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Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG 1 Museyaroh Museyaroh; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 28, No 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1725

Abstract

In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted betweenhumans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in variouscountries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused byCoronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid andaccurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test thatdetects the presence of SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of the methods used for this test isChemiluminescent Immunoassay (CLIA). This study aimed to determine the reliability of CLIA. The study was conductedfrom August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination atHusada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCRresults, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnosticsensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%,and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positivepredictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a highersensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG.However, the positive, negative predictive value and efficiency values were the same for IgM and IgG.
Validity of Chemiluminescent Immunoassay Serology Test for Anti-Sars Cov-2 Antibodies IgM and IgG 1 Museyaroh Museyaroh; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 1 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i1.1725

Abstract

In December 2019, an outbreak of acute pneumonia occurred in Wuhan, China. The disease was transmitted between humans through droplets (coughing or sneezing) of infected patients, causing this outbreak to spread rapidly in various countries in the world, including Indonesia. On February 11, 2020, WHO announced the pneumonia was caused by Coronavirus Disease 2019 (COVID-19), which was caused by a new type of Coronavirus, the SARS-CoV-2. A rapid and accurate diagnosis is critical for the control of the COVID-19 outbreak. The widely used test is a serology-based test that detects the  presence of SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of the methods used for this test is Chemiluminescent Immunoassay (CLIA). This study aimed to determine the reliability of CLIA. The study was conducted from August to September 2020. The number of samples was 63 patients' serum. Polymerase chain reaction examination at Husada Utama Hospital, Surabaya, revealed that 21 patients were confirmed positive for COVID-19 with positive PCR results, and 42 patients were healthy with negative COVID-19 results. The results showed that IgM had a diagnostic sensitivity of 85.7%, diagnostic specificity of 92.8%, a positive predictive value of 85.7%, a negative predictive value of 92.8%,and accuracy of 90.4%. In comparison, IgG had a diagnostic sensitivity of 90.4%, diagnostic specificity of 90.4%, a positive predictive value of 82.6%, a negative predictive value of 90.5%, and accuracy of 90.4%. In conclusion, IgG has a higher sensitivity than IgM, while IgM had higher specificity, positive predictive value, and negative predictive value than IgG. However, the positive, negative predictive value and efficiency values were the same for IgM and IgG.
Pemeriksaan Status Gizi dan Kadar Asam Urat Lansia di Posyandu Abiyoso Polkesbaya Evy Diah Woelansari; Juliana Christyaningsih; Suhariyadi Suhariyadi; Lully Hanni Endarini; Museyaroh Museyaroh; Diah Titik Mutiarawati; Wisnu Istanto; Suliati Suliati; Anik Handayati; Sri Sulami Endah Astuti; Pestariati Pestariati; Retno Sasongkowati; Mamik Mamik; Sri Utami; Minarti Minarti; Luthfi Rusyadi; Liliek Soetjiatie
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 2 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 2 Agustus 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i2.13417

Abstract

Proses penuaan dapat memengaruhi kesehatan fisik lansia dan meningkatkan risiko berbagai gangguan kesehatan, termasuk hipertensi dan asam urat. Kegiatan ini berlangsung pada tanggal 14 Juni 2023 bertempat di Balai RW 2 Kelurahan Kertajaya, Surabaya dan dihadiri 59 lansia yang berdomisili di RW 1 dan RW 2 Kelurahan Kertajaya Kecamatan Gubeng Surabaya. Jenis kegiatan yang dilaksanakan adalah pengukuran status gizi, asam urat dan senam hipertensi. Hasil pemeriksaan mengungkapkan 57,6% lansia memiliki kadar asam urat yang tinggi dan mayoritas memiliki kelebihan berat badan, sehingga lansia perlu mengendalikan tekanan darah dengan pengobatan yang diresepkan oleh dokter, perubahan gaya hidup seperti diet rendah garam, olahraga teratur, dan menghindari stres.
Pemeriksaan Kesehatan Warga Panti Yayasan Cinta Kasih Ibu Teresa, Sambikerep, Surabaya Juliana Christyaningsih; Kiaonarn Ongko Waluyo; Museyaroh Museyaroh; Nuning Marina Pengge; Lully Hani Endarini; Suliati Suliati; Nurul Hindaryani
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 1 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 1 April 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i1.8998

Abstract

Yayasan Cinta Kasih Ibu Teresa Sambikerep, Surabaya, Indonesia, menerima penitipan anak, khususnya anak terlantar dan lansia, berjumlah 48 orang terdiri dari 18 lansia, 20 anak, 10 remaja, sedangkan pengelola Yayasan Cinta Kasih sejumlah 6 orang. Sejak masa pandemi, anak dan lansia di Yayasan Cinta Kasih Ibu Teresa tersebut jarang mendapat layanan kesehatan dan keterbatasan ruanggerak anak-anak. Tim Pengabdi Polkesbaya mempunyai solusi yaitu anak di Yayasan Cinta Kasih Ibu Teresa akan mendapat pemeriksaan tumbuh kembang anak, Penyuluhan Hidup Bersih dan Sehat, pemeriksaan Status Gizi, penyuluhan cara menyikat gigi yang benar, sedang untuk dewasa dan lansia adalah pelayanan kesehatan berupa pemeriksaan tekanan darah, gula darah dan asam urat. Hasil yang didapat adalah Penyuluhan oleh tim pengabdi diikuti dengan baik dan antusias oleh warga Yayasan Cinta Kasih Ibu Teresa. Status gizi dan tumbuh kembang anak dan bayi di Yayasan Cinta Kasih Ibu Teresa didapatkan anak kategori kurus 3%, gemuk 9%, obesitas dan BB kurang masing2 3% dan BB lebih 6%, sedangkan 6% dewasa/ lansia didapatkan kadar glukosa darah dan asam urat tinggi. Disarankan kegiatan serupa dapat dilakukan secara periodik agar warga Yayasan Cinta Kasih Ibu Teresa dapat terpantau status kesehatannya.
Interactive Support in The Tambak Wedi Village Area of Surabaya City to Avoid Stunting Christyaningsih, Juliana; Nur Kholifah, Siti; Ariyanti, Ida; Retno Puspitadewi, Teresia; Suyanto, Beny; Hindaryani, Nurul; Rahmawati, Laili; museyaroh, museyaroh
Frontiers in Community Service and Empowerment Vol. 3 No. 3 (2024): September
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v3i3.74

Abstract

ABSTRACT Stunting remains a serious challenge for Indonesian society. The 2018 basic health research reported that the prevalence of stunting among toddlers reached 30.8%. The impact of stunting is not only on physical growth but also on cognitive development and the risk of diseases in adulthood. Community service plays a crucial role in supporting government efforts to address stunting through nutrition education, infant and child feeding practices, sanitation, hygiene, and family economic empowerment. Based on this, the involvement of higher education institutions is necessary to improve public health through an approach of interprofessional collaboration and interprofessional education designed in community service activities that focus on empowering communities through integrative assistance. This community service is carried out in 2024, located in Tambak Wedi Village, Surabaya. The activities conducted include training and assistance in the production of processed foods based on moringa leaves and fish, health education on healthy living behaviors, guidance on parenting and breastfeeding/MPASI, training for early detection of high-risk pregnancies for families, and checking hemoglobin and protein levels for pregnant women in a state of chronic energy deficiency who receive supplementary feeding based on moringa leaves and fish. At the beginning of the community service activities, the results of health and laboratory examinations were as follows: several mothers were found to have a mid-upper arm circumference (MUAC) below 23.5 cm, which may indicate a risk of chronic energy deficiency (CED). Pregnant women were still found with hemoglobin levels below 11 g/dL, indicating anemia during pregnancy. Additionally, protein levels in pregnant women were found to be 6.2 g/dL, and there was one stunted child and seven children in a pre-stunting condition. Following up on the results of the initial health examination, interventions were carried out for pregnant women with chronic energy deficiency and toddlers at risk of stunting by providing snacks made from moringa and fish for two weeks. The Surabaya Hebat cadres also received a refresher on early detection screening for high-risk pregnant women INDEX TERMS Stunting, Moringa leaves and fish, Tambak Wedi
Skrining Antibodi IgM & IgG Toksoplasmolisis pada Ibu Hamil di Puskesmas Kabupaten Sampang Novikasari, Caturizkyana; Suhariyadi, Suhariyadi; Woelansari, Evy Diah; Museyaroh, Museyaroh
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 18 No 2 (2023): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v18i2.1839

Abstract

Latar Belakang: Toksoplasmosis adalah kelainan yang ditimbulkan akibat terjadinya infeksi Toxoplasma gondii. Infeksi toksoplasmosis dapat terjadi secara vertikal, dari ibu pada calon bayi melalui plasenta, sehingga dapat menyebabkan keguguran, kecacatan ataupun lahir mati. Tujuan penelitian ini adalah untuk mengetahui adanya antibodi IgM & IgG toksoplasmosis pada ibu hamil di Puskesmas Torjun Kabupaten Sampang. Metode: Penelitian ini menggunakan metode immunokromatografi kualitatif dengan hasil positif/negatif. Alat pemeriksaan yang digunakan yaitu cassette rapid test Dalf Toxo IgM/IgG. Sampel pemeriksaan yang digunakan berjumlah 25 serum ibu hamil yang melakukan periksa rutin di Puskesmas Torjun. Hasil: Pemeriksaan yang telah dilakukan terhadap 25 sampel darah ibu hamil di Puskesmas Torjun diperoleh sebanyak 25 (100%) ibu hamil negatif IgM toksoplasma. Ibu hamil dengan antibodi IgG toksoplasma positif sebanyak 5 (20%). Ibu hamil dengan antibodi IgG toksoplasma negatif diperoleh sebanyak 20 (80%). Kesimpulan: Berdasarkan hasil penelitian, ibu hamil di Puskesmas Torjun Kabupaten Sampang yang memiliki antibodi IgG toksoplasma positif sebesar 20% tanpa gejala selama kehamilan.
PERBEDAAN MORFOLOGI SEL DARAH PADA PEMERIKSAAN HAPUSAN DARAH TEPI DENGAN PEWARNAAN GIEMSA MENGGUNAKAN LARUTAN PENGENCER BUFFER PHOSPHAT DAN LARUTAN PENGENCER AQUABIDEST Museyaroh, Museyaroh; Nabilah, Musholli Himmatun; Endarini, Lully Hanni
JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan P-ISSN : 1979-3340 e-ISSN : 2685-7987 Vol 16 No 2 (2024): JUNI
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/job.v16i2.2060

Abstract

Pemeriksaan Hapusan darah tepi (HDT) merupakan pemeriksaan untuk melihat morfologi sel-sel darah secara mikroskopis. Salah satu metode pemeriksaan hapusan darah tepi yang sering digunakan adalah pewarnaan giemsa. Pengenceran larutan giemsa secara teoritis menggunakan larutan penyangga buffer phosphat. Pengenceran giemsa sering menggunakan larutan buffer phosphat karena buffer phosphat mudah didapatkan, namun harganya cukup mahal. Kandungan phosphat yang melebihi batas 2 mg/L juga dapat berpengaruh terhadap keseimbangan ekosistem perairan sehingga dapat mencemari lingkungan bila terus digunakan. Penelitian ini bertujuan untuk menganalisis perbedaan morfologi sel-sel darah pada pemeriksaan HDT dengan pewarnaan giemsa menggunakan larutan buffer dan aquabidest dalam upaya penentuan larutan alternatif yang bisa digunakan sebagai larutan pengencer giemsa yang lebih murah dan mudah didapatkan. Penelitian ini merupakan penelitian eksperimental dengan studi cross sectional yang membandingkan kualitas morfologi sel darah sediaan HDT dengan pewarna giemsa yang diencerkan dengan buffer phosphate dan aquabidest. Sampel diambil dari 1 orang koresponden penelitian, untuk kemudian dibuat sediaan HDT masing-masing sebanyak 17 buah (pengencer buffer phosphat), dan 22 buah (pengencer aquabidest), dan diamati dengan mikroskop perbesaran lensa objektif 100x. Kualitas sediaan HDT giemsa dengan pengencer buffer phosphat menunjukkan hasil “baik” sebanyak 13 buah (76,47%), dan hasil “kurang baik” sebanyak 4 buah (23,53%), sedangkan kualitas sediaan HDT giemsa dengan pengencer aquabidest menunjukkan hasil “baik” sebanyak 18 buah (81,82%), dan hasil “kurang baik” sebanyak 4 buah (18,18%). Berdasarkan uji Mann-Whitney U, didapatkan P-Value 0,222. Kualitas sediaan HDT pewarnaan giemsa dengan pengencer buffer phosphat tidak menunjukkan perbedaan yang signifikan dengan yang menggunakan pengencer aquabidest (p>0,05).
Correlation between ESR and hs-CRP Levels with HbA1c in diabetes mellitus patients Farah Adelia Qotrunnada; Anik Handayati; Museyaroh; Lully Hanni Endarini
JHeS (Journal of Health Studies) Vol. 8 No. 2 (2024): September
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/jhes.3747

Abstract

Subclinical inflammation and almost every indication of systemic inflammation, which is defined by elevated levels of inflammatory markers in the blood, are seen in patients with diabetes mellitus (DM). Understanding the relationship between inflammatory markers and glycemic control is essential for creating comprehensive management strategies to avoid diabetes-related complications. The aims of this study were to determine the correlation between erythrocyte sedimentation rate (ESR) and high-sensitivity C-reactive protein (hs-CRP) and glycated hemoglobin (HbA1c) in DM patients. This was an analytical observational study with a cross-sectional design that was conducted on 35 DM patients from the DM prolanis group at the Bangkalan Regency First-Level Health Facility. HbA1c and hs-CRP levels were measured using the fluorescence immunoassay (FIA) method, while ESR was assessed using the Westergren method. Statistical analysis was performed using the Spearman correlation test. Regarding the biomarkers, all respondents (100%) had HbA1c levels >8.0%, 43% had elevated hs-CRP levels associated with cardiovascular risk, and 80% had abnormal ESR values. A significant relationship was found between ESR and HbA1c (p = 0.019; r = 0.394), but no significant relationship was observed between hs-CRP and HbA1c (p = 0.351; r = 0.163). The relationship between inflammation and glycemic control in DM can be understood through the correlation of ESR, hs-CRP, and HbA1c. These findings offer practical value, particularly in monitoring disease progression and managing diabetes-related complications. Future research should investigate the effects of interventions aimed at reducing inflammation, e.g lifestyle modifications or anti-inflammatory medications, on ESR, hs-CRP, and HbA1c levels.
Variance Index Score (Vis) Evaluation Of Homemade And Commercial Control Sera On Ast And Alt Assays Rahmania, Adelia Prisma; Handayati, Anik; Museyaroh, Museyaroh; Haryanto, Edy
Jurnal Riset Kesehatan Vol 13, No 2 (2024): NOVEMBER 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrk.v13i2.11778

Abstract

Control materials are used as indicators of monitoring the performance of laboratory examinations so that the results issued are valid. Method of assessing the implementation of External Quality Assurance (EQAS) used Variance Index Score (VIS). This study aims to determine difference between pooled sera, homemade and commercial lyophilizates on AST and ALT parameters based on VIS. This research used comparative descriptive with quantitative approach in December 2023-May 2024. Homemade lyophilizates performed at UBAYA technobiology laboratory, determination of true value performed at 2 reference laboratories and clinical chemistry laboratory Poltekkes Surabaya, and determination of VIS performed at 10 primary level clinical laboratories in Surabaya Raya which determined by purposive sampling. The statistical analysis results showed no difference between homemade and commercial control materials based on VIS to the true value and all participating laboratories, so it was concluded that homemade control materials could be used as a substitute in implementing EQAS.
Kolesterol dan Tekanan Darah Lansia di Posyandu Abiyoso Polkesbaya Christyaningsih, Juliana; Rusyadi, Luthfi; Minarti, Minarti; Mamik, Mamik; Pestariati, Pestariati; Mutiarawati, Diah Titik; Suliati, Suliati; Sasongkowati, Retno; Woelansari, Evy Diah; Suhariyadi, Suhariyadi; Istanto, Wisnu; Endarini, Lully Hanni; Museyaroh, Museyaroh
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 2 No 2 (2023): Jurnal Rakat Sehat: Pengabdian Kepada Masyarakat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v2i2.35

Abstract

As people age, the physical state of the human body will gradually deteriorate due to a decrease in the quantity of cells present, leading to a gradual reduction in functionality referred to as the aging process. In elderly humans (aged more than 60 years) excessive cholesterol levels and high blood pressure are one of the causes of diseases such as heart disease which has been recognized by WHO as the number one cause of death in the world. The buildup of fatty deposits on the linings of blood vessels has the potential to lead to obstructions within the blood vessels. The objective of this initiative is to enhance understanding regarding the hazards associated with cholesterol. This outreach effort took place in July 2023, conducted at Posyandu Abiyoso, Surabaya Health Polytechnic. The activity consists of several processes 1). conducting a survey of the location of the Abiyoso posyandu of the Surabaya Health Polytechnic, 2). Opening registration and taking participants' personal data, 3) conducting participant health interviews, 4). Checking cholesterol levels and blood pressure of participants. From the community service activities carried out, it was found that 42 of the 65 (64.6%) participants who took part in the examination at the integrated service post had high cholesterol levels, and 60 of the 65 (92.3%) participants had high blood pressure or hypertension. By conducting health checks on cholesterol levels and blood pressure, there is an expectation that the society will come to realize and comprehend. the dangers of hypertension and hypercholesterolemia so that the community can prevent the occurrence of sustainable diseases.
Co-Authors ., Pestariati Amalia Putri Khurota Ayyun Amania, Salsabila Anik Handayati Anik Handayati Anita Dwi Anggraini Ardani, Nandita Diva Aryati Aryati Aryati Aryati Briansky, Sabira Alvayogi Deddy Adam Diah Titik Mutiarawati Dwi Kriharyani Dwi Rahayu Edy Haryanto Edy Haryanto Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hanni Era Fitria Yunita Ersanto, Novi Evy Diah Woelansari Evy Diah Woelansari Evy Diah Woelansari Evy Dyah Woelansari Fa'iqotul Azharil Jauharoh Farah Adelia Qotrunnada Handayati, Anik Haqqi, Faishol Hindaryani, Nurul Ida Ariyanti, Ida Ilma Ainun Nisa Isnaini, Dian Istanto, Wisnu Juliana Christyaningsih Kiaonarn Ongko Waluyo Kriharyani, Dwi Kristiningsih, Elly Kuswanto, Muhammad Kuswidyaningrum, Desviona Laili Rahmawati, Laili Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Mamik Mamik Masdiva Putri Hidayah Maulida Azmi Wimasni Inah Maulida, Mas'ulatur Rohmah Minarti Minarti Minarti Minarti Musholli Himmatun Nabilah Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Novikasari, Caturizkyana Novita, Devy Nuning Marina Pengge Nur Kholifah, Siti Puspa Wardani Puspa Wardhani Puspitasari, Erlita Putri , Anggi Ananta Rahmania, Adelia Prisma Ratno Tri Utomo Retno Puspitadewi, Teresia Retno Sasongkowati Retno Sasongkowati Retno Werdiningsih Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sasongkowati, Retno Shella Nur Laily Sri Utami Sugihani Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Suyanto, Beny Toni Watoyani Trisnania, Iid Dani Wisnu Istanto Woelansari, Evy Dyah