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Raudhotun Jamila
Universitas Jember
Agriculture, Biological Sciences & Forestry

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PENGARUH SAMPAH ORGANIK TERHADAP KARAKTERISTIK KIMIA VERMIKOMPOS Ahmad Ilham Tanzil; Puji Rahayu; Raudhotun Jamila; Wahyu Indra Duwi Fanata; Ummi Sholikhah; Tri Ratnasari
AGRORADIX : Jurnal Ilmu Pertanian Vol 7 No 1 (2023): Desember 2023
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v7i1.5262

Abstract

Vermicompost is a fertilizer produced by composting organic matter by utilizing the interaction between earthworms and microorganism. Vermicompost also contains a number of macro and micro nutrients needed by plants. Besides containing macro and micro of nutrients, vermicompost also containing growth hormone such as cytokinins and auxins which are produced by worms during the composting process. The benefits of vermicompost are that it can improve the physical, chemical and biological properties of soil, increase the ability of the soil to retain water, rebuild damaged soil structures and increase ability of the soil to exchange cations. The fermentation process lasted for 14 days using Lumbricus rubellus worms. The choice of this type worm is based on its character which has a high level of productivity, can eat all types of organic matter, is much more adaptive and move less actively. This research was held in Sumbersalak Village, Ledokombo District, Jember Regency and also involving the Kenconowungu farmer group. Goat manure become waste in this village so it has potential to be developed further as the main ingredient for vermicompost. The other additional materials such as vegetable and fruits waste, and leaf litter were used as factor (treatment). These three types of materials are used to find out which treatment gives the best vermicompost quality. Chemical analysis was carried out in this study to determine the organic N, P, K, C organic, pH, and water content. The result of the analysis will be compared with the standards of Minister of Agriculture No.70 of 2011.
Pengaruh Metode Isolasi DNA terhadap Kuantitas dan Kualitas Hasil Ekstraksi Genom Padi dan Tebu Ahmad Ilham Tanzil; Intan Ria Neliana; Wahyu Indra Duwi Fanata; Raudhotun Jamila; Bambang Sugiharto; Ummi Sholikhah; Tri Ratnasari
AGRORADIX : Jurnal Ilmu Pertanian Vol. 9 No. 2 (2026): Januari-Juli 2026
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

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Abstract

Teknik isolasi DNA merupakan salah satu metode dasar dalam bidang biologi molekuler yang bertujuan untuk memisahkan DNA dari komponen seluler lainnya. Memperoleh DNA berkualitas tinggi dengan hasil yang baik merupakan faktor pembatas dalam analisis genetik tanaman, karena sulit untuk memperoleh DNA berkualitas tinggi dalam jumlah yang memadai. Penelitian ini kami lakukan untuk mengoptimalkan teknik isolasi DNA genomik dari sampel tanaman, dengan tujuan memastikan kualitas dan kuantitas DNA yang diisolasi cukup untuk analisis molekuler. Metode yang digunakan melibatkan perbandingan teknik ekstraksi menggunakan metode mekanis dan metode gerus. Daun tebu dan padi, baik dalam kondisi basah maupun kering, digunakan sebagai sampel. Hasil penelitian menunjukkan peningkatan signifikan dalam konsentrasi dan kualitas DNA saat sampel basah digiling menggunakan proses gerus. Berdasarkan perbandingan absorbansi pada A260/A280 nm dan A260/A230 nm, serta hasil uji nanodrop, proses penggerusan menghasilkan kualitas dan konsentrasi DNA yang lebih tinggi. Dibandingkan dengan metode mekanis, hasil visualisasi PCR menunjukkan bahwa metode gerus dapat mengekspresikan pita DNA yang lebih tebal pada sampel daun padi. Sementara itu, terdapat beberapa variasi dalam ekspresi pita DNA pada sampel daun tebu. Data kuantitatif dihasilkan dari temuan visualisasi PCR. Metode gerus terbukti menghasilkan jumlah dan kualitas DNA sampel terbaik, berdasarkan hasil penelitian.
Co-Authors Ahmad Ilham Tanzil Bambang Sugiharto Neliana, Intan Ria Puji Rahayu Tri Ratnasari Ummi Sholikhah Wahyu Indra Duwi Fanata