average size of 60-70µm x 50-60µm and larvae developed inside the worm eggs.

Seroprevalensi Kasus Toxoplasmosis sebagai Infeksi Oportunistik pada Penderita HIV/ Acquired Immune Deficiency Syndrome (AIDS) Di Kabupaten Sidoarjo Andi Jayawardhana; Harnida, Hanna; Dewi Purwatiningsih; Heni Puspitasari; Mufasirin; Lucia Tri Suwanti; Arif Rahman Nurdianto
NERSMID : Jurnal Keperawatan dan Kebidanan Vol. 6 No. 1 (2023): Mei
Publisher :

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55173/nersmid.v6i1.133

Toxoplasma gondii is an obligate intracellular parasite that can cause toxoplasmosis infection. About 60% of the world's population has been infected. Toxoplasma cerebri is one of the most common opportunistic infections in the central nervous system of HIV patients. The disease is acute and chronic, with mild symptoms that are non-specific so it is difficult to distinguish from other diseases or is often asymptomatic. The incidence of Human Immunodeficiency Virus (HIV) occupies the highest place in Asia with high morbidity and mortality. Toxoplasma is ranked in the top 10 opportunistic diseases as a direct cause of morbidity and mortality, with variable symptoms and reactivation. The purpose of the study: to detect Toxoplasma gondii from blood samples of patients with HIV/Acquired Immune Deficiency Syndrome (AIDS). In immunocompromised conditions, especially HIV/AIDS, symptoms of severe complications will arise, one of which is toxsoplasma encephalitis (TE). The high rate of morbidity and mortality of TE in HIV/AIDS patients, around 30-40%, makes an accurate diagnosis important. Research Methods: The study design used was cross sectional to determine the level of IgG Toxoplasma Gondii antibodies in HIV patients and serologically. The research subjects were 30 individual subjects who were positive for HIV infection, of which 30 patients would be screened for the levels of IgG Toxoplasma Gondii antibodies and then see the correlation with lifestyle and food consumed. The achievement target of this research is to find out whether HIV patients have levels of IgG Toxoplasma Gondii antibodies through serological tests, so that appropriate therapy and education can be given for the treatment of toxoplasmosis, so that these results can be published scientifically in national journals, and become paper material in scientific meetings.

Co-Authors Agung Sosiawan Agustiningsih, Linda Ahmad Yudianto Ahmad Yudianto Aksono HP., Eduardus Bimo Alasrorik, Muhammad Hizbulloh Andi Jayawardhana andi jayawardhana Arif Rahman Nurdianto Boedi Setiawan Budi Koestoro Caswita Caswita Christian Marco Hadi Nugroho Citraningrum, Dina Merdeka Dewi Purwatiningsih Dian Agustin Wahjuningrum Dikman, Dicky Mohammad DWI PUTRI RAHMAWATI Eka Pramyrtha Hestianah Endang Suprihati Erma Safitri Fauziah Fitri Hernanto Fery Setiawan Galaxy Guardian Hani Plumeriastuti Hanna Harnida, Hanna Indasari, Elly Nur jayawardhana, andi Jenny Sunariani Kharisma, Yulan Ayu Krisdianti, Cucuk Kurnia, Ryan Septa Kurniawan, Muhammad ‘Ahdi Kusnoto Laura Wihanto Lucia Suwanti, Lucia Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Meles, Dewa Ketut Mufa, Ramy Inas Mahirah Mufasirin Nugroho, Christian Marco Hadi Nurdianto, Arif Rahman Poedji Hastutiek Pratama, Ponasari Galuh Pudji Srianto Puput, Sesa Putera, Shufi Syahbana Rahadju Ernawati Retno Yuli Rina Vitriasari Ryan Septa Kurnia Safira Iqlima Sarah Sarah, Safira Iqlima Seagames Waluyo, Seagames Septian Hakim Susantoputro Setiawan Koesdarto Setyabudi Setyabudi Sri Pantja Madyawati, Sri Pantja Sulistyawati, Endah Supriyadi Supriyadi Swadheena Patro Tedyanto, Cecilia Putri Tita Damayanti Lestari Tony Hartono Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wardhana, Dhandy Koesoemo Waworuntu, Gladdy L. Widya Paramita Lokapirnasari Yuliana Praptiwi, Yuliana Yunus, Muchammad

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