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All Journal Jurnal ABDINUS : Jurnal Pengabdian Nusantara ZIRAA'AH MAJALAH ILMIAH PERTANIAN Didaktika: Jurnal Kependidikan Life Science
Siti Khotimah
Universitas Tanjungpura
Agriculture, Biological Sciences & Forestry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mathematics Other

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Journal : life science

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Viabilitas Oospora Peronospora manshurica Asal Kacang Kedelai (Glycine max) Impor Berdasarkan Perbedaan Suhu Inkubasi Muhammad Sunariya; Siti Khotimah; Ngaisatul Lutfiyah
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.69480

Abstract

Soybean (Glycine max) is the highest imported commodity entering West Kalimantan which has the potential to carry pathogens such as Peronospora manshurica. P. manshurica is a pathogen on soybean plants that causes downy mildew disease which can reduce the productivity of soybean plants. This fungus can spread with propagules in the form of oospores and mycelium on the seed coat like a grayish-white crust which can live if environmental conditions are suitable, such as temperature factors. The purpose of this study was to determine the viability of P. manshurica oospores tested with Triphenyl Tetrazolium Chloride based on differences in incubation temperature. This study used 240 symptomatic soybean seeds which were given three levels of incubation temperature treatment at 20oC, 30oC and 40oC with 8 repetitions using a completely randomized design. The oospore viability values were analyzed by one way ANOVA showing there were significant differences in each treatment. The viability values of live oospores obtained were 28.21% at 20oC, 17.06% at 30oC and 7.6% at 40oC. The highest viability value of live oospores was obtained at 20oC and showed that the higher the incubation temperature, the lower the viability value of P. manshurica oospores.
Sinergisme Isolat Potensial Biofertilizer Bacillus cereus yang Dikonsorsiumkan Muhammad Fajar; Siti Khotimah; Rahmawati Rahmawati
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.73792

Abstract

A consortium of microorganisms is a mixture of microbial populations in the form of a community that has cooperative, commensal, and mutualistic relationships. Naturally, bacteria are able to communicate with each other. The relationship between bacteria can have a synergistic relationship if the substrate is sufficient and the bacteria do not inhibit each other's growth. In this study, Bacillus cereus isolates SP1, SP2, and SP3 isolated from Sungai Ambawang peatland, Kubu Raya Regency, West Kalimantan will be consorted. The purpose of this study was to determine the synergism of three strains of Bacillus cereus bacteria. All isolates tested for synergism against other isolates showed no inhibition zone at the intersection between isolates. This means that each isolate can synergize with one another. The synergism test results show that three strains of Bacillus cereus bacteria can synergize in the manufacture of biofertilizers.
Co-Authors Achmadi Aotza Lisandra Firman Saputra Istiqamah Istiqamah Maria Ulfah Masnur Turnip Muhammad Fajar Muhammad Sunariya Mukarlina -, Mukarlina Ngaisatul Lutfiyah Rahmawati Rahmawati Rody Putra Sartika Usman Radiana Zulfa Zakiah